Immunotoxic responses of chronic exposure to cypermethrin in common carp.

In the current study, laboratory evaluations were made to assess the immunomodulatory effect of cypermethrin on fingerlings of common carp (Cyprinus carpio L.). Results showed that 96-h LC50 of cypermethrin in common carp was estimated at 0.85 µg/L. Fish were exposed for 21 days to cypermethrin at three sub-lethal concentrations of 0.042, 0.085, and 0.17 µg/L that represented 5, 10, and 20%, respectively, of the 96-h LC50 of the pesticide for this fish species. Blood samples were taken after 7, 14, and 21 days of exposure. Immunological indices and resistance against bacterial infection were determined. Compared to the control group, the fish exposed to cypermethrin showed a significant increase in neutrophil ratio but exhibited a significant decrease in leukocyte number and lymphocyte ratio in treatments exposed to 0.17 and/or 0.085 µg/L after 21 days of exposure (p < 0.05). Serum protein level was significantly decreased in group exposed to 0.17 µg/L on day 14 and also in groups exposed to 0.085 and 0.17 µg/L on day 21 (p < 0.05). Immunoglobulin value was significantly reduced in groups exposed to 0.085 and 0.17 µg/L after 21 days of exposure (p < 0.05). Serum lysozyme activity and phagocytic activity were significantly decreased following exposure to 0.17 µg/L determined on days 14 and 21, post-exposure (p < 0.05). Mortality rate following the challenge with Aeromonas hydrophila significantly increased in fish exposed to 0.17 µg/L of cypermethrin. Overall, the present results indicate severe immunotoxicological effects of cypermethrin in common carp. Therefore, the use of cypermethrin in the proximities of common carp farms should be carefully considered.

Effects of microplastic exposure on the blood biochemical parameters in the pond turtle (Emys orbicularis).

The accumulation of microplastics (MPs) is a growing problem in aquatic ecosystems. Despite increased research on MPs in the last decade, their potential threat to freshwater ecosystems remains an open question. In the present study, the negative impacts of MPs were investigated on blood biochemical parameters in the European pond turtle (Emys orbicularis). Pond turtles were distributed into three experimental groups (n = 9 for each group) and were fed diets containing 250, 500, and 1000 mg MPs (PE100 polyethylene) per kg of food for 30 days, and a control group fed with a standard uncontaminated diet. The results indicated that exposure to 500 and 1000 mg kg-1 MPs caused a significant increase in the activities of alanine and aspartate aminotransferases, and in the levels of cholesterol, glucose, creatinine, urea, and calcium (Ca+2) compared with the control group. On the contrary, the activity of gamma-glutamyl transferase and the levels of total protein, albumin, total immunoglobulins, and phosphorus were significantly reduced in E. orbicularis exposed to 500 and 1000 mg kg-1 MPs when compared with the controls. In all the MP-exposed groups, the activity of lactate dehydrogenase and globulin and magnesium (Mg+2) levels were significantly reduced; while creatine phosphokinase and alkaline phosphatase activities were increased with respect to the control turtles. A significant decrease in triglyceride levels was reported in E. orbicularis exposed to 1000 mg kg-1 MPs. MPs intake induced notable alterations in blood biochemical parameters of E. orbicularis. These results suggest that changes in the blood biochemical parameters could be an appropriate bio-indicator to evidence the existence of tissue damage in E. orbicularis.

Acute exposure to chlorpyrifos and glyphosate induces changes in hemolymph biochemical parameters in the crayfish, Astacus leptodactylus (Eschscholtz, 1823).

Acute and subacute toxicity tests for chlorpyrifos and glyphosate were performed on the crayfish, Astacus leptodactylus. The crayfish were divided into a control group and four experimental groups with exposure from 50.18 to 301.11 µg L-1 of chlorpyrifos for 96 h under semi-static test conditions. In the same experiment, the crayfish were exposed to different concentrations of glyphosate (from 0.0, 8.14 to 13.05 mg L-1) for 96 h. Mortality was recorded, and the median lethal concentrations (LC50) were calculated using probit analysis. The 96 h LC50 values of chlorpyrifos and glyphosate to A. leptodactylus were 49.55 ±â€¯4.66 µg L-1 and 7.83 ±â€¯0.50 mg L-1, respectively. The results showed that chlorpyrifos was a few hundred times more toxic than glyphosate for the crayfish. Then, the crayfish were exposed to different subacute concentrations of chlorpyrifos (12.5 and 25 µg L-1) and glyphosate (2 mg L-1 and 4 mg L-1) for seven days, and their toxicity was evaluated through several hemolymph biochemical parameters. Chlorpyrifos and glyphosate exposure caused a significant (P < 0.01) increase in the activity of lactate dehydrogenase (LDH), and gamma-glutamyl transferase (GGT). The results showed a significant (P < 0.01) decrease in acetylcholinesterase (AChE), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and phenoloxidase (PO) activities in the hemolymph of the crayfish exposed to chlorpyrifos and glyphosate. The total protein and cholesterol levels showed a significant decrease (P < 0.01) in the hemolymph of the crayfish after seven days of exposure to chlorpyrifos and glyphosate. There was a significant increase in triglycerides and glucose levels in treated crayfish. These results show that chlorpyrifos is highly toxic, while glyphosate is moderately toxic to A. leptodactylus. According to the results, subacute concentrations of chlorpyrifos and glyphosate could lead to a collapse of cellular homeostasis and changes in the hemolymph biochemical parameters in the crayfish. Physiological changes caused by exposure to chlorpyrifos and glyphosate in A. leptodactylus may have direct effects on the survival of these organisms.