Input compensation of dolphin and sea lion auditory brainstem responses using frequency-modulated up-chirps.

Frequency-modulated "chirp" stimuli that offset cochlear dispersion (i.e., input compensation) have shown promise for increasing auditory brainstem response (ABR) amplitudes relative to traditional sound stimuli. To enhance ABR methods with marine mammal species known or suspected to have low ABR signal-to-noise ratios, the present study examined the effects of broadband chirp sweep rate and level on ABR amplitude in bottlenose dolphins and California sea lions. "Optimal" chirps were designed based on previous estimates of cochlear traveling wave speeds (using high-pass subtractive masking methods) in these species. Optimal chirps increased ABR peak amplitudes by compensating for cochlear dispersion; however, chirps with similar (or higher) frequency-modulation rates produced comparable results. The optimal chirps generally increased ABR amplitudes relative to noisebursts as threshold was approached, although this was more obvious when sound pressure level was used to equate stimulus levels (as opposed to total energy). Chirps provided progressively less ABR amplitude gain (relative to noisebursts) as stimulus level increased and produced smaller ABRs at the highest levels tested in dolphins. Although it was previously hypothesized that chirps would provide larger gains in sea lions than dolphins-due to the lower traveling wave speed in the former-no such pattern was observed.

Output compensation of auditory brainstem responses in dolphins and sea lions.

Cochlear dispersion causes increasing delays between neural responses from high-frequency regions in the cochlear base and lower-frequency regions toward the apex. For broadband stimuli, this can lead to neural responses that are out-of-phase, decreasing the amplitude of farfield neural response measurements. In the present study, cochlear traveling-wave speed and effects of dispersion on farfield auditory brainstem responses (ABRs) were investigated by first deriving narrowband ABRs in bottlenose dolphins and California sea lions using the high-pass subtractive masking technique. Derived-band ABRs were then temporally aligned and summed to obtain the "stacked ABR" as a means of compensating for the effects of cochlear dispersion. For derived-band responses between 8 and 32 kHz, cochlear traveling-wave speeds were similar for sea lions and dolphins [∼2-8 octaves (oct)/ms for dolphins; ∼3.5-11 oct/ms for sea lions]; above 32 kHz, traveling-wave speed for dolphins increased up to ∼30 oct/ms. Stacked ABRs were larger than unmasked, broadband ABRs in both species. The amplitude enhancement was smaller in dolphins than in sea lions, and enhancement in both species appears to be less than reported in humans. Results suggest that compensating for cochlear dispersion will provide greater benefit for ABR measurements in species with better low-frequency hearing.

The offset auditory brainstem response in bottlenose dolphins (Tursiops truncatus): Evidence for multiple underlying processes.

The auditory brainstem response (ABR) to stimulus onset has been extensively used to investigate dolphin hearing. The mechanisms underlying this onset response have been thoroughly studied in mammals. In contrast, the ABR evoked by sound offset has received relatively little attention. To build upon previous observations of the dolphin offset ABR, a series of experiments was conducted to (1) determine the cochlear places responsible for response generation and (2) examine differences in response morphologies when using toneburst versus noiseburst stimuli. Measurements were conducted with seven bottlenose dolphins (Tursiops truncatus) using tonebursts and spectrally "pink" broadband noisebursts, with highpass noise used to limit the cochlear regions involved in response generation. Results for normal-hearing and hearing-impaired dolphins suggest that the offset ABR contains contributions from at least two distinct responses. One type of response (across place) might arise from the activation of neural units that are shifted basally relative to stimulus frequency and shares commonalities with the onset ABR. A second type of response (within place) appears to represent a "true" offset response from afferent centers further up the ascending auditory pathway from the auditory nerve, and likely results from synchronous activity beginning at or above the cochlear nucleus.

Auditory brainstem responses during aerial testing with bottlenose dolphins (Tursiops truncatus): Effects of electrode and jawphone locations.

Transmission of sound to dolphins during electrophysiological hearing screening is conducted out of water in certain cases (e.g., strandings). This necessitates that sound be delivered using a contact transducer either pressed against the skin or affixed to the jaw using a suction cup (i.e., "jawphones"). This study examined how bottlenose dolphin (Tursiops truncatus, n = 3) auditory brainstem responses (ABRs) varied with electrode and jawphone location during aerial testing. Stimuli were tone bursts with center frequencies of 28 to 160 kHz. Regression-based thresholds were lowest with the jawphone on the posterior and middle parts of the mandible. Thresholds based on later ABR peaks-recorded using an electrode immediately behind the blowhole-suggested more similarity between the thresholds for the anterior tip of the rostrum and the posterior/middle mandible than those based on earlier monaural waves recorded near the meatus. This was likely a result of a summation of responses from both ears as opposed to a more efficient acoustic pathway to the ear. These patterns were independent of frequency. These findings provide guidance for jawphone and electrode locations when examining dolphin hearing and when interpreting relative acoustic sensitivity of the head in similar testing situations.

Offset auditory brainstem response (ABR) amplitude in bottlenose dolphins.

Although commonly recorded as onset responses, the auditory brainstem response (ABR) can also be elicited at stimulus offset. The offset ABR has not been extensively investigated in marine mammals. Three normal hearing (NH) and three hearing impaired (HI) dolphins were assessed while fully submerged in sea water. Stimulus spectrum, level, rise/fall time (RFT), and plateau duration were manipulated. Onset and offset ABR amplitude were quantified as the rms voltage 1-7 ms following stimulus onset or offset, respectively. For the same stimulus conditions, onset and offset responses were often larger for NH than HI dolphins, and offset responses were typically smaller than onset responses. For the level series, offset response amplitude typically increased with increasing stimulus level, although offset responses were not 3 dB above the noisefloor for 113-kHz tonebursts. Increasing RFT decreased onset and offset response amplitude. For the 40-kHz tonebursts, a RFT of 32 µs produced a large amplitude offset ABR in NH dolphins. Offset responses for 113-kHz tonebursts were 3 dB above the noisefloor at the shortest RFTs. Offset responses were largest for 4 ms duration stimuli (likely due to overlapping onset and offset response analysis windows), but otherwise, offset responses changed little with increasing duration.

Role of the temporal window in dolphin auditory brainstem response onset.

Auditory brainstem responses (ABRs) to linear-enveloped, broadband noisebursts were measured in six bottlenose dolphins to examine relationships between sound onset envelope properties and the ABR peak amplitude. Two stimulus manipulations were utilized: (1) stimulus onset envelope pressure rate-of-change was held constant while plateau pressure and risetime were varied and (2) plateau duration was varied while plateau pressure and risetime were held constant. When the stimulus onset envelope pressure rate-of-change was held constant, ABR amplitudes increased with risetime and were fit well with an exponential growth model. The model best-fit time constants for ABR peaks P1 and N5 were 55 and 64 µs, respectively, meaning ABRs reached 99% of their maximal amplitudes for risetimes of 275-320 µs. When plateau pressure and risetime were constant, ABR amplitudes increased linearly with stimulus sound exposure level up to durations of ∼250 µs. The results highlight the relationship between ABR amplitude and the integral of some quantity related to the stimulus pressure envelope over the first ∼250 µs following stimulus onset-a time interval consistent with prior estimates of the dolphin auditory temporal window, also known as the "critical interval" in hearing.

Signal-to-noise ratio of auditory brainstem responses (ABRs) across click rate in the bottlenose dolphin (Tursiops truncatus).

Although the maximum length sequence (MLS) and iterative randomized stimulation and averaging (I-RSA) methods allow auditory brainstem response (ABR) measurements at high rates, it is not clear if high rates allow ABRs of a given quality to be measured in less time than conventional (CONV) averaging (i.e., fixed interstimulus intervals) at lower rates. In the present study, ABR signal-to-noise ratio (SNR) was examined in six bottlenose dolphins as a function of measurement time and click rate using CONV averaging at rates of 25 and 100 Hz and the MLS/I-RSA approaches at rates from 100 to 1250 Hz. Residual noise in the averaged ABR was estimated using (1) waveform amplitude following the ABR, (2) waveform amplitude after subtracting two subaverage ABRs (i.e., the "±average"), and (3) amplitude variance at a single time point. Results showed that high stimulus rates can be used to obtain dolphin ABRs with a desired SNR in less time than CONV averaging. Optimal SNRs occurred at rates of 500-750 Hz, but were only a few dB higher than that for CONV averaging at 100 Hz. Nonetheless, a 1-dB improvement in SNR could result in a 25% time savings in reaching criterion SNR.

Effects of stimulus cosine onset properties on bottlenose dolphin (Tursiops truncatus) auditory brainstem responses.

Although the auditory brainstem response (ABR) is known to be an onset response, the specific relationship between stimulus onset properties and the resulting ABR is not well understood. In this study, the effects of stimulus onset on dolphin ABR were examined by measuring ABRs in six bottlenose dolphins while systematically manipulating rise time and plateau sound pressure of cosine-enveloped noise bursts. Noise bursts were spectrally "pink" with frequency content from 10 to 160 kHz, rise times from 32 µs to 4 ms, and plateau sound pressure levels from 102 to 138 dB re 1 µPa. Envelope rise time and plateau sound pressure alone were found to be poor predictors for ABR peak amplitudes and latencies. Peak amplitudes were well described by the envelope sound pressure at the end of a 260-µs window; however, best-fits to the data across ABR peaks were obtained when the window start time was allowed to vary. Peak latencies were best described by the maximum value of the second derivative of the pressure envelope. These results are consistent with single-unit and nearfield response data for terrestrial mammals and indicate that stimuli with rise times greater than 260 µs are non-optimal with respect to maximizing ABR amplitudes.

Comparison of maximum length sequence and randomized stimulation and averaging methods on the bottlenose dolphin auditory brainstem response.

The purpose of the present study was to compare auditory brainstem responses (ABRs) using two approaches that allow the use of high stimulation rates, but with different temporal variability in the interstimulus interval: maximum length sequences (MLS) and iterative randomized stimulation and averaging (I-RSA). ABRs were obtained to click stimuli in six bottlenose dolphins (Tursiops truncatus). In experiment 1, click level was held constant and click rate varied from 25 to 1250 Hz. For MLS, interstimulus intervals varied by a factor of 6 at each rate, while for I-RSA the interstimulus intervals varied by ± 0.5 ms regardless of rate. In experiment 2, stimulus rates ranged from 100 to 1000 Hz and click level varied from 105 to 135 dB re: 1 µPa. For experiment 1, MLS and I-RSA showed similar decreases in ABR peak amplitudes and increases in ABR peak latencies and interwave intervals with increasing rate. For experiment 2, there was an increase in peak latency and a decrease in peak amplitude with decreasing click level; however, the effects of click level were reduced at higher rates. The results indicate that the greater jitter for MLS compared to I-RSA does not substantially affect the dolphin ABR.

Effects of noise burst rise time and level on bottlenose dolphin (Tursiops truncatus) auditory brainstem responses.

Although the auditory brainstem response (ABR) is known to be an onset response, specific features of acoustic stimuli that affect the morphology of the ABR are not well understood. In this study, the effects of stimulus onset properties were investigated by measuring ABRs in seven bottlenose dolphins while systematically manipulating stimulus rise time and the amplitude of the sound pressure temporal envelope plateau. Stimuli consisted of spectrally pink (i.e., equal mean-square pressure in proportional frequency bands) noise bursts with linear rise (and fall) envelopes and frequency content from 10 to 160 kHz. Noise burst rise times varied from 32 µs to 4 ms and plateau sound pressure levels varied from 96 to 150 dB re 1 µPa. ABR peak latency was found to be a function of the rate of change of the sound pressure envelope, while ABR peak amplitude was a function of the envelope sound pressure at the end of a fixed integration window. The data support previous single-unit and nearfield response data from terrestrial mammals and a model where the rate of change of envelope sound pressure is integrated across a time window aligned with stimulus onset.

Stimulus bandwidth impact on auditory evoked potential thresholds and estimated upper-frequency limits of hearing in dolphins.

The frequency range of hearing is important for assessing the potential impact of anthropogenic noise on marine mammals. Auditory evoked potentials (AEPs) are commonly used to assess toothed whale hearing, but measurement methods vary across researchers and laboratories. In particular, estimates of the upper-frequency limit of hearing (UFL) can vary due to interactions between the unintended spread of spectral energy to frequencies below the desired test frequency and a sharp decline in hearing sensitivity at frequencies near the UFL. To assess the impact of stimulus bandwidth on UFL measurement, AEP hearing tests were conducted in four bottlenose dolphins (Tursiops truncatus) with normal and impaired hearing ranges. Dolphins were tested at frequencies near the UFL and at a frequency 1/2-octave below the UFL, where hearing sensitivity was better (i.e., threshold was lower). Thresholds were measured using sinusoidal amplitude modulated (SAM) tones and tone-bursts of varying bandwidth. Measured thresholds varied inversely as a function of stimulus bandwidth near the UFL with narrow-band tone-bursts approximating thresholds measured using SAM tones. Bandwidth did not impact measured thresholds where hearing was more sensitive, highlighting how stimulus bandwidth and the rate of decline of hearing sensitivity interact to affect measured threshold near the UFL.

Click reception in the harbor porpoise (Phocoena phocoena): Effects of electrode and contact transducer location on the auditory brainstem response.

Unlike terrestrial mammals that have unambiguous aerial sound transmission pathways via the outer ear and tympanum, sound reception pathways in most odontocetes are not well understood. Recent studies have used auditory brainstem response (ABR) measurements to examine sound reception pathways. This study sought to determine how sound source placements, recording electrode arrangements, and ABR peak analyses affect interpretations of sound reception in the harbor porpoise (Phocoena phocoena). Click stimuli were delivered in air from a contact transducer ("jawphone"). Early ABR peaks (representing auditory nerve responses), and later peaks reflecting higher brainstem activity, were analyzed across jawphone and recording electrode positions. Auditory nerve responses were similar for jawphone placements from the ipsilateral posterior mandible to the tip of the rostrum. Later peaks, however, suggested a possible region of highest sensitivity midway between the posterior mandible and the rostrum tip. These findings are generally similar to previous data for porpoises. In contrast to auditory nerve responses that were largest when recorded near the ipsilateral meatus, later ABR peaks were largest when recorded with a contralateral (opposing) electrode. These results provide information on the processes underlying peaks of the ABR, and inform stimulus delivery and ABR recording parameters in odontocete sound reception studies.

The effects of click rate on the auditory brainstem response of bottlenose dolphins.

Rate manipulations can be used to study adaptation processes in the auditory nerve and brainstem. For this reason, rate effects on the click-evoked auditory brainstem response (ABR) have been investigated in many mammals, including humans. In this study, click-evoked ABRs were obtained in eight bottlenose dolphins (Tursiops truncatus) while varying stimulus rate using both conventional averaging and maximum length sequences (MLSs), which allow disentangling ABRs that overlap in time and thus permit the study of adaptation at high rates. Dolphins varied in age and upper cutoff frequency of hearing. Conventional stimulation rates were 25, 50, and 100 Hz and average MLS rates were approximately 50, 100, 250, 500, 1000, 2500, and 5000 Hz. Click peak-equivalent sound pressure levels for all conditions were 135 dB re 1 µPa. ABRs were observed in all dolphins, at all stimulus rates. With increasing rate, peak latencies increased and peak amplitudes decreased. There was a trend for an increase in the interwave intervals with increasing rate, which appeared more robust for the dolphins with a full range of hearing. For those rates where ABRs were obtained for both conventional and MLS approaches, the latencies of the mean data were in good agreement.

A review of the history, development and application of auditory weighting functions in humans and marine mammals.

This document reviews the history, development, and use of auditory weighting functions for noise impact assessment in humans and marine mammals. Advances from the modern era of electroacoustics, psychophysical studies of loudness, and other related hearing studies are reviewed with respect to the development and application of human auditory weighting functions, particularly A-weighting. The use of auditory weighting functions to assess the effects of environmental noise on humans-such as hearing damage-risk criteria-are presented, as well as lower-level effects such as annoyance and masking. The article also reviews marine mammal auditory weighting functions, the development of which has been fundamentally directed by the objective of predicting and preventing noise-induced hearing loss. Compared to the development of human auditory weighting functions, the development of marine mammal auditory weighting functions have faced additional challenges, including a large number of species that must be considered, a lack of audiometric information on most species, and small sample sizes for nearly all species for which auditory data are available. The review concludes with research recommendations to address data gaps and assumptions underlying marine mammal auditory weighting function design and application.

Bottlenose dolphin (Tursiops truncatus) auditory brainstem responses to frequency-modulated "chirp" stimuli.

Previous studies have demonstrated that increasing-frequency chirp stimuli (up-chirps) can enhance human auditory brainstem response (ABR) amplitudes by compensating for temporal dispersion occurring along the cochlear partition. In this study, ABRs were measured in two bottlenose dolphins (Tursiops truncatus) in response to spectrally white clicks, up-chirps, and decreasing-frequency chirps (down-chirps). Chirp durations varied from 125 to 2000 µs. For all stimuli, frequency bandwidth was constant (10-180 kHz) and peak-equivalent sound pressure levels (peSPLs) were 115, 125, and 135 dB re 1 µPa. Up-chirps with durations less than ∼1000 µs generally increased ABR peak amplitudes compared to clicks with the same peSPL or energy flux spectral density level, while down-chirps with durations from above ∼250 to 500 µs decreased ABR amplitudes relative to clicks. The findings generally mirror those from human studies and suggest that the use of chirp stimuli may be an effective way to enhance broadband ABR amplitudes in larger marine mammals.

Place specificity of the click-evoked auditory brainstem response in the bottlenose dolphin (Tursiops truncatus).

Cochlear place specificity of the auditory brainstem response (ABR) was investigated in five bottlenose dolphins by measuring ABRs to broadband clicks presented simultaneously with masking noise having various high-pass cutoff frequencies. Click and noise stimuli were digitally compensated to account for the transmitting response of the piezoelectric transducers and any multipath propagation effects to achieve "white" or "pink" spectral characteristics. Narrowband evoked responses were derived by sequentially subtracting responses obtained with noise at lower high-pass cutoff frequencies from those obtained with noise having higher cutoff frequencies. The results revealed little contribution to the click-evoked brainstem response from frequency bands below 10 kHz and, in dolphins with full hearing bandwidth, the largest amplitude derived band evoked responses were obtained from the highest frequency bands. Narrowband latencies decreased with increasing frequency and were adequately fit with a power function exhibiting relatively large change in latency with frequency below ∼30 kHz and little change above ∼30 kHz. These data demonstrate that frequency bands below ∼10 kHz do not substantively contribute to the farfield ABR in the bottlenose dolphin when using place-specific approaches such as high-pass subtractive-masking techniques.

The effects of click and masker spectrum on the auditory brainstem response of bottlenose dolphins (Tursiops truncatus).

Two experiments were performed that investigated the effects of (1) click level and (2) continuous broadband noise on the binaural auditory brainstem response (ABR) of normal-hearing and hearing-impaired bottlenose dolphins (Tursiops truncatus). In addition to spectrally uncompensated clicks and noise, stimuli were digitally compensated to achieve "white" spectra (flat spectral density level) or "pink" spectra (spectral density level rolling off at -3 dB/octave). For experiment 1, in all spectral conditions, ABR peak latencies increased and peak amplitudes decreased with decreasing click level, but interwave intervals changed little. Latency-intensity function (LIF) slopes ranged from -3 to -11 µs/dB. The LIF slopes of ABR peaks evoked by uncompensated clicks were steeper in dolphins with hearing loss. Click level was held constant during experiment 2, and the effect of bilaterally delivered broadband masking noise on the ABR was investigated. Clicks and noise were filtered to create a pink click/noise condition and a white click/noise condition. With increasing levels of masking noise, peak latencies increased (although only P1-P4 white reached significance), peak amplitudes decreased, and interpeak intervals increased (although not significantly). These effects are compared to results reported for terrestrial mammals, and implications for auditory health assessment and biosonar function are discussed.

Effects of age on the tuning of the cVEMP and oVEMP.

OBJECTIVES: The purpose of the present investigation was to define for young, middle-aged, and older adults the optimal frequency (cies) to record both the cervical vestibular-evoked myogenic potential (cVEMP) and the ocular vestibular-evoked myogenic potential (oVEMP). Further, this study aimed to describe age-related changes in the tuning of these two vestibular-evoked myogenic potentials. DESIGN: This was a prospective study. Participants were 39 healthy adults (mean age 46.3 ± 15.7 years; range = 22 to 78 years; 15 men) equally divided into 3 age groups of 13 participants each: young adult (18 to 39 years), middle age (40 to 59 years), and old adult (≥60 years). cVEMPs and oVEMPs were recorded using air-conduction tone bursts at stimulus frequencies of 125, 250, 500, 750, 1000, 1500, and 2000 Hz presented at 127 dB pSPL. RESULTS: There was a significant main effect of age group and frequency on the amplitude of both the cVEMP and the oVEMP. Amplitudes were largest for the Young adult group for the cVEMP and for the young adult and Middle age group for the oVEMP. The largest average peak-to-peak amplitude occurred in response to a 750 Hz tone burst for both responses. No significant differences in mean amplitude of the cVEMP or oVEMP were observed for 500, 750, or 1000 Hz stimuli. There was a significant interaction of age group and frequency for the cVEMP, suggesting a loss of tuning for the old adult group. Compared with the young adult group, the tuning of the cVEMP and oVEMP for the older adjults appeared to shift to a higher frequency. CONCLUSION: There is no sharp tuning in the saccule and utricle. Instead, there is a range of best frequencies that may be used to evoke the cVEMP and oVEMP responses. The results of the present investigation also demonstrate that the optimal stimulus frequency to elicit a VEMP may change with age. Accordingly, 500 Hz may not be the ideal frequency to elicit VEMPs for all age groups. For this reason, in cases where the VEMP response is absent at 500 Hz it is recommended that attempts be made to record the VEMP for tone-burst frequencies of 750 or 1000 Hz.

The effect of muscle contraction level on the cervical vestibular evoked myogenic potential (cVEMP): usefulness of amplitude normalization.

BACKGROUND: The cervical vestibular evoked myogenic potential (cVEMP) is a reflexive change in sternocleidomastoid (SCM) muscle contraction activity thought to be mediated by a saccular vestibulo-collic reflex. CVEMP amplitude varies with the state of the afferent (vestibular) limb of the vestibulo-collic reflex pathway, as well as with the level of SCM muscle contraction. It follows that in order for cVEMP amplitude to reflect the status of the afferent portion of the reflex pathway, muscle contraction level must be controlled. Historically, this has been accomplished by volitionally controlling muscle contraction level either with the aid of a biofeedback method, or by an a posteriori method that normalizes cVEMP amplitude by the level of muscle contraction. A posteriori normalization methods make the implicit assumption that mathematical normalization precisely removes the influence of the efferent limb of the vestibulo-collic pathway. With the cVEMP, however, we are violating basic assumptions of signal averaging: specifically, the background noise and the response are not independent. The influence of this signal-averaging violation on our ability to normalize cVEMP amplitude using a posteriori methods is not well understood. PURPOSE: The aims of this investigation were to describe the effect of muscle contraction, as measured by a prestimulus electromyogenic estimate, on cVEMP amplitude and interaural amplitude asymmetry ratio, and to evaluate the benefit of using a commonly advocated a posteriori normalization method on cVEMP amplitude and asymmetry ratio variability. RESEARCH DESIGN: Prospective, repeated-measures design using a convenience sample. STUDY SAMPLE: Ten healthy adult participants between 25 and 61 yr of age. INTERVENTION: cVEMP responses to 500 Hz tone bursts (120 dB pSPL) for three conditions describing maximum, moderate, and minimal muscle contraction. DATA COLLECTION AND ANALYSIS: Mean (standard deviation) cVEMP amplitude and asymmetry ratios were calculated for each muscle-contraction condition. Repeated measures analysis of variance and t-tests compared the variability in cVEMP amplitude between sides and conditions. Linear regression analyses compared asymmetry ratios. Polynomial regression analyses described the corrected and uncorrected cVEMP amplitude growth functions. RESULTS: While cVEMP amplitude increased with increased muscle contraction, the relationship was not linear or even proportionate. In the majority of cases, once muscle contraction reached a certain "threshold" level, cVEMP amplitude increased rapidly and then saturated. Normalizing cVEMP amplitudes did not remove the relationship between cVEMP amplitude and muscle contraction level. As muscle contraction increased, the normalized amplitude increased, and then decreased, corresponding with the observed amplitude saturation. Abnormal asymmetry ratios (based on values reported in the literature) were noted for four instances of uncorrected amplitude asymmetry at less than maximum muscle contraction levels. Amplitude normalization did not substantially change the number of observed asymmetry ratios. CONCLUSIONS: Because cVEMP amplitude did not typically grow proportionally with muscle contraction level, amplitude normalization did not lead to stable cVEMP amplitudes or asymmetry ratios across varying muscle contraction levels. Until we better understand the relationships between muscle contraction level, surface electromyography (EMG) estimates of muscle contraction level, and cVEMP amplitude, the application of normalization methods to correct cVEMP amplitude appears unjustified.

The auditory steady state response: far-field recordings from the chinchilla.

OBJECTIVE: Previous studies in our lab have found that the presentation of multiple ASSR-generating stimuli results in a decrease in ASSR amplitude when recorded from an electrode implanted in the chinchilla inferior colliculus. The purpose of the present experiment was to determine whether this same effect occurs in far-field recordings, i.e. recordings similar to those made in human subjects. The effect of inhalant anesthesia on ASSR amplitude in response to multiple stimuli was also investigated. DESIGN: Stimuli consisted of three sinusoidally-amplitude modulated tones with carrier/modulation frequencies of (1/.095 kHz), (2/.1 kHz), or (4/.107 kHz). The modulated carriers were presented to the right ear either alone or in combination, while recordings were made from subdermal needle electrodes placed on the head. STUDY SAMPLE: Nine adult chinchillas. RESULTS: A 20%-70% decrease in the response amplitude with the presentation of multiple ASSR-generating stimuli was found, which depended on both carrier frequency as well as stimulus pairing. In general, both the ASSR and the noise floor were reduced under anesthesia. CONCLUSIONS: The time savings obtained from presenting multiple stimuli simultaneously may not be as great as initially predicted, as the time saving is at least partially offset by the observed amplitude reduction.