[Preliminary survey on the host of Angiostrongylus cantonensis in three plateau lakes of Yunnan Province].

OBJECTIVE: To investigated the intermediate hosts of Angiostrongylus cantonensis in three plateau lakes of Yunnan Province, and analyze the effect of temperature on A. cantonensis during 1991-2010. METHODS: An epidemiological investigation of angiostrongyliasis cantonensis in Erhai Lake, Fuxian Lake and Xingyun Lake was conducted from April to September in 2012. Snails were examined for the third stage larvae by enzyme digestion or lung examination. Rodents were captured in the fields, and their hearts and lungs were dissected for adult worms. The potential distribution of A. cantonensis and its main intermediate host Pomacea canaliculata were predicted based on degree-day models using GIS technique. RESULTS: A total of 4 950 snails were collected, belonging to 4 species, P. canaliculata, Cipangopaludina chinensis, Bellamya aeruginosa, and B. quadrata. 174 rodents were captured, belonging to 5 species. No positive samples were found. The potential distribution map showed that the distribution of A. cantonensis and P. canaliculata in Yunnan would expand with the rise of temperature, and with the passage of time they could complete one generation in the region which couldn't finish one generation in one year along with time passing. CONCLUSION: A. cantonensis are not found in the hosts. The natural environment and ecological system of the three lakes match the condition of A. cantonensis transmission.

[Application of PCR and PCR-derived technologies in the detection of Angiostrongylus cantonensis].

Angiostrongyliasis is a zoonotic disease and has become one of the potentially threatening food-borne parasitic infections in China. This article reviews the advances in the application of PCR and PCR-derived techniques in detection of Angiostrongylus cantonensis.

[Application of immune colloidal gold technique on the diagnosis of parasitoses].

Immune colloidal gold (ICG) technique is a simple, rapid, accurate diagnosis method. At present, ICG technique has been widely applied in the accessory diagnosis of parasitoses, and this article reviews the latest progress of ICG technique applied in parasitology.