RESUMO
The diagnosis of neurosyphilis (NS) is a challenge, especially in HIV-infected patients, and the criteria for deciding when to perform a lumbar puncture (LP) in HIV-infected patients with syphilis are controversial. We retrospectively reviewed demographic, clinical, and laboratory data from 122 cases of HIV-infected patients with documented early syphilis who underwent an LP to rule out NS, and we evaluated 3 laboratory-developed validated real-time PCR assays, the Treponema pallidum particle agglutination (TPPA) assay, the fluorescent treponemal antibody absorption (FTA-ABS) assay, and the line immunoassay INNO-LIA Syphilis, for the diagnosis of NS from cerebrospinal fluid (CSF) samples of these patients. NS was defined by a reactive CSF-VDRL test result and/or a CSF white blood cell (WBC) count of >20 cells/µl. Thirty of the 122 patients (24.6%) had early NS. Headache, visual symptoms, a CD4 cell count of <500 cells/µl, and viremia, as defined by an HIV-1 RNA count of ≥50 copies/ml, were associated with NS in multivariate analysis (P = <0.001 for each factor). Blood serum rapid plasma reagin (RPR) titers were not associated with early NS (P = 0.575). For the diagnosis of NS, the PCR, FTA-ABS, TPPA, and INNO-LIA assays had sensitivities of 58%, 100%, 68%, and 100%, specificities of 67%, 12%, 49%, and 13%, and negative predictive values of 85%, 100%, 84%, and 100%, respectively. Visual disturbances, headache, uncontrolled HIV-1 viremia, and a CD4 cell count of <500 cells/µl were predictors of NS in HIV-infected patients with early syphilis, while blood serum RPR titers were not; therefore, RPR titers should not be used as the sole criterion for deciding whether to perform an LP in early syphilis. When applied to CSF samples, the INNO-LIA Syphilis assay easily helped rule out NS.
Assuntos
Anticorpos Antibacterianos/análise , Líquido Cefalorraquidiano/microbiologia , Técnicas de Laboratório Clínico/métodos , Infecções por HIV/complicações , Neurossífilis/diagnóstico , Neurossífilis/patologia , Treponema pallidum/isolamento & purificação , Adulto , Idoso , Feminino , HIV-1/isolamento & purificação , Humanos , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Prognóstico , Estudos Retrospectivos , Sensibilidade e Especificidade , Treponema pallidum/imunologia , Adulto JovemRESUMO
This article provides a road map, along with recommendations, for the adoption and implementation of telesimulation at a large scale. We provide tools for translating an in-presence simulation curriculum into a telesimulation curriculum using a combination off-the-shelf telecommunication platform. We also describe the roles and tasks that emerged within the simulation team when planning and delivering a telesimulation curriculum.
RESUMO
BACKGROUND: Although simulation-based teaching is popular, high-fidelity, high-cost approaches may be unsuitable or unavailable for use with large groups. We designed a multiple-choice test for large groups of medical students to explore a low-cost approach in assessing clinical competence. We tested two different scenarios in assessing student's ability to identify heart and lung sounds: by hearing the sounds alone, or in an enhanced scenario where sounds are incorporated into clinical vignettes to give clinical context. METHOD: The two-section test consists of multiple-choice questions with one best answer. In the first section, the student must identify 25 auscultation sounds from amongst a choice of 14 heart sounds and 11 lung-sounds. The second section integrates these same sounds into clinical vignettes to provide clinical context. Students must either identify the illness or the next clinical step, choosing from four possible answers. Performances of 859 students were evaluated. RESULTS: The alpha coefficient of reliability is 0.54 and 0.76 respectively for the first and the second section. In the latter section there is significant difference between scores of first, second, fourth year students and residents, in contrast to the first-section scores. CONCLUSIONS: A multiple-choice test to assess clinical competence based on simulated auscultation sounds incorporated into clinical vignettes allows us to differentiate between training levels and seems to be a valid assessment method suitable for large-group format.