RESUMO
A multicenter evaluation was undertaken to evaluate the performance of a new three-color peptide nucleic acid fluorescence in situ hybridization assay that identifies isolates directly from blood cultures positive for Gram-negative bacilli (GNB). The assay correctly identified 100% (186/186) of the Escherichia coli isolates, 99.1% (109/110) of the Klebsiella pneumoniae isolates, and 95.8% (46/48) of the Pseudomonas aeruginosa isolates in this study. Negative assay results were correctly obtained for 162 of 165 other GNB (specificity, 98.2%).
Assuntos
Técnicas Bacteriológicas/métodos , Sangue/microbiologia , Escherichia coli/isolamento & purificação , Hibridização in Situ Fluorescente/métodos , Klebsiella pneumoniae/isolamento & purificação , Ácidos Nucleicos Peptídicos , Pseudomonas aeruginosa/isolamento & purificação , Cor , Humanos , Sensibilidade e EspecificidadeRESUMO
A shortened protocol for two peptide nucleic acid fluorescence in situ hybridization (PNA FISH) assays for the detection of Gram-negative bacilli from positive blood cultures was evaluated in a multicenter trial. There was 100% concordance between the two protocols for each assay (368 of 368 and 370 of 370 results) and 99.7% (367 of 368 and 369 of 370 results) agreement with routine laboratory techniques.