Bioactive chitosan/poly(ethyleneoxide)/CuFe2O4 nanofibers for potential wound healing.

Wound healing is a complex process that often requires intervention to accelerate tissue regeneration and prevent complications. The goal of this research was to assess the potential of bioactive chitosan@poly (ethylene oxide)@CuFe2O4 (CS@PEO@CF) nanofibers for wound healing applications by evaluating their morphology, mechanical properties, and magnetic behavior. Additionally, in vitro and in vivo studies were conducted to investigate their effectiveness in promoting wound healing treatment. The nanoparticles exhibited remarkable antibacterial and antioxidant properties. In the nanofibrous mats, the optimal concentration of CuFe2O4 was determined to be 0.1% Wt/V. Importantly, this concentration did not adversely affect the viability of fibroblast cells, which also identified the ideal concentration. The scaffold's hemocompatibility revealed nonhemolytic properties. Additionally, a wound-healing experiment demonstrated significant migration and growth of fibroblast cells at the edge of the wound. These nanofibrous mats are applied to treat rats with full-thickness excisional wounds. Histopathological analysis of these wounds showed enhanced wound healing ability, as well as regeneration of sebaceous glands and hair follicles within the skin. Overall, the developed wound dressing comprises CuFe2O4 nanoparticles incorporated into CS/PEO nanofibrous mats demonstrating its potential for successful application in wound treatment.

Cell-loaded genipin cross-linked collagen/gelatin skin substitute adorned with zinc-doped bioactive glass-ceramic for cutaneous wound regeneration.

An optimal tissue-engineered dermal substitute should possess biocompatibility and cell adhesion conduction to facilitate fibroblast and keratinocyte infiltration and proliferation, as well as angiogenesis potential to escalate wound healing. Zinc was doped to bioactive glass-ceramic (Zn-BGC) to promote biocompatibility and angiogenesis properties. Zn-BGC was then incorporated into a collagen (Col) and gelatin (Gel) porous scaffold. The bioactive porous bionanocomposite exhibited biocompatibility along with improved cell attachment and proliferation. Scaffolds including Col-Gel/Zn-BGC with or without mouse embryonic fibroblasts were applied on full-thickness skin wounds on the BALB/c mice to assess their wound healing potential in vivo. The results indicated that the biodegradation rate of the Col-Gel/Zn-BGC nanocomposites was comparable to the rate of skin tissue regeneration in vivo. Macroscopic wound healing results showed that Col-Gel/Zn-BGC loaded with mouse embryonic fibroblast possesses the smallest wound size, indicating the fastest healing process. Histopathological evaluations displayed that the optimal wound regeneration was observed in Col-Gel/Zn-BGC nanocomposites loaded with mouse embryonic fibroblasts indicated by epithelialization and angiogenesis; besides the number of fibroblasts and hair follicles was increased. The bioactive nanocomposite scaffold of Col-Gel containing Zn-BGC nanoparticles loaded with mouse embryonic fibroblasts can be employed as a desirable skin substitute to ameliorate cutaneous wound regeneration.