INTEGRAL METHOD FOR ASSESSING THE EFFICIENCY OF DENTAL CARIES PREVENTION.

This is a part of research work of the Therapeutic Dentistry Department of the Poltava State Medical University (PSMU). The name of this research work is "Development of pathogenetic prevention of pathological changes in the oral cavity in patients with internal diseases" (state registration No. 0121U108263).

HERBAL MEDICINES ANTIMICROBIAL EFFECT.

The aim - to study and compare the sensitivity of museum strains of microorganisms to the herbal medicines. Standard strains of S. aureus ATCC 25923, S. epidermidis ATCC 14990, E. faecalis ATCC 29212, E. coli ATCC 25922 and C. albicans ATCC 10231 were used for the microbiological examination. Antimicrobial activity of the herbal medicines was studied by the disk diffusion method and serial dilutions method according to European Committee on Antimicrobial Susceptibility Testing. According to the obtained results E. coli standard strain is maximally sensitive to tymsal. Enterococci were statistically significant high sensitive to panavir only. Museum strain of S.epidermidis was 2.17 more sensitive to tymsal than to chlorhexidine control (p<0.05). Yeast like fungi standard strain was the most sensitive to proteflazid. Growth inhibition zone was showing the sensitiveness of C.albicans to this herbal medicine, the fungicidal activity was statistically significantly higher than chlorhexidine effect in 1.2 times (p<0.05). Analysis of microorganism's sensitivity to the herbal medicines action had showed the best antimicrobial activity of herbal medicines tymsal, panavir and proteflazid compared to traditional drugs.

Surface modification of the laser powder bed-fused Ti-Zr-Nb scaffolds by dynamic chemical etching and Ag nanoparticles decoration.

Metallic lattice scaffolds are designed to mimic the architecture and mechanical properties of bone tissue and their surface compatibility is of primary importance. This study presents a novel surface modification protocol for metallic lattice scaffolds printed from a superelastic Ti-Zr-Nb alloy. This protocol consists of dynamic chemical etching (DCE) followed by silver nanoparticles (AgNP) decoration. DCE, using an 1HF + 3HNO3 + 12H2O23% based solution, was used to remove partially-fused particles from the surfaces of different as-built lattice structures (rhombic dodecahedron, sheet gyroid, and Voronoi polyhedra). Subsequently, an antibacterial coating was synthesized on the surface of the scaffolds by a controlled (20 min at a fixed volume flowrate of 500 mL/min) pumping of the functionalization solutions (NaBH4 (2 mg/mL) and AgNO3 (1 mg/mL)) through the porous structures. Following these treatments, the scaffolds' surfaces were found to be densely populated with Ag nanoparticles and their agglomerates, and manifested an excellent antibacterial effect (Ag ion release rate of 4-8 ppm) suppressing the growth of both E. coli and B. subtilis bacteria up to 99 %. The scaffold extracts showed no cytotoxicity and did not affect cell proliferation, indicating their safety for subsequent use as implants. A cytocompatibility assessment using MG-63 spheroids demonstrated good attachment, spreading, and active migration of cells on the scaffold surface (over 96 % of living cells), confirming their biotolerance. These findings suggest the promise of this surface modification approach for developing superelastic Ti-Zr-Nb scaffolds with superior antibacterial properties and biocompatibility, making them highly suitable for bone implant applications.