Does a Mediterranean-type dietary pattern exert a cardio-protective effect outside the Mediterranean region? A review of current evidence.

Current evidence suggests a beneficial effect of the Mediterranean diet (MD) on human health. This has led to a rise in studies investigating the role of the MD in cardiovascular disease (CVD) prevention outside the region of its origin. We aimed to outline the evolving understanding of the MD and to provide an overview of adherence to MD in non-Mediterranean countries and the modulatory effects of MD on CVD biomarkers. Evidence suggest that MD may exert a protective effect on CVD via ameliorating blood lipid profile and blood pressure. A protective effect of MD was found with regard to left ventricular mass but data on carotid intima media thickness and pulse wave velocity are lacking. The paucity of data does not give a clear picture of the health effects of the MD in non-Mediterranean countries, thus larger and well-designed studies on MD outside the Mediterranean countries are warranted.

Cognitive dissonance in food and nutrition-A review.

The study of cognitive dissonance in food and nutrition has been relatively under-developed. This review paper looks at food and/or food-related studies that have utilized cognitive dissonance as a primary construct in a priori theorization and hypothesis-formulation, examining the ways in which the dissonance construct has been used and its corresponding effects on various food-related outcomes in those studies. Current gaps and critical issues underlying cognitive dissonance investigation in food and nutrition research are also identified and discussed.

A Central Asian Food Dataset for Personalized Dietary Interventions.

Nowadays, it is common for people to take photographs of every beverage, snack, or meal they eat and then post these photographs on social media platforms. Leveraging these social trends, real-time food recognition and reliable classification of these captured food images can potentially help replace some of the tedious recording and coding of food diaries to enable personalized dietary interventions. Although Central Asian cuisine is culturally and historically distinct, there has been little published data on the food and dietary habits of people in this region. To fill this gap, we aim to create a reliable dataset of regional foods that is easily accessible to both public consumers and researchers. To the best of our knowledge, this is the first work on the creation of a Central Asian Food Dataset (CAFD). The final dataset contains 42 food categories and over 16,000 images of national dishes unique to this region. We achieved a classification accuracy of 88.70% (42 classes) on the CAFD using the ResNet152 neural network model. The food recognition models trained on the CAFD demonstrate the effectiveness and high accuracy of computer vision for dietary assessment.

Reducing meat consumption in Central Asia through 3D printing of plant-based protein-enhanced alternatives-a mini review.

3D food printing (3DFP) is emerging as a vital innovation in the food industry's pursuit of sustainability. 3DFP has evolved to significantly impact food production, offering the capability to create customized, nutritionally balanced foods. Central Asia has a higher than global average level of meat consumption per capita, which might be influenced by its historical and cultural background of nomadism. This dietary trend might potentially result in negative impacts on both the environment and human health outcomes, as it leads to increased greenhouse gas emissions and increased risk of chronic diseases. Reducing meat consumption holds the potential to address these sustainability and health issues. A possible strategy to reduce meat consumption and promote plant-based foods is 3D Food Printing (3DFP), which can rely on plant-protein sources from the region to create appealing and tasty alternatives for these populations. This review summarizes recent studies on plant protein-rich materials for 3DFP as a substitute to meet the growing global demand for meat as well as the 3DFP printing parameters associated with the different plant-based proteins currently used (e.g., lentils, soybeans, peas, and buckwheat). The findings revealed that buckwheat, a dietary staple in Central Asia, can be a promising choice for 3DFP technology due to its widespread consumption in the region, gluten-free nature, and highly nutritious profile.

Proposing new body mass index and waist circumference cut-offs based on cardiometabolic risks for a Central Asia population: A feasibility study.

Background: Lower BMI cutoffs as compared to standard cut-offs have been recommended to reduce the risk of obesity-related co-morbidities in some ethnic populations (e.g. south Asian and Chinese populations). Recent attempts have also been made to establish ethnicity-specific BMI cutoffs to identify individuals affected with obesity in relation to type 2 diabetes risk in multi-ethnic populations based in the UK and North America. However, to date, there is yet to have any published work done to identify these cut-offs in Central Asia populations nor specify any difference for genders even though the fat distribution varies amongst the different ethnic groups as well as between the genders. To the best of the authors' knowledge, this is the first study exploring new BMI and WC cut-offs in this population. Methods: To address this gap, we used a database of secondary care electronic health records from the National Research Cardiac Surgery Center to identify BMI and waist circumference cutoffs for obesity based on the risk of developing diabetes and other cardiometabolic disorders among 297 adults in Kazakhstan. Bivariate and multivariable logistic regression analysis were utilized to investigate the relationships between risk factors and type 2 diabetes mellitus (T2DM). BMI and WC thresholds were predicted using the Youden index. Results: For an equivalent age-adjusted and sex-adjusted incidence of type 2 diabetes at a BMI of 30·0 kg/m2 in White populations, we found higher BMI cutoffs for Kazakhstani women (30.5 kg/m2) but lower cut-offs for men (28·9 kg/m2). As for waist circumference, the cut-off points for females were 95cm and 104 cm for males. Conclusions: For Central Asia populations, the current recommended BMI and WC cutoffs may not be suitable and further work is needed to establish specific cut-offs for this population.

Genetic factors associated with obesity risks in a Kazakhstani population.

OBJECTIVES: There is limited published literature on the genetic risks of chronic inflammatory related disease (eg, obesity and cardiovascular disease) among the Central Asia population. The aim is to determine potential genetic loci as risk factors for obesity for the Kazakhstani population. SETTING: Kazakhstan. PARTICIPANTS: One hundred and sixty-three Kazakhstani nationals (ethnic groups: both Russians and Kazakhs) were recruited for the cross-sectional study. Linear regression models, adjusted for confounding factors, were used to examine the genetic associations of single nucleotide polymorphisms (SNPs) in 19 genetic loci with obesity (73 obese/overweight individuals and 90 controls). RESULTS: Overall, logistic regression analyses revealed genotypes C/T in CRP (rs1205), A/C in AGTR1 (rs5186), A/G in CBS (rs234706), G/G in FUT2 (rs602662), A/G in PAI-1 (rs1799889), G/T (rs1801131) and A/G (rs1801133) in MTHFR genes significantly decrease risk of overweight/obesity. After stratification for ethnicity, rs234706 was significantly associated with overweight/obesity in both Russians and Kazakhs, while rs1800871 was significant in Kazakhs only. CONCLUSIONS: This study revealed that variations in SNPs known to be associated with cardiovascular health can also contribute to the risks of developing obesity in the population of Kazakhstan.

Effects of atherogenic diet and atorvastatin treatment on gene expression profiles in the C57BL/6J mouse liver.

This study investigated the early and long-term effects of atherogenic diet on hepatic gene expression, and the restorative effects of atorvastatin in treating hypercholesterolemia. Two groups of female C57BL/6J mice were fed standard chow or atherogenic diet for 1-week early phase study and two other groups for 10 weeks. The fifth group had daily 10 mg/kg atorvastatin injections for 3 weeks from week 8 of the atherogenic diet. Gene expression profiling was carried out with Affymetrix GeneChips. One-week atherogenic diet elevated 38 and inhibited 127 gene expressions, while 10-week atherogenic diet elevated 165 and inhibited 281 genes by more than twofold. Atorvastatin could restore 78.2% and 68%, respectively, of the genes to normal levels. Genes in the Insig (insulin-induced gene)-SREBP (sterol regulatory element binding proteins) pathway were mostly inhibited by atherogenic diet at week 1 but elevated at week 10. Of these, 65.2% were restored by atorvastatin. In conclusion, lipid homeostatic mechanism coped well with short-term atherogenic diet. However, when such a diet was prolonged, the mechanism was no longer effective but entered into a pathological state in which lipogenic genes, especially those in the Insig-SREBP pathway, were upregulated. Atorvastatin could restore changes in the Insig-SREBP pathway that were induced by the atherogenic diet.

Sequential effects of a high-fiber diet with psyllium husks on the expression levels of hepatic genes and plasma lipids.

OBJECTIVE: We studied the sequential effects of a high-fiber diet using psyllium husks on hepatic gene expression and plasma lipid levels. METHODS: C57BL/6J mice were randomly assigned to a control diet or a high-fiber diet containing 10% psyllium husks for 3 weeks (PE-3wk) and 10 weeks (PE-10wk). Oligonucleotide microarrays were used to screen the transcriptional response at both time points. Genes encoding enzymes regulating key steps of lipid metabolism were then selected for further validation by quantitative real-time reverse transcription polymerase chain reaction and their protein expression by western blot assays. RESULTS: Plasma cholesterol and triacylglycerol levels were reduced in both high-fiber groups. Three weeks of high-fiber feeding downregulated genes involved in lipogenesis, whereas those involved in cholesterol and bile acid synthesis were upregulated. With prolonged high-fiber feeding, genes involved in lipogenesis such as fatty acid synthase (Fasn) were then upregulated. Additional genes in cholesterol synthesis such as 3-hydroxy-3-methylglutaryl coenzyme A reductase (Hmgcr) were also upregulated. At week 3, protein expression levels of Fasn were significantly lower in the high-fiber group but increased at week 10. Protein levels of Hmgcr were significantly increased in PE-10wk mice. CONCLUSION: The high-fiber diet containing psyllium husks reduced plasma total cholesterol and triacylglycerol levels. Cholesterol lowering was most likely mediated by increased bile acid synthesis. The increased transcript levels of genes related to cholesterol synthesis throughout the entire feeding period and the subsequent increased lipogenic gene transcript levels could likely suggest a regulatory mechanism to restore the lowered plasma cholesterol and triacylglycerol levels.

The Driver Mutational Landscape of Ovarian Squamous Cell Carcinomas Arising in Mature Cystic Teratoma.

Purpose: We sought to identify the genomic abnormalities in squamous cell carcinomas (SCC) arising in ovarian mature cystic teratoma (MCT), a rare gynecological malignancy of poor prognosis.Experimental design: We performed copy number, mutational state, and zygosity analysis of 151 genes in SCC arising in MCT (n = 25) using next-generation sequencing. The presence of high-/intermediate-risk HPV genotypes was assessed by quantitative PCR. Genomic events were correlated with clinical features and outcome.Results: MCT had a low mutation burden with a mean of only one mutation per case. Zygosity analyses of MCT indicated four separate patterns, suggesting that MCT can arise from errors at various stages of oogenesis. A total of 244 abnormalities were identified in 79 genes in MCT-associated SCC, and the overall mutational burden was high (mean 10.2 mutations per megabase). No SCC was positive for HPV. The most frequently altered genes in SCC were TP53 (20/25 cases, 80%), PIK3CA (13/25 cases, 52%), and CDKN2A (11/25 cases, 44%). Mutation in TP53 was associated with improved overall survival. In 8 of 20 cases with TP53 mutations, two or more variants were identified, which were bi-allelic.Conclusions: Ovarian SCC arising in MCT has a high mutational burden, with TP53 mutation the most common abnormality. The presence of TP53 mutation is a good prognostic factor. SCC arising in MCT share similar mutation profiles to other SCC. Given their rarity, they should be included in basket studies that recruit patients with SCC of other organs. Clin Cancer Res; 23(24); 7633-40. ©2017 AACR.

Consumption of takeaway and fast food in a deprived inner London Borough: are they associated with childhood obesity?

OBJECTIVE: A major concern is the ubiquitous presence of fast food and takeaway outlets within easy walking distance of schools, particularly in the light of the increasing burden of childhood obesity. Here, the associations between the schoolchildren's weights, their consumption of fast food and takeaway outlets were examined in a deprived inner London Borough. DESIGN: This is a cross-sectional study. PARTICIPANTS: 193 schoolchildren (aged between 11 and 14 years old) participated in this study. MAIN OUTCOME MEASURES: Body mass index (BMI) percentiles specific for age and gender were obtained. Frequency of food and drinks purchased from fast food outlets and takeaway outlets over a weekly period and preferred types of drinks and food products usually consumed were measured. RESULTS: More than 50% of the children in our survey purchased food or drinks from fast food or takeaway outlets twice or more a week, with about 10% consuming fast food or drinks from these outlets daily. About 70% of these children from Black ethnic groups and 54% of Asians purchased fast food more than twice a week. BMI has a significantly inverse relationship to fast food consumption. However, when age and gender are accounted, the BMI age-gender percentile is no longer significantly related to fast food consumption. CONCLUSIONS: This study revealed a very high frequency of fast food consumption among the schoolchildren. Taste, quick access and peer influence were major contributing factors. These schoolchildren are exposed to an obesogenic environment, and it is not surprising that in this situation, many of these children are already overweight and will likely become obese as adults.

Characteristics, and disease control and complications of hypertensive patients in primary-care - a community-based study in Singapore.

INTRODUCTION: Hypertension is a common chronic condition usually managed by primary-care practitioners in Singapore. This study assessed the characteristics, control and complications of non-diabetic hypertensive patients managed at government primary healthcare clinics. MATERIALS AND METHODS: A cross-sectional study involving 9 clinics was conducted over 1-week in 2006. Five hundred and six non-diabetic hypertensive patients were systematically sampled from all clinic attendees. Data relating to socio-demographic, lifestyle factors, treatment and complications were collected by interviewer-administered questionnaires and review of clinic medical records. Blood pressure (BP) measurements were taken with validated automated sets following a standard protocol. RESULTS: The prevalence of good BP control (<140/90 mmHg) was 37.7% (95% CI: 33.6% to 41.8%). Ninety seven percent were on medication with about half on monotherapy. Seventy percent of patients had a body mass index (BMI) of 23.0 kg/m(2) or higher, 64% did not exercise regularly and 8% were current smokers. After adjusting for age and lifestyle factors, male hypertensive patients had poorer BP control compared to females. Nineteen percent of patients reported at least 1 complication of hypertension, especially cardiac disease. After multivariate analysis and duration of disease, age and the male gender were associated with the presence of hypertensive complications. CONCLUSIONS: More than half of the patients were not controlled to target levels. Male patients were more likely to have poorer control of hypertension and significantly higher risks of complications. Control of BP could be further improved by lifestyle modifications - weight reduction, promotion of physical activity, healthier eating habits and smoking cessation.

Sequential responses to high-fat and high-calorie feeding in an obese mouse model.

OBJECTIVE: Reports on the immediate and long-term responses to high-fat and high-calorie (HFC) feeding are controversial. Therefore, we examined the sequential effects of an HFC diet. METHODS AND PROCEDURES: C57BL/6J mice were randomly assigned to consume either the control (C) or the HFC diet. Body weights and food intake were measured weekly and other measurements at weeks 2, 4, and 10. Microarrays were used for screening the transcriptional response of the livers at the three time points. Genes, encoding enzymes regulating key steps of lipid metabolism, were then selected from the microarray data for validation by quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) and their protein expression by western blot assays. RESULTS: Mice fed with HFC diet for 2 weeks showed no increase in food intake and no difference in weight gain compared to the C mice. At weeks 4 and 10, the HFC mice increased their food intake and gained more weight than their controls (by 1.4 times and 2.5 times, respectively) (P<0.01 at week 10). Genes involved in fatty acid oxidation (FAO) were initially upregulated and then downregulated, whereas the lipogenic genes and genes involved in cholesterol synthesis showed reverse trends. The differential mRNA expression of Cpt1L, Fas, and Hmgcr were confirmed by RT-PCR and their protein expression by western blot assays. DISCUSSION: Our findings suggested that when mice were fed an HFC diet, they could develop initial compensatory response to resist the increased energy balance; however, a prolonged consumption of an HFC diet appeared to disrupt this adaptation.