[Clinical study on application of 3D Slicer software assisted domestic frameless stereotactic robot in biopsy of intracranial lesions].

Objective: To examine the application value of 3D Slicer software assisted domestic frameless stereotactic robot in biopsy of intracranial lesions. Methods: A retrospective analysis was performed on 80 patients who admitted consecutively and underwent intracerebral lesions biopsy with the domestic frameless stereotactic robot at Department of Neurosurgery, Aerospace Central Hospital from January 2019 to December 2021. There were 36 males and 44 females, with a mean age of (38.5±18.0) years (range: 6 to 71 years). Before surgery only enhanced T1-weighted three-dimensional magnetization prepared gradient echo sequences and diffusion tensor imaging scans were performed. Self-reconstruction of intracranial lesions, cerebral cortex and blood vessels was carried out using 3D Slicer software system after the DICOM format imaging data of 80 patients were collected. These imaging data were merged to the workstation of the domestic frameless stereotactic robot for preoperative surgical planning and the surgical puncture path was designed to avoid blood vessels in the brain functional area, cerebral cortex and sulcus. Results: All frameless stereotactic biopsy were successfully performed. Postoperative pathological diagnosis included 50 cases of diffuse astrocytic and oligodendroglioma, 15 cases of lymphoma, 5 cases of metastatic tumors, 5 cases of inflammatory demyelinating disease, 2 cases of inflammatory granuloma, 1 case of hemangioma, 1 case of acute lymphoblastic leukemia intracranial invasion and 1 case of seminoma. The positive diagnosis rate was 100% (80/80). Postoperative imaging confirmed that the puncture path and target were accurately implemented according to the preoperative planning, and the target error was (1.32±0.44) mm (range: 0.55 to 1.99 mm). One case of puncture-related bleeding occurred at the target after surgery and improved after treatment. Conclusion: The three-dimensional multimodal images reconstructed by the 3D Slicer software before operation could help the surgeons make the preoperative planning and reduce the risk of stereotactic brain biopsy.

[PrepFiler Express BTATM Lysis Buffer Combined with Silicon Microbeads for Rapid DNA Extraction from Bone].

OBJECTIVES: To establish a convenient and rapid method for extracting DNA from bone. METHODS: Fifteen long bone samples were washed and sterilized. The skeletal fragments were obtained by electric drill, and lysed by PrepFiler Express BTA™ lysis buffer. DNA was then manually extracted by silicon microbeads for further analysis. RESULTS: STR genotyping was successfully obtained in 14 out of the 15 samples, and the detection rate was 93.33%. CONCLUSIONS: The method for DNA extraction from bone established in present study is convenient, quick, effective, and with a strong applicability, which is worth spreading and applying.

[Clinical and experimental study on fuganlin oral liquid in treating repeated respiratory tract infection].

OBJECTIVE: To assess the efficacy of Fuganlin (FGL) oral liquid, a pure Chinese medicinal recipe in treating the repeated infantile respiratory tract infection. METHODS: FGL was used for repeated infantile respiratory tract infections. The children who took the antipyretic oral liquid as the control. Before and after treatment, immune function and other test were determined in both groups. Wistar rats taking FGL for three months was also observed. RESULTS: The curative and marked effective rates of the treated group were 76.8%, and the curative and marked effective rates of the control group were 48.0%. There are significant differences between the two groups (P < 0.01). CONCLUSIONS: FGL could not only modulate immune function but also could serve as a tonic as well as symptomatic relief for fever, cough, asthma, anti-viral, anti-bacterial infection and anti-inflammatory function.