RESUMO
The importance and need for eco-oriented technologies has increased worldwide, which leads to an enhanced development of methods for the synthesis of nanoparticles using biological agents. This review de-scribes the current approaches to the preparation of biogenic silver nanoparticles, using plant extracts and filtrates of fungi and microorganisms. The peculiarities of the synthesis of particles depending on the source of biocomponents are considered as well as physico-morphological, antibacterial and antifungal properties of the resulting nanoparticles which are compared with such properties of silver nanoparticles obtained by chemical synthesis. Special attention is paid to the process of self-assembly of biogenic silver nanoparticles.
RESUMO
The development of novel nanoscale vehicles for drug delivery promotes the growth of interest in investigations of interaction between nanomaterials. In this paper, we report the in vitro studies of eukaryotic cell physiological response to incubation with graphene oxide and planar kaolin nanoclay. Graphene family materials, including graphene oxide (GO), hold promise for numerous applications due to their unique electronic properties. However, graphene oxide reveals toxicity to some cell lines through an unidentified mechanism. Thus, methods and agents reducing the toxicity of graphene oxide can widen its practical application. We used a colorimetric test, flow cytometry and cell index assay methods to evaluate the effects of separate and combined application of graphene oxide and kaolin on mammalian cells. We have shown that the joint application of graphene oxide and kaolin reduced the negative effects of graphene by almost 20%, most likely because of coagulation of the nanoparticles with each other, which was detected by atomic force microscopy.