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1.
Can Vet J ; 59(9): 988-992, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-30197442

RESUMO

This study tested the hypothesis that the presence of prostaglandin E2 in seminal plasma would aid in the transport of phenolsulfonphthalein (PSP) across the uterotubal junction. Five mares in estrus were inseminated during estrus with PSP dissolved in phosphate-buffered saline and during the subsequent estrus with PSP added to a standard insemination dose. Serum and urine samples were obtained at hours 0, 1, 2, and 3 following treatment and examined for the presence of PSP. Phenolsulfonphthalein could not be detected in any of the urine samples collected from mares following either treatment. None of the serum samples collected following intrauterine installation of PSP in PBS contained PSP. Phenolsulfonphthalein was detected in serum samples from 1 mare following insemination with semen containing PSP. Components in seminal plasma such as PGE2 did not facilitate the transport of PSP across the uterotubal junction as had been hypothesized.


Le plasma séminal ne facilite pas le transport de la phénolsulfonphtaléine au travers de la jonction utéro-tubaire des juments. Cette étude a testé l'hypothèse voulant que la présence de la prostaglandine E2 dans le plasma séminal facilite le transport de la phénolsulfonphtaléine (PSP) au travers de la jonction utéro-tubaire. Cinq juments en oestrus ont été inséminées avec de la PSP dissoute dans une solution saline tamponnée au phosphate et, durant l'oestrus subséquent, avec de la PSP ajoutée à une dose d'insémination standard. Des prélèvements de sérum et d'urine ont été obtenus aux heures 0, 1, 2 et 3 ainsi qu'après le traitement et examinés pour déceler la présence de la PSP. La phénolsulfonphtaléine n'a pas pu être détectée dans aucun des échantillons d'urine prélevés auprès des juments après l'un ou l'autre des traitements. Aucun des échantillons de sérum prélevés après l'installation intra-utérine de la PSP dans PBS ne contenait de PSP. La phénolsulfonphtaléine a été détectée dans des échantillons de sérum provenant d'une jument après l'insémination avec du sperme contenant de la PSP. Des composants dans le plasma séminal comme le PGE2 n'ont pas facilité le transport de la PSP au travers de la jonction utéro-tubaire conformément à l'hypothèse émise.(Traduit par Isabelle Vallières).


Assuntos
Doenças dos Anexos/veterinária , Doenças dos Cavalos/diagnóstico , Fenolsulfonaftaleína/administração & dosagem , Doenças dos Anexos/diagnóstico , Animais , Dinoprostona , Estro , Feminino , Cavalos , Inseminação Artificial/veterinária , Masculino , Oviductos/fisiopatologia , Fenolftaleínas/sangue , Fenolftaleínas/urina , Fenolsulfonaftaleína/análise , Sêmen/química
2.
Water Sci Technol ; 73(3): 628-35, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26877046

RESUMO

When assigning site-specific restoration targets for deteriorating aquatic systems, it is necessary to have an understanding of the undisturbed or background state of the system. However, the site-specific characteristics of aquatic systems prior to disturbance are mostly unknown, due to the lack of historical water and sediment quality data. This study aims to introduce a method for filling this gap in our understanding, using dated sediment cores from the beds of aquatic environments. We used Bolin Billabong, a floodplain lake of the Yarra River (South-East Australia), as a case study to demonstrate the application of this method. We identified the concentrations of aluminium, cadmium, chromium, copper, iron, lead, manganese, nickel, tin and zinc at 8 cm intervals through the sediment core. This showed that aluminium, chromium, copper, iron, lead, nickel, tin and zinc concentrations in Bolin Billabong sediments significantly increased after European settlement in the river catchment in the mid-19th century. The differences between current Australian sediment quality guidelines trigger values and the background metal concentrations in Bolin Billabong sediments underscore the value of using locally relevant background toxicant concentrations when setting water and sediment quality targets.


Assuntos
Monitoramento Ambiental/métodos , Recuperação e Remediação Ambiental , Sedimentos Geológicos/análise , Lagos/química , Metais/análise , Poluentes Químicos da Água/análise , Fatores de Tempo , Vitória
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