RESUMO
The genetic variability of Entamoeba dispar strains was investigated in 39 positive isolates on a survey of 1783 individuals from two different cities of Northeast Brazil (Recife and Macaparana) using two polymorphic species-specific loci (loci 1-2 and 5-6). A combinatory clustering analysis revealed no geographical correlation and remarkable genetic polymorphism among all the isolates examined. Nevertheless, a comparison of the frequency of eight individual PCR products, shared by both Recife and Macaparana populations, for the two loci, showed that only one product of locus 5-6 was significantly different between the two cities. These results suggested that the Macaparana population is infected by similar strains and that locus 5-6 shows potential in assaying questions related to the molecular epidemiology of this region.
Assuntos
Disenteria Amebiana/parasitologia , Entamoeba/genética , Enteropatias Parasitárias/parasitologia , Adolescente , Animais , Brasil/epidemiologia , Criança , Pré-Escolar , Análise por Conglomerados , DNA de Protozoário/química , DNA de Protozoário/genética , Disenteria Amebiana/epidemiologia , Entamoeba/isolamento & purificação , Fezes/parasitologia , Variação Genética , Humanos , Enteropatias Parasitárias/epidemiologia , Reação em Cadeia da Polimerase , Áreas de Pobreza , População Rural , População UrbanaRESUMO
Previous studies using methods varying from traditional serologic tests to molecular biology techniques have shown that in northeastern Brazil, Entamoeba dispar was more prevalent than E. histolytica. In this study, the prevalence was established by using E. histolytica stool antigen detection kits and a polymerase chain reaction (PCR) with genomic DNA extracted from cultured trophozoites in all four-nuclei, amoeba-positive samples from a population living in Macaparana in northeastern Brazil. Among 1,437 stool samples analyzed, only 59 (4.1%) were positive for four nuclei amoeba. However, all of these samples were negative in an immunoenzymatic assay for the presence of E. histolytica-specific galactose adhesin. Of 59 cultivated samples, only 31 showed trophozoites. Extraction of DNA from these 31 samples, followed by the PCR, showed that 23 samples (74.19%) were positive for E. dispar and no amplification was observed for pathogenic E. histolytica. The remaining eight samples were negative for both species. These findings are consistent with those previously reported.
Assuntos
DNA de Protozoário/análise , Entamoeba histolytica/isolamento & purificação , Entamoeba/isolamento & purificação , Entamebíase/epidemiologia , Fezes/parasitologia , Animais , Antígenos de Protozoários/análise , Brasil/epidemiologia , Entamoeba/genética , Entamoeba/imunologia , Entamoeba histolytica/genética , Entamoeba histolytica/imunologia , Entamebíase/parasitologia , Humanos , Reação em Cadeia da Polimerase , PrevalênciaRESUMO
This article documents the addition of 153 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Brassica oleracea, Brycon amazonicus, Dimorphandra wilsonii, Eupallasella percnurus, Helleborus foetidus, Ipomoea purpurea, Phrynops geoffroanus, Prochilodus argenteus, Pyura sp., Sylvia atricapilla, Teratosphaeria suttonii, Trialeurodes vaporariorum and Trypanosoma brucei. These loci were cross-tested on the following species: Dimorphandra coccicinea, Dimorphandra cuprea, Dimorphandra gardneriana, Dimorphandra jorgei, Dimorphandra macrostachya, Dimorphandra mollis, Dimorphandra parviflora and Dimorphandra pennigera.