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1.
Zhonghua Yi Xue Za Zhi ; 103(39): 3083-3087, 2023 Oct 24.
Artigo em Zh | MEDLINE | ID: mdl-37840179

RESUMO

Pain is the fifth major vital sign, and chronic pain is a large category of diseases that affects health seriously. At present, the incidence of chronic pain is high, but the overall treatment satisfaction is low. It is necessary to continuously optimize pain diagnosis and treatment strategies and improve the connotation of pain management. Based on the clinical practice of our pain center, combined with relevant literature, the article proposes a diagnosis and treatment strategy of "whole field pain management" should be carried out from the four dimensions of feeling, emotion, cognition, and behavior. Innovative digital pain diagnosis and treatment technologies such as VR/MR and brain-computer interface are used to regulate emotional, cognitive, and behavioral regulation, and combined with lifestyle changes, rehabilitation physiotherapy, drugs, and minimally invasive interventional therapy to constitute a " whole field pain management strategy" to explore the new development direction of further improving the management of chronic pain.


Assuntos
Dor Crônica , Manejo da Dor , Humanos , Manejo da Dor/métodos , Dor Crônica/diagnóstico , Dor Crônica/terapia , Modalidades de Fisioterapia , Emoções , Cognição
2.
Cytopathology ; 22(6): 365-72, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21054589

RESUMO

OBJECTIVE: To compare the performance of different assays for measuring apoptosis in cytological specimens. METHODS: Apoptosis was assessed in 27 specimens (22 effusions, five fine needle aspirates; 20 malignant, seven reactive) using flow cytometry, applying assays for the measurement of annexin V expression, caspase-3 and -8 cleavage and deoxynucleotidyl transferase deoxyuridine triphosphates (dUTP) incorporation. Results were studied for differences between reactive and malignant specimens, as well as performance across assays. RESULTS: Wide variation in the degree of apoptosis was observed in both benign and malignant specimens using all assays. However, the percentage of annexin V-positive cells was higher compared with those showing caspase cleavage or dUTP incorporation in the majority of cases, irrespective of specimen type. Comparative analysis of benign and malignant specimens showed no significant differences in expression of any of the studied parameters. However, tumour cells and reactive mesothelial cells in pleural effusions had a significantly lower level of dUTP incorporation compared with their counterparts in peritoneal specimens (P = 0.001). CONCLUSIONS: The present data are in agreement with our previous observation in ovarian carcinoma effusions, that measurement of apoptosis by the annexin V assay provides higher expression values than those obtained by other assays, suggesting that this assay does not accurately reflect the degree of apoptosis in benign or malignant cells in effusions.


Assuntos
Anexina A5/metabolismo , Apoptose , Biópsia por Agulha Fina/métodos , Caspases/genética , Citometria de Fluxo/métodos , Marcação In Situ das Extremidades Cortadas/métodos , Líquido Ascítico/metabolismo , Humanos , Metástase Neoplásica/patologia , Derrame Pleural/metabolismo
3.
Cytopathology ; 22(1): 22-9, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-20500520

RESUMO

OBJECTIVE: The PINCH-2 gene was previously shown to be overexpressed in malignant mesothelioma compared with ovarian/peritoneal serous carcinoma in Affymetrix array analysis. The objective of the present study was to validate this finding at the mRNA and protein level. METHODS: Effusions (n = 91; 71 ovarian and 10 breast carcinomas, 10 malignant mesotheliomas) were assayed for PINCH-2 mRNA expression using quantitative PCR. PINCH-2 protein expression was analysed in 37 effusions using flow cytometry. RESULTS: Quantitative PCR analysis showed significantly higher PINCH-2 mRNA levels in mesotheliomas compared with carcinomas (P = 0.004). Values of <10 copies were found exclusively in carcinoma effusions (25.4% of ovarian and 50% of breast carcinomas). However, PINCH-2 protein expression by flow cytometry did not differ significantly between the three cancer types. No association was observed between PINCH-2 levels and patient survival or expression of previously-studied molecules related to adhesion, metastasis and apoptosis inhibition in ovarian carcinoma. CONCLUSIONS: Our data suggest that PINCH-2 mRNA is overexpressed in malignant mesothelioma compared with carcinomas involving serosal cavities, and that low levels of this gene argue against the diagnosis of mesothelioma. The frequent PINCH-2 protein expression in all three studied cancers suggests a role for this molecule in cancer cell biology in effusions and merits further research.


Assuntos
Adenocarcinoma/genética , Neoplasias da Mama/genética , Proteínas de Ligação a DNA/genética , Regulação Neoplásica da Expressão Gênica , Mesotelioma/genética , Neoplasias Císticas, Mucinosas e Serosas/genética , Neoplasias Ovarianas/genética , Proteínas Adaptadoras de Transdução de Sinal , Adenocarcinoma/diagnóstico , Adenocarcinoma/patologia , Biomarcadores Tumorais/análise , Neoplasias da Mama/diagnóstico , Neoplasias da Mama/patologia , Primers do DNA , Proteínas de Ligação a DNA/metabolismo , Diagnóstico Diferencial , Exsudatos e Transudatos , Feminino , Citometria de Fluxo , Perfilação da Expressão Gênica , Humanos , Proteínas com Domínio LIM , Masculino , Proteínas de Membrana , Mesotelioma/diagnóstico , Mesotelioma/patologia , Neoplasias Císticas, Mucinosas e Serosas/diagnóstico , Neoplasias Císticas, Mucinosas e Serosas/patologia , Neoplasias Ovarianas/diagnóstico , Neoplasias Ovarianas/patologia , Reação em Cadeia da Polimerase , RNA Mensageiro/metabolismo
4.
Cytopathology ; 22(6): 373-82, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22032542

RESUMO

OBJECTIVE: The objective of this study was to establish a flow cytometry assay for measuring c-FLIP in serous effusions. In addition, we studied the clinical relevance in ovarian carcinoma effusions of this inhibitor protein in the death receptor signalling pathway of apoptosis. METHODS: Two c-FLIP antibodies were tested using Western blotting and the best performing one was used for titration of c-FLIP expression in a panel of five cell lines, consisting of ovarian carcinoma, breast carcinoma and malignant mesothelioma. The concentration that provided the best signal-to-noise ratio was used for comparison of the performance of three fixation and permeabilization protocols. The best performing protocol was chosen for analysis of 69 ovarian carcinoma effusions. c-FLIP expression was analysed for association with clinicopathological parameters and survival. RESULTS: Rabbit polyclonal c-FLIP by Abcam and the IntraStain kit by Dako performed best. c-FLIP expression was detected in tumour cells in all 69 effusions (expression range 21-100%, median = 80%). No association was found between c-FLIP expression and clinicopathological parameters, including chemoresponse and survival. However, an inverse correlation was found between c-FLIP levels and expression of the previously studied apoptosis marker cleaved caspase-3 (P = 0.029). CONCLUSIONS: An assay for measuring c-FLIP in cytology specimens is presented. c-FLIP is frequently expressed in ovarian carcinoma effusions, but its expression appears to be unrelated to disease aggressiveness.


Assuntos
Adenocarcinoma/metabolismo , Proteína Reguladora de Apoptosis Semelhante a CASP8 e FADD/metabolismo , Exsudatos e Transudatos/metabolismo , Citometria de Fluxo/métodos , Neoplasias Ovarianas/metabolismo , Derrame Pleural/patologia , Adenocarcinoma/mortalidade , Adenocarcinoma/patologia , Idoso , Idoso de 80 Anos ou mais , Apoptose , Caspase 3/metabolismo , Feminino , Humanos , Métodos , Pessoa de Meia-Idade , Neoplasias Ovarianas/mortalidade , Neoplasias Ovarianas/patologia , Derrame Pleural/metabolismo , Análise de Sobrevida , Células Tumorais Cultivadas
5.
Braz J Med Biol Res ; 51(5): e6714, 2018 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-29561955

RESUMO

This study aimed to investigate the protective effect of salvinorin A on the cerebral pial artery after forebrain ischemia and explore related mechanisms. Thirty Sprague-Dawley rats received forebrain ischemia for 10 min. The dilation responses of the cerebral pial artery to hypercapnia and hypotension were assessed in rats before and 1 h after ischemia. The ischemia reperfusion (IR) control group received DMSO (1 µL/kg) immediately after ischemia. Two different doses of salvinorin A (10 and 20 µg/kg) were administered following the onset of reperfusion. The 5th, 6th, and 7th groups received salvinorin A (20 µg/kg) and LY294002 (10 µM), L-NAME (10 µM), or norbinaltorphimine (norBIN, 1 µM) after ischemia. The levels of cGMP in the cerebrospinal fluid (CSF) were also measured. The phosphorylation of AKT (p-AKT) was measured in the cerebral cortex by western blot at 24 h post-ischemia. Cell necrosis and apoptosis were examined by hematoxylin-eosin staining (HE) and TUNEL staining, respectively. The motor function of the rats was evaluated at 1, 2, and 5 days post-ischemia. The dilation responses of the cerebral pial artery were significantly impaired after ischemia and were preserved by salvinorin A treatment. In addition, salvinorin A significantly increased the levels of cGMP and p-AKT, suppressed cell necrosis and apoptosis of the cerebral cortex and improved the motor function of the rats. These effects were abolished by LY294002, L-NAME, and norBIN. Salvinorin A preserved cerebral pial artery autoregulation in response to hypercapnia and hypotension via the PI3K/AKT/cGMP pathway.


Assuntos
Isquemia Encefálica/metabolismo , Artérias Cerebrais/efeitos dos fármacos , GMP Cíclico/líquido cefalorraquidiano , Diterpenos Clerodânicos/farmacologia , Fosfatidilinositol 3-Quinases/metabolismo , Pia-Máter/irrigação sanguínea , Proteínas Proto-Oncogênicas c-akt/metabolismo , Animais , Isquemia Encefálica/tratamento farmacológico , Artérias Cerebrais/fisiopatologia , Cromonas/administração & dosagem , GMP Cíclico/metabolismo , Modelos Animais de Doenças , Diterpenos Clerodânicos/antagonistas & inibidores , Masculino , Morfolinas/administração & dosagem , NG-Nitroarginina Metil Éster , Naltrexona/administração & dosagem , Naltrexona/análogos & derivados , Ratos , Ratos Sprague-Dawley , Transdução de Sinais
6.
Eur Rev Med Pharmacol Sci ; 21(16): 3658-3664, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28925477

RESUMO

OBJECTIVE: In this study, we adopted maternal diabetes model on rats, which induced by streptozotocin to explore the metabolism changes of rat adipose tissue during the neonatal period. MATERIALS AND METHODS: The female rats were induced as diabetes models by streptozotocin (STZ), and mated with the normal male rats when they entered into adulthood. The chosen male offspring rats were executed at week 12 and the epididymis and subcutaneous fat pad were obtained. Then the adipose cells were extracted and the foundation level and absorbing of insulin induced 2-deoxyglucose (2DG) were assessed. RESULTS: Compared with normal control group, the body weight, fat pad weight of the epididymis and diameter of lipid cells for maternal diabetes offspring rats all increased. Lipid cells of epididymis and the intake of glucose induced by insulin increased. At the same time, glucose was oxidized to CO2 and increased lipid. However, there was no change in the capacity of in vitro lipid decomposition. Also, GLUT4, insulin receptor (IRß), acetyl coenzyme A (ACC), etc. increased in fat pad of maternal offspring of diabetes. CONCLUSIONS: Maternal diabetes had effect on fat metabolism of offspring; lipid storage capacity increased but the ability of lipid decomposition had no change.


Assuntos
Tecido Adiposo/metabolismo , Diabetes Mellitus Experimental/metabolismo , Diabetes Gestacional/metabolismo , Hiperglicemia/metabolismo , Animais , Feminino , Glucose/metabolismo , Metabolismo dos Lipídeos , Masculino , Gravidez , Ratos , Estreptozocina
7.
Thromb Haemost ; 75(1): 101-6, 1996 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-8713787

RESUMO

Thrombolytic therapy is known to induce platelet-related side effects. We used a parallel-plate flow chamber, which was connected to the femoral artery of the rat, to measure platelet adhesion ex vivo. A collagen-coated arterioarterial shunt between two carotid arteries was used to measure shunt patency duration as an index of antithrombotic efficacy. Tissue-type plasminogen activator (t-PA), vitamin E, and the combination of these two were intravenously administered for 60 min. Measurements were performed before drug administration, and at 30, 60, 120 min after the initiation of drug infusion. Our results indicated that (1) treatments with t-PA or t-PA/vitamin E prolonged the time to shunt occlusion at 30 and 60 min; (2) t-PA enhanced platelet adhesion at 60 and 120 min; (3) vitamin E tended to reduce platelet adhesion; (4) t-PA/vitamin E reduced the t-PA-enhanced platelet adhesion; (5) at the high-density area of platelet adhesion under t-PA treatment, the adherent platelets demonstrated severe morphological changes which could be blocked by vitamin E. These data suggest that t-PA may enhance platelet adhesion in rats and that this adverse effect can be suppressed by co-administration of vitamin E.


Assuntos
Fístula Artério-Arterial , Trombose das Artérias Carótidas/tratamento farmacológico , Adesividade Plaquetária/efeitos dos fármacos , Ativador de Plasminogênio Tecidual/farmacologia , Vitamina E/farmacologia , Análise de Variância , Animais , Quimioterapia Combinada , Masculino , Ratos , Ratos Sprague-Dawley , Fatores de Tempo , Ativador de Plasminogênio Tecidual/antagonistas & inibidores
8.
J Clin Pathol ; 53(7): 513-7, 2000 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-10961174

RESUMO

AIM: To evaluate immunophenotyping by means of flow cytometry as a complementary method for the detection of malignant cells in serous effusions and peritoneal washings. MATERIAL AND METHODS: Frozen samples of 49 fresh serous effusions and peritoneal washings were analysed by flow cytometry, using monoclonal antibodies against CD45, Ber-EP4, and N-cadherin. Results were compared with smear and cell block morphology, as well as immunocytochemistry on paraffin wax embedded cell blocks. RESULTS: Seventeen specimens were cytologically diagnosed as malignant, whereas 25 were interpreted as benign. The remaining seven specimens were diagnosed as indeterminate or suspicious for malignancy. Ber-EP4 positive cells were detected in 16 of the 17 cytologically malignant effusions, as well as in five of seven suspicious cases and five of 25 specimens with benign cytology. In the latter group, three specimens showed atypical or malignant cell groups that were missed in routine morphological evaluation. In two additional samples, obtained from patients with benign and borderline ovarian tumours, Ber-EP4 positive cells showed benign or mildly atypical features, and were interpreted as exfoliated benign or borderline malignant epithelial cells of tubal origin, or as endosalpingiosis. All five Ber-EP4 positive indeterminate specimens showed atypical or malignant cells on re-evaluation, and were Ber-EP4 positive in four of five cases using immunohistochemistry in cell block sections. Large numbers of CD45 positive and relatively few N-cadherin positive cells were detected in most specimens with the use of flow cytometry, when compared with morphological evaluation. CONCLUSIONS: Flow cytometry is a rapid and highly effective method for the evaluation of effusions and peritoneal washings. The detection of Ber-EP4 positive cells using flow cytometry is strongly indicative of the presence of carcinoma cells in effusions and peritoneal washings. Although false positives are relatively infrequent, all specimens should be carefully evaluated morphologically to prevent the diagnosis of benign epithelial clusters as malignant.


Assuntos
Líquido Ascítico/patologia , Biomarcadores Tumorais , Imunofenotipagem/métodos , Neoplasias/diagnóstico , Adulto , Idoso , Anticorpos Monoclonais , Antígenos de Superfície/metabolismo , Líquido Ascítico/imunologia , Caderinas/metabolismo , Feminino , Citometria de Fluxo/métodos , Humanos , Imuno-Histoquímica/métodos , Antígenos Comuns de Leucócito/metabolismo , Masculino , Pessoa de Meia-Idade , Sensibilidade e Especificidade
9.
Diagn Cytopathol ; 25(4): 214-9, 2001 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11599103

RESUMO

The objective of the present study was to compare the efficiency of immunophenotyping using flow cytometry (FCM) and immunocytochemistry (ICC) in the detection of macrophages in serous effusions. Cytoblock sections from 90 effusions were stained for the monocyte/macrophage marker CD14, using ICC. Fresh-frozen samples of all cases were analyzed for CD14 expression, using FCM. Epithelial, lymphoid, and mesothelial cell populations were identified using antibodies against Ber-EP4, CD45, and N-cadherin, respectively. Results were compared with clinical parameters and morphological diagnosis. Thirty-nine specimens were cytologically diagnosed as malignant, containing tumor cells of nonhematologic origin, whereas 46 were interpreted as benign. Two additional specimens were diagnosed as indeterminate or suspicious for malignancy, and 3 specimens contained lymphoma cells. CD14-positive cells were detected in 85/90 (94%) of effusions using FCM, and in all 90 specimens using ICC. The percentage of CD14-positive cells was highly variable, but in some specimens was as high as 76% using FCM and 85% using ICC. A good association was observed between the two methods in the detection of CD14-positive cells (P < 0.001). The presence of macrophages in effusions showed an association with female gender, using both FCM (P = 0.002) and ICC (P = 0.011), but none with effusion site, patient age, clinical and cytological diagnosis, or presence of Ber-EP4-positive cells (P > 0.05). The presence of Ber-EP4-positive cells showed a strong association with the cytological diagnosis of malignancy (P < 0.001). In conclusion, macrophages are a significant cell population in effusions, of both benign and malignant etiology, due to both their size and their possible confusion with cancer cells. Both FCM and ICC aid in the recognition of these cells, and thus provide an effective tool for the identification of different cell populations in effusions.


Assuntos
Exsudatos e Transudatos/citologia , Exsudatos e Transudatos/imunologia , Macrófagos/patologia , Monócitos/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Líquido Ascítico/imunologia , Líquido Ascítico/patologia , Exsudatos e Transudatos/química , Feminino , Citometria de Fluxo/métodos , Humanos , Imuno-Histoquímica/métodos , Imunofenotipagem/métodos , Macrófagos/química , Masculino , Pessoa de Meia-Idade , Monócitos/química , Derrame Pleural/imunologia , Derrame Pleural/patologia , Derrame Pleural Maligno/química , Derrame Pleural Maligno/patologia
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