RESUMO
BACKGROUND AND AIM: SIRT1 and PGC1α are two important genes, which play critical roles in regulating oxidative stress and inflammation processes. The study aimed assess the effects of coadministration of omega-3 and vitamin E supplements on SIRT1 and PGC1α gene expression and serum levels of antioxidant enzymes in coronary artery disease (CAD) patients. METHODS AND RESULTS: Participants of this randomized controlled trial included 60 CAD male patients who were categorized into three groups: Group 1 received omega-3 (4 g/day) and vitamin E placebo (OP), group 2 omega-3 (4 g/day) and vitamin E (400 IU/day; OE), and group 3 omega-3 and vitamin E placebos (PP) for 2 months. Gene expression of SIRT1 and PGC1α in peripheral blood mononuclear cells (PBMCS) was assessed by reverse transcription polymerase chain reaction (RT-PCR). Furthermore, serum antioxidant enzyme and high-sensitivity C-reactive protein (hsCRP) levels were assessed at the beginning and end of the intervention. Gene expression of SIRT1 and PGC1α increased significantly in the OE group (P = 0.039 and P = 0.050, respectively). Catalase and hsCRP levels increased significantly in the OE and OP groups. However, glutathione peroxidase (GPX) and superoxide dismutase (SOD) levels did not statistically change in all groups. The total antioxidant capacity (TAC) increased significantly in the OE group (P = 0.009) but not in OP and PP groups. CONCLUSION: Supplementation of omega-3 fatty acids in combination with vitamin E may have beneficial effects on CAD patients by increasing gene expression of SIRT1 and PGC1α and improving oxidative stress and inflammation in these patients.
Assuntos
Antioxidantes/metabolismo , Catalase/sangue , Doença da Artéria Coronariana/tratamento farmacológico , Estenose Coronária/tratamento farmacológico , Suplementos Nutricionais , Ácidos Docosa-Hexaenoicos/administração & dosagem , Ácido Eicosapentaenoico/administração & dosagem , Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo/sangue , Sirtuína 1/sangue , Vitamina E/administração & dosagem , Biomarcadores/sangue , Proteína C-Reativa/metabolismo , Angiografia Coronária , Doença da Artéria Coronariana/sangue , Doença da Artéria Coronariana/diagnóstico por imagem , Doença da Artéria Coronariana/enzimologia , Estenose Coronária/sangue , Estenose Coronária/diagnóstico por imagem , Estenose Coronária/enzimologia , Suplementos Nutricionais/efeitos adversos , Ácidos Docosa-Hexaenoicos/efeitos adversos , Método Duplo-Cego , Ácido Eicosapentaenoico/efeitos adversos , Glutationa Peroxidase/sangue , Nível de Saúde , Humanos , Mediadores da Inflamação/sangue , Irã (Geográfico) , Masculino , Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo/genética , Sirtuína 1/genética , Superóxido Dismutase/sangue , Terapêutica , Fatores de Tempo , Regulação para Cima , Vitamina E/efeitos adversosRESUMO
BACKGROUND: Pemphigus vulgaris (PV) is an autoimmune blistering disorder of the skin and/or mucosa. Increased levels of reactive oxygen species (ROS) were previously reported in PV. AIM: Because oxidative stress has an important role in the inflammatory process, we designed this study to evaluate the antioxidant status in patients with PV and to compare it with that of healthy controls (HCs). METHODS: In this case-control study, 43 newly diagnosed patients with PV were compared with 58 HCs. The severity of the disease was estimated according to Harman scores. Erythrocyte glutathione peroxidase (GPx), superoxide dismutase (SOD), CAT and serum malondialdehyde (MDA) activities and total antioxidant capacity were measured. Data were analyzed by independent t-test. RESULTS: Both groups were similar in sex, age and body mass index. Mean duration of disease was 5.6 months. Mean oral and skin severities were 1.79 and 2.3 respectively, based on Harman scores. SOD activity was not significantly different between groups (1003.30 ± 39.96 vs. 1009.76 ± 32.68 U/gHb). Levels were noticeably higher in patients with PV than in HCs for both GPx (52.13 ± 2.85 vs. 36.63 ± 1.49 U/gHb, respectively; P < 0.001) and CAT (205.69 ± 8.10 vs. 130.26 ± 6.80 kU/gHb, respectively; P < 0.001) activities, and CAT activity correlated with disease severity. In addition, patients had lower total antioxidant capacity than controls (3.39 ± 0.06 vs. 3.72 ± 0.09 mmol/L, P = 0.006). There was no noticeable difference in serum MDA between the two groups (P = 0.45). CONCLUSIONS: Patients with PV have significantly higher antioxidant enzyme activities and lower total antioxidant capacity compared with HCs. These data indicate the importance of improving antioxidant level in patients with pemphigus.
Assuntos
Antioxidantes/metabolismo , Pênfigo/enzimologia , Adolescente , Adulto , Idoso , Estudos de Casos e Controles , Catalase/metabolismo , Feminino , Glutationa Peroxidase/metabolismo , Humanos , Masculino , Malondialdeído/metabolismo , Pessoa de Meia-Idade , Estresse Oxidativo/fisiologia , Pênfigo/metabolismo , Superóxido Dismutase/metabolismo , Adulto JovemRESUMO
This study was designed to find out Ruta graveolens L. functional components, which have immobilisation effect on human spermatozoa for contraceptive use. A five-step fractionation method was used to derive different components from rue aqueous extract by using hexane, chloroform, ethanol, acetone and ultrapure water. Gas chromatography-mass spectrophotometery (GC-MS) of all fractions and the aqueous extract were performed to determine the chemical components. The immobilisation assay and membrane integrity test were also performed with four different coumarins, which were found in GC-MS in a concentration of 10 µm. Hexane, chloroform, acetone and ethanol fractions could significantly decrease motility of sperms within the first and the second hours. Hexane fraction had also significant immediate effect. The aqueous fraction had no effect on sperm motility. Meanwhile, GC-MS revealed that aqueous extract and effective fractions had similar coumarin compounds. We performed the immobilisation assay on four different coumarins, which were found in GC-MS in a concentration of 10 µm. Reduction of sperm motility was only significant for xanthotoxin. In the sperm viability and membrane integrity tests, hexane and ethanolic fractions could impair sperm vitality significantly, in contrast to coumarins. These results indicated that a part of immobilising effect of rue could be due to its coumarins. The possible mechanism could be blocking of spermatozoa potassium channels.
Assuntos
Cumarínicos/farmacologia , Extratos Vegetais/farmacologia , Ruta , Motilidade dos Espermatozoides/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Cumarínicos/análise , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Masculino , Extratos Vegetais/químicaRESUMO
BACKGROUND: Hypovitaminosis D, especially during cold seasons, is quite prevalent among primary school children in Tehran. The present study aimed to compare the efficacy of calcium-vitamin D-fortified-milk, -orange juice and supplement in primary school children. METHODS: Children aged 9-12 years from both sexes were randomly assigned to one of six groups to receive plain milk, fortified milk, plain orange juice, fortified orange juice, supplement or placebo. Both fortified-milk and -juice contained 100 IU of vitamin D and 500 mg of calcium per 200 mL package, whereas supplement contained 200 IU of vitamin D and 500 mg of calcium. The duration of intervention was 12 weeks, from November 2008 to March 2009. RESULTS: A total of 410 children completed the study. Regression analysis with adjustment for both sex and initial values of 25(OH)D revealed that consumption of either supplement, fortified orange juice or fortified milk resulted in a 20.8 nm [confidence interval (CI) = 17.4-23.9], 9.9 nm (CI = 7.4-12.3) or 6.9 nm (CI = 3.3-10.5) increase in circulating 25(OH)D compared to the related control groups. However, changes in serum osteocalcin and intact parathyroid hormone in the supplement group did not differ from those of fortified milk or plain milk. The increment of osteocalcin in both plain milk and fortified milk was more than in fortified orange juice, although the difference was statistically insignificant. CONCLUSIONS: Despite having double amount of vitamin D and being more effective in raising serum 25(OH)D, supplementation did not confer additional benefit in terms of osteocalcin and intact parathyroid hormone compared to either fortified or plain milk.
Assuntos
Bebidas , Osso e Ossos , Cálcio da Dieta/administração & dosagem , Suplementos Nutricionais , Alimentos Fortificados , Estado Nutricional , Vitamina D/administração & dosagem , Animais , Biomarcadores/sangue , Cálcio da Dieta/farmacologia , Criança , Colecalciferol/sangue , Citrus sinensis , Feminino , Preferências Alimentares , Humanos , Masculino , Leite , Osteocalcina/sangue , Hormônio Paratireóideo/sangue , Oligoelementos/administração & dosagem , Oligoelementos/farmacologia , Vitamina D/sangue , Vitamina D/farmacologia , Deficiência de Vitamina D/sangue , Deficiência de Vitamina D/prevenção & controle , Vitaminas/administração & dosagem , Vitaminas/sangue , Vitaminas/farmacologiaRESUMO
BACKGROUND: Obesity is associated with lowgrade systemic inflammation which has been linked to the increased risk of cardiovascular disease and Type 2 diabetes in obese patients. AIM: To evaluate changes in pro/anti-inflammatory adipocytokines and metabolic profile after moderate diet-induced weight loss. SUBJECTS AND METHODS: Twenty-nine pre-menopausal obese women (body mass index ≥30 kg/m2) aged 21 to 54 years without diabetes, hypertension, or hyperlipidemia, were enrolled in this study. We measured anthropometric parameters, lipid and glucose profiles, interleukin (IL)-6, IL-10, and IL-18 in obese women, who then entered a medically supervised program aimed at reducing body weight by 10% or more. Obese women restricted their caloric intake (by 500-1000 kcal/day) and consumed 50 g/day of a fiber supplement (Slim Last Powder) for 12 weeks. RESULTS: By completing the dietary intervention program, weight (Δ = -10.0%, p<0.0001), body mass index, waist circumference, triceps skinfold thickness, total cholesterol, triglyceride, and fasting plasma glucose significantly decreased, while HDL-cholesterol significantly increased. While plasma levels of IL-6 and IL-18 decreased by 27% after 12 weeks, no significant change was observed in circulating levels of IL-10. CONCLUSION: Our study suggests that an improved body composition induced by restriction of energy intake is associated with favorable serum concentrations of IL-6 and IL-18 in obese women. However, the anti-inflammatory IL-10 is not affected by a moderate weight decrease.
Assuntos
Citocinas/sangue , Dieta Redutora , Obesidade/sangue , Obesidade/dietoterapia , Redução de Peso/fisiologia , Adulto , Citocinas/metabolismo , Dieta Redutora/métodos , Feminino , Humanos , Mediadores da Inflamação/sangue , Interleucina-10/sangue , Interleucina-18/sangue , Interleucina-6/sangue , Pessoa de Meia-Idade , Obesidade/imunologia , Triglicerídeos/sangue , Redução de Peso/imunologia , Adulto JovemRESUMO
AIM: Increased oxidative stress and impaired antioxidant defense contribute to pathogenesis and progression of type 2 diabetes. Consistent with this fact, it has been shown that diabetic patients have reduced coenzyme Q10 level. In this study we sought to compare the effect of coenzyme Q10 versus placebo on glycemic control and lipid profile in type 2 diabetic patients. METHODS: In a randomized double-blind placebo-controlled trial, 64 type 2 diabetic patients were randomly assigned to receive either 200 mg Q10 or placebo daily for 12 weeks. Fasting blood samples were obtained and fasting plasma glucose (FPG), HbA1c, total cholesterol (TC), triglycerides (TG), LDL-C and HDL-C were measured. RESULTS: In this study no significant differences considering age, body mass index (BMI), diabetes duration, FPG, HbA1c, TC, TG, LDL-C and HDL-C were shown between two groups. Serum HbA1C concentration decreased in the Q10 treated group (8 ± 2.28 vs. 8.61 ± 2.47%) with no significant effect in the placebo group. Following intervention no differences have been shown regarding FPG, TG and HDL-C in Q10 treated group. Furthermore, mean differences of TC and LDL-C level were statistically altered between two groups (P value=0.027 and 0.039 respectively). CONCLUSION: In this study, Q10 treatment improved glycemic control, total and LDL cholesterol but these differences were associated with no favourable effects on TG and HDL-C.
Assuntos
Diabetes Mellitus Tipo 2/tratamento farmacológico , Diabetes Mellitus Tipo 2/metabolismo , Ubiquinona/análogos & derivados , Método Duplo-Cego , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Ubiquinona/uso terapêuticoRESUMO
BACKGROUND: Vitamin D status has been linked to both T helper (Th)1/Th2 balance and susceptibility to type 1 diabetes (T1D). The present study aimed to evaluate vitamin D status and its relation to Th1/Th2 balance in subjects with T1D, their siblings and unrelated healthy controls during autumn and winter 2008-2009. METHODS: A case-control study was conducted on subjects with T1D (n(1) = 60) and two control groups comprising nondiabetic siblings (n(2) = 60) and unrelated healthy controls (n(3) = 60). Assessments of dietary intake, anthropometry, intact parathyroid hormone (iPTH) and 25(OH)D were performed. Serum levels of immunoglobulin (Ig)G(2) and IgE, as well as the IgG2/IgE ratio, were used to evaluate Th1/Th2 balance. Vitamin D status was defined based on circulating 25(OH)D as deficiency: <27.5 nm; insufficiency 27.5 ≤ 25(OH)D <50 nm; and sufficiency ≥50 nm. RESULTS: Vitamin D status did not differ significantly among the groups. Similarly, no significant difference in 25(OH)D, iPTH, IgG(2), IgE and IgG(2)/IgE was found. In multiple regression analysis of pooled data, PTH and body mass index were the predictors of IgG(2)/IgE. In the diabetic group, both PTH and age and, in siblings, PTH only, were the predictors of IgG(2)/IgE ratio. CONCLUSIONS: These data suggest PTH as the major predictor of immune deviance towards the Th1 response in both type 1 diabetic subjects and their siblings. Considering that the active form of vitamin D suppresses PTH production, it is hypothesised that vitamin D replenishment of just those who are genetically prone to the disease (i.e. siblings) may be regarded as a preventive measure against T1D.
Assuntos
Diabetes Mellitus Tipo 1/imunologia , Imunoglobulinas/sangue , Estado Nutricional , Linfócitos T Auxiliares-Indutores/imunologia , Deficiência de Vitamina D/diagnóstico , Vitamina D/análogos & derivados , Adolescente , Adulto , Índice de Massa Corporal , Estudos de Casos e Controles , Criança , Pré-Escolar , Diabetes Mellitus Tipo 1/sangue , Ingestão de Energia , Feminino , Humanos , Imunoglobulina E/sangue , Imunoglobulina G/sangue , Irã (Geográfico) , Masculino , Hormônio Paratireóideo/sangue , Células Th1/imunologia , Células Th2/imunologia , Vitamina D/sangue , Deficiência de Vitamina D/sangue , Deficiência de Vitamina D/imunologiaRESUMO
This study aimed to carry out a malaria situation analysis, species composition and susceptibility levels of the main malaria vector, Anopheles stephensi, to different insecticides in Bashagard. A longitudinal survey was conducted in 2 randomly selected villages in Bashagard. Malaria vectors were sampled by dipping method for the larvae and hand catch, night-biting catch, total catch, and shelter pit collection for the adults. Standard WHO susceptibility tests were used for a variety of insecticides on F1 progeny of An. stephensi reared from wild-caught females. In total, 693 adult anopheline mosquitoes and 839 third and fourth-instar larvae were collected and identified. They comprised 7 species; the most abundant adult and larvae anopheline mosquito was An. dthali (40.7% and 30.5% respectively). An. culicifacies (24.2%) and An. stephensi (16.7%) were the next most common species for adult mosquitoes. An. stephensi was fully susceptible to malathion and pyrethroid insecticides but resistant to DDT and tolerant to dieldrin.
Assuntos
Anopheles/efeitos dos fármacos , Anopheles/parasitologia , Insetos Vetores/efeitos dos fármacos , Insetos Vetores/parasitologia , Inseticidas/farmacologia , Malária/epidemiologia , Animais , Relação Dose-Resposta a Droga , Feminino , Humanos , Resistência a Inseticidas , Irã (Geográfico)/epidemiologia , Larva , Malária/prevenção & controle , Masculino , Técnicas Microbiológicas , Controle de MosquitosRESUMO
Iran is in the malaria pre-elimination stage. In this situation community based strategies are important for malaria control. In order to intensify elimination activities we need to have a better understanding of the knowledge, attitudes and practices (KAP) of malaria prevention in the community. We carried out a KAP study in one malaria endemic district in southern Iran in 2009 using a structured questionnaire. Five villages with indigenous malaria cases during the previous year and a high potential for anopheline mosquito breeding were selected. All the households in each village were visited and the head of the household or other responsible adult was interviewed. Data were analyzed using SPSS 11.5. A total of 494 respondents from the five villages participated. More than 33% of people in the study area were living in sheds with poor facilities. The illiteracy level of the studied population was high (44.2%) and significantly affected the knowledge and practices of the respondents about malaria control (p<0.05). Descriptive analysis showed significant differences between sex, job and history of malaria infection of the respondents and their knowledge and practices about malaria control (p<0.05). Knowledge of the respondents about the route of transmission in malaria was good; 72.1% knew mosquitoes were the vector. Most people knew at least one symptom of the disease. The practices of respondents were 59.5% for bednet use and 9.3% for screening windows. The knowledge, attitudes and practices of respondents in this survey about malaria control were good in some cases, but in general needed to be improved with educational programs.
Assuntos
Doenças Endêmicas/prevenção & controle , Conhecimentos, Atitudes e Prática em Saúde , Malária/epidemiologia , Malária/prevenção & controle , Animais , Estudos Transversais , Culicidae , Feminino , Habitação/estatística & dados numéricos , Humanos , Insetos Vetores , Irã (Geográfico)/epidemiologia , Masculino , Mosquiteiros/estatística & dados numéricos , Inquéritos e QuestionáriosRESUMO
Background: Adipolin, the novel adipokine that is proposed to be reduced in diabetes, obesity and inflammation, may improve glycemic control. It is known that coenzyme Q10 could improve insulin sensitivity. The aim of the current study was to investigate the effect of Q10 supplementation on adipolin concentration and glucose metabolism in overweight and obese diabetic patients. Material & Methods: Sixty four patients with type 2 diabetes and 25Assuntos
Adipocinas/sangue
, Diabetes Mellitus Tipo 2
, Obesidade
, Ubiquinona/análogos & derivados
, Adulto
, Diabetes Mellitus Tipo 2/sangue
, Diabetes Mellitus Tipo 2/tratamento farmacológico
, Feminino
, Hemoglobinas Glicadas/metabolismo
, Humanos
, Masculino
, Pessoa de Meia-Idade
, Obesidade/sangue
, Obesidade/dietoterapia
, Ubiquinona/administração & dosagem
RESUMO
OBJECTIVE: To investigate whether iron supplementation can improve thyroid hormone function in iron-deficient adolescent girls. DESIGN: A double-blind randomized intervention study. SETTING: The study was performed from 2002 through 2003 in the Islamic Republic of Iran. SUBJECTS: 103 iron-deficient non-anaemic girls who fulfilled all inclusion criteria were included, and 94 subjects successfully completed the study. INTERVENTIONS: Patients were randomly assigned to one of four groups and treated with a single oral dose of 190 mg iodine plus 300 mg ferrous sulphate 5 times/week (n=24), 300 mg ferrous sulphate 5 times/week (n=23), a single oral dose of 190 mg iodine (n=25), or a placebo (n=22) for 12 weeks. RESULTS: All groups were comparable at baseline. After the intervention, there was a significant increase in ferritin and transferrin saturation in the iron+iodine group (17.6 vs 8.7 microg/dl, and 18.8 vs 7.2%, respectively, P<0.001 for both) and in the iron group (P<0.001 for both). Urinary iodine doubled in the iron+iodine group and in the iodine group (P<0.001 for both). Thyroid indices tT4, tT3 and T3RU increased and reverse RT3 decreased in the iron+iodine group (10 vs 8.9 microg/dl, P< 0.001; 143 vs 138 microg/dl, P<0.05; 32.3 vs 28.4%, P<0.001 and 24.8 vs 44.2 ng/dl, P<0.001, respectively) and in the iron group. These two groups did not differ for any of the four indices, but both differed significantly from the iodine and placebo groups. CONCLUSIONS: Our results indicate that improvement of iron status was accompanied by an improvement in some indices of thyroid hormones. SPONSORSHIP: This study was supported by the Dean of Research Affairs of the Tehran University of Medical Sciences.
Assuntos
Iodo/uso terapêutico , Deficiências de Ferro , Ferro da Dieta/uso terapêutico , Hormônios Tireóideos/sangue , Oligoelementos/uso terapêutico , Adolescente , Suplementos Nutricionais , Método Duplo-Cego , Quimioterapia Combinada , Feminino , Ferritinas/sangue , Hemoglobinas/análise , Humanos , Iodo/urina , Irã (Geográfico) , Ferro/sangue , Ferro da Dieta/metabolismo , Placebos , Oligoelementos/metabolismo , Transferrina/análiseRESUMO
OBJECTIVE: Furazolidone, an old but cheap antibiotic, was shown to be a good alternative to metronidazole in triple therapy for Helicobacter pylori eradication in areas where metronidazole resistant bacteria are common, but randomized studies are lacking. AIM: A randomized controlled trial to determine the efficacy and safety of furazolidone compared to metronidazole in classic quadruple therapy for eradication of H. pylori infection in duodenal ulcer patients. METHODS: Patients with endoscopically proven duodenal ulcer and positive urease test were randomized to receive ranitidine 300 mg, amoxycillin 1000 mg and bismuth subcitrate 240 mg b.d, with either furazolidone 200 mg b.d (RABF), or metronidazole 500 mg b.d. (RABM) for 2 weeks. Compliance and side-effects were monitored and recorded by table diary. H. pylori eradication was assessed at least 4 weeks after the completion of therapy with 14C-urea breath test. RESULTS: A total of 106 patients were enrolled and 101 (59 male, 42 female, mean age=40 +/- 11 years) completed the study. Endoscopic findings and demographic data were comparable in both groups. Intention-to-treat eradication rates were 75% and 55% (P=0.03) and per protocol eradication rates were 82 and 56% (P=0. 006) in the RABF and RABM groups, respectively. Side-effects were reported by 13 patients (27%) in the RABF group (one stopped treatment) compared to five patients (10%) in the RABM group (P=0. 04). CONCLUSION: Quadruple therapy containing furazolidone, instead of metronidazole, results in a significantly higher H. pylori eradication rate in Iranian duodenal ulcer patients.
Assuntos
Antibacterianos/uso terapêutico , Úlcera Duodenal/tratamento farmacológico , Úlcera Duodenal/microbiologia , Furazolidona/uso terapêutico , Infecções por Helicobacter/tratamento farmacológico , Helicobacter pylori , Metronidazol/uso terapêutico , Adulto , Antibacterianos/efeitos adversos , Combinação de Medicamentos , Feminino , Furazolidona/efeitos adversos , Infecções por Helicobacter/microbiologia , Humanos , Masculino , Metronidazol/efeitos adversos , Estudos Prospectivos , Urease/análiseRESUMO
This study concerns the effects of several pre-transplant features on outcome for patients with beta thalassemia major who underwent bone marrow transplantation (BMT). Seventy patients with beta thalassemia major underwent bone marrow transplantation during the period 1991-1997 in Shariati Hospital in Tehran, Iran. The survival and rejection curves levelled off at 8 and 18 months after transplantation at 82.6% and 11.4%, respectively. Pre-transplant clinical features (age, serum ferritin, portal fibrosis, hepatomegaly and quality of chelation therapy) were examined for their effects on survival and recurrence of thalassemia in this group of patients who were less than 16 years old. Increasing age, presence of portal fibrosis and increasing serum ferritin were significantly associated with reduced probability of survival (P = 0.0047, P = 0.016 and P = 0.024, respectively). Hepatomegaly and inadequate pre-transplant chelation therapy which were documented as poor prognostic factors in previous studies, were not evaluable in this study. We also showed the benefits of transplanting more than 5.5 x 10(8)/kg cells in this group of patients with no increase in complications.
Assuntos
Transplante de Medula Óssea , Talassemia beta/terapia , Adolescente , Fatores Etários , Criança , Pré-Escolar , Feminino , Ferritinas/sangue , Rejeição de Enxerto , Sobrevivência de Enxerto , Humanos , Irã (Geográfico) , Masculino , Prognóstico , Fatores de RiscoRESUMO
OBJECTIVE: Investigation of the relationship between educational level, body mass index (BMI), waist-to-hip ratio (WHR), physical activity and parity in a group of Iranian men and women living in Tehran. DESIGN: A cross-sectional study in a group of Iranian men and women. The subjects were classified into two educational levels: low education (< or =12 y schooling) and high education (>12 y schooling); and BMI, WHR, physical activity and parity (in women) were compared in two groups in men and women, separately. SETTING: Metabolic Unit of Tehran University of Medical Sciences in Tehran. SUBJECTS: Three hundred and fifteen men aged 33.1 (22-46) and 403 women aged 27.9 (22-45). RESULTS: After controlling for age and smoking, women with a higher level of education showed a significantly lower mean BMI than less educated women (24.8+/-4.2 vs 28.3+/-4.9, P <0.01), while more educated men had a higher mean BMI than less educated men (28.4+/-4.3 vs 26.7+/-4.5). In multiple regression analysis, physical activity in leisure time in men and years of education in women were the only determinants of BMI. After controlling for BMI, WHR was not related to the level of education in either men or women. CONCLUSION: The present data indicated an educational difference in BMI for the study population. In Iranian women, like the women in developed countries, the level of education was negatively related to BMI, while in men the association was positive. SPONSORSHIP: This work was financially supported by the Institute of Public Health, Tehran University of Medical Sciences.
Assuntos
Índice de Massa Corporal , Escolaridade , Estilo de Vida , Adulto , Constituição Corporal , Estudos Transversais , Feminino , Humanos , Irã (Geográfico) , Atividades de Lazer , Masculino , Pessoa de Meia-Idade , Paridade , Aptidão Física , Análise de Regressão , FumarRESUMO
OBJECTIVE: To study the efficacy of levothyroxine suppressive therapy in the management of benign thyroid nodules. METHODS: We performed a double-blind clinical trial comparing levothyroxine treatment (1.5 to 2.0 mg/kg of body weight daily) (N = 32) with placebo (N = 30) for a 1-year period in patients with a benign, cold thyroid nodule confirmed by biopsy and 99mTc-pertechnetate scanning, who were randomly assigned to the treatment or control group. High-resolution sonography was used to measure the size of the nodules before and after the treatment. Suppression of thyrotropin was evaluated by the administration of thyrotropin-releasing hormone to 10 patients randomly in each group. RESULTS: The mean volume of the thyroid nodules decreased significantly after 6 months in both the levothyroxine group (from 12.8 +/- 11.9 mL to 9.4 +/- 9.8 mL; P = 0.003) and the placebo group (from 13.2 +/- 10.2 mL to 11.5 +/- 8.0 mL; P = 0.003). After 12 months, however, the volume of the nodules had increased. Thus, no significant decrease was found in the mean nodule volume in either study group at 1 year in comparison with the mean volume at baseline (final mean volume: 12.4 +/- 16.7 mL in the levothyroxine group and 11.7 +/- 13.6 mL in the placebo group). CONCLUSION: Suppressive therapy with levothyroxine for a period of 12 months proved to be ineffective in significantly reducing the size of the thyroid nodules in our patients despite effective suppression of the thyrotropin level.
RESUMO
BACKGROUND/OBJECTIVES: Oxidative stress (OS) is thought to be involved in both development of type 1 diabetes (T1D) and its further complications. In this study, certain biomarkers of OS were compared among the subjects with T1D, their non-diabetic siblings and unrelated healthy controls. SUBJECTS/METHODS: Known cases of T1D from both sexes aged 5-25 years were enrolled in a case-control study (n(1)=60). There were two control groups; non-diabetic siblings (n(2)=60) and unrelated apparently healthy subjects (n(3)=60). Anthropometric, dietary and laboratory assessments were done. RESULTS: There was no significant difference in dietary data among the groups. Total antioxidant capacity was significantly lower in T1D than both related and unrelated controls (1.6 ± 0.05, 1.7 ± 0.05 and 1.8 ± 0.06 mmol BSA equivalent/l, respectively, P=0.044). Both T1D subjects and their siblings showed lower glutathione peroxidase (GSH-px) levels (median (interquartile range): 22.2 (28.6), 29.9 (23) and 41.8 (73.6) U/ml, respectively, P=0.006). On the contrary, superoxide dismutase concentrations were significantly higher in T1D group and the siblings than unrelated healthy controls (243 (45.3), 157.8 (176.9) and 27.9 (8.7) U/l, respectively, P<0.001). Serum concentrations of GSH correlated with energy intake in the siblings (r=0.521, P<0.001) and unrelated controls (r=0.268, P=0.042) but not in T1D group. The associations remained significant after controlling for blood glucose (r=0.437, P=0.001 and r=0.420, P=0.011, respectively) in both the groups. CONCLUSION: Augmented OS in the siblings may indicate an increased requirement for antioxidants in genetically diabetes-prone subjects.
Assuntos
Diabetes Mellitus Tipo 1/metabolismo , Estresse Oxidativo/fisiologia , Adolescente , Adulto , Glicemia/metabolismo , Estudos de Casos e Controles , Criança , Pré-Escolar , Diabetes Mellitus Tipo 1/sangue , Diabetes Mellitus Tipo 1/enzimologia , Feminino , Glutationa/sangue , Glutationa Peroxidase/sangue , Humanos , Irã (Geográfico) , Masculino , Irmãos , Estatísticas não Paramétricas , Superóxido Dismutase/sangue , Adulto JovemRESUMO
Long lasting insecticide treated nets (LLINs) have been advocated as an effective tool for prevention and control of malaria. Olyset net was the first LLINs which became commercially available and obtained WHO approval. According to the national strategic plan on evaluation of Olyset net, a field trial was conducted to determine the efficacy of these nets against malaria vectors in an endemic area in the southeast of Iran. Fourteen villages with similar topographical and epidemiological situations were selected and randomly assigned to two clusters of the study: Olyset net and untreated net. Distribution of nets was carried out to cover 100% of the population in Olyset net and untreated net cluster. Anopheline mosquitoes were collected monthly using different WHO standard methods in both areas to determine their abundance, feeding pattern and resting behaviour. Human blood index was determined using ELISA test. Additionally, Olyset nets were evaluated for their biological activity using WHO cone bioassay test by susceptible colony of Anopheles stephensi (Beech strain) and then for insecticide residues by employing high performance thin layer chromatography. Malaria incidence was measured by passive and active case detection from all study population. In total 2115 adult anopheline mosquitoes were collected and identified using morphological characters. They comprised of seven species: Anopheles dthali (Liston), A. culicifacies (Giles), A. stephensi (Liston), A. superpictus (Grassi), A.fluviatilis (James), A. moghulensis (Christophers) and A. turkhudi (Liston). A. dthali, A. culicifacies and A. stephensi were most prevalent species in both areas. In the Olyset net study area, there was a significant reduction of 41.1%, 54.4%, 59.39% and 64.1% in the indoor-resting density of A. culicifacies, A. stephensi, A. dthali and A. superpictus, respectively, with an overall reduction of 39.3% in total mosquitoes in comparison with untreated net area. A significant reduction was also observed in human blood index of vector species in the Olyset net villages. Bioefficacy test results of Olyset nets showed that the median knockdown time was 1.48 and 3.25min, while the average mortality rate was 100% and 72.3%±7.07 in baseline and after 1 year of intervention, respectively. The average permethrin content reached to 68.31% (683.1mg/m(2)) of the initial insecticide dose of 937±21.69mg/m(2) (nearly 1000mg/m(2)) at the end of intervention. Malaria incidence was reduced by 96.6% and 64.8% in the village with Olyset nets and in the villages with untreated nets, respectively. During intervention period, there was a reduction of 93.2% in malaria incidence in Olyset net area as compared to the untreated area. This study indicated that Olyset nets have a major impact on malaria vectors and disease burden; therefore it could be recommended as an effective personal protection tool for malaria control in malarious areas.
Assuntos
Doenças Endêmicas , Mosquiteiros Tratados com Inseticida , Inseticidas/farmacologia , Malária/epidemiologia , Malária/prevenção & controle , Controle de Mosquitos/métodos , Permetrina/farmacologia , Animais , Anopheles/classificação , Anopheles/efeitos dos fármacos , Humanos , Incidência , Irã (Geográfico)/epidemiologiaRESUMO
Multiple sclerosis (MS) can influence all aspects of a patient's health. This study determines the main factors affecting quality of life (QoL) in Iranian MS patients. QoL (Multiple Sclerosis Impact Scale; MSIS-29), disability (Expanded Disability Status Scale; EDSS) and depression (Beck Depression Inventory; BDI) were assessed in 106 MS patients. EDSS, clinical course and MS duration significantly correlated with physical MSIS-29. Depression highly correlated with both physical and psychological MSIS-29. Regression analyses showed that depression and EDSS predicted physical health. Psychological health was predicted by depression. These findings highlight that depression and physical disability strongly influence QoL in Iranian MS patients.
Assuntos
Transtorno Depressivo/diagnóstico , Pessoas com Deficiência/psicologia , Esclerose Múltipla/psicologia , Qualidade de Vida , Adolescente , Adulto , Estudos Transversais , Transtorno Depressivo/etiologia , Avaliação da Deficiência , Feminino , Humanos , Irã (Geográfico) , Masculino , Pessoa de Meia-IdadeRESUMO
OBJECTIVE: Overweight has become a public health problem in most developing countries. Evidence suggests that adolescence is a critical period in determining adulthood obesity and its complications. The present study was carried out to assess the prevalence of overweight and obesity among secondary school students. DESIGN AND SETTING: This descriptive study was conducted in Tehran city, 2000-2001. Body weight and height were measured and body mass index (BMI) values were calculated. Underweight, overweight and obesity were defined as <5th, > or = 85th and > or = 95th percentile, respectively, of age- and sex-specific BMI values from the National Center for Health Statistics/Centers for Disease Control and Prevention (2000). SUBJECTS: Using a multistage sampling method, 2321 students (1068 males and 1253 females) aged 11-16 years were assessed in Tehran, the capital city of Iran. RESULTS: The overall prevalences of overweight and obesity were 21.1 and 7.8%, respectively. The prevalence of overweight among girl students (i.e. 23.1%; 95% confidence interval (CI) 20.8-25.4) was significantly higher than that among boys (i.e. 18.8%; 95% CI 16.5-21.1, P=0.01) even after adjustment for age (odds ratio 1.26, 95% CI 1.03-1.55, P=0.02). No significant risk of obesity associated with age was found in girls or boys. In both sexes, median values of age-specific BMI in this study were statistically higher than corresponding values collected in Tehrani adolescents 10 years ago (P=0.03). Similarly, a significant difference was seen between girl students in this study and the reference population (P=0.03). CONCLUSION: According to this study, overweight, especially in girls, should be considered an epidemic health problem among adolescent students in Tehran.