Modulation of Apoptosis by Bovine Gammaherpesvirus 4 Infection in Bovine Endometrial Cells and the Possible Role of LPS in This Process.

The prevalent pathogens associated with bovine uterine infections are bacteria that appear to increase the host's susceptibility to secondary infections with other bacteria or viruses, among which BoGHV4 is the most frequently found. In this work, the study of the pathways of apoptosis induction was carried out on an experimental model of primary culture of endometrial cells, in order to know the implication of BoGHV4 and the presence of bacterial LPS in the pathogenesis of the bovine reproductive tract. For this, different staining techniques and molecular analysis by RT-PCR were used. The results obtained allowed us to conclude that the level of cell death observed in the proposed primary culture is directly related to the time of viral infection and the presence of LPS in BoGHV4 infection. The apoptosis indices in cells infected with BoGHV4 and BoGHV4 + LPS revealed a maximum that correlated with the appearance of cytopathic effects and the maximum viral titers in the model studied. However, morphological, biochemical, and molecular changes were evident during both early and late stages of apoptosis. These findings provide information on the factors that may influence the pathogenesis of BoGHV4 and help to better understand the mechanisms involved in virus infection.

Acardia fetal en un embarazo triple / Fetal acardia in triple pregnancy

Se presenta el caso de una paciente de 24 años que ingresa al Hospital Regional de Lambayeque con una gestación doble bicorial biamniótica de 34 semanas 4 días por ecografía de II trimestre, en cuya ecografía al ingreso se evidenciaba una imagen dependiente de placenta probable teratoma placentario. Es hospitalizada con diagnósticos: Amenaza de parto pretérmino, feto 2 en transverso. Habiendo recibido tocólisis y maduración pulmonar se culmina la gestación por cesárea por parto pretérmino, obteniéndose dos recién nacidos de adecuado peso para la edad gestacional con dos placentas y una masa sólida de tejido embrionario con cordón procedente de una de las placentas, cuya anatomía patológica concluye: feto acárdico amorfo, producto de una perfusión feto fetal.