Revealing the mechanism of novel nitrogen-doped biochar supported magnetite (NBM) enhancing anaerobic digestion of waste-activated sludge by sludge characteristics.

Anaerobic digestion (AD) is a promising technology in waste treatment and energy recovery. However, it suffers from long retention time and low biogas yield. In this study, novel nitrogen-doped biochar supported magnetite (NBM) was synthesized and applied to enhance the AD of waste-activated sludge. Results showed that NBM increased cumulative methane production and SCOD removal efficiency by up to 1.75 times and 15% respectively at 5 g/L compared with the blank. NBM enhanced both hydrolysis and methanogenesis process during AD and the activities of α-glucosidase, protease, coenzyme F420 and electron transport system were increased by 19%, 163%, 104% and 160% respectively at 5 g/L NBM compared with the blank. NBM also facilitated the secretion of conductive protein in extracellular polymeric substances as well as the formation of conductive pili, leading to 3.18-7.59 times higher sludge electrical conductivity. Microbial community analysis revealed that bacteria Clostridia and archaea Methanosarcina and Methanosaeta were enriched by the addition of NBM, and direct interspecies electron transfer might be promoted between them. This study provides a practical reference for future material synthesis and its application.

Enhanced acidogenic fermentation from Al-rich waste activated sludge by combining lysozyme and sodium citrate pretreatment: Perspectives of Al stabilization and enzyme activity.

The accumulation of poly aluminum chloride (PAC) in dewatered waste activated sludge (WAS) can cause severe Al pollution and significantly reduce the production of volatile fatty acids (VFAs) from anaerobic fermentation. Herein, the combination of lysozyme and sodium citrate pretreatment was applied to stabilize the aluminum and enhance the VFAs production via anaerobic fermentation. The complexation and stabilization of aluminum by the citrate was efficient, which is conducive to relieving the inhibition of aluminum on lysozymes and other extracellular hydrolases. Compared with the control group, the lysozyme, protease and α-glucosidase activities were obtained at 1.86, 1.72, and 1.15 times, respectively, following the pretreatment. 129.71 mg/g volatile suspended solids (VSS) of soluble proteins and 26.3 mg/g VSS of polysaccharides were obtained within 4 h, together with the degradation of 124 % more proteins and 75 % more polysaccharides within three days. This provided a sufficient number of substrates for VFA production. 588.4 mg COD/g VSS of total VFAs were obtained after the six-day anaerobic fermentation from Al-rich WAS following the combination of lysozyme and sodium citrate pretreatment, which was 7.3 times higher than that of the control group. This study presents a novel approach for enhancing VFA production in anaerobic fermentation as well as reducing risk of Al hazards from Al-rich WAS.