RESUMO
Porcine circovirus type 3 (PCV3) was first detected in 2016 and has been reported in many pig-producing countries around the world, including Vietnam. PCV3 has been found in complex cases with multiple clinical syndromes in swine. In this study, we investigated the genetic diversity of PCV3 strains circulating in Vietnam. A total of 249 samples were collected from swine farms located in eight provinces of Vietnam, and 11.65% (29/249) of these samples were found to contain PCV3. The ORF2 genes from the 29 PCV3-positive samples were amplified, purified, and sequenced. Phylogenetic analysis showed that 23 of these strains belonged to the PCV3b subtype, while the remaining six strains belonged to subtype c and subtype a (a-1 and a-2). Analysis of the ORF2 genes indicated that the 29 PCV3 strains had high sequence identity (96.90-100% at the genomic level and 96.19-100% at the amino acid level). Fifteen amino acid substitutions were found in predicted B-cell epitopes in the capsid proteins of the Vietnamese PCV3 strains.
Assuntos
Infecções por Circoviridae , Circovirus , Doenças dos Suínos , Suínos , Animais , Proteínas do Capsídeo/genética , Circovirus/genética , Filogenia , Vietnã/epidemiologia , Infecções por Circoviridae/veterinária , Doenças dos Suínos/epidemiologia , Variação GenéticaRESUMO
African swine fever (ASF) is a dangerous infectious disease of domestic pigs and wild boar caused by African swine fever virus (ASFV). In Vietnam, the ASF epidemic is gradually turning into an endemic status with several recovered pigs post infection, but there were not many studies evaluating the role of these pigs in the epidemiological context in Vietnam. The aim of this study was to evaluate the viral antigen distribution and lesions in recovered pigs post ASFV infection. Ten pigs recovered from ASF at 6 weeks of age were monitored and assessed for anti-ASFV antibodies and viremia until slaughter. The five major organs (lung, liver, spleen, kidney, and lymph nodes) of these pigs were evaluated for microscopic lesions and viral antigen distribution. Anti-ASFV antibody was consistently observed to be high (S/P% ≥ 80) until slaughter, while viremia levels were very high (7 log10 copies/mL) at 6 weeks of age and gradually decreased to undetectable levels at 12 weeks of age (6th week post-infection). At slaughter, the ASFV-associated lesions in the organs of these pigs were mild and nonspecific. Seven out of ten pigs recovering from ASF still carried the virus in surveyed organ tissues, although not in the serum. These findings suggest that ASF-recovered pigs may be potential carriers of the virus, contributing to the increased complexity in the current endemic status in Vietnam.
Assuntos
Vírus da Febre Suína Africana , Febre Suína Africana , Doenças dos Suínos , Febre Suína Africana/epidemiologia , Animais , Antígenos Virais , Sus scrofa , Suínos , Viremia/veterináriaRESUMO
This study was conducted to investigate the genetic diversity of porcine circovirus type 2 (PCV2) and its coinfecting pathogens in pigs with respiratory disease in Vietnam. Samples from 127 clinical cases were obtained from different southern provinces of Vietnam from January 2018 to January 2020 for PCR and sequence analysis. The infection rate of PCV2 was 78.8%, and the major pathogens found in coinfections with PCV2 were porcine reproductive and respiratory syndrome virus, Mycoplasma hyopneumoniae, and Haemophilus parasuis. Forty-three PCV2-positive clinical samples were selected for amplification and sequencing of the ORF2 region. Phylogenetic analysis of PCV2 ORF2 showed that five of the sequences belonged to PCV2b (11.6%) and 38 belonged to PCV2d (88.4%), indicating that PCV2d strains were predominant in southern provinces of Vietnam. Alignment of the predicted amino acid sequences of the PCV2 capsid protein revealed polymorphic sites in the antibody recognition regions. This study demonstrates the prevalence of the PCV2d genotype in southern Vietnam and presents a comprehensive overview of the coinfecting pathogens associated with PCV2 in young pigs with respiratory disease.
Assuntos
Infecções por Circoviridae/virologia , Circovirus/genética , Coinfecção/virologia , Doenças Respiratórias/virologia , Doenças dos Suínos/virologia , Sequência de Aminoácidos , Animais , Proteínas do Capsídeo/genética , Genótipo , Prevalência , Suínos , VietnãRESUMO
Porcine circovirus type 3 (PCV3) is an emerging circovirus that is highly distributed among swine worldwide and associated with porcine dermatitis and nephropathy syndrome, reproductive failure, and multisystemic inflammation. Here, we investigated and characterized PCV3 from aborted fetuses in Vietnam. We found that the whole genomes of PCV3 collected in these Vietnamese pig farms share 98.4-99.45% sequence identity with reference PCV3 sequences. Several distinct mutation were identified in both the Rep protein and Cap protein of these strains. These strains were clustered into two distinct subtypes (3a1 and 3b). This study contributes to a better understanding of the molecular characteristics and genetic diversity of PCV3 in Vietnam.
Assuntos
Infecções por Circoviridae , Circovirus , Doenças dos Suínos , Feto Abortado , Animais , Infecções por Circoviridae/veterinária , Circovirus/genética , Feminino , Variação Genética , Filogenia , Suínos , VietnãRESUMO
Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) is characterized by high fever, respiratory distress, and high mortality in pigs of all ages and has severely affected the Vietnam pork industry in recent years. The study was conducted to compare the efficacy, safety, and overall performance of a modified live PRRSV-2 vaccine (Fostera PRRS) to an existing PRRSV modified live vaccine on a farm with a recent history of HP-PRRSV-associated respiratory diseases. A total of 351 pigs were randomly allocated to three treatment groups: (i) vaccinated with Fostera PRRS at 1 day of age (n = 118), (ii) vaccinated with Fostera PRRS (n = 118) at 21 days of age, and (iii) vaccinated with Amervac PRRS (n = 115) at 21 days of age. The Fostera PRRS vaccinated pigs had milder clinical symptoms, lower levels of HP-PRRSV viremia, fewer pathological changes in the lung, and higher body weight gain at the end of the study compared with the Amervac PRRS group. Vaccination of pigs with Fostera PRRS at 1 day of age also significantly reduced viral loads in their blood (P < 0.05) and induced higher anti-PRRSV antibody titers (P < 0.01) compared with pigs vaccinated with Amervac PRRS at 21 days of age. Fostera PRRS vaccination at 1 day of age can be useful in protecting young piglets from early HP-PRRSV infection because the immunized pigs were marketed 20 days earlier than their peers immunized at 21-day old as they reached the target market weight earlier in this study.
Assuntos
Anticorpos Antivirais/sangue , Síndrome Respiratória e Reprodutiva Suína/prevenção & controle , Vacinas Virais/imunologia , Animais , Síndrome Respiratória e Reprodutiva Suína/epidemiologia , Síndrome Respiratória e Reprodutiva Suína/virologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/imunologia , Suínos , Vacinação/veterinária , Vacinas Atenuadas/imunologia , Vietnã , Carga Viral , Viremia/imunologiaRESUMO
A total of 34 highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) strains isolated from Vietnam during 2013-2014 were sequenced and analyzed. A partial sequence of ORF1a corresponding to the nonstructural protein 2 (Nsp2) coding region and the full sequence of open reading frame 5 (ORF5) gene was used for the analysis. The HP-PRRSV strains were isolated from pig herds that had never been vaccinated for PRRSV. Nucleotide sequence identities in the portions of ORF1a corresponding to the nonstructural protein 2 (Nsp2) coding region and ORF5 ranged from 96.4 to 100 % and 83.2 to 100 %, respectively. All of the 34 Vietnamese HP-PRRSV strains showed two discontinuous 30-amino-acid deletions in the Nsp2 coding region as a genetic marker of prototypic Chinese HP-PRRSV. The amino acid arginine (R) was present at positions 13 and 151 in ORF5 in 29 out of 34 Vietnamese HP-PRRSV isolates, as well as in the prototypic Chinese HP-PRRSV. Sequence analysis of the ORF5 genes of all Vietnamese HP-PRRSVs revealed six subgroups: Viet 1 to 4, JAX1-like, and VR-2332-like. Nucleotide and amino acid sequence analysis of 34 Vietnamese HP-PRRSV isolates from 2013-2014 indicated that Vietnamese HP-PRRSV has undergone rapid evolutionary changes in recent years when compared with Vietnamese HP-PRRSV isolated before 2012.
Assuntos
Síndrome Respiratória e Reprodutiva Suína/virologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/genética , Vírus da Síndrome Respiratória e Reprodutiva Suína/isolamento & purificação , RNA Viral/genética , Análise de Sequência de DNA , Proteínas do Envelope Viral/genética , Proteínas não Estruturais Virais/genética , Animais , Arginina/genética , Análise por Conglomerados , Evolução Molecular , Marcadores Genéticos , Dados de Sequência Molecular , Filogenia , Vírus da Síndrome Respiratória e Reprodutiva Suína/classificação , Deleção de Sequência , Homologia de Sequência , Suínos , VietnãRESUMO
This study aimed to investigate the co-infection and genetic characteristics of Porcine circoviruses in PMWS-affected pigs in five commercial farrow-to-finish swine farms in Vietnam. By the end of 2022, the percentage of PMWS-affected pigs in these farms has increased significantly compared to previous years. The lymph node samples from ten PMWS typical cases were randomly collected to test for the presence of PRRSV, PCV2, PCV3 and PCV4. While PRRSV and PCV4 were not found in these cases, 10 and 3 out of 10 samples were positive for PCV2 and PCV3, respectively. Three farms in the study showed the co-infection of PCV2 and PCV3 in affected pigs. Besides, all PCV-positive samples were sequenced to evaluate genetic characterization of PCVs in PMWS-affected cases. Phylogenetic analysis showed that all PCV3 strains in the study were clustered into PCV3b genotype. 8 out of 10 PCV2 strains belonged to PCV2d genotype while the remaining two strains belonged to PCV2b genotypes. Two farms had co-circulation of PCV2b and PCV2d genotypes in two different age groups of pigs, which is reported for the first time in Vietnam. Several amino acid substitutions were identified in important antigenic regions in the capsid protein of the PCV2 field strains compared to vaccine strains. Taken together, the results showed the high co-prevalence of PCV3 and PCV2, and the wide genetic diversity of PCV2 field and vaccine strains may be the cause of the increased PMWS situation in these pig farms. Supplementary Information: The online version contains supplementary material available at 10.1007/s13337-023-00849-4.
RESUMO
PURPOSE: This study aimed to investigate the diversity of Balantioides coli (B. coli) based on the 18S rDNA of isolates collected from weaned pigs with diarrhea and then select a B. coli isolate to determine its pathogenicity in weaned pigs through experimental infection. METHODS: The genetic analysis of field-isolated B. coli strains was based on sequencing of the partial 18S rDNA genetic fragment and the pathogenicity of an isolate of B. coli (DN2018-2-DTD) was identified through an experimental infection. RESULTS: The fourteen B. coli isolates shared 98.47-100% nucleotide similarity and were divided into two clades in a phylogenetic tree. In an infection experiment, a slight increase in the body temperature was identified in two infected groups as compared to that in the control group (P < 0.05) from days post-infection (dpi) 12-18 and from dpi 20-27. In the group infected with 3000 CPG/TPG, 2/9 pigs started to develop diarrhea on day two. In the group infected with 500 CPG/TPG, 8/9 pigs had diarrhea on dpi 3. Trophozoites/cysts of B. coli were firstly detected in feces on dpi 3 in a group of pigs infected with a dose of 3000 CPG/TPG, and CPG/TPG reached its peak at dpi 5 in both groups of infected pigs (35,450 and 13,250 CPG/TPG). There was a significant difference in the bodyweight of the control group (noninfected) as compared to that of the groups of infected pigs on dpi 7 (P < 0.05), 14 (P < 0.001) and 28 (P < 0.01). Lesions were solely recorded in infected pigs, including white ulcerative nodules with necrosis scattered in the mucosa of the colon and cecum on dpi 7, 14, and 28. CONCLUSION: This is the first study to investigate the pathogenicity of B. coli in Vietnamese pigs to demonstrate that B. coli could induce necrotic ulcerative enteritis and diarrhea in weaned pigs.
Assuntos
Doenças dos Suínos , Animais , Povo Asiático , Diarreia/veterinária , Fezes , Humanos , Filogenia , Suínos , Virulência , DesmameRESUMO
Classical swine fever (CSF) is a highly contagious, devastating, and transboundary viral disease that afflicts swine industries worldwide. Immunization with vaccines is one of the most effective strategies for controlling this disease. However, shifts in the antigenicity and pathogenicity of novel evolving viral strains have the potential to evade vaccination. In this study, 352 samples from swines exhibiting fever, hemorrhages, lethargy, and diarrhea in different pig farms located in 9 provinces of Vietnam were collected. CSFV was identified even within farms that had been vaccinated against CSFV. Several farms had swine which had been co-infection with CSFV and other pathogens. Copies of the E2 gene of 21 samples were isolated, cloned, sequenced, analyzed, and compared with copies of E2 in four vaccine strains. We identified a total of 42 amino acid substitutions in this glycoprotein, including 11 positions that affect the antigenic properties of E2 and 7 positions that are associated with neutralizing epitopes. The E2 glycoprotein of CSFV strains circulating in Vietnam and vaccine strains differ in their antigenicity. These findings provide deep insights into the molecular characteristics, genetic diversity, pathogenicity, antigenicity, and evolution of CSFV strains in Vietnam. Understanding the pathogenicity, antigenicity, and evolution of circulating CSFV strains will provide avenues for developing new vaccines and efficient approaches to control this disease.
Assuntos
Vírus da Febre Suína Clássica , Peste Suína Clássica , Vacinas Virais , Animais , Anticorpos Antivirais , Peste Suína Clássica/prevenção & controle , Vírus da Febre Suína Clássica/genética , Suínos , Vacinação/veterinária , Vietnã/epidemiologia , Proteínas do Envelope Viral/genética , Vacinas Virais/genéticaRESUMO
African swine fever virus (ASFV), the causative agent of contagious hemorrhagic disease in domestic pigs and wild boars, has temporally regulated gene expression kinetics. The p30 and p72 major structural proteins are involved in viral entry each with different expression kinetics, but neither of their chronological expressions and distribution have been identified in virus-infected animals. Here, we found that both transcription and translation levels of p30 were significantly higher than those of p72 in target organs during the earlier infection-phase. Lymphocyte apoptosis/necrosis and angiectasia were observed as signs of early infection with acute African swine fever. These results show that the chronologically differential expression of ASFV structural proteins tends to be prominent in infected animals, and the p30 protein could play a role in the indication of acute lesions during early infection compared to the late-expressed p72 protein. In conclusion, we propose to consider the chronological expression dynamics of ASFV structural proteins in infected animals to understand virus pathogenesis and antigen targeting for vaccine development.
Assuntos
Vírus da Febre Suína Africana , Febre Suína Africana , Vírus da Febre Suína Africana/genética , Animais , Anticorpos Antivirais , Sus scrofa , Suínos , Internalização do VírusRESUMO
The goal of this study was to identify a candidate commercial cell line for the replication of African swine fever virus (ASFV) by comparing several available cell lines with various medium factors. In the sensitivity test of cells, MA104 and MARC-145 had strong potential for ASFV replication. Next, MA104 cells were used to compare the adaptation of ASFV obtained from tissue homogenates and blood samples in various infectious media. At the 10th passage, the ASFV obtained from the blood sample had a significantly higher viral load than that obtained from the tissue sample (P = 0.000), exhibiting a mean cycle threshold (Ct) value = 20.39 ± 1.99 compared with 25.36 ± 2.11. For blood samples, ASFV grew on infectious medium B more robustly than on infectious medium A (P = 0.006), corresponding to a Ct value = 19.58 ± 2.10 versus 21.20 ± 1.47. African swine fever virus originating from blood specimens continued to multiply gradually and peaked in the 15th passage, exhibiting a Ct value = 14.36 ± 0.22 in infectious medium B and a Ct value = 15.42 ± 0.14 in infectious medium A. When ASFV was cultured from tissue homogenates, however, there was no difference (P = 0.062) in ASFV growth between infectious media A and B. A model was developed to enhance ASFV replication through adaptation to MA104 cells. The lack of mutation at the genetic segments encoding p72, p54, p30, and the central hypervariable region (CVR) in serial culture passages is important in increasing the probability of maintaining immunogenicity when developing a vaccine candidate.
L'objectif de cette étude était d'identifier une lignée cellulaire commerciale candidate pour la réplication du virus de la peste porcine africaine (ASFV) en comparant plusieurs lignées cellulaires disponibles et différents milieux. Lors du test de sensibilité des cellules, MA104 et MARC-145 présentaient un fort potentiel pour la réplication d'AFSV. Par la suite, les cellules MA104 ont été utilisées pour comparer l'adaptation d'ASFV obtenu d'homogénats de tissus et d'échantillons de sang dans différents milieux. Au dixième passage, l'ASFV obtenu de l'échantillon de sang avait une charge virale significativement plus élevée que celle obtenue de l'échantillon de tissu (P = 0,000), avec une valeur seuil moyenne de cycles (Ct) de 20,39 ± 1,99 comparativement à 25,36 ± 2,11. Pour les échantillons sanguins, l'ASFV a poussé sur le milieu B de manière plus robuste que sur le milieu A (P = 0,006), ce qui correspond à une valeur Ct de 19,58 ± 2,10 versus 21,20 ± 1,47. L'ASFV provenant des échantillons sanguins continua de se multiplier graduellement et atteignit un pic au 15e passage, avec une valeur Ct de 14,36 ± 0,22 dans le milieu B et une valeur Ct de 15,42 ± 0,14 dans le milieu A. Toutefois, lorsque l'ASFV fut cultivé à partir des homogénats de tissus, il n'y avait pas de différence (P = 0,062) dans la croissance d'ASFV entre les milieux A et B. Un modèle a été développé pour augmenter la réplication d'ASFV par adaptation aux cellules MA104. L'absence de mutation au segment génétique codant pour p72, p54, p30, et la région hypervariable centrale (CVR) dans des passages en série en culture est importante en augmentant la probabilité de maintenir une immunogénicité lors du développement d'un vaccin candidat.(Traduit par Docteur Serge Messier).
Assuntos
Vírus da Febre Suína Africana , Febre Suína Africana , Doenças dos Suínos , Vírus da Febre Suína Africana/genética , Animais , Genótipo , Mutação , Inoculações Seriadas/veterinária , SuínosRESUMO
Classical swine fever virus (CSFV) is an RNA virus that incurs severe economic costs to swine industries worldwide. This study was conducted to investigate the genetic diversity among CSFV strains circulating in Vietnam, with a focus on their genetic variants relative to four vaccine strains. Samples from clinical cases were collected from different provinces of Central and Southern Vietnam from 2017 to 2019. 21 CSFV-positive samples were selected for amplification and sequencing of the full-length Erns and E2 genes. Phylogenetic analyses of these two genes showed that most CSFV strains circulating in Central and Southern Vietnam from 2017 to 2019 belong to subgroup 2.1c, whereas the remaining strains cluster into subgroup 2.2. All CSFV field strains in this study were genetically distant from group 1 strains. Analysis of the E2 and Erns genes indicated that all CSFV field strains have low sequence identity with the vaccine strains (80-83.5% and 82.3-86% sequence identity for E2 and Erns, respectively). Likewise, amino acid-level sequence analysis showed 87.3-91.1% and 87.6-91.6% sequence identity for E2 and Erns, respectively. Together, our findings indicate that CSFV strains circulating in Vietnam belong to subtypes 2.2 and 2.1c, and we also provide novel insights into the epidemiology, molecular characteristics, genetic diversity, and evolution of these circulating CSFV strains.
Assuntos
Vírus da Febre Suína Clássica/genética , Variação Genética , Glicoproteínas de Membrana/genética , Proteínas do Envelope Viral/genética , Animais , Peste Suína Clássica/virologia , Filogenia , Sus scrofa , Suínos , VietnãRESUMO
African swine fever (ASF) causes a contagious hemorrhagic disease in all ages of pigs without sex predilections. The objective of this study was to determine the age-related viral loads and severity of systemic pathological lesions among three different swine group ages (weaned pigs, fattening pigs, and sows) during a recent outbreak of acute ASF in Vietnam. Age-related viral loads were determined in 5 major organs (lung, liver, spleen, kidney, and lymph node) by immunohistochemistry as well as in the blood by real-time polymerase chain reaction (PCR). Age-related systemic pathological lesions were analyzed in the listed organs among three age groups. Weaned pigs had significantly (p < 0.05) higher levels of viral loads in their lung, liver, lymph nodes and blood than in those of fattening pigs and sows. Fattening pigs had significantly (p < 0.05) higher scores of macroscopic lung and lymphoid lesions, and microscopic liver lesions compared with those of weaned pigs and sows. The results of this study demonstrated that viral loads were age-related in acute naturally occurring ASF but the severity of pathological lesions was not correlated with the level of viral loads in the five major organs.
Assuntos
Vírus da Febre Suína Africana , Febre Suína Africana , Doenças dos Suínos , Febre Suína Africana/epidemiologia , Vírus da Febre Suína Africana/genética , Animais , Surtos de Doenças , Feminino , Genótipo , Sus scrofa , Suínos , Carga Viral/veterináriaRESUMO
African swine fever (ASF) is a contagious haemorrhagic disease in pigs and has become endemic in several Vietnam provinces since the first outbreak in 2019. The presence of carriers and the recurrence of disease in the surviving swine herd after an ASF outbreak has not previously been properly evaluated. In this study, pigs naturally infected with an acute form of ASF were allowed to recover from the disease. A serological follow-up was conducted for more than 14 months with 14 convalescent gilts and their offspring. All convalescent animals had long lasting high serum antibody levels without persistent viremia. They also did not excrete virus via nasal discharge post-recovery. These convalescent pigs could partially perform as replacement gilts despite the fact that ASF affected reproductive performance. Here, we confirmed that there were neither the carriers of nor recurrence of disease in the convalescent pigs and their offspring following the outbreak of acute ASF. These findings may facilitate efforts to design a new farming model in ASF endemic provinces in Vietnam where there is a lack of a repopulation strategy due to the limited funding received from the local regulatory authorities.
Assuntos
Vírus da Febre Suína Africana , Febre Suína Africana , Doenças dos Suínos , Febre Suína Africana/epidemiologia , Vírus da Febre Suína Africana/genética , Animais , Surtos de Doenças/veterinária , Feminino , Seguimentos , Sus scrofa , Suínos , Vietnã/epidemiologiaRESUMO
The aim of this study was to evaluate the protective efficacy of the CIRCOQ porcine circovirus type 2 (PCV2) subunit vaccine in piglets with high maternally derived antibodies (MDAs) against disease caused by natural infection with PCV2d. A total of 130 weaned, 21-day-old healthy pigs was allocated into 3 trial groups. The signs of respiratory disorder were higher in unvaccinated pigs than in vaccinated pigs at 13 to 17 weeks old (P < 0.05), 18 to 22 weeks old (P < 0.001), and 23 to 27 weeks old (P < 0.01). The unvaccinated pigs had an early rate of dermatitis at 8 to 12 weeks old (10.0%), 13 to 17 weeks old (30.0%), 18 to 22 weeks old (46.7%), and 23 to 27 weeks old (33.3%), while there were no cases of dermatitis in vaccinated pigs. There was a significant difference (P < 0.05) in the mortality of pigs in the unvaccinated group and the 2-dosed vaccinated group. PCV2 viremia was detected in the blood and peaked at 105 days old in both unvaccinated pigs (Ct-adj = 8.40) and pigs vaccinated with 1 dose (Ct-adj = 6.37), while no detectable PCV2 virus was found in the blood of pigs vaccinated with 2 doses. At 77 and 105 days old, the PCV2 viremia load (Ct-adj) of unvaccinated pigs and those vaccinated with 1 dose was significantly higher (P < 0.05) than that of the 2-dosed vaccinated pigs. The body weight (BW), average weight gain (AWG), and average daily gain (ADG) in both groups of vaccinated pigs were significantly higher (P < 0.05) than those of unvaccinated pigs. The study vaccine was significantly efficacious in protecting vaccinated pigs against clinical symptoms, blood viral load, and mortality, as well as improving productivity, compared with unvaccinated pigs.
Le but de la présente étude était d'évaluer l'efficacité protectrice du vaccin sous-unitaire CIRCOQ du circovirus porcin de type 2 (PCV2) chez les porcelets ayant une grande quantité d'anticorps d'origine maternelle (MDA) contre la maladie causée par une infection naturelle par le PCV2d. Un total de 130 porcs sains sevrés âgés de 21 jours a été réparti dans trois groupes d'essai. Les signes de troubles respiratoires étaient plus élevés chez les porcs non vaccinés que chez les porcs vaccinés âgés de 13 à 17 semaines (P < 0,05), de 18 à 22 semaines (P < 0,001) et de 23 à 27 semaines (P < 0,01). Les porcs non vaccinés avaient un taux précoce de dermatite entre 8 et 12 semaines (10,0 %), 13 à 17 semaines (30,0 %), 18 à 22 semaines (46,7 %) et 23 à 27 semaines (33,3 %), alors qu'il n'y a eu aucun cas de dermatite chez les porcs vaccinés. Il y avait une différence significative (P < 0,05) dans la mortalité des porcs dans le groupe non vacciné et le groupe vacciné à deux doses. La virémie du PCV2 a été détectée dans le sang et a atteint un pic à 105 jours chez les porcs non vaccinés (Ct-adj = 8,40) et les porcs vaccinés avec une dose (Ct-adj = 6,37), tandis qu'aucun virus PCV2 détectable n'a été détecté dans le sang des porcs vacciné avec deux doses. À 77 et 105 jours, la charge de virémie PCV2 (Ct-adj) des porcs non vaccinés et de ceux vaccinés avec une dose était significativement plus élevée (P < 0,05) que celle des porcs vaccinés à deux doses. Le poids corporel (BW), le gain de poids moyen (AWG) et le gain quotidien moyen (ADG) dans les deux groupes de porcs vaccinés étaient significativement plus élevés (P < 0,05) que ceux des porcs non vaccinés. Le vaccin de l'étude s'est avéré significativement efficace pour protéger les porcs vaccinés contre les symptômes cliniques, la charge virale sanguine et la mortalité, ainsi que pour améliorer la productivité, par rapport aux porcs non vaccinés.(Traduit par Docteur Serge Messier).
Assuntos
Infecções por Circoviridae/veterinária , Circovirus/imunologia , Esquemas de Imunização , Doenças dos Suínos/prevenção & controle , Vacinas Virais/imunologia , Animais , Infecções por Circoviridae/sangue , Infecções por Circoviridae/prevenção & controle , Infecções por Circoviridae/virologia , Circovirus/genética , Relação Dose-Resposta Imunológica , Imunidade Materno-Adquirida , Imunoglobulina G/sangue , Transmissão Vertical de Doenças Infecciosas/veterinária , Interferon gama/sangue , Filogenia , Suínos , Doenças dos Suínos/sangue , Doenças dos Suínos/imunologia , Doenças dos Suínos/patologia , Vietnã/epidemiologia , Carga Viral , Vacinas Virais/administração & dosagem , ViremiaRESUMO
African swine fever virus (ASFV) causes hemorrhagic disease in domestic pigs by replicating mainly in monocyte/macrophage lineages. Various primary cells including pulmonary alveolar macrophages have been used for the propagation of ASFV on this account. However, ethical constraints and consistency problems exist as it is necessary to harvest same phenotype of primary cells in order to continue a study. We suggested renal-derived swine macrophages as a novel primary cell candidate to address these issues. These primary cells proved to be permissive to both cell adapted ASFV and a wild-type ASFV. Compared to the commercial cell line MA-104, the renal-derived macrophages were more suitable to isolate the field virus. The consistent molecular characteristics of the renal-derived macrophages were demonstrated by immunocytochemistry with antibodies against macrophage cell surface markers including CD163, CD172a, and Iba-1. Viral protein p30 and p72 expression in ASFV infected macrophages was confirmed by immunocytochemistry by use of specific monoclonal antibodies. We observed increase of cell-free viral DNA and infectious virus titer in infected cell supernatant in successive days-post-infection. These results demonstrated that primary renal-derived swine macrophages are useful for ASFV isolation and propagation in terms of cell phenotypes, susceptibility to the virus, and virus production.
RESUMO
Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) known as pig high fever disease was first reported in China and has spread rapidly in neighboring southeastern Asian countries. The objective of this study was to evaluate the efficacy of a new type 2 PRRSV modified live vaccine (vaccine A) against a challenge with a HP-PRRSV and to compare the efficacy of two genetically distant type 2 PRRSV modified vaccines (vaccine A for lineage 8 and vaccine B for lineage 5) against HP-PRRSV (lineage 8) challenge. Pigs were divided into 4 groups (n=12/group); vaccinated challenged (2 groups), unvaccinated challenged, and unvaccinated unchallenged groups. Regardless of vaccines, vaccinated challenged pigs showed significantly lower (P<0.05) mean rectal temperatures and respiratory scores, levels of HP-PRRSV viremia, and lung lesions and HP-PRRSV antigens within lung lesions compared to unvaccinated challenged pigs. Vaccinated challenged pigs had significantly higher (P<0.05) numbers of interferon-γ secreting cells (IFN-γ-SC) compared to unvaccinated challenged pigs. Significant differences were also found when comparing two type 2 PRRSV vaccines after HP-PRRSV challenge. The use of type 2 PRRSV vaccine A was able to significantly reduce fever when compared to type 2 PRRSV vaccine B in vaccinated challenged pigs. Vaccination of pigs with vaccine A reduced viral loads in their blood and induced higher numbers of HP-PRRSV-specific IFN-γ-SC than vaccination of pigs with vaccine B. This study demonstrates partial protection of two genetically distant type 2 PRRSV vaccines against HP-PRRSV challenge in growing pigs.