RESUMO
The altered expression levels of S100 proteins can lead to four different categories of diseases: diseases of the heart and of the central nervous system, inflammatory disorders and cancer. Various studies have shown the lack of harmonization of the results obtained with different methods, mainly due to different performances and measurements of S100B. The purpose of this work was to compare quantitatively the fully automated Elecsys® immunoassay with the reference immunoenzimatic method CanAg® EIA for serum S100B protein. In the study serum samples were analyzed of 161 patients: 85 females (aged 22-83 years) and 76 males (aged 16-90 years), affected by oncological and non-oncological pathologies. PassingBablok regression was used to analyze the comparison between the assays; it showed a strong interassay correlation: r = 0.9350 (95% CI =0.9122 0.9520), with an intercept of 0.02063 (95% CI=-0.02850 0.01400) and a slope of 1.1125 (95% CI=1.0200 1.2417). Elecsys® S100 assay should be preferred to CanAg® S100 for better standardization, good reliability and precision but also with the aim to reduce costs and obtain results in a shorter time.
Assuntos
Biomarcadores/sangue , Ensaio de Imunoadsorção Enzimática/métodos , Medições Luminescentes/métodos , Subunidade beta da Proteína Ligante de Cálcio S100/sangue , Adulto , Idoso , Idoso de 80 Anos ou mais , Técnicas Eletroquímicas/métodos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto JovemRESUMO
Various methods have been investigated to concentrate hemopoietic stem cells before in vitro treatment and cryopreservation. Each procedure requires various degrees of cell manipulation; moreover, the extent of purification of the stem cell fraction depends on the further use of bone marrow. Fifty four bone marrows were treated with hydroxyethyl starch (HES) to isolate the mononuclear cell population. The manipulation with HES permitted to concentrate marrow stem cells in a small volume with removal of unwanted granulocytes, red blood cells and the donor isohemagglutinins in the major ABH incompatibility. We describe here a manual method which permits the transfusion of a very few number of red cells without depletion of progenitor cells and thus delay of engraftment.
Assuntos
Células da Medula Óssea , Transplante de Medula Óssea/métodos , Separação Celular/métodos , Células-Tronco Hematopoéticas , Derivados de Hidroxietil Amido , Leucócitos Mononucleares , Incompatibilidade de Grupos Sanguíneos , Sedimentação Sanguínea/efeitos dos fármacos , Medula Óssea/patologia , Contagem de Células , Hemaglutininas , Transplante de Células-Tronco Hematopoéticas , Humanos , Derivados de Hidroxietil Amido/farmacologia , Isoanticorpos , Leucócitos Mononucleares/transplante , Transplante Autólogo , Transplante HomólogoRESUMO
In S. Camillo Hospital, Rome, the Apheresis Center (CNTS-CRI) and the Haematological Division, Authors treated 17 patients affected by haematologic malignances and solid tumours with leukoapheresis procedures for PBSC collection. Nine patients were treated with continuous-flow cell separator Dideco Vivacell, performing a total of 50 collection procedures. Mean values of total collected NC and MNC were 6.5 x 10(9) and 5.5 x 10(9), with a mean recovery 24% and 52%. Authors took patient's blood samples during the procedure, in order to analyze the MNC collection and to optimize the separation efficiency. Haemopoietic reconstitution values of the eight patients submitted to APBSCT showed the effectiveness of our method of cell collection.