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1.
Sensors (Basel) ; 22(12)2022 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-35746326

RESUMO

In this paper, double slot podal and antipodal ultra-wideband (UWB) microstrip antennas for a fluid property measurement system are proposed. Among different feeding techniques, out of phase uni-planner power divider approach is used. The performance verification of the proposed antenna is explained, along with a performance comparison of the antenna bandwidth, feeding, and the realized gain. The suggested podal antenna has an impedance bandwidth from 2.4 to 15.4 GHz, with a maximum gain of 11.3 dBi in the 12 GHz region while the antipodal antenna has a 2.8 GHz to 16 GHz impedance bandwidth, with a maximum gain of 10.4 dBi in the 10 GHz region. Within the intended band, the radiation pattern had an excellent directivity characteristic. The implementation of the proposed antenna is calibrated by measuring the propagated signals response via various liquid specimens using UWB radar, which might be applied for fluid sensing and prediction purposes. The proposed antenna was connected to an NVA-R661 module of Xethru Inc. for measuring the sample delay and peak-to-peak amplitude of the received signals passing through specimens. The measured parameters at a different radar frequency range of transmission are applied by drawing the fluid viscous analogy based on Poiseuille's law hypothesis, showing clear differentiation between the test specimens.

2.
Sensors (Basel) ; 22(22)2022 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-36433530

RESUMO

In this study, a multiple-input multiple-output (MIMO) antenna for wide scanning is designed. By adding slits to the patches, each element is intended to strengthen the isolation between the radiating elements. The proposed high isolation and wide scanning antenna combine to achieve the desired phased-array antenna. The array has a main beam pointing to the desired scanning region and a minimum side lobe level (SLL) in the undesired direction. A compact and linear eight-element array with an interelement spacing of λ/2 is designed and analyzed for beam scanning in the E-plane. Considering the worst case, the proposed array has a very low mutual coupling of (S21 = -24 dB), and it realizes a gain of 9.3 dBi and an SLL of 11 dB at a scanning angle of 70∘. The antenna performance was studied in terms of the S-parameter, radiation patterns, beam-scanning performance, envelope correlation coefficient (ECC), diversity gain (DG), peak gain, and efficiency. A close agreement was observed between the simulated and measured results.

3.
Sensors (Basel) ; 22(17)2022 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-36081050

RESUMO

The millimeter-wave (mmWave) frequency is considered a viable radio wave band for fifth-generation (5G) mobile networks, owing to its ability to access a vast spectrum of resources. However, mmWave suffers from undesirable characteristics such as increased attenuation during transmission. Therefore, a well-fitted path loss model to a specific environment can help manage optimal power delivery in the receiver and optimal transmitter power in the transmitter in the mmWave band. This study investigates large-scale path loss models in a university hall environment with a real-measured path loss dataset using directional horn antennas in co-polarization (H-H) and tracking antenna systems (TAS) in line-of-sight (LOS) circumstances between the transmitter and receptor at mmWave and centimeter-level bands. Although the centimeter-level band is used in certain industrialized nations, path loss characteristics in a university hall environment have not been well-examined. Consequently, this study aims to bridge this research gap. The results of this study indicate that, in general, the large-scale floating-intercept (FI) model gives a satisfactory performance in fitting the path loss both in the center and wall side links.

4.
Sensors (Basel) ; 22(23)2022 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-36502255

RESUMO

In this paper, a horn-shaped strip antenna exponentially tapered carved on a multilayer dielectric substrate for an indoor body position tracking system is proposed. The performance of the proposed antenna was verified by testing it as a tracking state of an indoor resting body position. Among different feeding techniques, the uniplanar T-junction power divider approach is used. The performance verification of the proposed antenna is explained through its compact size and 3D shape, along with a performance comparison of the return loss radiation pattern and the realized gain. The suggested antenna has an 88.88% fractional bandwidth and a return loss between 6 and 15.6 GHz, with a maximum gain of 9.46 dBi in the 9.5 GHz region. Within the intended band, the radiation pattern had an excellent directivity characteristics. The proposed antenna was connected to an NVA-R661 module of Xethru Inc. for sleeping body position tracking. The performance of the antenna is measured through microwave imagining of the state of the resting body in various sleeping positions on the bed using a Recurrent Neural Network (RNN). The predicted outcomes clearly define the antenna's performance and could be used for sensing and prediction purposes.


Assuntos
Inteligência Artificial , Postura , Redes Neurais de Computação , Descanso , Sono
5.
Sensors (Basel) ; 22(14)2022 Jul 12.
Artigo em Inglês | MEDLINE | ID: mdl-35890888

RESUMO

This paper presents the performance comparison of a dual-band conventional antenna with a split-ring resonator (SRR)- and electromagnetic bandgap (EBG)-based dual-band design operating at 2.4 GHz and 5.4 GHz. The compactness and dual-frequency operation in the legacy Wi-Fi range of this design make it highly favorable for wearable sensor network-based Internet of Things (IoT) applications. Considering the current need for wearable antennas, wash cotton (with a relative permittivity of 1.51) is used as a substrate material for both conventional and metamaterial-based antennas. The radiation characteristics of the conventional antenna are compared with the EBG and SRR ground planes-based antennas in terms of return loss, gain, and efficiency. It is found that the SRR-based antenna is more efficient in terms of gain and surface wave suppression as well as more compact in comparison with its two counterparts. The compared results are found to be based on two distinct frequency ranges, namely, 2.4 GHz and 5.4 GHz. The suggested SRR-based antenna exhibits improved performance at 5.4 GHz, with gains of 7.39 dbi, bandwidths of 374 MHz, total efficiencies of 64.7%, and HPBWs of 43.2 degrees. The measurements made in bent condition are 6.22 db, 313 MHz, 52.45%, and 22.3 degrees, respectively. The three considered antennas (conventional, EBG-based, and SRR-based) are designed with a compact size to be well-suited for biomedical sensors, and specific absorption rate (SAR) analysis is performed to ensure user safety. In addition, the performance of the proposed antenna under bending conditions is also considered to present a realistic approach for a practical antenna design.


Assuntos
Internet das Coisas , Dispositivos Eletrônicos Vestíveis , Desenho de Equipamento
6.
Entropy (Basel) ; 24(7)2022 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-35885210

RESUMO

Based on the examination of the fundamental characteristics of structures, structural health monitoring (SHM) has received increased attention in recent years. Studies have shown that the SHM method using entropy analysis can precisely identify the damaged location of the structure, which is very helpful for the daily inspection or maintenance of civil structures. Although entropy analysis has shown excellent accuracy, it still consumes too much time and too many resources in terms of data processing. To improve the dilemma, in this study, modified multi-scale symbolic dynamic entropy (MMSDE) is adopted to identify the damaged location of the civil structure. A damage index (DI) based on the entropy diagram is also proposed to clearly indicate the damage location. A seven-story numerical model was created to verify the efficiency of the proposed SHM system. The results of the analysis of each case of damage show that the MMSDE curve for the damaged floor is lower than that for the healthy floor, and the structural damage can be correctly diagnosed by the damage index. Subsequently, a scaled-down steel benchmark experiment, including 15 damage cases, was conducted to verify the practical performance of the SHM system. The confusion matrix was used to further evaluate the SHM system. The results demonstrated that the MMSD-based system can quickly diagnose structural safety with reliability and accuracy. It can be used in the field of long-term structural health monitoring in the near future.

7.
Sensors (Basel) ; 21(14)2021 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-34300646

RESUMO

This paper proposes a novel broadband octagonal patch antenna with parasitic patches. The proposed patch antenna is constructed with four parasitic patches around a central radiating octagonal element. It is illustrated that this arrangement can be used to improve the antenna bandwidth and gain when compared with that of conventional antennas. The proposed patch antenna is very simple, low-profile, and economical. The typical analysis of the proposed antenna is analyzed by the S11(S-parameter), the radiation pattern, and the realized gain. It can achieve an impedance bandwidth of 1.44 GHz and a high gain of 8.56 dBi in the 8.5 GHz band. Furthermore, the proposed antenna shows that the directional pattern and HPBW measurement results of E and H-plane were 70° and 74° at 8.5 GHz, and 74° and 83° at 9 GHz, and 47° and 42° at 9.5 GHz, respectively.

8.
Sensors (Basel) ; 21(22)2021 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-34833823

RESUMO

The indoor application of wave propagation in the 5G network is essential to fulfill the increasing demands of network access in an indoor environment. This study investigated the wave propagation properties of line-of-sight (LOS) links at two long corridors of Chosun University (CU). We chose wave propagation measurements at 3.7 and 28 GHz, since 3.7 GHz is the closest to the roll-out frequency band of 3.5 GHz in South Korea and 28 GHz is next allocated frequency band for Korean telcos. In addition, 28 GHz is the promising millimeter band adopted by the Federal Communications Commission (FCC) for the 5G network. Thus, the 5G network can use 3.7 and 28 GHz frequencies to achieve the spectrum required for its roll-out frequency band. The results observed were applied to simulate the path loss of the LOS links at extended indoor corridor environments. The minimum mean square error (MMSE) approach was used to evaluate the distance and frequency-dependent optimized coefficients of the close-in (CI) model with a frequency-weighted path loss exponent (CIF), floating-intercept (FI), and alpha-beta-gamma (ABG) models. The outcome shows that the large-scale FI and CI models fitted the measured results at 3.7 and 28 GHz.

9.
Sensors (Basel) ; 21(18)2021 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-34577298

RESUMO

This paper describes a novel feed system for compact, wideband, high gain six-slot Vivaldi antenna arrays on a single substrate layer using a unique combination of power splitters based on binary T-junction power splitter topology, frequency-independent phase shifter, and a T-branch. The proposed antenna system consists of six Vivaldi antennas, three on the left, and three on the right arm. Each arm connects with T-junction power divider splitter topology, given that the right arm is linked through a frequency-independent phase shifter. Phase shifters ensure that the beam is symmetrical without splitting in a radiating plane so that highly directive radiation patterns occur. The optimal return losses (S-parameters) are well enriched by reforming Vivaldi's feeding arms and optimizing Vivaldi slots and feeds. A novel feature of our design is that the antenna exhibits the arrangements of a T-junction power splitter with an out-of-phase feeding mechanism in one of the arms, followed by a T-branching feeding to even arrays of proper Vivaldi antenna arrangement contributing high realized gain and front-to-back ratio up to 14.12 dBi and 23.23 dB respectively applicable for not only ultra-wideband (UWB) application, also for sensing and position detecting. The high directivity over the entire UWB frequency band in both higher and lower frequency ranges ensures that the antenna can be used in microwave through-wall imaging along with resolution imaging for ground penetration radar (GPR) applications. The fabricated antenna parameters are in close agreement with the simulated and measured results and are deployed for the detection of targets inside the voids of the concrete brick.


Assuntos
Micro-Ondas , Radar , Comunicação , Diagnóstico por Imagem , Testes Diagnósticos de Rotina
10.
Sensors (Basel) ; 21(4)2021 Feb 09.
Artigo em Inglês | MEDLINE | ID: mdl-33572178

RESUMO

Millimeter-wave (30-300 GHz) frequency is a promising candidate for 5G and beyond wireless networks, but atmospheric elements limit radio links at this frequency band. Rainfall is the significant atmospheric element that causes attenuation in the propagated wave, which needs to estimate for the proper operation of fade mitigation technique (FMT). Many models have been proposed in the literature to estimate rain attenuation. Various models have a distinct set of input parameters along with separate estimation mechanisms. This survey has garnered multiple techniques that can generate input dataset for the rain attenuation models. This study extensively investigates the existing terrestrial rain attenuation models. There is no survey of terrestrial rain mitigation models to the best of our knowledge. In this article, the requirements of this survey are first discussed, with various dataset developing techniques. The terrestrial links models are classified, and subsequently, qualitative and quantitative analyses among these terrestrial rain attenuation models are tabulated. Also, a set of error performance evaluation techniques is introduced. Moreover, there is a discussion of open research problems and challenges, especially the exigency for developing a rain attenuation model for the short-ranged link in the E-band for 5G and beyond networks.

11.
Entropy (Basel) ; 23(1)2020 Dec 31.
Artigo em Inglês | MEDLINE | ID: mdl-33396377

RESUMO

This study proposesd a novel, entropy-based structural health monitoring (SHM) system for measuring microvibration signals generated by actual buildings. A structural health diagnosis interface was established for demonstration purposes. To enhance the reliability and accuracy of entropy evaluation at various scales, composite multiscale cross-sample entropy (CMSCE) was adopted to increase the number of coarse-grained time series. The degree of similarity and asynchrony between ambient vibration signals measured on adjacent floors was used as an in-dicator for structural health assessment. A residential building that has been monitored since 1994 was selected for long-term monitoring. The accumulated database, including both the earthquake and ambient vibrations in each seismic event, provided the possibility to evaluate the practicability of the CMSCE-based method. Entropy curves obtained for each of the years, as well as the stable trend of the corresponding damage index (DI) graphs, demonstrated the relia-bility of the proposed SHM system. Moreover, two large earthquake events that occurred near the monitoring site were analyzed. The results revealed that the entropy values may have been slightly increased after the earthquakes. Positive DI values were obtained for higher floors, which could provide an early warning of structural instability. The proposed SHM system is highly stable and practical.

12.
FASEB J ; 32(2): 654-668, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-28970251

RESUMO

Administration of exosomes derived from mesenchymal stromal cells (MSCs) could improve some neurologic conditions by transferring functional biomolecules to recipient cells. Furthermore, exosomes from hypoxic progenitor cells exerted better therapeutic effects in organ injury through specific cargoes. However, there are no related reports about whether exosomes derived from MSCs or hypoxia-preconditioned MSCs (PC-MSCs) could prevent memory deficits in Alzheimer disease (AD). In this study, the exosomes derived from MSCs or PC-MSCs were systemically administered to transgenic APP/PS1 mice. The expression of miR-21 in MSCs was significantly increased after hypoxic treatment. Injection of exosomes from normoxic MSCs could rescue cognition and memory impairment according to results of the Morris water maze test, reduced plaque deposition, and Aß levels in the brain; could decrease the activation of astrocytes and microglia; could down-regulate proinflammatory cytokines (TNF-α and IL-1ß); and could up-regulate anti-inflammatory cytokines (IL-4 and -10) in AD mice, as well as reduce the activation of signal transducer and activator of transcription 3 (STAT3) and NF-κB. Compared to the group administered exosomes from normoxic MSCs, in the group administered exosomes from PC-MSCs, learning and memory capabilities were significantly improved; the plaque deposition and Aß levels were lower, and expression of growth-associated protein 43, synapsin 1, and IL-10 was increased; and the levels of glial fibrillary acidic protein, ionized calcium-binding adaptor molecule 1, TNF-α, IL-1ß, and activation of STAT3 and NF-κB were sharply decreased. More importantly, exosomes from PC-MSCs effectively increased the level of miR-21 in the brain of AD mice. Additionally, replenishment of miR-21 restored the cognitive deficits in APP/PS1 mice and prevented pathologic features. Taken together, these findings suggest that exosomes from PC-MSCs could improve the learning and memory capabilities of APP/PS1 mice, and that the underlying mechanism may lie in the restoration of synaptic dysfunction and regulation of inflammatory responses through regulation of miR-21.-Cui, G.-H., Wu, J., Mou, F.-F., Xie, W.-H., Wang, F.-B., Wang, Q.-L., Fang, J., Xu, Y.-W., Dong, Y.-R., Liu, J.-R., Guo, H.-D. Exosomes derived from hypoxia-preconditioned mesenchymal stromal cells ameliorate cognitive decline by rescuing synaptic dysfunction and regulating inflammatory responses in APP/PS1 mice.


Assuntos
Doença de Alzheimer/metabolismo , Encéfalo/metabolismo , Disfunção Cognitiva/metabolismo , Exossomos/metabolismo , Precondicionamento Isquêmico , Células-Tronco Mesenquimais/metabolismo , Sinapses/metabolismo , Doença de Alzheimer/patologia , Animais , Encéfalo/patologia , Disfunção Cognitiva/patologia , Citocinas/metabolismo , Exossomos/patologia , Células-Tronco Mesenquimais/patologia , Camundongos , Camundongos Transgênicos , Sinapses/patologia
13.
Immun Ageing ; 16: 10, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31114624

RESUMO

BACKGROUND: Exosomes are lipid-bilayer enclosed nano-sized vesicles that transfer functional cellular proteins, mRNA and miRNAs. Mesenchymal stem cells (MSCs) derived exosomes have been demonstrated to prevent memory deficits in the animal model of Alzheimer's disease (AD). However, the intravenously injected exosomes could be abundantly tracked in other organs except for the targeted regions in the brain. Here, we proposed the use of central nervous system-specific rabies viral glycoprotein (RVG) peptide to target intravenously-infused exosomes derived from MSCs (MSC-Exo) to the brain of transgenic APP/PS1 mice. MSC-Exo were conjugated with RVG through a DOPE-NHS linker. RESULTS: RVG-tagged MSC-Exo exhibited improved targeting to the cortex and hippocampus after being administered intravenously. Compared with the group administered MSC-Exo, in the group administered RVG-conjugated MSC-Exo (MSC-RVG-Exo) plaque deposition and Aß levels were sharply decreased and activation of astrocytes was obviously reduced. The brain targeted exosomes derived from MSCs was better than unmodified exosomes to improve cognitive function in APP/PS1 mice according to Morris water maze test. Additionally, although MSC-Exo injected intravenously reduced the expression of pro-inflammatory mediators TNF-α, IL-ß, and IL-6, but the changes of anti-inflammatory factors IL-10 and IL-13 were not obvious. However, administration of MSC-RVG-Exo significantly reduced the levels of TNF-α, IL-ß, and IL-6 while significantly raised the levels of IL-10, IL-4 and IL-13. CONCLUSIONS: Taken together, our results demonstrated a novel method for increasing delivery of exosomes for treatment of AD. By targeting exosomes to the cortex and hippocampus of AD mouse, there was a significant improvement in learning and memory capabilities with reduced plaque deposition and Aß levels, and normalized levels of inflammatory cytokines.

14.
Sensors (Basel) ; 19(14)2019 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-31295899

RESUMO

The single antenna used in conventional ultra-wideband radar has difficulty tracking targets over a wide range because of a relatively narrow beamwidth. Herein, we propose a beamforming antenna that can track targets over a wide range by electronically controlling the main beam of the antenna. The proposed beamforming antenna was fabricated by connecting a 1 × 4 linear array antenna and a 4 × 4 Butler matrix. The Butler matrix was fabricated in a laminated substrate using two TRF-45 substrates. Furthermore, the input Ports 1-4 generate a phase difference at regular intervals in each output port, and the output phase is fed to the array antenna. The proposed tapered-slot antenna was fabricated on a Taconic TLY substrate, and the impedance bandwidth of the antenna was achieved within a wide bandwidth of 4.32 GHz by satisfying a VSWR (Voltage Standing Wave Ratio) ≤2 within the 1.45-5.78 GHz band. Furthermore, the fabricated antenna has directional radiation patterns, which was found to be a suitable characsteristic for location tracking in a certain direction. Finally, the beamforming antenna has four beamforming angles, and to verify the performance for an indoor location tracking application of impulse-radio ultra-wideband radar, it was connected to an NVA-R661 module.

15.
Sensors (Basel) ; 18(9)2018 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-30189629

RESUMO

A lightweight composite bridge deck system composed of steel orthotropic deck stiffened with thin Ultra-High Performance Concrete (UHPC) layer has been proposed to eliminate fatigue cracks in orthotropic steel decks. The debonding between steel deck and UHPC layer may be introduced during construction and operation phases, which could cause adverse consequences, such as crack-induced water invasion and distinct reduction of the shear resistance. The piezoelectric lead zirconate titanate (PZT)-based technologies are used to detect interfacial debonding defects between the steel deck and the UHPC layer. Both impedance analysis and wave propagation method are employed to extract debonding features of the steel-UHPC composite slab with debonding defect in different sizes and thicknesses. Experimental tests are performed on two steel-UHPC composite slabs and a conventional steel-concrete composite deck. Additionally, an improved Particle Swarm Optimization (PSO)-k-means clustering algorithm is adopted to obtain debonding patterns based on the feature data set. The laboratory tests demonstrate that the proposed approach provides an effective way to detect interfacial debonding of steel-UHPC composite deck.

16.
Sensors (Basel) ; 18(12)2018 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-30513953

RESUMO

In this paper, a quasi-Yagi antenna for an indoor location tracking system is proposed. The performance of the proposed antenna was verified by testing it using an indoor location tracking system. To improve the bandwidth and gain, two parasitic directors were added near the dipole. The performance verification of the proposed antenna is explained, along with a performance comparison of the VSWR (voltage standing wave ratio) radiation pattern and the realized gain. The proposed antenna was connected to an NVA-R661 module of Xethru Inc. for indoor location tracking. The proposed antenna exhibited a wide bandwidth of 4.36 GHz by satisfying a VSWR ≤ 2 from 5.03 to 9.39 GHz, the maximum gain was 6.46 dBi in the 8 GHz band. The radiation pattern exhibited a good directivity characteristic within the proposed band. The location tracking result of a moving target clearly describes the route of the target along a moving line.

17.
Neurol Sci ; 37(8): 1241-6, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27071688

RESUMO

As possible candidate screening instruments for benign paroxysmal positional vertigo (BPPV), studies to validate the Dizziness Handicap Inventory (DHI) sub-scale (5-item and 2-item) and total scores are rare in China. From May 2014 to December 2014, 108(55 with and 53 without BPPV) patients complaining of episodic vertigo in the past week from a vertigo outpatient clinic were enrolled for DHI evaluation, as well as demographic and other clinical data. Objective BPPV was subsequently determined by positional evoking maneuvers under the record of optical Frenzel glasses. Cronbach's coefficient α was used to evaluate the reliability of psychometric scales. The validity of DHI total, 5-item and 2-item questionnaires to screen for BPPV was assessed by receiver operating characteristic (ROC) curves. It revealed that the DHI 5-item questionnaire had good internal consistency (Cronbach's coefficient α = 0.72). Area under the curve of total DHI, 5-item and 2-item scores for discriminating BPPV from those without was 0.678 (95 % CI 0.578-0.778), 0.873(95 % CI 0.807-0.940) and 0.895(95 % CI 0.836-0.953), respectively. It revealed 74.5 % sensitivity and 88.7 % specificity in separating BPPV and those without, with a cutoff value of 12 in the 5-item questionnaire. The corresponding rate of sensitivity and specificity was 78.2 and 88.7 %, respectively, with a cutoff value of 6 in 2-item questionnaire. The present study indicated that both 5-item and 2-item questionnaires in the Chinese version of DHI may be more valid than DHI total score for screening objective BPPV and merit further application in clinical practice in China.


Assuntos
Vertigem Posicional Paroxística Benigna/complicações , Vertigem Posicional Paroxística Benigna/diagnóstico , Tontura/diagnóstico , Tontura/etiologia , Inquéritos e Questionários , Tradução , Adulto , Idoso , China , Avaliação da Deficiência , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Curva ROC , Reprodutibilidade dos Testes , Estudos Retrospectivos
18.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 46(1): 35-41, 2015 Jan.
Artigo em Zh | MEDLINE | ID: mdl-25807793

RESUMO

OBJECTIVE: To investigate the effects of kriippel-like factor 5 (KLF5) overexpression on gemcitabine (GEME)-induced lung adenocarcinoma cell apoptosis and its molecular mechanisms. METHODS: Lung adenocarcinoma cell line H441 were transfected with KLF5 plasmid or control plasmid. 68 h later, cells were treated with 100 nmol/L GEME for 4 h, then cell number counting and flow cytometry were applied to detect cell proliferation and apoptosis; Western blot were used to analyse the expression level of KLF5, RT-PCR were used to analyse the KLF5 gene, apoptosis-related genes CD95 and BAX; Immunofluorescence staining was performed to detect the expression level of apoptosis-related protein Caspase 3. RESULTS: The overexpressions of KLF5 protein were detected in cultured-lung adenocarcinoma cell line H441 cells when KLF5 plasmids were transfected 68 h. Further flow cytometry, overexpression of KLF5 in H441 cell line affected the biological process of apoptosis significantly. No significant changes of apoptosis and expression level of CD95 and BAX in H441 cells were observed by KLF5 overexpression without GEME treatment (P>0.05). Under GEME induction, the proportion of apoptotic cells and expression level of CD95 and BAX were increased significantly in H441 cells by KLF5 overexpression, compared with that of control (P<0.05); The overexpression of KLF5 restrained the proliferation of H441 cells; Immunofluorescence staining of Caspase 3 was also enhanced after KLF5 overexpression. CONCLUSION: Under GEME induction, the overexpression of KLF5 promoted the apoptosis of lung adenocarcinoma cell line H441 in vitro, possibly through the inhibition of cell proliferation and repair/activation of apoptosis pathway proteins, such as Caspase 3, CD95 and BAX.


Assuntos
Adenocarcinoma/patologia , Apoptose , Desoxicitidina/análogos & derivados , Fatores de Transcrição Kruppel-Like/metabolismo , Neoplasias Pulmonares/patologia , Adenocarcinoma/metabolismo , Adenocarcinoma de Pulmão , Caspase 3/metabolismo , Linhagem Celular Tumoral/efeitos dos fármacos , Proliferação de Células , Desoxicitidina/farmacologia , Humanos , Neoplasias Pulmonares/metabolismo , Transfecção , Proteína X Associada a bcl-2/metabolismo , Receptor fas/metabolismo , Gencitabina
19.
Guang Pu Xue Yu Guang Pu Fen Xi ; 34(4): 894-7, 2014 Apr.
Artigo em Zh | MEDLINE | ID: mdl-25007594

RESUMO

In the present paper, an innovative experiment was done. The authors measured the Raman spectra of CS2 mixed with THF at different volume fractions. According to the J. F. Bertran theory, we analyzed the changing regularity of v1-2v2 Fermi resonance along with the different concentration. It was shown that the characteristic parameters of Fermi resonance in solution, such as spectra intensity, frequency space, coupling coefficient and anharmonic constant, will make changes along with solution concentration variation. Because of the existing of solvent effects, the spectra intensity decreases gradually, and the coupling coefficient increases gradually. The explanation of such changes is that the vibration spectra are affected not only by scattering coefficient, but also by more weak hydrogen bond's formation. In addition, the asymmetric frequency shift of the Raman spectra was also found in the paper. The fundamental frequency v1 basically has no shifts, but the overtone frequency 2v2 moves towards the high wave number gradually, which is inconsistent with the theory of symmetrical movement. According to the study of molecular microcosmic action, the formation and mechanism of the weak hydrogen bond can preferably explain the above phenomenon. The research has a major influence on the further study of solvent effects in Fermi resonance. And the paper will also provide certain reference value for the study of molecule vibration spectra in solution.

20.
Sci Rep ; 14(1): 8262, 2024 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-38594427

RESUMO

In this paper, a wideband polarization conversion metasurface is designed. Additionally, coding and chessboard metasurfaces are specifically tailored for radar cross-section reduction (RCS). Initially, a compact unit cell demonstrating exceptional polarization conversion performance is introduced, achieving a polarization conversion ratio (PCR) exceeding 90 % across frequencies ranging from 7.9 to 22.7 GHz. The PCR remains effective even when oblique incidence angles of up to 30 ∘ are utilized across this frequency band. Roger RT5880, with a thickness of 0.254 mm, serves as the substrate. An airgap is introduced between the substrate and the ground plane to enhance the polarization conversion bandwidth. This unit cell serves as the fundamental building block for subsequent metasurface configurations. To assess the scalability and effectiveness, a 36 × 36 unit array is assembled, confirming efficient polarization conversion capabilities extending to larger structures. Moreover, a 1-bit coding unit "0" and "1" are formed by the Pancharatnam-Berry phase based on the same-sized meta-atom with 90 ∘ orientations. The robustness and practicality of the design are demonstrated by creating 12 × 12 lattices and evaluating their RCS reduction potential under two distinct scenarios: a chessboard pattern and a coding-based scheme. Notably, its results indicate substantial RCS reduction across a broad frequency spectrum (7.9 to 22.7 GHz) for both configurations. This study demonstrates the wide-ranging applicability of metasurface design, making it a valuable contribution to the fields of microwave engineering, polarization control, and radar stealth technology. Owing to its simplicity, bandwidth, and versatility, this approach offers innovative solutions for diverse real-world applications.

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