[Comparison of the mechanism of cardiotonic action of the fraction 3 of MAI-BAO and digoxin on rat isolated heart]. / Comparison du mecanisme de l'action cardiotonique de la fraction 3 de MAÏ-BAO et de la digoxine sur le coeur isole de rat.

UNLABELLED: MAI-BAO (MB) is a beverage obtained by fermentation of sweet Camellia sinensis tea by the Tea-Mushroom treasure. The action of the chromatographic fraction 3 of MAI-BAO (MB-F3) is cardiotonic. AIMS: To compare the mechanism of cardiotonic activity of MB-F3 and digoxin (Dx) in rats. MATERIALS AND METHODS: MB-F3 obtained by gel chromatography on sephadex G-50 and digoxin are used to infuse the isolated rat heart with the device Langendhorff for the record of cardiac contractions. The biochemical mechanism of cardiac effect of MB-F3 and Dx was determined by measuring phosphorus by the method of Sumner. RESULTS: MB-F3 has a significant positive inotropic effect (P<0.05) as Dx (P <0.01) with respective effective Doses 50% 1mg/ml and 10-6 mg/ml. Furthermore, MB-F3 exerts a competitive inhibition on the ATPase-Na+/K+ less than Dx (P<0.05). CONCLUSION: MB-F3 exerted digitalis-like effect less than digoxin on the rat heart.

[Reversible immunosuppression, liver and kidney markers, calcium and phosphorus in the healthy rabbit]. / Immunosuppression reversible, marqueurs du foie, des reins et metabolisme phosphocalcique chez le lapin sain.

AIM: The aim of this study was to determine one or several doses of Methylprednisolone ( MP) who leads a long time of immunosuppression without disrupting phosphor and calcium, liver and kidney markers at the healthy rabbit. MATERIAL AND METHODS: This study was made to fifteen rabbit. Five (5) batches were constituted according to Nacl and Methylpredmisolone administered dose by body weight. Control batch ( Nacl 0,9%); batch I (2,5mg / kg MP); batch II ( 5mg / kg MP); batch III ( 10mg / kg MP) and batch IV ( 15 mg / kg MP). Biochemical parameters were measured by chemical and enzymatic methods. RESULTS: The results of this study showed an immunosuppression during seven days with 10 and 15 mg / kg of MP doses (P < 0.05). The biochemical disturbances were only observed with 15 mg / kg dose where calcium was lowered to day 15 and TGO increased to day 3 according to day 0 (P < 0.05). CONCLUSION: This study showed that the doses which lead a long time of immunosuppression ( 7 days) are 10 and 15 mg / kg of MP, then the dose which does not disrupt the biochemical parameters is 10 mg / kg of MP.