All-in-one continuous electrochemical monitoring of 2-phenylphenol removal from water by electro-Fenton treatment.

The biggest allure of heterogeneous electro-Fenton (HEF) processes largely fails on its high efficiency for the degradation of a plethora of hazardous compounds present in water, but still challenging to search for good and cost-effective electrocatalyst. In this work, carbon black (CB) and oxidised carbon black (CBox) materials were investigated as cathodes in the electrochemical production of hydrogen peroxide involved in HEF reaction for the degradation of 2-phenylphenol (2PP) as a target pollutant. The electrodes were fabricated by employing carbon cloth as support, and the highest H2O2 production yields were obtained for the CBox, pointing out the beneficial effect of the hydrophilic character of the electrode and oxygen-type functionalization of the carbonaceous surface. HEF degradation of 2PP was explored at -0.7 V vs. Ag/AgCl exhibiting the best conversion rates and degradation grade (total organic carbon) for the CBox-based cathode. In addition, the incorporation of an electrochemical sensor of 2PP in line with the HEF reactor was accomplished by the use of screen-printed electrodes (SPE) in order to monitor the pollutant degradation. The electrochemical sensor performance was evaluated from the oxidation of 2PP in the presence of Fe2+ ions by using square wave voltammetry (SWV) technique. The best electrochemical sensor performance was based on SPE modified with Meldola Blue showing a high sensitivity, low detection limit (0.12 ppm) and wide linear range (0.5-21 ppm) with good reproducibility (RSD 2.3 %). The all-in-one electrochemical station has been successfully tested for the degradation and quantification of 2PP, obtaining good recoveries analysing spiked waters from different water matrices origins.

Continuous Ketone Monitoring via Wearable Microneedle Patch Platform.

Ketone bodies (KBs), especially ß-hydroxybutyrate (BHB), have gained tremendous attention as potential biomarkers as their presence in bodily fluids is closely associated with health and wellness. While a variety of blood fingerstick test strips are available for self-testing of BHB, there are major needs for wearable devices capable of continuously tracking changing BHB concentrations. To address these needs, we present here the first demonstration of a wearable microneedle-based continuous ketone monitoring (CKM) in human interstitial fluid (ISF) and illustrate its ability to closely follow the intake of ketone drinks. To ensure highly stable and selective continuous detection of ISF BHB, the new enzymatic microneedle BHB sensor relies on a gold-coated platinum working electrode modified with a reagent layer containing toluidine blue O (TBO) redox mediator, ß-hydroxybutyrate dehydrogenase (HBD) enzyme, a nicotinamide adenine dinucleotide (NAD+) cofactor, along with carbon nanotubes (CNTs), chitosan (Chit), and a poly(vinyl chloride) (PVC) outer protective layer. The skin-worn microneedle sensing device operates with a miniaturized electrochemical analyzer connected wirelessly to a mobile electronic device for capturing, processing, and displaying the data. Cytotoxicity and skin penetration studies indicate the absence of potential harmful effects. A pilot study involving multiple human subjects evaluated continuous BHB monitoring in human ISF, against gold standard BHB meter measurements, revealing the close correlation between the two methods. Such microneedle-based CKM offers considerable promise for dynamic BHB tracking toward the management of diabetic ketoacidosis and personal nutrition and wellness.

Percepción de directivos de programas de atención domiciliaria en el departamento del Meta / Perception of Managers of Home Care Programs in the Department of Meta / Percepção dos Gestores de Programas de Atendimento Domiciliar no Departamento de Meta

Resumen El objetivo del trabajo realizado fue conocer la percepción de los directivos de los programas de Atención Domiciliaria (A.D), sobre el modelo que desarrollan como alternativa asistencial, así mismo analizar cómo se constituye la estructura del cuidado desde la perspectiva de las instituciones prestadoras de este servicio en el Departamento del Meta. El estudio fue cualitativo, descriptivo con enfoque fenomenológico, la población estuvo constituida por directivos de 8 programas de A.D existentes en los municipios de mayor desarrollo socioeconómico de la región. La información se obtuvo a través de entrevistas y narrativas, analizadas con la herramienta informática NVivo 10, la cual permitió la organización, análisis de la información y la interpretación de resultados a partir de la agrupación de temas centrales. Los resultados permitieron identificar tres categorías de análisis: Calidad y satisfacción del usuario, integralidad de la atención y Autocuidado como base del crecimiento personal. Se pudo concluir que la percepción encontrada en los directivos, está fundamentada en una intencionalidad que favorece el desarrollo de un modelo de atención integral participativo, que facilita el crecimiento personal, el fortalecimiento del autocuidado y el empoderamiento de los usuarios para acompañar diferentes situaciones de salud en el ámbito domiciliario.

Abstract The aim of the work was to study the perceptions of managers of programs of home care (A.D), on the model developed as alternative care, also analyze how is the structure of care from the perspective of institutions providing this service in the Department of Meta. The study was descriptive, qualitative phenomenological approach, the population consisted of Directors of 8 A.D existing programmes in the municipalities of greater socio-economic development of the region. The information was obtained through interviews and narratives, analyzed with NVivo 10 computer tool, which allowed the Organization, analysis of the information and the interpretation of results from the grouping of central themes. The results helped identify three categories of analysis: quality and user satisfaction, integrality of care and self-care as a basis for personal growth. One could conclude that perception found in the executives, is based on the intention that favours the development of a participatory model of comprehensive care that facilitates personal growth, the strengthening of self-care and the empowerment of users to accompany different situations of health at the home.

Resumo O objetivo do trabalho foi estudar a percepção dos gestores de programas de atendimento domiciliar (AD), o modelo desenvolvido como alternativa de cuidados, também analisar como é a estrutura de atendimento da perspectiva de instituições oferecendo esse serviço no departamento de Meta. O estudo foi descritiva, qualitativa de abordagem fenomenológica, a população era formada por diretores de Adriano 8 programas existentes nos municípios de maior desenvolvimento sócio-económico da região. As informações foram obtidas através de entrevistas e narrativas, analisadas com NVivo 10 ferramenta de computador, que permitiu a organização, análise da informação e a interpretação dos resultados do agrupamento de temas centrais. Os resultados ajudaram a identificar três categorias de análise: qualidade e satisfação dos utilizadores, a integralidade do cuidado e auto-cuidado como base para o crescimento pessoal. Se poderia concluir que percepção encontrada nos executivos, baseia-se sobre a intenção que favorece o desenvolvimento de um modelo participativo de atenção integral que facilita o crescimento pessoal, o reforço de auto-cuidado e a empoderamento dos usuários para acompanhar as diferentes situações de saúde em casa.

Mutagénesis con luz ultravioleta (UV) de una cepa del género streptomyces productora de antibiótico / Ultraviolet mutagenesis of an antibiotic-producing straing of the genre streptomyces

Se aíslan 6 mutantes auxotróficos por la técnica de plaqueo por réplica después de la mutagénesis con luz ultravioleta (UV). Se señala que en la caracterización todos resultaron alterados en la vía de síntesis de la arginina. Se aíslan 4 revertantes espontáneos de 3 de los mutantes. Se informa que la aplicación del método de Kelner de difusión en agar para determinar la producción antibiótica a mutantes y revertantes y el procesamiento estadístico de los resultados obtenidos, evidenció que no existen diferencias significativas entre la cepa parental y los mutantes, pero sí entre la cepa parental y los revertantes, y produce uno de ellos, el 28-2, significativamente menos que la cepa RA-2. Se indica que el cálculo del coeficiente de variación para los mutantes evidenció una mayor estabilidad de los mutantes 20, 25, 26, y 28 en relación con la cepa parental, y el 25 es 5 veces más estable que éste

Incremento en la producción de eritromicina por fusión de protoplastos de Streptomyces erythreus / Increasing in the production of erythromycin by fusion of protoplasts of Streptomyces erythreus

El método de fusión de protoplastos fue usado para la obtención de recombinantes con producción antibiótica incrementada en Streptomyces erythreus productora de eritromicina. Se emplearon los mutantes morfológicos 36 UV y P-E obtenidos por luz UV y luz UV-acriflavina respectivamente, los cuales poseían marcadores de resistencia a cloranfenicol y rifampicina. Se establecen las condiciones más apropiadas para la obtención y fusión de protoplastos en estas cepas, llegándose a obtener recombinantes resistentes a ambos antibióticos a frecuencia de 0,57 %, dentro de las cuales fue seleccionado uno con incremento de 4 veces en la producción de eritromicina

Formación y regeneración de protoplastos en streptomyces erythreus productora de eritromicina / Protoplast relese and regeneration in erythromycin producing streptomyces erythreus

Se establecen las condiciones necesarias para la formación de protoplastos en Streptomyces erythreus con el uso del tratamiento enzimático con lisozima, además del medio específico y de las condiciones más efectivas para la regeneración de los mismos