PSB33 protein sustains photosystem II in plant chloroplasts under UV-A light.

Plants can quickly and dynamically respond to spectral and intensity variations of the incident light. These responses include activation of developmental processes, morphological changes, and photosynthetic acclimation that ensure optimal energy conversion and minimal photoinhibition. Plant adaptation and acclimation to environmental changes have been extensively studied, but many details surrounding these processes remain elusive. The photosystem II (PSII)-associated protein PSB33 plays a fundamental role in sustaining PSII as well as in the regulation of the light antenna in fluctuating light. We investigated how PSB33 knock-out Arabidopsis plants perform under different light qualities. psb33 plants displayed a reduction of 88% of total fresh weight compared to wild type plants when cultivated at the boundary of UV-A and blue light. The sensitivity towards UV-A light was associated with a lower abundance of PSII proteins, which reduces psb33 plants' capacity for photosynthesis. The UV-A phenotype was found to be linked to altered phytohormone status and changed thylakoid ultrastructure. Our results collectively show that PSB33 is involved in a UV-A light-mediated mechanism to maintain a functional PSII pool in the chloroplast.

Effects of achromatic and chromatic lights on pupillary response, endocrinology, activity, and milk production in dairy cows.

Artificial light can be used as a management tool to increase milk yield in dairy production. However, little is known about how cows respond to the spectral composition of light. The aim of this study was to investigate how dairy cows respond to artificial achromatic and chromatic lights. A tie-stall barn equipped with light-emitting diode (LED) light fixtures was used to create the controlled experimental light environments. Two experiments were conducted, both using dairy cows of Swedish Red and light mixtures with red, blue or white light. In experiment I, the response to light of increasing intensity on pupil size was evaluated in five pregnant non-lactating cows. In experiment II 16h of achromatic and chromatic daylight in combination with dim, achromatic night light, was tested on pregnant lactating cows during five weeks to observe long term effects on milk production, activity and circadian rhythms. Particular focus was given to possible carry over effects of blue light during the day on activity at night since this has been demonstrated in humans. Increasing intensity of white and blue light affected pupil size (P<0.001), but there was no effect on pupil size with increased intensity of red light. Milk yield was maintained throughout experiment II, and plasma melatonin was higher during dim night light than in daylight for all treatments (P<0.001). In conclusion, our results show that LED fixtures emitting red light driving the ipRGCs indirectly via ML-cones, blue light stimulating both S-cones and ipRGCs directly and a mixture of wavelengths (white light) exert similar effects on milk yield and activity in tied-up dairy cows. This suggests that the spectral composition of LED lighting in a barn is secondary to duration and intensity.

Reaching Natural Growth: The Significance of Light and Temperature Fluctuations in Plant Performance in Indoor Growth Facilities.

Recommendations for near-natural plant growth under indoor conditions have been described without considering environmental fluctuations, which might have important consequences for researchers and plant producers when comparing results from indoor facilities with natural ecosystems or production. Previous authors proposed that differences in temperature, light quantity, and the lack of their variation are sources of deviations between indoor and outdoor experiments. Here, we investigated the effect of fluctuating light, temperature, and humidity in an indoor environment on plant performance. Seven plant species from different functional plant types were grown outdoors during summer and spring. The same species were then grown in indoor growth chambers under different scenarios of climate complexity in terms of fluctuations of temperature, air humidity, and light: 1) fixed night and day conditions, 2) daily sinusoidal changes, and 3) variable conditions tracking the climate records from the field trials. In each scenario, the average of the environmental variables was the same as in the respective field trial. Productivity-, gas exchange-, and leaf pigment-traits were measured in all plants at the end of the experiments. The plant trait responses were highly dependent on species and treatment, but general trends were observed. The variable condition yielded lower biomass compared to the fixed and sinusoidal conditions, together with a higher specific leaf area and increased chlorophyll concentrations. A principal component analysis (PCA) across all plant traits in response to climatic conditions suggested that at least a sinusoidal fluctuation is recommended for a more natural-like plant performance in indoor growth facilities. However, prevailing significant differences for several traits between field- and indoor-grown plants even under variable climates indicate that additional factors other than those controllable in standard phytotrons (e.g., wind speed and direction, leaf and soil temperature) can still significantly bias plant performance in indoor facilities.

Reaching Natural Growth: Light Quality Effects on Plant Performance in Indoor Growth Facilities.

To transfer experimental findings in plant research to natural ecosystems it is imperative to reach near to natural-like plant performance. Previous studies propose differences in temperature and light quantity as main sources of deviations between indoor and outdoor plant growth. With increasing implementation of light emitting diodes (LED) in plant growth facilities, light quality is yet another factor that can be optimised to prevent unnatural plant performance. We investigated the effects of different wavelength combinations in phytotrons (i.e., indoor growth chambers) on plant growth and physiology in seven different plant species from different plant functional types (herbs, grasses and trees). The results from these experiments were compared against a previous field trial with the same set of species. While different proportions of blue (B) and red (R) light were applied in the phytotrons, the mean environmental conditions (photoperiod, total radiation, red to far red ratio and day/night temperature and air humidity) from the field trial were used in the phytotrons in order to assess which wavelength combinations result in the most natural-like plant performance. Different plant traits and physiological parameters, including biomass productivity, specific leaf area (SLA), leaf pigmentation, photosynthesis under a standardised light, and the respective growing light and chlorophyll fluorescence, were measured at the end of each treatment. The exposure to different B percentages induced species-specific dose response reactions for most of the analysed parameters. Compared with intermediate B light treatments (25 and/or 35% B light), extreme R or B light enriched treatments (6% and 62% of B respectively) significantly affected the height, biomass, biomass allocation, chlorophyll content, and photosynthesis parameters, differently among species. Principal component analyses (PCA) confirmed that 6% and 62% B light quality combinations induce more extreme plant performance in most cases, indicating that light quality needs to be adjusted to mitigate unnatural plant responses under indoor conditions.