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1.
Mol Ecol ; 31(7): 2124-2139, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35106871

RESUMO

Present-day ecology and population structure are the legacies of past climate and habitat perturbations, and this is particularly true for species that are widely distributed at high latitudes. The red knot, Calidris canutus, is an arctic-breeding, long-distance migratory shorebird with six recognized subspecies defined by differences in morphology, migration behavior, and annual cycle phenology, in a global distribution thought to have arisen just since the last glacial maximum (LGM). We used nextRAD sequencing of 10,881 single-nucleotide polymorphisms (SNPs) to assess the neutral genetic structure and phylogeographic history of 172 red knots representing all known global breeding populations. Using population genetics approaches, including model-based scenario-testing in an approximate Bayesian computation (ABC) framework, we infer that red knots derive from two main lineages that diverged ca. 34,000 years ago, and thus most probably persisted at the LGM in both Palearctic and Nearctic refugia, followed by at least two instances of secondary contact and admixture. Within two Beringian subspecies (C. c. roselaari and rogersi), we detected previously unknown genetic structure among sub-populations sharing a migratory flyway, reflecting additional complexity in the phylogeographic history of the region. Conversely, we found very weak genetic differentiation between two Nearctic populations (rufa and islandica) with clearly divergent migratory phenotypes and little or no apparent contact throughout the annual cycle. Together, these results suggest that relative gene flow among migratory populations reflects a complex interplay of historical, geographical, and ecological factors.


Assuntos
Charadriiformes , Refúgio de Vida Selvagem , Animais , Teorema de Bayes , Variação Genética , Genética Populacional , Filogeografia
2.
Syst Biol ; 68(6): 937-955, 2019 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-31135914

RESUMO

Palaeognathae represent one of the two basal lineages in modern birds, and comprise the volant (flighted) tinamous and the flightless ratites. Resolving palaeognath phylogenetic relationships has historically proved difficult, and short internal branches separating major palaeognath lineages in previous molecular phylogenies suggest that extensive incomplete lineage sorting (ILS) might have accompanied a rapid ancient divergence. Here, we investigate palaeognath relationships using genome-wide data sets of three types of noncoding nuclear markers, together totaling 20,850 loci and over 41 million base pairs of aligned sequence data. We recover a fully resolved topology placing rheas as the sister to kiwi and emu + cassowary that is congruent across marker types for two species tree methods (MP-EST and ASTRAL-II). This topology is corroborated by patterns of insertions for 4274 CR1 retroelements identified from multispecies whole-genome screening, and is robustly supported by phylogenomic subsampling analyses, with MP-EST demonstrating particularly consistent performance across subsampling replicates as compared to ASTRAL. In contrast, analyses of concatenated data supermatrices recover rheas as the sister to all other nonostrich palaeognaths, an alternative that lacks retroelement support and shows inconsistent behavior under subsampling approaches. While statistically supporting the species tree topology, conflicting patterns of retroelement insertions also occur and imply high amounts of ILS across short successive internal branches, consistent with observed patterns of gene tree heterogeneity. Coalescent simulations and topology tests indicate that the majority of observed topological incongruence among gene trees is consistent with coalescent variation rather than arising from gene tree estimation error alone, and estimated branch lengths for short successive internodes in the inferred species tree fall within the theoretical range encompassing the anomaly zone. Distributions of empirical gene trees confirm that the most common gene tree topology for each marker type differs from the species tree, signifying the existence of an empirical anomaly zone in palaeognaths.


Assuntos
Genoma/genética , Paleógnatas/classificação , Paleógnatas/genética , Filogenia , Animais , Genômica
3.
Proc Natl Acad Sci U S A ; 113(38): E5580-7, 2016 09 20.
Artigo em Inglês | MEDLINE | ID: mdl-27573837

RESUMO

Molecular dating largely overturned the paradigm that global cooling during recent Pleistocene glacial cycles resulted in a burst of species diversification although some evidence exists that speciation was commonly promoted in habitats near the expanding and retracting ice sheets. Here, we used a genome-wide dataset of more than half a million base pairs of DNA to test for a glacially induced burst of diversification in kiwi, an avian family distributed within several hundred kilometers of the expanding and retracting glaciers of the Southern Alps of New Zealand. By sampling across the geographic range of the five kiwi species, we discovered many cryptic lineages, bringing the total number of kiwi taxa that currently exist to 11 and the number that existed just before human arrival to 16 or 17. We found that 80% of kiwi diversification events date to the major glacial advances of the Middle and Late Pleistocene. During this period, New Zealand was repeatedly fragmented by glaciers into a series of refugia, with the tiny geographic ranges of many kiwi lineages currently distributed in areas adjacent to these refugia. Estimates of effective population size through time show a dramatic bottleneck during the last glacial cycle in all but one kiwi lineage, as expected if kiwi were isolated in glacially induced refugia. Our results support a fivefold increase in diversification rates during key glacial periods, comparable with levels observed in classic adaptive radiations, and confirm that at least some lineages distributed near glaciated regions underwent rapid ice age diversification.


Assuntos
DNA Mitocondrial/genética , Evolução Molecular , Especiação Genética , Paleógnatas/genética , Animais , Ecossistema , Humanos , Camada de Gelo , Nova Zelândia , Filogenia , Análise de Sequência de DNA
4.
Syst Biol ; 66(6): 1028-1044, 2017 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-28637293

RESUMO

Noncoding markers have a particular appeal as tools for phylogenomic analysis because, at least in vertebrates, they appear less subject to strong variation in GC content among lineages. Thus far, ultraconserved elements (UCEs) and introns have been the most widely used noncoding markers. Here we analyze and study the evolutionary properties of a new type of noncoding marker, conserved nonexonic elements (CNEEs), which consists of noncoding elements that are estimated to evolve slower than the neutral rate across a set of species. Although they often include UCEs, CNEEs are distinct from UCEs because they are not ultraconserved, and, most importantly, the core region alone is analyzed, rather than both the core and its flanking regions. Using a data set of 16 birds plus an alligator outgroup, and ∼3600-∼3800 loci per marker type, we found that although CNEEs were less variable than bioinformatically derived UCEs or introns and in some cases exhibited a slower approach to branch resolution as determined by phylogenomic subsampling, the quality of CNEE alignments was superior to those of the other markers, with fewer gaps and missing species. Phylogenetic resolution using coalescent approaches was comparable among the three marker types, with most nodes being fully and congruently resolved. Comparison of phylogenetic results across the three marker types indicated that one branch, the sister group to the passerine + falcon clade, was resolved differently and with moderate (>70%) bootstrap support between CNEEs and UCEs or introns. Overall, CNEEs appear to be promising as phylogenomic markers, yielding phylogenetic resolution as high as for UCEs and introns but with fewer gaps, less ambiguity in alignments and with patterns of nucleotide substitution more consistent with the assumptions of commonly used methods of phylogenetic analysis.


Assuntos
Classificação/métodos , Sequência Conservada/genética , Marcadores Genéticos , Filogenia , Jacarés e Crocodilos/classificação , Jacarés e Crocodilos/genética , Animais , Aves/classificação , Aves/genética , Genoma/genética , Íntrons/genética
5.
Mol Biol Evol ; 32(1): 23-8, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25415961

RESUMO

The evolution of avian feathers has recently been illuminated by fossils and the identification of genes involved in feather patterning and morphogenesis. However, molecular studies have focused mainly on protein-coding genes. Using comparative genomics and more than 600,000 conserved regulatory elements, we show that patterns of genome evolution in the vicinity of feather genes are consistent with a major role for regulatory innovation in the evolution of feathers. Rates of innovation at feather regulatory elements exhibit an extended period of innovation with peaks in the ancestors of amniotes and archosaurs. We estimate that 86% of such regulatory elements and 100% of the nonkeratin feather gene set were present prior to the origin of Dinosauria. On the branch leading to modern birds, we detect a strong signal of regulatory innovation near insulin-like growth factor binding protein (IGFBP) 2 and IGFBP5, which have roles in body size reduction, and may represent a genomic signature for the miniaturization of dinosaurian body size preceding the origin of flight.


Assuntos
Aves/genética , Dinossauros/anatomia & histologia , Plumas/crescimento & desenvolvimento , Genômica/métodos , Elementos Reguladores de Transcrição , Animais , Evolução Biológica , Aves/anatomia & histologia , Tamanho Corporal , Dinossauros/genética , Dinossauros/crescimento & desenvolvimento , Evolução Molecular , Plumas/metabolismo , Proteína 2 de Ligação a Fator de Crescimento Semelhante à Insulina/genética , Proteína 5 de Ligação a Fator de Crescimento Semelhante à Insulina/genética , Queratinas/genética , Taxa de Mutação , Filogenia
6.
Mol Biol Evol ; 31(7): 1686-96, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24825849

RESUMO

One of the most startling discoveries in avian molecular phylogenetics is that the volant tinamous are embedded in the flightless ratites, but this topology remains controversial because recent morphological phylogenies place tinamous as the closest relative of a monophyletic ratite clade. Here, we integrate new phylogenomic sequences from 1,448 nuclear DNA loci totaling almost 1 million bp from the extinct little bush moa, Chilean tinamou, and emu with available sequences from ostrich, elegant crested tinamou, four neognaths, and the green anole. Phylogenetic analysis using standard homogeneous models and heterogeneous models robust to common topological artifacts recovered compelling support for ratite paraphyly with the little bush moa closest to tinamous within ratites. Ratite paraphyly was further corroborated by eight independent CR1 retroposon insertions. Analysis of morphological characters reinterpreted on a 27-gene paleognath topology indicates that many characters are convergent in the ratites, probably as the result of adaptation to a cursorial life style.


Assuntos
Paleógnatas/classificação , Paleógnatas/genética , Adaptação Fisiológica , Animais , Teorema de Bayes , Evolução Molecular , Genoma , Funções Verossimilhança , Modelos Genéticos , Paleógnatas/anatomia & histologia , Paleógnatas/fisiologia , Filogenia , Análise de Sequência de DNA
7.
Sci Adv ; 10(21): eadj6823, 2024 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-38781323

RESUMO

We present a draft genome of the little bush moa (Anomalopteryx didiformis)-one of approximately nine species of extinct flightless birds from Aotearoa, New Zealand-using ancient DNA recovered from a fossil bone from the South Island. We recover a complete mitochondrial genome at 249.9× depth of coverage and almost 900 megabases of a male moa nuclear genome at ~4 to 5× coverage, with sequence contiguity sufficient to identify more than 85% of avian universal single-copy orthologs. We describe a diverse landscape of transposable elements and satellite repeats, estimate a long-term effective population size of ~240,000, identify a diverse suite of olfactory receptor genes and an opsin repertoire with sensitivity in the ultraviolet range, show that the wingless moa phenotype is likely not attributable to gene loss or pseudogenization, and identify potential function-altering coding sequence variants in moa that could be synthesized for future functional assays. This genomic resource should support further studies of avian evolution and morphological divergence.


Assuntos
Aves , Extinção Biológica , Genoma , Animais , Aves/genética , Núcleo Celular/genética , Filogenia , Fósseis , Genoma Mitocondrial , Voo Animal , Nova Zelândia , Masculino , Elementos de DNA Transponíveis/genética , Genômica/métodos
8.
Immunogenetics ; 65(3): 211-25, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23239370

RESUMO

The major histocompatibility complex (MHC) includes highly polymorphic gene families encoding proteins crucial to the vertebrate acquired immune system. Classical MHC class I (MHCI) genes code for molecules expressed on the surfaces of most nucleated cells and are associated with defense against intracellular pathogens, such as viruses. These genes have been studied in a few wild bird species, but have not been studied in long-distance migrating shorebirds. Red Knots Calidris canutus are medium-sized, monogamous sandpipers with migratory routes that span the globe. Understanding how such long-distance migrants protect themselves from disease has gained new relevance since the emergence of avian-borne diseases, including intracellular pathogens recognized by MHCI molecules, such as avian influenza. In this study, we characterized MHCI genes in knots and found 36 alleles in eight individuals and evidence for six putatively functional and expressed MHCI genes in a single bird. We also found evidence for recombination and for positive selection at putative peptide binding sites in exons 2 and 3. These results suggest surprisingly high MHC diversity in knots, given their demographic history. This may be a result of selection from diverse pathogens encountered by shorebirds throughout their annual migrations.


Assuntos
Charadriiformes/genética , DNA Intergênico/genética , Genes MHC Classe I , Recombinação Genética , Sequência de Aminoácidos , Migração Animal , Animais , Charadriiformes/imunologia , DNA Complementar/genética , Ecossistema , Éxons/genética , Variação Genética , Íntrons/genética , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase , Polimorfismo Genético , RNA Mensageiro/sangue , RNA Mensageiro/genética , Seleção Genética , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Especificidade da Espécie , Transcrição Gênica
9.
Proc Biol Sci ; 279(1747): 4617-25, 2012 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-22977150

RESUMO

The origin and timing of the diversification of modern birds remains controversial, primarily because phylogenetic relationships are incompletely resolved and uncertainty persists in molecular estimates of lineage ages. Here, we present a species tree for the major palaeognath lineages using 27 nuclear genes and 27 archaic retroposon insertions. We show that rheas are sister to the kiwis, emu and cassowaries, and confirm ratite paraphyly because tinamous are sister to moas. Divergence dating using 10 genes with broader taxon sampling, including emu, cassowary, ostrich, five kiwis, two rheas, three tinamous, three extinct moas and 15 neognath lineages, suggests that three vicariant events and possibly two dispersals are required to explain their historical biogeography. The age of crown group birds was estimated at 131 Ma (95% highest posterior density 122-138 Ma), similar to previous molecular estimates. Problems associated with gene tree discordance and incomplete lineage sorting in birds will require much larger gene sets to increase species tree accuracy and improve error in divergence times. The relatively rapid branching within neoaves pre-dates the extinction of dinosaurs, suggesting that the genesis of the radiation within this diverse clade of birds was not in response to the Cretaceous-Paleogene extinction event.


Assuntos
Proteínas Aviárias/genética , Aves/genética , Filogenia , Retroelementos/genética , Animais , Biodiversidade , Fósseis , Especiação Genética , Filogeografia , Análise de Sequência de DNA
10.
Mol Phylogenet Evol ; 65(2): 631-41, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22842291

RESUMO

Molecular phylogenies of Charadriiformes based on mtDNA genes and one to three nuclear loci do not support the traditional placement of Pluvialis in the plovers (Charadriidae), assigning it instead to oystercatchers, stilts, and avocets (Haematopodidae and Recurvirostridae). To investigate this hypothesis of plover paraphyly, the relationships among Pluvialis and closely related families were revisited by sequencing two individuals of all taxa except Peltohyas for eight independent single copy nuclear protein-coding loci selected for their informativeness at this phylogenetic depth. The species tree estimated jointly with the gene trees in the coalescent programme (*)BEAST strongly supported plover monophyly, as did Bayesian analysis of the concatenated matrix. The data sets that supported plover paraphyly in Baker et al. (2007) and Fain and Houde (2007) reflect two to four independent gene histories, and thus discordance with the plover monophyly species tree might have arisen by chance through stochastic mutational variance. For the plovers we conclude there is no conclusive evidence of coalescent variance from ancient incomplete lineage sorting across the interior branch leading to Pluvialis in the species tree. Rather, earlier studies seem have been misled by faster evolving mtDNA genes with high mutational variance, and a few nuclear genes that had low resolving power at the Pluvialis sister group level. These findings are of general relevance in avian phylogenetics, as they show that careful attention needs to be paid to the number and the phylogenetic informativeness of genes required to obtain accurate estimates of the species tree, especially where there is mutational heterogeneity in gene trees.


Assuntos
Núcleo Celular/genética , Charadriiformes/classificação , Evolução Molecular , Filogenia , Animais , Teorema de Bayes , Charadriiformes/genética , DNA Mitocondrial/genética , Modelos Genéticos , Mutação , Alinhamento de Sequência , Análise de Sequência de DNA
11.
Immunogenetics ; 63(6): 377-94, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21327606

RESUMO

A major challenge facing studies of major histocompatibility complex (MHC) evolution in birds is the difficulty in genotyping alleles at individual loci, and the consequent inability to investigate sequence variation and selection pressures for each gene. In this study, four MHC class I loci were isolated from the red-billed gull (Larus scopulinus), representing both the first characterized MHCI genes within Charadriiformes (shorebirds, gulls, and allies) and the first full-length MHCI sequences described outside Galloanserae (gamebirds + waterfowl). Complete multilocus genotypes were obtained for 470 individuals using a combination of reference-strand conformation analysis and direct sequencing of gene-specific amplification products, and variation of peptide-binding region (PBR) exons was surveyed for all loci. Each gene is transcribed and has conserved sequence features characteristic of antigen-presenting MHCI molecules. However, higher allelic variation, a more even allele frequency distribution, and evidence of positive selection acting on a larger number of PBR residues suggest that only one locus (Lasc-UAA) functions as a major classical MHCI gene. Lasc-UBA, with more limited variation and PBR motifs that encompass a subset of Lasc-UAA diversity, was assigned a putative minor classical function, whereas the divergent and largely invariant binding-groove motifs of Lasc-UCA and -UDA are suggestive of nonclassical loci with specialized ligand-binding roles.


Assuntos
Charadriiformes/genética , Charadriiformes/imunologia , Genes MHC Classe I , Alelos , Sequência de Aminoácidos , Animais , Proteínas Aviárias/química , Proteínas Aviárias/genética , Proteínas Aviárias/imunologia , Sequência de Bases , Sítios de Ligação/genética , Sequência Conservada , Primers do DNA/genética , DNA Complementar/genética , Evolução Molecular , Éxons , Variação Genética , Antígenos de Histocompatibilidade Classe I/química , Antígenos de Histocompatibilidade Classe I/genética , Complexo Principal de Histocompatibilidade , Locos Secundários de Histocompatibilidade , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase , Ligação Proteica , Estrutura Terciária de Proteína , Seleção Genética , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido Nucleico
12.
BMC Evol Biol ; 10: 226, 2010 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-20653954

RESUMO

BACKGROUND: Water Rails (Rallus aquaticus) inhabit fragmented freshwater wetlands across their Palearctic distribution. Disjunct populations are now thought to be morphologically similar over their vast geographic range, though four subspecies had been recognized previously. The fossil record suggests that Water Rails (R. aquaticus) were already spread across the Palearctic by the Pleistocene approximately 2 million years ago, and the oldest fossil remains thought to be closely related to the common ancestor of water rails date from the Pliocene. RESULTS: To investigate population structure in Water Rails at the genetic level we sequenced three independent loci: 686 base pairs (bp) of the mitochondrial DNA COI barcode; 618 bp of the intron ADH5; and 746 bp of the exon PTPN12. Phylogeographic analysis revealed that Water Rails breeding in eastern Asia (R. a. indicus, also known as the Brown-cheeked Rail) are strongly differentiated from the Water Rails in Western and Middle Asia and Europe (R. a. aquaticus and R. a. korejewi). The Kimura 3-parameter plus Gamma COI genetic distance between these two geographic groups was > 3%, and they differed by 18 diagnostic substitutions commensurate with differences between recently diverged sister species of birds. In spite of the low number of variable sites, the two nuclear loci supported this split. We estimated the split of the Brown-cheeked Rail and the Water Rail to have occurred approximately 534,000 years ago (95% CI 275,000-990,000 years ago). Fragmentation of the widespread ancestral population and eventual speciation of water rails is likely attributable to vicariance by a barrier formed by glacial cycles, continuous uplift of the Tibetan Plateau and increased sedimentation in deserts in southern Asia that originated in the Miocene. CONCLUSIONS: Water Rails from East Asia were genetically differentiated from the ones breeding in Europe and Western to Middle Asia. Most of the genetic signal was from mitochondrial COI, and was corroborated by polymorphic sites in the two nuclear loci we employed. The split between these two lineages was estimated to occur in the Middle Pleistocene, when populations were isolated in disjunct wetlands with little or no gene flow. Independent evidence from differences in morphology and vocalizations in concert with genetic differentiation and a long history of isolation support recognition of the Brown-cheeked Rail breeding in East Asia as a separate species, R. indicus. The use of several independent loci is invaluable in inferring species trees from gene trees and in recognizing species limits.


Assuntos
Aves/classificação , Aves/genética , Evolução Molecular , Filogenia , Animais , Ásia , Composição de Bases , Teorema de Bayes , DNA Mitocondrial/genética , Europa (Continente) , Fluxo Gênico , Variação Genética , Genética Populacional , Geografia , Haplótipos , Modelos Genéticos , Polimorfismo de Nucleotídeo Único , Análise de Sequência de DNA , Especificidade da Espécie
13.
Mol Phylogenet Evol ; 57(1): 411-6, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20601013

RESUMO

Although the mitochondrial genome in birds has highly conserved features, with protein genes similar to mammals and amphibians, several variations in gene order around the hypervariable control region have been found. Here we report a novel gene arrangement around the control region in shorebirds (Charadriiformes). In ruffs Philomachus pugnax, the mitochondrial genome between cytochrome b and 12SrRNA was over 1.5 kb longer than reported for other Charadriiformes and contained a duplication of the control region together with NADH dehydrogenase subunit 6 (ND6) and the adjacent transfer RNAs: tRNA(Pro) and tRNA(Glu). Sequence data from 68 individuals from several stopover and breeding populations show that the duplication is widespread in ruffs. Similar gene re-arrangements have been found independently in unrelated tube-nosed seabirds and spoonbills.


Assuntos
Charadriiformes/genética , Ordem dos Genes , Genoma Mitocondrial , Animais , Sequência de Bases , DNA Mitocondrial/genética , Evolução Molecular , Rearranjo Gênico , Genes Mitocondriais , Dados de Sequência Molecular , Análise de Sequência de DNA
14.
Science ; 364(6435): 74-78, 2019 04 05.
Artigo em Inglês | MEDLINE | ID: mdl-30948549

RESUMO

A core question in evolutionary biology is whether convergent phenotypic evolution is driven by convergent molecular changes in proteins or regulatory regions. We combined phylogenomic, developmental, and epigenomic analysis of 11 new genomes of paleognathous birds, including an extinct moa, to show that convergent evolution of regulatory regions, more so than protein-coding genes, is prevalent among developmental pathways associated with independent losses of flight. A Bayesian analysis of 284,001 conserved noncoding elements, 60,665 of which are corroborated as enhancers by open chromatin states during development, identified 2355 independent accelerations along lineages of flightless paleognaths, with functional consequences for driving gene expression in the developing forelimb. Our results suggest that the genomic landscape associated with morphological convergence in ratites has a substantial shared regulatory component.


Assuntos
Evolução Biológica , Epigênese Genética , Evolução Molecular , Voo Animal , Paleógnatas/anatomia & histologia , Paleógnatas/genética , Animais , Teorema de Bayes , Cromatina/metabolismo , Sequência Conservada , Elementos Facilitadores Genéticos , Epigenômica , Éxons/genética , Extinção Biológica , Membro Anterior/anatomia & histologia , Paleógnatas/fisiologia , Fenótipo , Filogenia
15.
BMC Evol Biol ; 8: 81, 2008 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-18328107

RESUMO

BACKGROUND: DNA barcoding of life using a standardized COI sequence was proposed as a species identification system, and as a method for detecting putative new species. Previous tests in birds showed that individuals can be correctly assigned to species in ~94% of the cases and suggested a threshold of 10x mean intraspecific difference to detect potential new species. However, these tests were criticized because they were based on a single maternally inherited gene rather than multiple nuclear genes, did not compare phylogenetically identified sister species, and thus likely overestimated the efficacy of DNA barcodes in identifying species. RESULTS: To test the efficacy of DNA barcodes we compared ~650 bp of COI in 60 sister-species pairs identified in multigene phylogenies from 10 orders of birds. In all pairs, individuals of each species were monophyletic in a neighbor-joining (NJ) tree, and each species possessed fixed mutational differences distinguishing them from their sister species. Consequently, individuals were correctly assigned to species using a statistical coalescent framework. A coalescent test of taxonomic distinctiveness based on chance occurrence of reciprocal monophyly in two lineages was verified in known sister species, and used to identify recently separated lineages that represent putative species. This approach avoids the use of a universal distance cutoff which is invalidated by variation in times to common ancestry of sister species and in rates of evolution. CONCLUSION: Closely related sister species of birds can be identified reliably by barcodes of fixed diagnostic substitutions in COI sequences, verifying coalescent-based statistical tests of reciprocal monophyly for taxonomic distinctiveness. Contrary to recent criticisms, a single DNA barcode is a rapid way to discover monophyletic lineages within a metapopulation that might represent undiscovered cryptic species, as envisaged in the unified species concept. This identifies a smaller set of lineages that can also be tested independently for species status with multiple nuclear gene approaches and other phenotypic characters.


Assuntos
Biodiversidade , Aves/genética , Biologia Computacional , DNA Mitocondrial/genética , Genes Mitocondriais , Animais , Aves/classificação , Evolução Molecular , Biblioteca Gênica , Filogenia , Análise de Sequência de DNA , Especificidade da Espécie
16.
J Wildl Dis ; 43(4): 794-7, 2007 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-17984283

RESUMO

As part of the shorebird surveillance, Red Knots (Calidris canutus rufa) were sampled in two Patagonian sites in Argentina, Río Grande and San Antonio Oeste, during 2005-2006. Cloacal swabs and serum samples were collected from 156 birds and tested by virus isolation (Newcastle disease virus), polymerase chain reaction (PCR; avian influenza virus and Plasmodium/Hemoproteus), and for antibodies to St. Louis encephalitis virus. All test results were negative.


Assuntos
Doenças das Aves/epidemiologia , Charadriiformes , Helmintíase Animal/epidemiologia , Infecções Protozoárias em Animais/epidemiologia , Viroses/veterinária , Animais , Argentina/epidemiologia , Aves , Charadriiformes/parasitologia , Charadriiformes/virologia , Feminino , Masculino , Vigilância da População/métodos , Prevalência , Viroses/epidemiologia
17.
J Wildl Dis ; 53(3): 437-446, 2017 07.
Artigo em Inglês | MEDLINE | ID: mdl-28318382

RESUMO

Physiologic traits are promising indicators of population health in the face of rapidly changing environments. We obtained values of diverse physiologic parameters for Two-banded Plovers (Charadrius falklandicus) in coastal sites in Patagonia, Argentina, with the objectives of determining the timeline in which these parameters become affected by the stress of capture and handling and of obtaining reference values for future monitoring of these populations. We analyzed packed cell volume, white blood cell profile, heterophil/lymphocyte ratio, bacterial agglutination titer, and total protein, glucose, triglyceride, and cholesterol levels in apparently healthy birds. Glucose, total white blood cell count, lymphocytes, and eosinophil levels showed changes with handling times >60 min after capture. The remaining parameters did not manifest significant alterations in response to capture and handling of up to 232 min (average=105.2, SD=56.7). Therefore, although researchers should attempt to obtain blood samples as soon as possible after capture, inclusion of physiologic parameters in monitoring studies of species not easily sampled in a few minutes, such as Two-banded Plovers and other shorebird species during migration, should not be discouraged. Here we provide a physiologic report for the species that can be considered as reference values during the nonbreeding season at Patagonian coastal sites.


Assuntos
Migração Animal , Charadriiformes/fisiologia , Animais , Argentina , Aves , Hematócrito , Valores de Referência
18.
Science ; 358(6365): 951-954, 2017 11 17.
Artigo em Inglês | MEDLINE | ID: mdl-29146814

RESUMO

The extinct passenger pigeon was once the most abundant bird in North America, and possibly the world. Although theory predicts that large populations will be more genetically diverse, passenger pigeon genetic diversity was surprisingly low. To investigate this disconnect, we analyzed 41 mitochondrial and 4 nuclear genomes from passenger pigeons and 2 genomes from band-tailed pigeons, which are passenger pigeons' closest living relatives. Passenger pigeons' large population size appears to have allowed for faster adaptive evolution and removal of harmful mutations, driving a huge loss in their neutral genetic diversity. These results demonstrate the effect that selection can have on a vertebrate genome and contradict results that suggested that population instability contributed to this species's surprisingly rapid extinction.


Assuntos
Columbidae/genética , Extinção Biológica , Variação Genética , Seleção Genética , Animais , Núcleo Celular/genética , Genes Mitocondriais/genética , Genômica , Mutação , América do Norte , Densidade Demográfica
19.
Proc Biol Sci ; 273(1582): 11-7, 2006 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-16519228

RESUMO

Classic problems in historical biogeography are where did penguins originate, and why are such mobile birds restricted to the Southern Hemisphere? Competing hypotheses posit they arose in tropical-warm temperate waters, species-diverse cool temperate regions, or in Gondwanaland approximately 100 mya when it was further north. To test these hypotheses we constructed a strongly supported phylogeny of extant penguins from 5851 bp of mitochondrial and nuclear DNA. Using Bayesian inference of ancestral areas we show that an Antarctic origin of extant taxa is highly likely, and that more derived taxa occur in lower latitudes. Molecular dating estimated penguins originated about 71 million years ago in Gondwanaland when it was further south and cooler. Moreover, extant taxa are inferred to have originated in the Eocene, coincident with the extinction of the larger-bodied fossil taxa as global climate cooled. We hypothesize that, as Antarctica became ice-encrusted, modern penguins expanded via the circumpolar current to oceanic islands within the Antarctic Convergence, and later to the southern continents. Thus, global cooling has had a major impact on penguin evolution, as it has on vertebrates generally. Penguins only reached cooler tropical waters in the Galapagos about 4 mya, and have not crossed the equatorial thermal barrier.


Assuntos
Migração Animal , Spheniscidae/genética , Animais , Regiões Antárticas , Citocromos b/genética , Genes RAG-1 , Filogenia , RNA/genética , RNA Mitocondrial , RNA Ribossômico/genética , RNA Ribossômico 16S/genética , Spheniscidae/classificação , Spheniscidae/crescimento & desenvolvimento
20.
Proc Biol Sci ; 273(1586): 587-94, 2006 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-16537130

RESUMO

The success of introduced species is frequently explained by their escape from natural enemies in the introduced region. We tested the enemy release hypothesis with respect to two well studied blood parasite genera (Plasmodium and Haemoproteus) in native and six introduced populations of the common myna Acridotheres tristis. Not all comparisons of introduced populations to the native population were consistent with expectations of the enemy release hypothesis. Native populations show greater overall parasite prevalence than introduced populations, but the lower prevalence in introduced populations is driven by low prevalence in two populations on oceanic islands (Fiji and Hawaii). When these are excluded, prevalence does not differ significantly. We found a similar number of parasite lineages in native populations compared to all introduced populations. Although there is some evidence that common mynas may have carried parasite lineages from native to introduced locations, and also that introduced populations may have become infected with novel parasite lineages, it may be difficult to differentiate between parasites that are native and introduced, because malarial parasite lineages often do not show regional or host specificity.


Assuntos
Doenças das Aves/parasitologia , Haemosporida/crescimento & desenvolvimento , Malária Aviária/parasitologia , Plasmodium/crescimento & desenvolvimento , Estorninhos , Animais , Doenças das Aves/epidemiologia , Citocromos b/química , Citocromos b/genética , DNA Mitocondrial/química , DNA Mitocondrial/genética , Complexo IV da Cadeia de Transporte de Elétrons/química , Complexo IV da Cadeia de Transporte de Elétrons/genética , Haemosporida/genética , Índia/epidemiologia , Malária Aviária/epidemiologia , Filogenia , Plasmodium/genética , Reação em Cadeia da Polimerase/veterinária , Prevalência , Análise de Sequência de DNA
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