Effectiveness of three antimicrobial mouthrinses on the disinfection of toothbrushes stored in closed containers: a randomized clinical investigation by DNA Checkerboard and Culture.

OBJECTIVES: The aim of this in vivo study was to evaluate the efficacy of three antimicrobial solutions on the disinfection of toothbrushes after storage in closed containers. MATERIALS AND METHODS: Sixteen healthy subjects were enrolled in this randomized cross-over clinical investigation. The study was conducted in four phases, in which mouthrinses (chlorhexidine gluconate-based or cetilpiridinium-based) and sterile tap water (control group) were used to individually store used toothbrushes in closed containers during 7 days of toothbrushing. Five toothbrushes were used as negative control for bacterial colonisation before contact with oral cavity. Conventional culture and DNA Checkerboard hybridization were used to detect bacterial contamination on the toothbrushes. Subsequently, the number of bacterial species on the bristles was estimated by the DNA Checkerboard method. RESULTS: One toothbrush presented bacterial contamination in the negative control test. Both culture and DNA Checkerboard showed positive signals of bacterial contamination in the toothbrushes with no differences in the frequency of detection. The control group showed higher total bacterial counts when compared with the mouthrinse groups. Porphyromonas gingivalis had the highest bacterial count followed by Parvimonas micra. CONCLUSION: Culture and DNA Checkerboard showed positive signals of bacterial contamination. Mouthrinses that contains 0.12% of chlorhexidine gluconate were more effective in reducing bacterial colonisation on the toothbrushes.

Evaluation of the efficacy of chemical disinfectants for disinfection of heat-polymerised acrylic resin.

OBJECTIVE: This study evaluated the efficacy of disinfectants on the internal aspect of heat-polymerised acrylic resin contaminated with microbial strains. BACKGROUND: Dentures absorb oral fluids and become contaminated by different microorganisms. METHODS: Two hundred and fifty rectangular specimens were made of heat-polymerised acrylic resin, and then divided into five groups corresponding to the microbial strains (Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, S. mutans and Enterococcus faecalis). After contamination, the specimens were immersed in 1 and 2% sodium hypochlorite and 2% glutaraldehyde for periods of 5, 10 and 15 min. The specimens were placed into tubes containing different broths and incubated at 35°C and then visually analysed. Turbidity in the medium indicated microbial growth. The Fisher's exact test was used in the analysis of the results. RESULTS: The strain E. faecalis was the most resistant to the disinfectant solutions, and among them, glutaraldehyde was more effective than 2 and 1% hypochlorite for disinfection for 5 min; in the 10-min period there were no differences between the disinfectants. In 15 min of immersion, 1% hypochlorite and glutaraldehyde were more effective than 2% hypochlorite. CONCLUSIONS: Disinfection for 10 min with 1% hypochlorite and glutaraldehyde is effective in disinfecting the internal aspect of heat-polymerised acrylic resin.

Use of resin-bonded extracoronal attachments with removable partial denture: case report.

INTRODUCTION: Resin-bonded extracoronal attachments may be indicated for the abutment teeth of removable partial dentures, especially for anterior teeth when a cingulum rest must be provided. This type of treatment has a series of advantages such as minimal tooth reduction, supragingival margins, favourable stress distribution, and improved aesthetic appearance. OBJECTIVE: To report a clinical case of oral rehabilitation using a combination of resin-bonded extracoronal attachments joined by a Dolder bar with a removable partial denture. CASE REPORT: A 60-year-old male patient with only the canines in the maxillary arch was restored with a combination of resin-bonded extracoronal attachments joined by a Dolder bar and a removable partial denture. CONCLUSION: Dentures with resin-bonded extracoronal attachments can have a number of advantages over traditional clasp-retained removable partial dentures.

An easy technique to obtain articulated casts using the palate for the interocclusal record.

This article describes a highly efficient technique to obtain articulated casts. Interocclusal recording is carried out using the palate as an area of registration without having to remove or section provisional prostheses while also maintaining the established vertical dimension.

Pré-molarização: uma alternativa para reabilitações implantossuportadas em região posterior / Premolarization: an alternative to implant-supported rehabilitations in the posterior region

Diferenças anatômicas e biomecânicas fazem do tratamento com implantes em regiões posteriores substancialmente diferentes daquele em região anterior. Sem os implantes e diante da perda de dentes posteriores, as opções de tratamento incluem próteses parciais fixas extensas ou próteses parciais removíveis, especialmente quando não há um pilar distal disponível. Contemporaneamente, as próteses implantossuportadas representam um tratamento altamente eficaz e aceitável para a reabilitação oral de indivíduos parcialmente edêntulos, entretanto, tornou-se necessário reavaliar e modificar alguns conceitos previamente estabelecidos devido aos impactos negativos proporcionados por modelos de próteses nos quadrantes posteriores, como o desfavorável perfil de emergência, dificultando o controle de placa bacteriana, a impacção alimentar e a dissipação não axial das forças oclusais. Modificando-se o planejamento dessas próteses, onde coroas de molares sejam substituídas por coroas de pré-molares, proporciona-se uma possível solução para esses problemas. Assim, três pré-molares podem equivalentemente substituir dois molares, situação esta que caracteriza o conceito de pré-molarização. O objetivo do presente estudo foi relacionar os aspectos funcionais e biomecânicos envolvidos nas próteses implantossuportadas em regiões posteriores, estabelecendo-se assim as possíveis vantagens da aplicação da pré-molarização nas reabilitações orais sobreimplantes.

mplant treatment in posterior regions is substantially different, both anatomical and biomechanically, from the anterior regions. Without considering dental implants, and with the loss of posterior teeth, treatment options include an extensive fixed or removable partial denture, especially when no distal tooth abutments are available. Nowadays, the implant-supported dental prosthesis represent a highly effective and acceptable treatment for oral rehabilitation of partially edentulous individuals, however, it became necessary to reevaluate and modify some of the concepts previously established due to the negative impacts provided by prosthesis models in the posterior regions, as the unfavorable emergence profile, making it difficult to control plaque, food impaction and non-axial dissipation of the occlusal forces. By modifying prosthesis design, where molar crowns are replaced by premolars crowns, a possible solution is offered. Thus, three premolars can equivalently replace two molars, a situation that characterizes the concept of premolarization. The aim of this study was to compare the biomechanical and functional aspects involved in implant-supported dental prosthesis for posterior regions, thus establishing the potential benefits of applying the premolarization concept to the implant-supported oral rehabilitation.