RESUMO
In the multibarrier concept for the deep geological disposal of high-level radioactive waste (HLW), bentonite is proposed as a potential barrier and buffer material for sealing the space between the steel canister containing the HLW and the surrounding host rock. In order to broaden the spectra of appropriate bentonites, we investigated the metabolic activity and diversity of naturally occurring microorganisms as well as their time-dependent evolution within the industrial B25 Bavarian bentonite under repository-relevant conditions. We conducted anaerobic microcosm experiments containing the B25 bentonite and a synthetic Opalinus Clay pore water solution, which were incubated for one year at 30 and 60 °C. Metabolic activity was only stimulated by the addition of lactate, acetate, or H2. The majority of lactate- and H2-containing microcosms at 30 °C were dominated by strictly anaerobic, sulfate-reducing, and spore-forming microorganisms. The subsequent generation of hydrogen sulfide led to the formation of iron-sulfur precipitations. Independent from the availability of substrates, thermophilic bacteria dominated microcosms that were incubated at 60 °C. However, in the respective microcosms, no significant metabolic activity occurred, and there was no change in the analyzed biogeochemical parameters. Our findings show that indigenous microorganisms of B25 bentonite evolve in a temperature- and substrate-dependent manner.
Assuntos
Bentonita , Resíduos Radioativos , Geologia , Ferro , SulfatosRESUMO
The ALLIANCE Strategic Research Agenda (SRA) for radioecology is a living document that defines a long-term vision (20 years) of the needs for, and implementation of, research in radioecology in Europe. The initial SRA, published in 2012, included consultation with a wide range of stakeholders (Hinton et al., 2013). This revised version is an update of the research strategy for identified research challenges, and includes a strategy to maintain and develop the associated required capacities for workforce (education and training) and research infrastructures and capabilities. Beyond radioecology, this SRA update constitutes a contribution to the implementation of a Joint Roadmap for radiation protection research in Europe (CONCERT, 2019a). This roadmap, established under the H2020 European Joint Programme CONCERT, provides a common and shared vision for radiation protection research, priority areas and strategic objectives for collaboration within a European radiation protection research programme to 2030 and beyond. Considering the advances made since the first SRA, this updated version presents research challenges and priorities including identified scientific issues that, when successfully resolved, have the potential to impact substantially and strengthen the system and/or practice of the overall radiation protection (game changers) in radioecology with regard to their integration into the global vision of European research in radiation protection. An additional aim of this paper is to encourage contribution from research communities, end users, decision makers and other stakeholders in the evaluation, further advancement and accomplishment of the identified priorities.
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Monitoramento de Radiação , Proteção Radiológica , Europa (Continente)RESUMO
Bacteria in acidophilic biofilm communities, i.e. acid streamers and snottites, obtained from a subsurface mine in Königstein were visualized by fluorescence microscopy using four new fluorescent dyes (DY-601XL, V07-04118, V07-04146, DY-613). The pH of the bulk solution in which these bacteria thrive was pH 2.6 to 2.9. The new fluorescent dyes were all able to clearly stain and microscopically visualize in-situ the bacteria within the biofilm community without changing pH or background ion concentration. The commonly used fluorescent dyes DAPI and SYTO 59 were also applied for comparison. Both dyes, however, were not able to visualize any bacteria in-situ, since they were not stable under the very acid conditions. In addition, dye V07-04118 and dye DY-613 also possess the ability to stain larger cells which were presumably eukaryotic origin and may be attributed to yeast cells or amoeba-like cells. PCR analyses have shown that the dominant bacterial species in these acidophilic biofilm communities was a gram negative bacterium of the species Ferrovum myxofaciens. The presented four new dyes are ideal for in-situ investigations of microorganisms occurring in very acid conditions, e.g. in acidophilic biofilm communities when in parallel information on pH sensitive incorporated fluorescent heavy metals should be acquired.
Assuntos
Bactérias/metabolismo , Biofilmes , Corantes Fluorescentes/química , Imagem Molecular/métodos , Fenômenos Fisiológicos Bacterianos , Concentração de Íons de Hidrogênio , Metais Pesados/química , Espectrometria de FluorescênciaRESUMO
Biofilms may immobilize toxic heavy metals in the environment and thereby influence their migration behaviour. The mechanisms of these processes are currently not understood, because the complexity of such biofilms creates many discrete geochemical microenvironments which may differ from the surrounding bulk solution in their bacterial diversity, their prevailing geochemical properties, e.g. pH and dissolved oxygen concentration, the presence of organic molecules, e.g. metabolites, and many more, all of which may affect metal speciation. To obtain such information, which is necessary for performance assessment studies or the development of new cost-effective strategies for cleaning waste waters, it is very important to develop new non-invasive methods applicable to study the interactions of metals within biofilm systems. Laser fluorescence techniques have some superior features, above all very high sensitivity for fluorescent heavy metals. An approach combining confocal laser scanning microscopy and laser-induced fluorescence spectroscopy for study of the interactions of biofilms with uranium is presented. It was found that coupling these techniques furnishes a promising tool for in-situ non-invasive study of fluorescent heavy metals within biofilm systems. Information on uranium speciation and uranium redox states can be obtained.
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Biofilmes , Lasers , Urânio/química , Oxirredução , Espectrometria de Fluorescência , Propriedades de Superfície , Urânio/metabolismoRESUMO
Low-temperature alteration reactions on uranium phases may lead to the mobilization of uranium and thereby poses a potential threat to humans living close to uranium-contaminated sites. In this study, the surface alteration of uraninite (UO(2)) and uranium tetrachloride (UCl(4)) in air atmosphere was studied by confocal laser scanning microscopy (CLSM) and laser-induced fluorescence spectroscopy using an excitation wavelength of 408 nm. It was found that within minutes the oxidation state on the surface of the uraninite and the uranium tetrachloride changed. During the surface alteration process U(IV) atoms on the uraninite and uranium tetrachloride surface became stepwise oxidized by a one-electron step at first to U(V) and then further to U(VI). These observed changes in the oxidation states of the uraninite surface were microscopically visualized and spectroscopically identified on the basis of their fluorescence emission signal. A fluorescence signal in the wavelength range of 415-475 nm was indicative for metastable uranium(V), and a fluorescence signal in the range of 480-560 nm was identified as uranium(VI). In addition, the oxidation process of tetravalent uranium in aqueous solution at pH 0.3 was visualized by CLSM and U(V) was fluorescence spectroscopically identified. The combination of microscopy and fluorescence spectroscopy provided a very convincing visualization of the brief presence of U(V) as a metastable reaction intermediate and of the simultaneous coexistence of the three states U(IV), U(V), and U(VI). These results have a significant importance for fundamental uranium redox chemistry and should contribute to a better understanding of the geochemical behavior of uranium in nature.
Assuntos
Compostos de Urânio/química , Urânio/química , Cloretos/análise , Cinética , Espectrometria de Massas , Oxirredução , Espectrometria de Fluorescência , Análise Espectral , Termodinâmica , Compostos de Urânio/análise , Compostos de Urânio/síntese química , Difração de Raios XRESUMO
Synthetically prepared boltwoodite and compreignacite were characterized with time-resolved laser-induced fluorescence spectroscopy (TRLFS). The obtained TRLFS emission spectra of both synthesized uranium minerals differ from each other in their positions of the vibronic peak maxima and in their fluorescence lifetimes. Also, the shapes of the spectra and their respective intensities are different. The TRLFS-spectrum of boltwoodite showed well-resolved sharp vibronic peaks at 485.1, 501.5, 521.2, 543.0, 567.4, and 591.4nm with deep notches between them and compreignacite is characterized by two broad peaks with various shoulders. Here five emission bands were identified at 500.7, 516.1, 532.4, 554.3, and 579.6nm. The shape of the TRLFS spectra of compreignacite is typical for uranium in a hydroxide coordination environment. For both minerals two fluorescence lifetimes were extracted. The two species of boltwoodite and compreignacite, respectively, showed the same positions of the peak maxima showing that the coordination environments are similar, but differ in the chemistry and number of possible quenchers, e.g. water molecules and hydroxide groups. For boltwoodite fluorescence lifetimes of 382 and 2130ns, and for compreignacite shorter ones of 202 and 914ns, respectively, were determined. The spectroscopic signatures of the two uranyl minerals reported here could be useful for identifying uranyl(VI) mineral species as colloids, as thin coatings on minerals, as minor component in soils, or as alteration products of nuclear waste.
Assuntos
Minerais/química , Compostos de Potássio/química , Compostos de Silício/química , Compostos de Urânio/química , Lasers , Espectrometria de Fluorescência/métodosRESUMO
Fluorescence properties of a uranyl(V)-carbonate species in solution are reported for the first time. The fluorescence characteristics of the stable aqueous uranyl(V)-carbonate complex [U(V)O(2)(CO(3))(3)](5-) was determined in a frozen solution (T=153K) of 0.5mM uranium and 1.5M Na(2)CO(3) at pH 11.8 by time resolved laser-induced fluorescence spectroscopy (TRLFS). Two different wavelengths of 255nm and 408nm, respectively were used to independently of each other excite the uranyl(V)-carbonate species. The resulting U(V) fluorescence emission bands were detected between 380nm and 440nm, with a maxima at 404.7nm (excitation with 255nm) and 413.3nm (excitation with 408nm), respectively. It was found that by using an excitation wavelength of 255nm the corresponding extinction coefficient was much higher and the fluorescence spectrum better structured than the ones excited at 408nm. The fluorescence lifetime of the uranyl(V)-carbonate species was determined at 153K as 120micros. TRLFS investigations at room temperature, however, showed no fluorescence signal at all.
Assuntos
Carbonatos/química , Espectrometria de Fluorescência/métodos , Urânio/química , Fluorescência , LasersRESUMO
Uranium(VI) was sorbed to freshly ground and leached albite in batch and flow-through systems in the pH range 5.0-6.4. The uranium(VI) surface complexes were studied by extended X-ray absorption fine structure (EXAFS) spectroscopy and time-resolved laser-induced fluorescence spectroscopy (TRLFS). The EXAFS analysis of uranium(VI) sorbed on albite at pH 5.8 and 5 x 10(-6) M U(VI) showed one silicon atom at a USi distance of 3.09 A, which is indicative of the formation of an inner-sphere, mononuclear, bidentate uranium(VI) surface complex, Si(O)2UO2, on the silicate tetrahedra of albite. Two additional uranium(VI) sorption complexes were detected by TRLFS at higher initial aqueous U(VI) concentrations. However, the structure of these surface complexes could not be derived from EXAFS, since the measured EXAFS spectra represent the average of two surface complex structures. In order to simulate U(VI) sorption onto weathered feldspar surfaces, albite was leached with 0.01 M HClO4, resulting in surface material similar to amorphous silica gel. EXAFS showed that the equatorial oxygen shell of uranium(VI) sorbed on this material at pH 5.0 and 5.8 was split in two distances of 2.23 and 2.44 A. This indicates the formation of an inner-sphere surface complex.
RESUMO
Time-resolved laser-induced fluorescence spectroscopy (TRLFS) was combined with batch experiments to study the sorption of uranium(VI) onto gibbsite (gamma-Al(OH)3). The experiments were performed under ambient conditions in 0.1 M NaClO4 solution in the pH range from 5.0 to 8.5 using a total uranium concentration of 1x10(-5) M, and a solid concentration of 0.5 g/40 ml. Two uranyl surface species with fluorescence lifetimes of 330+/-115 and 5600+/-1640 ns, respectively, were identified. The first species was dominating the more acid pH region whereas the second one became gradually more prominent towards higher pH values. The fluorescence spectra of both adsorbed uranyl(VI) surface species were described with six characteristic fluorescence emission bands situated at 479.5+/-1.1, 497.4+/-0.8, 518.7+/-1.0, 541.6+/-0.7, 563.9+/-1.2, and 585.8+/-2.1 nm. The surface species with the short-lived fluorescence lifetime of 330 ns is attributed to a bidentate mononuclear inner-sphere surface complex in which the uranyl(VI) is bound to two reactive OH- groups at the broken edge linked to one Al. The second surface species with the significant longer fluorescence lifetime of 5600 ns was attributed to small sorbed clusters of polynuclear uranyl(VI) surface species. The longer fluorescence lifetime of the long-lived uranyl surface species at pH 8.5 is explained with the growing average size of the adsorbed polynuclear uranyl surface species.
Assuntos
Hidróxido de Alumínio/química , Hidróxido de Alumínio/efeitos da radiação , Lasers , Urânio/química , Urânio/efeitos da radiação , Adsorção , Sensibilidade e Especificidade , Espectrometria de Fluorescência/métodos , Fatores de TempoRESUMO
This paper presents a digitized version of a thermodynamic sorption database, implemented as a relational database with MS Access. It is mineral-specific and can therefore be used for additive models of complex solid phases such as rocks or soils. An integrated user interface helps users to access selected mineral and sorption data, to extract internally consistent data sets for sorption modeling, and to export them in formats suitable for other modeling software. Data records comprise mineral properties, specific surface area values, surface binding sites' characteristics, sorption ligand information, and surface complexation reactions. An extensive bibliography is included, providing links not only to the above listed data, but also to background information concerning surface complexation model theories, evidence for surface species, and sorption experimental techniques.
Assuntos
Bases de Dados Factuais , Poluentes Ambientais , Geologia , Modelos Teóricos , Radioisótopos , Adsorção , Fenômenos Geológicos , Ligantes , Medição de Risco , TermodinâmicaRESUMO
Uranium concentrations in cultivated (sunflower, sunchoke, potato) and native plants, plant compartment specimens, and mushrooms, grown on a test site within a uranium-contaminated area in Eastern Thuringia, were analyzed and compared. This test site belongs to the Friedrich-Schiller University Jena and is situated on the ground of a former but now removed uranium mine waste leaching heap. For determination of the U concentrations in the biomaterials, the saps of the samples were squeezed out by using an ultracentrifuge, after that, the uranium concentrations in the saps and the remaining residue were measured, using ICP-MS. The study further showed that uranium concentrations observed in plant compartment and mushroom fruiting bodies sap samples were always higher than their associated solid residue sample. Also, it was found that the detected uranium concentration in the root samples were always higher than were observed in their associated above ground biomass, e.g., in shoots, leaves, blossoms etc. The highest uranium concentration was measured with almost 40 ppb U in a fruiting body of a mushroom and in roots of butterbur. However, the detected uranium concentrations in plants and mushrooms collected in this study were always lower than in the associated surface and soil water of the test site, indicating that under the encountered natural conditions, none of the studied plant and mushroom species turned out to be a hyperaccumulator for uranium, which could have extracted uranium in sufficient amounts out of the uranium-contaminated soil. In addition, it was found that the detected uranium concentrations in the sap samples, despite being above the sensitivity limit, proved to be too low-in combination with the presence of fluorescence quenching substances, e.g., iron and manganese ions, and/or organic quenchers-to extract a useful fluorescence signal, which could have helped to identify the uranium speciation in plants.
Assuntos
Agaricales/química , Biodegradação Ambiental , Helianthus/química , Poluentes Radioativos do Solo/análise , Solanum tuberosum/química , Urânio/análise , Agaricales/metabolismo , Alemanha , Helianthus/metabolismo , Espectrometria de Massas , Mineração , Raízes de Plantas/química , Poluentes Radioativos do Solo/farmacocinética , Solanum tuberosum/metabolismo , Urânio/farmacocinética , ResíduosRESUMO
In situ leaching of uranium ores with sulfuric acid during active uranium mining activity on the Gessenheap has caused longstanding environmental problems of acid mine drainage and elevated concentrations of uranium. To study there remediation measures the test site Gessenwiese, a recultivated former uranium mining heap near Ronnenburg/East Thuringia/Germany, was installed as a part of a research program of the Friedrich-Schiller University Jena to study, among other techniques, the phytoremediation capacity of native and selected plants towards uranium. In the first step the uranium speciation in surface seepage and soil pore waters from Gessenwiese, ranging in pH from 3.2 to 4.0, were studied by time-resolved laser-induced fluorescence spectroscopy (TRLFS). Both types of water samples showed mono-exponential luminescence decay, indicating the presence of only one major species. The detected emission bands were found at 477.5, 491.8, 513.0, 537.2, 562.3, and 590.7 nm in case of the surface water samples, and were found at 477.2, 493.2, 513.8, 537.0, 562.4, and 590.0 nm in case of the soil water samples. These characteristic peak maxima together with the observed mono-exponential decay indicated that the uranium speciation in the seepage and soil pore waters is dominated by the uranium (VI) sulfate species UO2SO4(aq). Due to the presence of luminescence quenchers in the natural water samples the measured luminescence lifetimes of the UO2SO4(aq) species of 1.0-2.6 µs were reduced in comparison to pure uranium sulfate solutions, which show a luminescence lifetime of 4.7 µs. These results convincingly show that in the pH range of 3.2-4.0 TRLFS is a suitable and very useful technique to study the uranium speciation in naturally occurring water samples.
RESUMO
The underground uranium mine Königstein (Saxony, Germany), currently in the process of remediation, represents an underground acid mine drainage (AMD) environment, that is, low pH conditions and high concentrations of heavy metals including uranium, in which eye-catching biofilm formations were observed. During active uranium mining from 1984 to 1990, technical leaching with sulphuric acid was applied underground on-site resulting in a change of the underground mine environment and initiated the formation of AMD and also the growth of AMD-related copious biofilms. Biofilms grow underground in the mine galleries in a depth of 250 m (50 m above sea level) either as stalactite-like slime communities or as acid streamers in the drainage channels. The eukaryotic diversity of these biofilms was analyzed by microscopic investigations and by molecular methods, that is, 18S rDNA PCR, cloning, and sequencing. The biofilm communities of the Königstein environment showed a low eukaryotic biodiversity and consisted of a variety of groups belonging to nine major taxa: ciliates, flagellates, amoebae, heterolobosea, fungi, apicomplexa, stramenopiles, rotifers and arthropoda, and a large number of uncultured eukaryotes, denoted as acidotolerant eukaryotic cluster (AEC). In Königstein, the flagellates Bodo saltans, the stramenopiles Diplophrys archeri, and the phylum of rotifers, class Bdelloidea, were detected for the first time in an AMD environment characterized by high concentrations of uranium. This study shows that not only bacteria and archaea may live in radioactive contaminated environments, but also species of eukaryotes, clearly indicating their potential influence on carbon cycling and metal immobilization within AMD-affected environment.
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The alteration of a depleted uranium (DU) disk in contact with a synthetic pore water, as a simulantforfertilized agricultural soil, was studied by exposing the DU to a calcium phosphate solution (2.5 x 10(-3) M Ca, 1 x 10(-3) M P). Within 12 months this contact resulted in the formation of a thin film of a secondary uranium mineral on the metallic DU. The reaction product was analyzed with both time-resolved laser-induced fluorescence spectroscopy (TRLFS) and infrared spectroscopy. Both techniques identified the reaction product on DU as a uranium(VI) phosphate phase, but only TRLFS provided its unequivocal identification as meta-autunite based on the positions of the fluorescence emission maxima at 487.8, 502.0, 523.6, 547.0, 572.1, and 600.6 nm and fluorescence lifetimes of 410 +/- 15 and 3300 +/- 310 ns. These results highlight the enhanced performance and sensitivity of the TRLFS technique for mineralogical characterization of thin surface films. Furthermore, they demonstrate that the dissolution of uranium from DU projectiles under the conditions described here is limited by the development and solubility of a meta-autunite secondary phase. The findings have helped clarify the interactions of DU ammunition with phosphate-rich soil-water.
Assuntos
Minerais/química , Urânio/química , Fosfatos de Cálcio/química , Humanos , Lasers , Poluentes Radioativos do Solo/química , Espectrometria de Fluorescência/métodos , Água/químicaRESUMO
Fluorescent uranium(V) and uranium(VI) particles were observed for the first time in vivo by a combined laser fluorescence spectroscopy and confocal laser scanning microscopy approach in a living multispecies biofilm grown on biotite plates. These particles ranged between 1 and 7 um in width and up to 20 microm in length and were located at the bottom and at the edges of biofilms colonies. Analysis of amplified 16S rRNA fragments and fluorescence in situ hybridization were used to characterize the biofilm communities. Laser fluorescence spectroscopy was used to identify these particles. The particles showed either a characteristic fluorescence spectrum in the wavelength range of 415-475 nm, indicative for uranium(V), or in the range of 480-560 nm, which is typical for uranium(VI). Particles of uranium(V) as well as uranium(VI) were simultaneously observed in the biofilms. These uranium particles were attributed for uranium(VI) to biologically mediated precipitation and for uranium(V) to redox processes taking place within the biofilm. The detection of uranium(V) in a multispecies biofilm was interpreted as a short-lived intermediate of the uranium(VI) to uranium(IV) redox reaction. Its presence clearly documents that the uranium(VI) reduction is not a two electron step but that only one electron is involved.
Assuntos
Biofilmes , Microscopia Confocal/métodos , Espectrometria de Fluorescência/métodos , Urânio , Bactérias/genética , Bactérias/crescimento & desenvolvimento , Hibridização in Situ Fluorescente , Tamanho da Partícula , RNA Ribossômico 16S/genéticaRESUMO
Time-resolved laser-induced fluorescence spectroscopy (TRLFS) and high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) were applied to investigate the species of uranyl(VI) adsorbed onto muscovite platelets and muscovite suspensions (grain size: 63-200 microm). TRLFS provided evidence for the presence of two adsorbed uranium(VI) surface species on edge-surfaces of muscovite. The two species showed different positions of the fluorescence emission bands and different fluorescence lifetimes indicating a different coordination environment for the two species. HAADF-STEM revealed that nanoclusters of an amorphous uranium phase were attached to the edge-surfaces of muscovite powder during batch sorption experiments. These U-nanoclusters were not observed on {00/} cleavage planes of the muscovite. The surface species with the shorter fluorescence lifetimes are interpreted as truly adsorbed bidentate surface complexes, in which the U(VI) binds to aluminol groups of edge-surfaces. The surface species with the longer fluorescence lifetimes are interpreted to be an amorphous U(VI) condensate or nanosized clusters of polynuclear uranyl(VI) surface species with a particle diameter of 1 to 2 nm. Depending on the size of these clusters the fluorescence lifetimes vary; i.e., the larger the nanosized clusters, the longer is the fluorescence lifetime.
Assuntos
Silicatos de Alumínio/química , Microscopia Eletrônica de Transmissão/métodos , Minerais/química , Espectrometria de Fluorescência/métodos , Urânio/análise , Adsorção , Galvanoplastia/métodos , Lasers , Nanopartículas Metálicas/análise , Nanotecnologia/métodos , Poluentes Radioativos/análise , Compostos de Urânio/análiseRESUMO
The mechanisms of the uranium(VI) sorption on schwertmannite and goethite in acid sulfate-rich solutions were studied by Extended X-ray Absorption Fine Structure (EXAFS) spectroscopy. The samples were prepared under N2 atmosphere and initial uranium(VI) concentrations of 1 x 10(-5) (pH 6.5) to 5 x 10(-5) M (pH 4.2). The ionic strength was adjusted using 0.01 M Na2SO4 or 0.01 M NaClO4, respectively. The EXAFS structural parameters for uranium(VI) sorbed on goethite in sulfate-rich, acid and near-neutral solutions indicate that uranium(VI) forms an inner-sphere, mononuclear, bidentate surface complex. This complex is characterized by a uranium-ferric-iron distance of approximately 3.45 A. Uranium(VI) sorbed onto schwertmannite in acid and sulfate-rich solution is coordinated to one or two sulfate molecules with a uranium-sulfur distance of 3.67 A. The EXAFS results indicate formation of binuclear, bidentate surface complexes and partly of mononuclear, monodentate surface complexes coordinated to the structural sulfate of schwertmannite. The formation of ternary uranium(VI)-sulfate surface complexes could not be excluded because of the uncertainty in assigning the sulfate either to the bulk structure or to adsorption reactions. The uranium(VI) adsorption onto schwertmannite in perchlorate solution occurs predominantly as a mononuclear, bidentate complexation with ferric iron due to the release of sulfate from the substrate.
Assuntos
Compostos Férricos/química , Sulfatos/química , Urânio/química , Poluentes Radioativos da Água/análise , Adsorção , Monitoramento AmbientalRESUMO
The uranium carbonate andersonite Na2Ca[UO2(CO3)3] x 6H2O was synthesized and identified with classical analytical and spectroscopic methods. The classical methods applied were powder X-ray diffraction (XRD), nitric acid digestion, and scanning electron microcopy combined with energy-dispersive spectroscopy (SEM/EDS). To characterize andersonite spectroscopically, time-resolved laser-induced fluorescence spectroscopy (TRLFS), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FT-IR) were used. Natural and synthetic andersonite samples were characterized with the nondestructive TRLFS by six fluorescence emission bands at 470.6, 486.1, 505.4, 526.7, 549.6, and 573.9 nm. In addition, andersonite was characterized by FT-IR measurements by the appearance of the asymmetric stretching vibration of the uranyl cation [v3(UO2(2+))] at 902 cm(-1) with a shoulder at 913 cm(-1). XPS measurements verified the composition of the synthetic andersonite sample. The measured intensity ratios of the XPS lines agree with the stoichiometry of Na2Ca[UO2(CO3)3] x 6H2O. The XPS features of the inner valence molecular orbitals are characteristic of the [UO2(CO3)3]4- structural moiety. These spectroscopic methods can be used to identify in a fingerprinting procedure secondary U(VI) phases in mixtures with other phases or as thin coatings on mineral and rock surfaces.