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1.
Proc Natl Acad Sci U S A ; 119(27): e2200109119, 2022 07 05.
Artigo em Inglês | MEDLINE | ID: mdl-35763573

RESUMO

Understanding the factors that influence the airborne survival of viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in aerosols is important for identifying routes of transmission and the value of various mitigation strategies for preventing transmission. We present measurements of the stability of SARS-CoV-2 in aerosol droplets (∼5 to 10 µm equilibrated radius) over timescales spanning 5 s to 20 min using an instrument to probe survival in a small population of droplets (typically 5 to 10) containing ∼1 virus/droplet. Measurements of airborne infectivity change are coupled with a detailed physicochemical analysis of the airborne droplets containing the virus. A decrease in infectivity to ∼10% of the starting value was observable for SARS-CoV-2 over 20 min, with a large proportion of the loss occurring within the first 5 min after aerosolization. The initial rate of infectivity loss was found to correlate with physical transformation of the equilibrating droplet; salts within the droplets crystallize at relative humidities (RHs) below 50%, leading to a near-instant loss of infectivity in 50 to 60% of the virus. However, at 90% RH, the droplet remains homogenous and aqueous, and the viral stability is sustained for the first 2 min, beyond which it decays to only 10% remaining infectious after 10 min. The loss of infectivity at high RH is consistent with an elevation in the pH of the droplets, caused by volatilization of CO2 from bicarbonate buffer within the droplet. Four different variants of SARS-CoV-2 were compared and found to have a similar degree of airborne stability at both high and low RH.


Assuntos
Partículas e Gotas Aerossolizadas , COVID-19 , SARS-CoV-2 , Partículas e Gotas Aerossolizadas/química , Partículas e Gotas Aerossolizadas/isolamento & purificação , COVID-19/transmissão , Humanos , Umidade , Concentração de Íons de Hidrogênio , SARS-CoV-2/isolamento & purificação , SARS-CoV-2/patogenicidade
2.
Parasitology ; 149(9): 1186-1192, 2022 08.
Artigo em Inglês | MEDLINE | ID: mdl-35570692

RESUMO

Nest boxes have been used for many decades as tools for conservation and to study avian population dynamics. Plastic is increasingly used as a material for nest boxes, but no studies have investigated effects of this different material. Two consecutive studies were conducted to investigate effects of nest-box environment on nidicolous parasites, bacteria and fungi, as well as nest success, in blue tits Cyanistes caeruleus and great tits Parus major. The first compared microclimate and parasite and pathogen load in plastic and wooden nest boxes. The second tested the nest protection hypothesis ­ that birds naturally incorporate aromatic herbs into nests to decrease nest parasites and pathogens ­ by comparing parasite and pathogen load in plastic nest boxes to which aromatic or non-aromatic plant material was added. No significant difference in nest-box temperature or relative humidity was found between plastic and wooden boxes. Wooden boxes, however, contained 30-fold higher numbers of fleas and a higher total bacterial load on chicks. Fledging success for blue tit broods was significantly higher in wooden boxes. Parasites and bacteria did not decrease by the inclusion of aromatic herbs. The results increase the evidence base for nest-box design in support of plastic, which can provide an appropriate alternative nest-box material to wood, with apparently no difference in microclimate and no increase in the load of measured parasites and pathogens.


Assuntos
Passeriformes , Animais , Bactérias , Fungos , Comportamento de Nidação , Passeriformes/parasitologia , Plásticos , Dinâmica Populacional
3.
J Appl Microbiol ; 132(4): 2633-2641, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34923720

RESUMO

AIMS: To investigate whether on-farm antibacterial usage (ABU), environmental antibacterial-resistant (ABR) Escherichia coli prevalence, sampling and sample handling methodologies are associated with ABR E. coli positivity in individual faecal samples from dairy heifers. METHODS AND RESULTS: Three hundred and sixty-four heifers from 37 farms were sampled via rectal or faecal pat sampling. Samples were stored at -80°C for variable periods before microbiological analysis. Data analysis was done through a multilevel, multivariable logistic regression approach. Individual rectal samples had increased odds of positivity for amoxicillin-, cefalexin- and tetracycline-resistant E. coli. Sample storage for 6-12 months was associated with decreased odds of finding amoxicillin- and tetracycline-resistant E. coli. On-farm ABU had little influence, and environmental ABR E. coli prevalence had no significant influence on the odds of sample-level positivity for ABR E. coli. CONCLUSIONS: Sampling methodology and sample handling have a greater association than on-farm factors with the detection of ABR E. coli in individual faecal samples from dairy heifers. SIGNIFICANCE AND IMPACT OF THE STUDY: Sampling and storage methodologies should be considered carefully at the point of designing ABR surveillance studies in livestock and their environments and, where possible, these methodologies should be standardized between and within future studies.


Assuntos
Doenças dos Bovinos , Infecções por Escherichia coli , Animais , Antibacterianos/farmacologia , Bovinos , Doenças dos Bovinos/microbiologia , Indústria de Laticínios , Escherichia coli , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/microbiologia , Infecções por Escherichia coli/veterinária , Fezes/microbiologia , Feminino , Tetraciclina
4.
J Antimicrob Chemother ; 76(12): 3144-3150, 2021 11 12.
Artigo em Inglês | MEDLINE | ID: mdl-34450630

RESUMO

BACKGROUND: Our primary aim was to test whether cattle-associated fluoroquinolone-resistant (FQ-R) Escherichia coli found on dairy farms are closely phylogenetically related to those causing bacteriuria in humans living in the same 50 × 50 km geographical region suggestive of farm-human sharing. Another aim was to identify risk factors for the presence of FQ-R E. coli on dairy farms. METHODS: FQ-R E. coli were isolated during 2017-18 from 42 dairy farms and from community urine samples. Forty-two cattle and 489 human urinary isolates were subjected to WGS, allowing phylogenetic comparisons. Risk factors were identified using a Bayesian regularization approach. RESULTS: Of 489 FQ-R human isolates, 255 were also third-generation-cephalosporin-resistant, with strong genetic linkage between aac(6')Ib-cr and blaCTX-M-15. We identified possible farm-human sharing for pairs of ST744 and ST162 isolates, but minimal core genome SNP distances were larger between farm-human pairs of ST744 and ST162 isolates (71 and 63 SNPs, respectively) than between pairs of isolates from different farms (7 and 3 SNPs, respectively). Total farm fluoroquinolone use showed a positive association with the odds of isolating FQ-R E. coli, while total dry cow therapy use showed a negative association. CONCLUSIONS: This work suggests that FQ-R E. coli found on dairy farms have a limited impact on community bacteriuria within the local human population. Reducing fluoroquinolone use may reduce the on-farm prevalence of FQ-R E. coli and this reduction may be greater when dry cow therapy is targeted to the ecology of resistant E. coli on the farm.


Assuntos
Bacteriúria , Infecções por Escherichia coli , Animais , Antibacterianos/farmacologia , Teorema de Bayes , Bovinos , Escherichia coli/genética , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/veterinária , Fazendas , Feminino , Fluoroquinolonas/farmacologia , Humanos , Filogenia
5.
Appl Environ Microbiol ; 87(6)2021 02 26.
Artigo em Inglês | MEDLINE | ID: mdl-33397699

RESUMO

Little is known about the drivers of critically important antibacterial resistance in species with zoonotic potential present on farms (e.g., CTX-M ß-lactamase-positive Escherichia coli). We collected samples monthly between January 2017 and December 2018 on 53 dairy farms in South West England, along with data for 610 variables concerning antibacterial usage, management practices, and meteorological factors. We detected E. coli resistant to amoxicillin, ciprofloxacin, streptomycin, and tetracycline in 2,754/4,145 (66%), 263/4,145 (6%), 1,475/4,145 (36%), and 2,874/4,145 (69%), respectively, of samples from fecally contaminated on-farm and near-farm sites. E. coli positive for blaCTX-M were detected in 224/4,145 (5.4%) of samples. Multilevel, multivariable logistic regression showed antibacterial dry cow therapeutic choice (including use of cefquinome or framycetin) to be associated with higher odds of blaCTX-M positivity. Low average monthly ambient temperature was associated with lower odds of blaCTX-ME. coli positivity in samples and with lower odds of finding E. coli resistant to each of the four test antibacterials. This was in addition to the effect of temperature on total E. coli density. Furthermore, samples collected close to calves had higher odds of having E. coli resistant to each antibacterial, as well as E. coli positive for blaCTX-M Samples collected on pastureland had lower odds of having E. coli resistant to amoxicillin or tetracycline, as well as lower odds of being positive for blaCTX-MIMPORTANCE Antibacterial resistance poses a significant threat to human and animal health and global food security. Surveillance for resistance on farms is important for many reasons, including tracking impacts of interventions aimed at reducing the prevalence of resistance. In this longitudinal survey of dairy farm antibacterial resistance, we showed that local temperature-as it changes over the course of a year-was associated with the prevalence of antibacterial-resistant E. coli We also showed that prevalence of resistant E. coli was lower on pastureland and higher in environments inhabited by young animals. These findings have profound implications for routine surveillance and for surveys carried out for research. They provide important evidence that sampling at a single time point and/or single location on a farm is unlikely to be adequate to accurately determine the status of the farm regarding the presence of samples containing resistant E. coli.


Assuntos
Farmacorresistência Bacteriana , Escherichia coli/genética , beta-Lactamases/genética , Envelhecimento , Amoxicilina/farmacologia , Animais , Antibacterianos/farmacologia , Bovinos , Doenças dos Bovinos/microbiologia , Ciprofloxacina/farmacologia , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/microbiologia , Fazendas , Fezes/microbiologia , Estreptomicina/farmacologia , Temperatura , Tetraciclina/farmacologia
6.
Appl Environ Microbiol ; 87(1)2020 12 17.
Artigo em Inglês | MEDLINE | ID: mdl-33067197

RESUMO

Third-generation cephalosporin resistance (3GC-R) in Escherichia coli is a rising problem in human and farmed-animal populations. We conducted whole-genome sequencing analysis of 138 representative 3GC-R isolates previously collected from dairy farms in southwest England and confirmed by PCR to carry acquired 3GC-R genes. This analysis identified blaCTX-M (131 isolates encoding CTX-M-1, -14, -15, -and 32 and the novel variant CTX-M-214), blaCMY-2 (6 isolates), and blaDHA-1 (1 isolate). A highly conserved plasmid was identified in 73 isolates, representing 27 E. coli sequence types. This novel ∼220-kb IncHI2 plasmid carrying blaCTX-M-32 was sequenced to closure and designated pMOO-32. It was found experimentally to be stable in cattle and human transconjugant E. coli even in the absence of selective pressure and was found by multiplex PCR to be present on 26 study farms representing a remarkable range of transmission over 1,500 square kilometers. However, the plasmid was not found among human urinary E. coli isolates we recently characterized from people living in the same geographical location, collected in parallel with farm sampling. There were close relatives of two blaCTX-M plasmids circulating among eight human and two cattle isolates, and a closely related blaCMY-2 plasmid was found in one cattle and one human isolate. However, phylogenetic evidence of recent sharing of 3GC-R strains between farms and humans in the same region was not found.IMPORTANCE Third-generation cephalosporins (3GCs) are critically important antibacterials, and 3GC resistance (3GC-R) threatens human health, particularly in the context of opportunistic pathogens such as Escherichia coli There is some evidence for zoonotic transmission of 3GC-R E. coli through food, but little work has been done examining possible transmission via interaction of people with the local near-farm environment. We characterized acquired 3GC-R E. coli found on dairy farms in a geographically restricted region of the United Kingdom and compared these with E. coli from people living in the same region, collected in parallel. While there is strong evidence for recent farm-to-farm transmission of 3GC-R strains and plasmids-including one epidemic plasmid that has a remarkable capacity to be transmitted-there was no evidence that 3GC-R E. coli found on study farms had a significant impact on circulating 3GC-R E. coli strains or plasmids in the local human population.


Assuntos
Doenças dos Bovinos/transmissão , Infecções por Escherichia coli/veterinária , Escherichia coli/fisiologia , beta-Lactamases/genética , Animais , Bovinos , Doenças dos Bovinos/epidemiologia , Inglaterra/epidemiologia , Escherichia coli/enzimologia , Escherichia coli/genética , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/transmissão , Epidemiologia Molecular , Plasmídeos/genética , Plasmídeos/metabolismo , beta-Lactamases/metabolismo
8.
Infect Immun ; 83(12): 4884-95, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26438798

RESUMO

Campylobacter jejuni is a commensal bacterium in the intestines of animals and birds and a major cause of food-borne gastroenteritis in humans worldwide. Here we show that exposure to pancreatic amylase leads to secretion of an α-dextran by C. jejuni and that a secreted protease, Cj0511, is required. Exposure of C. jejuni to pancreatic amylase promotes biofilm formation in vitro, increases interaction with human epithelial cell lines, increases virulence in the Galleria mellonella infection model, and promotes colonization of the chicken ileum. We also show that exposure to pancreatic amylase protects C. jejuni from stress conditions in vitro, suggesting that the induced α-dextran may be important during transmission between hosts. This is the first evidence that pancreatic amylase functions as an interkingdom signal in an enteric microorganism.


Assuntos
Proteínas de Bactérias/genética , Biofilmes/efeitos dos fármacos , Infecções por Campylobacter/veterinária , Campylobacter jejuni/efeitos dos fármacos , alfa-Amilases Pancreáticas/farmacologia , Peptídeo Hidrolases/genética , Doenças das Aves Domésticas/microbiologia , Animais , Proteínas de Bactérias/metabolismo , Biofilmes/crescimento & desenvolvimento , Células CACO-2 , Infecções por Campylobacter/enzimologia , Infecções por Campylobacter/microbiologia , Infecções por Campylobacter/patologia , Campylobacter jejuni/patogenicidade , Campylobacter jejuni/fisiologia , Linhagem Celular Tumoral , Galinhas , Dextranos/biossíntese , Dextranos/metabolismo , Células Epiteliais , Regulação da Expressão Gênica , Interações Hospedeiro-Patógeno , Humanos , Intestinos/microbiologia , Intestinos/patologia , Mariposas/microbiologia , alfa-Amilases Pancreáticas/isolamento & purificação , Peptídeo Hidrolases/metabolismo , Doenças das Aves Domésticas/enzimologia , Doenças das Aves Domésticas/patologia , Transdução de Sinais , Suínos
9.
Curr Microbiol ; 67(3): 333-40, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23636493

RESUMO

Control of Campylobacter in the food chain requires a better understanding of the behaviour of the bacteria in relevant environments. Campylobacter species are largely non-pathogenic in poultry, the body temperature of which is 42 °C. However, the bacteria are highly pathogenic in humans whose body temperature is 37 °C. The aim of this study was to examine if switching from commensal to pathogenic behaviour was related to temperature. We examined the growth, motility and invasion of T84 cells by three species of Campylobacter: C. jejuni 81116, C. jejuni M1, C. coli 1669, C. coli RM2228 and C. fetus fetus NC10842 grown at 37 and 42 °C. Our results suggest that C. jejuni isolates grow similarly at both temperatures but some are more motile at 42 °C and some are more invasive at 37 °C, which may account for its rapid spread in poultry flocks and for infection in humans, respectively. C. coli, which are infrequent causes of Campylobacter infections in humans, is less able to grow and move at 37 °C compared to 42 °C but was significantly more invasive at the lower temperature. C. fetus fetus, which is infrequently found in poultry, is less able to grow and invade at 42 °C.


Assuntos
Campylobacter coli/patogenicidade , Campylobacter coli/efeitos da radiação , Campylobacter fetus/patogenicidade , Campylobacter fetus/efeitos da radiação , Campylobacter jejuni/patogenicidade , Campylobacter jejuni/efeitos da radiação , Campylobacter coli/crescimento & desenvolvimento , Campylobacter coli/fisiologia , Campylobacter fetus/crescimento & desenvolvimento , Campylobacter fetus/fisiologia , Campylobacter jejuni/crescimento & desenvolvimento , Campylobacter jejuni/fisiologia , Linhagem Celular , Endocitose , Células Epiteliais/microbiologia , Humanos , Locomoção/efeitos da radiação , Temperatura , Virulência
10.
Res Vet Sci ; 154: 113-123, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36577269

RESUMO

Wild deer products have been linked to outbreaks of Escherichia coli 0157 in humans and bovine tuberculosis (bTB) in domestic cats, raising concerns around foodborne diseases from venison. This study investigated the prevalence of several enteric disease agents in UK deer populations, including foodborne pathogens and other diseases of import to livestock health. Intestinal samples were collected from slaughtered farmed deer (N = 211, eight farms), shot wild deer (N = 136) and ground faecal samples were collected from two farms (N = 90), six parks (N = 228) and five zoos (N = 67). DNA was extracted and multiplex qPCR assays were run to amplify targets of, C. perfringens toxins, Campylobacter spp., E. coli toxins, Mycobacterium avium subsp. Paratuberculosis (MAP), Salmonella spp. and Yersinia spp. The most commonly amplified targets were E. coli astA (61.2%) and Campylobacter spp. (43.3%), but the prevalence of C. coli and C. jejuni were ≤ 3.0% and Salmonella spp., MAP and Yersinia pseudotuberculosis also had low overall prevalences of 1.6%, 3.3% and 2.6%, respectively. However, notable targets included C. perfringens toxins α (20.4%) and ß2 (16.9%); E. coli stx1 (14.6% overall, 28.0% in abattoir samples) and stx2 (17.8% overall, 31.6% in wild samples); and Yersinia enterocolitica (10.8% overall, 25% in wild samples). The low prevalence of some foodborne pathogens is reassuring for food safety; further investigations into the commonly found targets are needed to quantify the risk to public health and the possibility of wild deer acting as reservoirs of disease.


Assuntos
Cervos , Escherichia coli , Humanos , Animais , Gatos , Animais Selvagens/microbiologia , Salmonella/genética , Reino Unido/epidemiologia , Prevalência , Fezes/microbiologia
11.
Anat Rec (Hoboken) ; 306(8): 2119-2134, 2023 08.
Artigo em Inglês | MEDLINE | ID: mdl-36598271

RESUMO

There is a need for an animal model that closely parallels human cochlea gestational development. This study aims to document porcine inner ear anatomy, and in vitro porcine derived inner ear cell culture characteristics. Twenty-four temporal bone were harvested from 12 adult pigs (Sus scrofa). Six were formalin fixed and their maximal diameters were measured. The cochlea duct length was determined by the insertion length of a Nucleus 22 cochlear implant in two bones. Four formalin fixed bones were sectioned for histology. Cochlear and vestibular tissues were harvested from non-fixed bones, cultured and characterized at different passages (P). Gene and protein expression of multipotent stem/progenitor (Nestin and Sox2), inner ear hair (Myosin VIIa, Prestin) and supporting (Cytokeratin 18 and Vimentin) cell markers were determined. The porcine cochlea was a 3.5 turn spiral. There was a separate vestibular compartment. The cochlear mean maximal diameter and height was 7.99 and 3.77 mm, respectively. Sphere forming cells were identified on phase-contrast microscopy. The relative mRNA expression levels of KRT18, MYO7A and SLC26A5 were significantly positively correlated in cochlear cultures; and MYO7A and SLC26A5; SOX2 and KRT18; NES and SLC26A5 genes were positively correlated in vestibular cultures (p = .037, Spearman correlation [τ] = .900). Inner ear sensory and stem cell characteristics persist in passaged porcine inner ear cells. Further work is required to establish the usefulness of porcine inner ear cell cultures to the study of human inner ear disorders.


Assuntos
Implante Coclear , Implantes Cocleares , Vestíbulo do Labirinto , Animais , Adulto , Humanos , Suínos , Cóclea , Sus scrofa
12.
One Health ; 14: 100370, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35146110

RESUMO

We report a survey (August 2017 to March 2018) and risk factor analysis of faecal carriage of antibacterial-resistant (ABR) Escherichia coli in 223 16-week-old dogs in the United Kingdom. Raw feeding was associated with the presence of fluoroquinolone-resistant (FQ-R) E. coli and those resistant to tetracycline, amoxicillin, and streptomycin, but not to cefalexin. Whole genome sequencing of 36 FQ-R E. coli isolates showed a wide range of sequence types (STs), with almost exclusively mutational FQ-R dominated by ST744 and ST162. Comparisons between E. coli isolates from puppies known to be located within a 50 × 50 km region with those isolated from human urinary tract infections (isolated in parallel in the same region) identified an ST744 FQ-R lineage that was carried by one puppy and caused one urinary tract infection. Accordingly, we conclude that raw feeding is associated with carriage of ABR E. coli in dogs even at 16 weeks of age and that bacteria carried by puppies are shared with humans. We therefore suggest that those who feed their dogs raw meat seriously consider the potential ABR-transmission threat their pet may become as a result and deploy appropriate hygiene practices in mitigation.

13.
Viruses ; 14(9)2022 08 24.
Artigo em Inglês | MEDLINE | ID: mdl-36146663

RESUMO

Respiratory pathogens can be spread though the transmission of aerosolised expiratory secretions in the form of droplets or particulates. Understanding the fundamental aerosol parameters that govern how such pathogens survive whilst airborne is essential to understanding and developing methods of restricting their dissemination. Pathogen viability measurements made using Controlled Electrodynamic Levitation and Extraction of Bioaerosol onto Substrate (CELEBS) in tandem with a comparative kinetics electrodynamic balance (CKEDB) measurements allow for a direct comparison between viral viability and evaporation kinetics of the aerosol with a time resolution of seconds. Here, we report the airborne survival of mouse hepatitis virus (MHV) and determine a comparable loss of infectivity in the aerosol phase to our previous observations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Through the addition of clinically relevant concentrations of mucin to the bioaerosol, there is a transient mitigation of the loss of viral infectivity at 40% RH. Increased concentrations of mucin promoted heterogenous phase change during aerosol evaporation, characterised as the formation of inclusions within the host droplet. This research demonstrates the role of mucus in the aerosol phase and its influence on short-term airborne viral stability.


Assuntos
COVID-19 , SARS-CoV-2 , Animais , Camundongos , Viabilidade Microbiana , Mucinas , Aerossóis e Gotículas Respiratórios
14.
Lancet ; 372(9655): 2023-30, 2008 12 13.
Artigo em Inglês | MEDLINE | ID: mdl-19022496

RESUMO

BACKGROUND: The loss of a normal airway is devastating. Attempts to replace large airways have met with serious problems. Prerequisites for a tissue-engineered replacement are a suitable matrix, cells, ideal mechanical properties, and the absence of antigenicity. We aimed to bioengineer tubular tracheal matrices, using a tissue-engineering protocol, and to assess the application of this technology in a patient with end-stage airway disease. METHODS: We removed cells and MHC antigens from a human donor trachea, which was then readily colonised by epithelial cells and mesenchymal stem-cell-derived chondrocytes that had been cultured from cells taken from the recipient (a 30-year old woman with end-stage bronchomalacia). This graft was then used to replace the recipient's left main bronchus. FINDINGS: The graft immediately provided the recipient with a functional airway, improved her quality of life, and had a normal appearance and mechanical properties at 4 months. The patient had no anti-donor antibodies and was not on immunosuppressive drugs. INTERPRETATION: The results show that we can produce a cellular, tissue-engineered airway with mechanical properties that allow normal functioning, and which is free from the risks of rejection. The findings suggest that autologous cells combined with appropriate biomaterials might provide successful treatment for patients with serious clinical disorders.


Assuntos
Broncomalácia/fisiopatologia , Condrócitos/citologia , Engenharia Tecidual/métodos , Traqueia/transplante , Adulto , Broncomalácia/terapia , Cadáver , Feminino , Humanos , Período Pós-Operatório , Testes de Função Respiratória , Traqueia/citologia
15.
Toxins (Basel) ; 11(10)2019 09 20.
Artigo em Inglês | MEDLINE | ID: mdl-31547149

RESUMO

Bovine mycotoxicosis is a disorder caused by the ingestion of fungal toxins. It is associated with chronic signs, such as reduced growth rate and milk yield, and causes significant economic cost to the dairy industry. The mycotoxins deoxynivalenol (DON), zearalenone (ZEN), and fumonisin B1 (FB1) are commonly found in grain fed to cattle. Patulin (PA) is a common grass silage contaminant but is also found in grain. The effects of these mycotoxins on cellular function at low concentrations are not well understood. Using Madin-Darby bovine kidney cells we evaluated the cellular response to these mycotoxins, measuring cytotoxicity, de novo protein synthesis, cell proliferation, cell cycle analysis, and also metabolic profiling by 1H NMR spectroscopy. DON, ZEN, and PA induced cytotoxicity, and PA and FB1 induced a decrease in metabolic activity in surviving cells. DON was the only mycotoxin found to have a significant effect on the metabolic profile, with exposed cells showing increased cellular amino acids, lactate, 2-oxoglutarate, 3-hydroxybutyrate, and UDP-N-acetylglucosamine and decreased ß-alanine, choline, creatine, taurine, and myo-inositol. Cells exposed to DON also showed reductions in protein synthesis. DON has previously been documented as being a ribotoxin; the results here suggest that exposure of bovine cells to DON causes a decrease in protein synthesis with corresponding cellular accumulation of precursors. Cell proliferation was also arrested without causing apoptosis. It is likely that exposure triggers hypoxic, hypertonic, and ribotoxic responses in bovine cells, and that these responses contribute to reduced productivity in exposed cattle.


Assuntos
Rim/efeitos dos fármacos , Tricotecenos/toxicidade , Animais , Bovinos , Ciclo Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/fisiologia , Rim/fisiologia , Biossíntese de Proteínas/efeitos dos fármacos
16.
Microb Genom ; 5(7)2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31310201

RESUMO

Reference and type strains of well-known bacteria have been a cornerstone of microbiology research for decades. The sharing of well-characterized isolates among laboratories has run in parallel with research efforts and enhanced the reproducibility of experiments, leading to a wealth of knowledge about trait variation in different species and the underlying genetics. Campylobacter jejuni strain NCTC 11168, deposited at the National Collection of Type Cultures in 1977, has been adopted widely as a reference strain by researchers worldwide and was the first Campylobacter for which the complete genome was published (in 2000). In this study, we collected 23 C. jejuni NCTC 11168 reference isolates from laboratories across the UK and compared variation in simple laboratory phenotypes with genetic variation in sequenced genomes. Putatively identical isolates, identified previously to have aberrant phenotypes, varied by up to 281 SNPs (in 15 genes) compared to the most recent reference strain. Isolates also display considerable phenotype variation in motility, morphology, growth at 37 °C, invasion of chicken and human cell lines, and susceptibility to ampicillin. This study provides evidence of ongoing evolutionary change among C. jejuni isolates as they are cultured in different laboratories and highlights the need for careful consideration of genetic variation within laboratory reference strains. This article contains data hosted by Microreact.


Assuntos
Campylobacter jejuni/genética , Campylobacter jejuni/isolamento & purificação , Variação Genética , Genoma Bacteriano , DNA Bacteriano/genética , Fenótipo
17.
Inflamm Bowel Dis ; 14(3): 303-9, 2008 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-18050297

RESUMO

BACKGROUND: The intestinal epithelium is a single layer of polarized cells and is the primary barrier separating foreign antigen and underlying lymphoid tissue. IFNgamma alters epithelial barrier function during inflammation by disrupting tight cell junctions and facilitating the paracellular transport of luminal antigens. The aim of this work was to determine whether Campylobacter infection of cells exposed to IFNgamma would lead to greater disruption of cell monolayers and hence increased bacterial translocation. METHODS: Monolayers were polarized on Transwell polycarbonate membranes for 14 days and then cultured in the presence or absence of 100 U/mL IFNgamma. Campylobacter was added to the apical side of the monolayer at an MOI of 30. Transepithelial electrical resistance (TEER) was recorded and bacteria in the basal well counted every 2 hours. Cells were stained for occludin, actin, and nuclear DNA, and cell viability determined by measurement of apoptosis. RESULTS: In the presence of IFNgamma, TEER dropped significantly after 18 hours, indicating a reduction in barrier function. A further significant decrease was seen in the presence of both IFNgamma and Campylobacter, indicating a synergistic effect, and cellular morphology and viability were affected. Bacterial translocation across the monolayer was also significantly greater in the presence of IFNgamma. CONCLUSIONS: These combined effects indicate that Campylobacter infection concomitant with intestinal inflammation would result in a rapid and dramatic loss of epithelial barrier integrity, which may be a key event in the pathogenesis of Campylobacter-mediated colitis and the development of bloody diarrhea.


Assuntos
Translocação Bacteriana/fisiologia , Campylobacter jejuni/fisiologia , Permeabilidade da Membrana Celular/efeitos dos fármacos , Interferon gama/farmacocinética , Mucosa Intestinal/metabolismo , Actinas/metabolismo , Apoptose/efeitos dos fármacos , Apoptose/fisiologia , Translocação Bacteriana/efeitos dos fármacos , Células CACO-2 , Infecções por Campylobacter/metabolismo , Infecções por Campylobacter/microbiologia , Infecções por Campylobacter/patologia , Epitélio/metabolismo , Epitélio/microbiologia , Epitélio/patologia , Humanos , Mucosa Intestinal/microbiologia , Mucosa Intestinal/patologia , Potenciais da Membrana/efeitos dos fármacos , Proteínas de Membrana/metabolismo , Microscopia de Fluorescência , Ocludina
18.
FEMS Microbiol Lett ; 281(2): 155-9, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18312578

RESUMO

The ability to survive desiccation between hosts is often essential to the success of pathogenic bacteria. The bacterial outer membrane is both the cellular interface with hostile environments and the focus of much of the drying-induced damage. This study examined the contribution of outer membrane-associated polysaccharides to the survival of Salmonella enterica serovar Typhimurium in air-dried blood droplets following growth in high and low osmolarity medium and under conditions known to induce expression of these polysaccharides. Strains lacking the O polysaccharide (OPS) element of the outer membrane lipopolysaccharide were more sensitive to desiccation. Lipopolysaccharide core mutation further to OPS loss did not result in increased susceptibility to drying. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed lipopolysaccharide profiles that supported the hypothesis that OPS expression is required for optimal drying resistance in S. Typhimurium. The role of O antigen in Salmonella spp. in maintaining a hydrated layer around the dried cell or in slowing the rate of dehydration and rehydration is discussed.


Assuntos
Proteínas da Membrana Bacteriana Externa/metabolismo , Dessecação , Lipopolissacarídeos/metabolismo , Salmonella typhimurium/fisiologia , Proteínas da Membrana Bacteriana Externa/química , Proteínas da Membrana Bacteriana Externa/genética , Eletroforese em Gel de Poliacrilamida , Lipopolissacarídeos/análise , Antígenos O/análise , Antígenos O/genética , Antígenos O/metabolismo , Salmonella typhimurium/química , Salmonella typhimurium/genética , Salmonella typhimurium/crescimento & desenvolvimento
19.
Res Microbiol ; 158(1): 79-85, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-17227702

RESUMO

Polyphosphate is involved in resistance to stress in a number of bacterial species; however, its role in the virulence of Salmonella enterica serovars which differ in their host range has not been described. We examined the role of polyphosphate kinase in infection, growth and survival of S. Typhimurium (broad-host range) and S. Gallinarum (avian-adapted). We also used ppk mutants to assess the downstream effects on intracellular ATP levels. ppk mutants had significantly (P<0.05) elevated ATP in stationary phase compared to the wild-type and, depending on the serovar, were defective in growth, survival and virulence. The virulence of S. Typhimurium ppk::SpcStr was significantly (P<0.05) attenuated following oral infection of both Rhode Island Red chickens and BALB/c mice. In contrast, inactivation of the ppk gene of S. Gallinarum did not affect growth or virulence. The differential contribution of polyphosphate to the virulence of S. Typhimurium and S. Gallinarum may reflect aspects of the pathogenesis and host range of these serovars. The ppk mutant of both serovars survived significantly less well (P<0.05) in a saline starvation-survival model, relative to the respective parent. The effect of ppk mutation on survival was formally described by fitting the data to the Weibull model and by estimation of k(max). Measurement of rpoS promoter activity using a lacZ transcriptional fusion demonstrated repression of rpoS in a ppk background, confirming a role for polyphosphate in RpoS induction. Together the data indicate the crucial importance of maintaining stable intracellular ATP during infection and nutritional stress. We suggest that polyphosphate plays a central role in homeostasis during growth and stress.


Assuntos
Trifosfato de Adenosina/metabolismo , Fosfotransferases (Aceptor do Grupo Fosfato)/fisiologia , Infecções por Salmonella/microbiologia , Salmonella enterica/fisiologia , Salmonella enterica/patogenicidade , Animais , Galinhas , Genes Bacterianos , Camundongos , Camundongos Endogâmicos BALB C , Mutação Puntual , Salmonella typhimurium/patogenicidade , Salmonella typhimurium/fisiologia , Especificidade da Espécie , Virulência
20.
Nat Genet ; 48(10): 1211-1217, 2016 10.
Artigo em Inglês | MEDLINE | ID: mdl-27548315

RESUMO

An epidemiological paradox surrounds Salmonella enterica serovar Enteritidis. In high-income settings, it has been responsible for an epidemic of poultry-associated, self-limiting enterocolitis, whereas in sub-Saharan Africa it is a major cause of invasive nontyphoidal Salmonella disease, associated with high case fatality. By whole-genome sequence analysis of 675 isolates of S. Enteritidis from 45 countries, we show the existence of a global epidemic clade and two new clades of S. Enteritidis that are geographically restricted to distinct regions of Africa. The African isolates display genomic degradation, a novel prophage repertoire, and an expanded multidrug resistance plasmid. S. Enteritidis is a further example of a Salmonella serotype that displays niche plasticity, with distinct clades that enable it to become a prominent cause of gastroenteritis in association with the industrial production of eggs and of multidrug-resistant, bloodstream-invasive infection in Africa.


Assuntos
Enterocolite/microbiologia , Infecções por Salmonella/microbiologia , Salmonella enteritidis , Adaptação Biológica , África Subsaariana/epidemiologia , Animais , Galinhas/microbiologia , Enterocolite/epidemiologia , Enterocolite/veterinária , Epidemias/economia , Feminino , Genoma Bacteriano , Humanos , Renda , Plasmídeos , Doenças das Aves Domésticas/microbiologia , Infecções por Salmonella/economia , Infecções por Salmonella/epidemiologia , Infecções por Salmonella/transmissão , Salmonella enteritidis/classificação , Salmonella enteritidis/patogenicidade , Análise de Sequência de DNA
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