RESUMO
Nonapoptotic forms of cell death may facilitate the selective elimination of some tumor cells or be activated in specific pathological states. The oncogenic RAS-selective lethal small molecule erastin triggers a unique iron-dependent form of nonapoptotic cell death that we term ferroptosis. Ferroptosis is dependent upon intracellular iron, but not other metals, and is morphologically, biochemically, and genetically distinct from apoptosis, necrosis, and autophagy. We identify the small molecule ferrostatin-1 as a potent inhibitor of ferroptosis in cancer cells and glutamate-induced cell death in organotypic rat brain slices, suggesting similarities between these two processes. Indeed, erastin, like glutamate, inhibits cystine uptake by the cystine/glutamate antiporter (system x(c)(-)), creating a void in the antioxidant defenses of the cell and ultimately leading to iron-dependent, oxidative death. Thus, activation of ferroptosis results in the nonapoptotic destruction of certain cancer cells, whereas inhibition of this process may protect organisms from neurodegeneration.
Assuntos
Morte Celular , Ferro/metabolismo , Animais , Morte Celular/efeitos dos fármacos , Cicloexilaminas/farmacologia , Fibroblastos/citologia , Fibroblastos/metabolismo , Ácido Glutâmico/metabolismo , Hipocampo/citologia , Humanos , Técnicas In Vitro , Metabolismo dos Lipídeos , Neoplasias/patologia , Fenilenodiaminas/farmacologia , Piperazinas/metabolismo , Ratos , Espécies Reativas de Oxigênio/metabolismoRESUMO
In choanoflagellates, the closest living relatives of animals, multicellular rosette development is regulated by environmental bacteria. The simplicity of this evolutionarily relevant interaction provides an opportunity to identify the molecules and regulatory logic underpinning bacterial regulation of development. We find that the rosette-inducing bacterium Algoriphagus machipongonensis produces three structurally divergent classes of bioactive lipids that, together, activate, enhance, and inhibit rosette development in the choanoflagellate Salpingoeca rosetta. One class of molecules, the lysophosphatidylethanolamines (LPEs), elicits no response on its own but synergizes with activating sulfonolipid rosette-inducing factors (RIFs) to recapitulate the full bioactivity of live Algoriphagus. LPEs, although ubiquitous in bacteria and eukaryotes, have not previously been implicated in the regulation of a host-microbe interaction. This study reveals that multiple bacterially produced lipids converge to activate, enhance, and inhibit multicellular development in a choanoflagellate.
Assuntos
Proteínas de Bactérias/fisiologia , Bacteroidetes/fisiologia , Coanoflagelados/fisiologia , Proteínas de Bactérias/isolamento & purificação , Lipídeos/fisiologiaRESUMO
The choanoflagellate Salpingoeca rosetta is a microbial marine eukaryote that can switch between unicellular and multicellular states. As one of the closest living relatives of animals, this organism has become a model for understanding how multicellularity evolved in the animal lineage. Previously our laboratories isolated and synthesized a bacterially produced sulfonolipid that induces S. rosetta to form multicellular "rosettes." In this study, we report the identification of a bacterially produced inhibitor of rosettes (IOR-1) as well as the total synthesis of this molecule and all of its stereoisomers. Our results confirm the previously noted specificity and potency of rosette-modulating molecules, expand our understanding of the complex chemical ecology between choanoflagellates and rosette-inducing bacteria, and provide a synthetic probe template for conducting further mechanistic studies on the emergence of multicellularity.
Assuntos
Coanoflagelados , Lipídeos/farmacologia , Formação de Roseta/efeitos adversos , Animais , Coanoflagelados/efeitos dos fármacos , Coanoflagelados/crescimento & desenvolvimento , Lipídeos/isolamento & purificação , Biologia Marinha , EstereoisomerismoRESUMO
Stable multipartite mutualistic associations require that all partners benefit. We show that a single mutational step is sufficient to turn a symbiotic bacterium from an inedible but host-beneficial secondary metabolite producer into a host food source. The bacteria's host is a "farmer" clone of the social amoeba Dictyostelium discoideum that carries and disperses bacteria during its spore stage. Associated with the farmer are two strains of Pseudomonas fluorescens, only one of which serves as a food source. The other strain produces diffusible small molecules: pyrrolnitrin, a known antifungal agent, and a chromene that potently enhances the farmer's spore production and depresses a nonfarmer's spore production. Genome sequence and phylogenetic analyses identify a derived point mutation in the food strain that generates a premature stop codon in a global activator (gacA), encoding the response regulator of a two-component regulatory system. Generation of a knockout mutant of this regulatory gene in the nonfood bacterial strain altered its secondary metabolite profile to match that of the food strain, and also, independently, converted it into a food source. These results suggest that a single mutation in an inedible ancestral strain that served a protective role converted it to a "domesticated" food source.
Assuntos
Proteínas de Bactérias/genética , Dictyostelium/fisiologia , Mutação , Pseudomonas fluorescens/genética , Sequência de Aminoácidos , Proteínas de Bactérias/metabolismo , Benzopiranos/química , Benzopiranos/metabolismo , Cromatografia Líquida de Alta Pressão , Códon sem Sentido , Dictyostelium/metabolismo , Dictyostelium/microbiologia , Genes Reguladores/genética , Interações Hospedeiro-Patógeno , Espectroscopia de Ressonância Magnética , Dados de Sequência Molecular , Estrutura Molecular , Filogenia , Pseudomonas fluorescens/classificação , Pseudomonas fluorescens/fisiologia , Pirrolnitrina/química , Pirrolnitrina/metabolismo , Homologia de Sequência de Aminoácidos , Esporos de Protozoários/metabolismo , Esporos de Protozoários/fisiologiaRESUMO
This Viewpoint article provides a brief and selective summary of research on the chemical ecology underlying symbioses between bacteria and animals. Animals engage in multiple highly specialized interactions with bacteria that reflect their long coevolutionary history. The article focuses on a few illustrative but hardly exhaustive examples in which bacterially produced small molecules initiate a developmental step with important implications for the evolution of animals, provide signals for the maturation of mammalian immune systems, and furnish chemical defenses against microbial pathogens.
Assuntos
Ecologia/tendências , Simbiose , Animais , Bactérias/genética , Evolução Biológica , Humanos , Estrutura MolecularRESUMO
Studies on the origin of animal multicellularity have increasingly focused on one of the closest living relatives of animals, the choanoflagellate Salpingoeca rosetta. Single cells of S. rosetta can develop into multicellular rosette-shaped colonies through a process of incomplete cytokinesis. Unexpectedly, the initiation of rosette development requires bacterially produced small molecules. Previously, our laboratories reported the planar structure and femtomolar rosette-inducing activity of one rosette-inducing small molecule, dubbed rosette-inducing factor 1 (RIF-1), produced by the Gram-negative Bacteroidetes bacterium Algoriphagus machipongonensis. RIF-1 belongs to the small and poorly explored class of sulfonolipids. Here, we report a modular total synthesis of RIF-1 stereoisomers and structural analogs. Rosette-induction assays using synthetic RIF-1 stereoisomers and naturally occurring analogs defined the absolute stereochemistry of RIF-1 and revealed a remarkably restrictive set of structural requirements for inducing rosette development.
Assuntos
Ácidos Alcanossulfônicos/síntese química , Bacteroidetes/metabolismo , Coanoflagelados/efeitos dos fármacos , Lipídeos/síntese química , Morfogênese , Ácidos Alcanossulfônicos/química , Ácidos Alcanossulfônicos/farmacologia , Coanoflagelados/crescimento & desenvolvimento , Coanoflagelados/ultraestrutura , Lipídeos/química , Lipídeos/farmacologia , Estrutura Molecular , EstereoisomerismoRESUMO
Glucocorticoids are one of the most utilized and effective therapies in treating T-cell acute lymphoblastic leukemia. However, patients often develop resistance to glucocorticoids, rendering these therapies ineffective. We screened 9517 compounds, selected for their lead-like properties, chosen from among 3 372 615 compounds, against a dexamethasone-resistant T-ALL cell line to identify small molecules that reverse glucocorticoid resistance. We synthesized analogues of the most effective compound, termed J9, from the screen in order to define the scaffold's structure-activity relationship. Active compounds restored sensitivity to glucocorticoids through upregulation of the glucocorticoid receptor. This compound and mechanism may provide a strategy for overcoming glucocorticoid resistance in patients with T-ALL.