Intelligent Drone Positioning via BIC Optimization for Maximizing LPWAN Coverage and Capacity in Suburban Amazon Environments.

This paper aims to provide a metaheuristic approach to drone array optimization applied to coverage area maximization of wireless communication systems, with unmanned aerial vehicle (UAV) base stations, in the context of suburban, lightly to densely wooded environments present in cities of the Amazon region. For this purpose, a low-power wireless area network (LPWAN) was analyzed and applied. LPWAN are systems designed to work with low data rates but keep, or even enhance, the extensive area coverage provided by high-powered networks. The type of LPWAN chosen is LoRa, which operates at an unlicensed spectrum of 915 MHz and requires users to connect to gateways in order to relay information to a central server; in this case, each drone in the array has a LoRa module installed to serve as a non-fixated gateway. In order to classify and optimize the best positioning for the UAVs in the array, three concomitant bioinspired computing (BIC) methods were chosen: cuckoo search (CS), flower pollination algorithm (FPA), and genetic algorithm (GA). Positioning optimization results are then simulated and presented via MATLAB for a high-range IoT-LoRa network. An empirically adjusted propagation model with measurements carried out on a university campus was developed to obtain a propagation model in forested environments for LoRa spreading factors (SF) of 8, 9, 10, and 11. Finally, a comparison was drawn between drone positioning simulation results for a theoretical propagation model for UAVs and the model found by the measurements.

Microsatellite variation revealed panmictic pattern for Triatoma brasiliensis (Triatominae: Reduviidae) in rural northeastern Brazil: the control measures implications.

BACKGROUND: Triatoma brasiliensis Neiva, 1911 is the main vector of Trypanosoma cruzi in the caatinga of Northeastern Brazil. Despite of its epidemiological relevance, there are few studies on its genetic variability. Using microsatellite markers, we characterized the variability and dynamics of infestation and reinfestation of T. brasiliensis after residual insecticide spraying in five surveys conducted in a well-defined rural area located in the municipality of Tauá, Ceará, between 2009 and 2015. We evaluated: (1) general variability among local of captures; (2) variability along the time analysis (2009, 2010 and 2015); (3) and reinfestation process. RESULTS: On the analysis (1) global and pairwise FST values suggested absence of clusters among the area. AMOVA indicated that total variation is mainly represented by individual differences. Absence of clustering indicates a panmitic unit, with free gene flow. For (2), Pairwise FST indicated alterations in the genetic profile of the triatomines along the time. (3) Analysis of the reinfestation process showed that the domiciliary units investigated had different sources of infestation despite of its proximity. CONCLUSIONS: Observed homogeneity can be explained by the great dispersal capacity of T. brasiliensis, overlapping the different environments. Persistent house infestation in Tauá may be attributed to the occurrence of postspraying residual foci and the invasion of triatomines from their natural habitats.

Oblique astigmatism and field curvature measured with light-field imaging.

The testing of astigmatism and field curvature of an optical system with light-field imaging is proposed. The method consists in measuring the depth map, obtained with a plenoptic camera, of the image of a test pattern formed by the optical system. To demonstrate the accuracy of the method, the virtual image formed by a plano-convex lens was tested. The resulting depth map was compared with the sagittal and tangential foci distances calculated with the Coddington theory. The proposed method could be applied to the quality testing of ocular optics, including eyeglasses, eyepieces, virtual reality goggles, and augmented reality devices.

Ions release Evaluation and Changes in Mini-implant Orthodontic Surface.

AIM: To evaluate, in vitro, the mini-implant surface changes and the release of ions after immersion in artificial saliva during follow-up of 60 and 120 days. MATERIALS AND METHODS: As for the surface features, examined in a scanning electron microscope (SEM), before and after immersion in artificial saliva, there was a rough and uneven surface, suggestive of corrosion areas for the two trademarks evaluated after 120 days of immersion. The extracts generated in artificial saliva analysis were submitted to energy dispersive spectroscopy to identify the solid corrosion products produced on the surfaces of miniscrews. RESULTS: Both SIN miniscrews and Neodent brands were observed to release minimal quantities of silver ions, chromium, iron, nickel, titanium, and vanadium. Regarding titanium, this index varied from 88.84% in the control group of Neodent brand, and 91.29% in the control group of SIN brand. For the aluminum content, the quantities ranged from 4.91% in group immersed for 60 days in Neodent brand to 8.71% for the SIN control group. Considering vanadium, the index ranged from 2.65% in the group immersed for 120 days to 4.53% in the control group, both for Neodent brand. Statistically significant differences in iron ion were observed between the control group and the miniscrews brand SIN after 60 and 120 days and for Neodent after 60 days of immersion. The titanium ions suffered statistically significant decrease for both brands after 120 days of storage when compared with the control group. CONCLUSION: The studied miniscrews showed results consistent with the biosafety of alloys for use, in vivo. CLINICAL SIGNIFICANCE: The knowledge of the physical/chemical state of corrosion products released in the oral cavity is very important for the toxicological assessment of metal alloys used in dental miniscrews.

Evaluation of cervico-occlusal dimensions of maxillary and mandibular incisor brackets for lingual orthodontics.

AIM: The purpose of this study was to analyze the cervico-occlusal dimensions of brackets used in lingual orthodontics for maxillary and mandibular central and lateral incisors, to compare them with the numbers informed by the manufacturer. MATERIALS AND METHODS: The brackets were placed in a template and analyzed by scanning electron microscopy (SEM), evaluating the images with the aid of a software that allowed determination of reference points, tracing of lines on the bracket images and measurement of the distances. RESULTS: Brackets manufactured by Dm Ceosa Hiro, Tecnident PSWb and Dentaurum Magic did not present statistically significant differences between the mean values of slot heights and the values informed by manufacturers. Conversely, the brands Ormco 7th generation, Ormco STb, GAC Innovation L, ORJ-ORG, Tecnident, Ormco STb LSW and Adenta evolution STL exhibited statistically significant differences. CONCLUSION: In general, there was a deficiency in the standar-dization of cervico-occlusal measurements of slots of the brackets analyzed. CLINICAL SIGNIFICANCE: Manufacturers should be attentive to the quality control of their materials. Future studies will analyze the actual clinical influence of these findings on the orthodontic mechanics.

Upper incisors' positions after extraction.

The aim of this research was to verify the amount of horizontal and vertical movement and incisor inclination of upper incisors and correlate these with Edgewise and Alexander brackets use and the presence of overbite during anterior retraction in sliding mechanics. The sample was composed of 40 adult patients divided into 2 groups, treated with Edgewise and Alexander brackets (20 each) subdivided in 2 groups (10 each), according to the presence or absence of deep bite. Treatment consisted of 4 extraction cases with sliding mechanics with the 2 different brackets. Pre- and post-treatment cephalograms were measured and the values of interest submitted to descriptive statistical analysis, ANOVA at 5%, the Tukey test and Pearson's correlation. Upper incisor retraction was not related to the brackets used nor to the presence of deep bite, though lingual tipping was greater when Edgewise brackets were used and deep bite was absent. No statistically significant differences in upper incisor vertical movements were observed and no correlation was determined between upper incisor intrusion and lower incisor labial tipping in overbite correction or in upper incisor retraction and lower incisor labial tipping for overjet correction. Bracket prescription and its interaction with deep bite were significant and Edgewise brackets without deep bite showed the worst inclination control. It was concluded that bracket prescriptions are important to increase control of sliding mechanics.

Biomechanical analysis of total arch maxillary distalization using infrazygomatic crest miniscrews: a finite element analysis study.

AIM: To evaluate the maxillary incisors and canine's immediate movement tendency using three different power arms (PA) height levels during total arch maxillary distalization supported on infrazygomatic crest (IZC) miniscrews according to finite element analysis (FEA). METHODS: Three finite element models of the maxilla were developed based on CBCT imaging of a teenage male patient presenting a Class II Division 1 malocclusion in the early permanent dentition. Maxillary complex, periodontium, orthodontic accessories, IZC miniscrews and an orthodontic wire were digitally created. The PAs were placed between canines and lateral incisors and projected at 4, 7, and 10 mm height distances. After that, distalization forces were simulated between PA and IZC miniscrews. RESULTS: The anterior teeth deformation produced in the FEA models was assessed according to a Von Mises equivalent. The stress was measured, revealing tendencies of initial maxillary teeth movement. No differences were found between the right and left sides. However, there was a significant difference among models in the under-stress areas, especially the apical and cervical root areas of the maxillary anterior teeth. More significant extrusion and lingual tipping of incisors were observed with the 4 mm power arm compared to the 7 mm and 10 mm ones. The 10 mm power arm did not show any tendency for extrusion of maxillary central incisors but a tendency for buccal tipping and intrusion of lateral incisors. CONCLUSION: The maxillary incisors and canines have different immediate movement tendencies according to the height of the anterior point of the en-masse distalization force application. Based on the PA height increase, a change from lingual to buccal tipping and less extrusion tendency was observed for the incisors, while the lingual tipping and extrusion trend for canines increased.

Holographic spatial coherence analysis of a laser.

We show that the second-order coherent-mode representation of a stationary quasi-monochromatic scalar light beam can be experimentally characterized by dual-mode holographic interference using an arbitrary basis. Analysis of the laser beam emitted from a stable spherical mirror cavity, using a mismatched Hermite-Gaussian basis, recovered the profiles and powers of a set of cavity modes with the expected spot size, including a hybrid of frequency degenerate modes. Observed near- and far-field irradiance transverse profiles and associated M2 parameter measures confirmed the results.

The inhibitory effects of Curcuma longa L. essential oil and curcumin on Aspergillus flavus link growth and morphology.

The essential oil from Curcuma longa L. was analysed by GC/MS. The major components of the oil were ar-turmerone (33.2%), α -turmerone (23.5%) and ß -turmerone (22.7%). The antifungal activities of the oil were studied with regard to Aspergillus flavus growth inhibition and altered morphology, as preliminary studies indicated that the essential oil from C. longa inhibited Aspergillus flavus Link aflatoxin production. The concentration of essential oil in the culture media ranged from 0.01% to 5.0% v/v, and the concentration of curcumin was 0.01-0.5% v/v. The effects on sporulation, spore viability, and fungal morphology were determined. The essential oil exhibited stronger antifungal activity than curcumin on A. flavus. The essential oil reduced the fungal growth in a concentration-dependent manner. A. flavus growth rate was reduced by C. longa essential oil at 0.10%, and this inhibition effect was more efficient in concentrations above 0.50%. Germination and sporulation were 100% inhibited in 0.5% oil. Scanning electron microscopy (SEM) of A. flavus exposed to oil showed damage to hyphae membranes and conidiophores. Because the fungus is a plant pathogen and aflatoxin producer, C. longa essential oil may be used in the management of host plants.

Ocular Symptoms Associated with COVID-19 Are Correlated with the Expression Profile of Mouse SARS-CoV-2 Binding Sites.

The COVID-19 pandemic has engendered significant scientific efforts in the understanding of its infectious agent SARS-CoV-2 and of its associated symptoms. A peculiar characteristic of this virus lies in its ability to challenge our senses, as its infection can lead to anosmia and ageusia. While ocular symptoms, such as conjunctivitis, optic neuritis or dry eyes, are also reported after viral infection, they have lower frequencies and severities, and their functional development is still elusive. Here, using combined technical approaches based on histological and gene profiling methods, we characterized the expression of SARS-CoV-2 binding sites (Ace2/Tmprss2) in the mouse eye. We found that ACE2 was ectopically expressed in subtissular ocular regions, such as in the optic nerve and in the Harderian/intraorbital lacrimal glands. Moreover, we observed an important variation of Ace2/Tmprss2 expression that is not only dependent on the age and sex of the animal, but also highly heterogenous between individuals. Our results thus give new insight into the expression of SARS-CoV-2 binding sites in the mouse eye and propose an interpretation of the human ocular-associated symptoms linked to SARS-CoV-2.

Hybrid computational and real data-based positioning of small cells in 5G networks.

One of the key technologies in smart cities is the use of next generation networks such as 5G networks. Mainly because this new mobile technology offers massive connections in densely populated areas in smart cities, thus playing a crucial role for numerous subscribers anytime and anywhere. Indeed, all the most important infrastructure to promote a connected world is being related to next generation networks. Specifically, the small cells transmitters is one of the 5G technologies more relevant to provide more connections and to attend the high demand in smart cities. In this article, a smart small cell positioning is proposed in the context of a smart city. The work proposal aims to do this through the development of a hybrid clustering algorithm with meta-heuristic optimizations to serve users, with real data, of a region satisfying coverage criteria. Furthermore, the problem to be solved will be the best location of the small cells, with the minimization of attenuation between the base stations and its users. The possibilities of using multi-objective optimization algorithms based on bioinspired computing, such as Flower Pollination and Cuckoo Search, will be verified. It will also be analyzed by simulation which power values would allow the continuity of the service with emphasis on three 5G spectrums used around the world: 700 MHz, 2.3 GHz and 3.5 GHz.

Substrates of Opposite Polarities and Downstream Processing for Efficient Production of the Biosurfactant Mannosylerythritol Lipids from Moesziomyces spp.

Biosurfactants can replace fossil-driven surfactants with positive environmental impacts, owing to their low eco-toxicity and high biodegradability. However, their large-scale production and application are restricted by high production costs. Such costs can be reduced using renewable raw materials and facilitated downstream processing. Here, a novel strategy for mannosylerythritol lipid (MEL) production explores the combination of hydrophilic and hydrophobic carbon sources sideways with a novel downstream processing strategy, based on nanofiltration technology. Co-substrate MEL production by Moesziomyces antarcticus was threefold higher than using D-glucose with low levels of residual lipids. The use of waste frying oil instead of soybean oil (SBO) in co-substrate strategy resulted in similar MEL production. Moesziomyces antarcticus cultivations, using 3.9 M of total carbon in substrates, yields 7.3, 18.1, and 20.1 g/L of MEL, and 2.1, 10.0, and 5.1 g/L of residual lipids, for D-glucose, SBO, and a combination of D-Glucose and SBO, respectively. Such approach makes it possible to reduce the amount of oil used, offset by the equivalent molar increase in D-glucose, improving sustainability and decreasing residual unconsumed oil substrates, facilitating downstream processing. Moesziomyces spp. also produces lipases that broken down the oil and, thus, residual unconsumed oils are in the form of free fatty-acids or monoacylglycerol, which are smaller molecules than MEL. Therefore, nanofiltration of ethyl acetate extracts from co-substrate-based culture broths allows to improve MEL purity (ratio of MEL per total MEL and residual lipids) from 66 to 93% using 3-diavolumes.

Prognostic Assessment of Fractional Flow Reserve in Different Strata in Patients with Coronary Artery Disease. / Avaliação Prognóstica da Reserva de Fluxo Fracionada em Diferentes Estratos nos Pacientes com Doença Arterial Coronariana.

BACKGROUND: There are limited real-world data on the clinical course of untreated coronary lesions according to their functional severity. OBJECTIVE: To evaluate the 5-year clinical outcomes of patients with revascularized lesions with fractional flow reserve (FFR) ≤ 0.8 and patients with non-revascularized lesions with FFR > 0.8. METHODS: The FFR assessment was performed in 218 patients followed for up to 5 years. Participants were classified based on FFR into ischemia group (≤ 0.8, intervention group, n = 55), low-normal FFR group (> 0.8-0.9, n = 91), and high-normal FFR group (> 0.9, n = 72). The primary endpoint was major adverse cardiac events (MACEs), a composite of death, myocardial infarction, and need for repeat revascularization. The significance level was set at 0.05; therefore, results with a p-value < 0.05 were considered statistically significant. RESULTS: Most patients were male (62.8%) with a mean age of 64.1 years. Diabetes was present in 27%. On coronary angiography, the severity of stenosis was 62% in the ischemia group, 56.4% in the low-normal FFR group, and 54.3% in the high-normal FFR group (p<0.05). Mean follow-up was 3.5 years. The incidence of MACEs was 25.5%, 13.2%, and 11.1%, respectively (p=0.037). MACE incidence did not differ significantly between the low-normal and high-normal FFR groups. CONCLUSION: Patients with FFR indicative of ischemia had poorer outcomes than those in non-ischemia groups. There was no difference in the incidence of events between the low-normal and high-normal FFR groups. Long-term studies with a large sample size are needed to better assess cardiovascular outcomes in patients with moderate coronary stenosis with FFR values between 0.8 and 1.0.

FUNDAMENTO: Existem dados limitados sobre a evolução clínica de lesões coronarianas não tratadas de acordo com sua gravidade funcional no mundo real. OBJETIVO: Este estudo teve como objetivo avaliar os resultados clínicos de até 5 anos em pacientes com lesões revascularizadas com reserva de fluxo fracionada (FFR) ≤ 0,8 e em pacientes com lesões não revascularizadas com FFR > 0,8. MÉTODOS: A avaliação pelo FFR foi realizada em 218 pacientes seguidos por até 5 anos. Os participantes foram classificados com base na FFR no grupo isquêmico (≤ 0,8, grupo intervenção, n = 55), no grupo FFR normal-baixa (> 0,8-0,9, n = 91) e no grupo FFR normal-alta (> 0,9, n = 72). O desfecho primário foram eventos cardíacos adversos maiores (ECAMs), um composto de morte, infarto do miocárdio e necessidade de nova revascularização. O nível de significância adotado neste estudo foi alfa = 0,05; deste modo, resultados com valores de p < 0,05 foram considerados estatisticamente significativos. RESULTADOS: A maioria dos participantes era do sexo masculino (62,8%) com média de idade de 64,1 anos. Diabetes estava presente em 27%. À angiografia coronariana, a gravidade da estenose avaliada foi de 62%, 56,4% e 54,3% nos grupos isquêmico, FFR normal-baixa e FFR normal-alta, respectivamente (p < 0,05). O período médio de acompanhamento foi de 3,5 anos. A incidência ECAM foi de 25,5%, 13,2% e 11,1%, respectivamente (p = 0,037). Não houve diferença na incidência de ECAM entre os grupos FFR normal-baixa e FFR normal-alta (p = NS). CONCLUSÃO: Pacientes com FFR indicativa de isquemia apresentaram piores desfechos quando comparados aos dos grupos não isquêmicos. Entre os grupos que apresentaram valores de FFR considerados normal-baixo e normal-alto, não houve diferença na incidência de eventos. Há necessidade de estudos de longo prazo e com grande número de pacientes para melhor avaliar os desfechos cardiovasculares em pacientes portadores de estenose coronariana moderada com valores de FFR entre 0,8 e 1,0.

Cardiomyopathy Induced by Artificial Cardiac Pacing: To Whom, When, Why, and How? Insights on Heart Failure Development.

Coordinated and harmonic (synchronous) ventricular electrical activation is essential for better left ventricular systolic function. Intraventricular conduction abnormalities, such as left bundle branch block due to artificial cardiac pacing, lead to electromechanical "dyssynchronopathy" with deleterious structural and clinical consequences. The aim of this review was to describe and improve the understanding of all the processes connecting the several mechanisms involved in the development of artificially induced ventricular dyssynchrony by cardiac pacing, most known as pacing-induced cardiomyopathy (PiCM). The chronic effect of abnormal impulse conduction and nonphysiological ectopic activation by artificial cardiac pacing is suspected to affect metabolism and myocardial perfusion, triggering regional differences in the activation/contraction processes that cause electrical and structural remodeling due to damage, inflammation, and fibrosis of the cardiac tissue. The effect of artificial cardiac pacing on ventricular function and structure can be multifactorial, and biological factors underlying PiCM could affect the time and probability of developing the condition. PiCM has not been included in the traditional classification of cardiomyopathies, which can hinder detection. This article reviews the available evidence for pacing-induced cardiovascular disease, the current understanding of its pathophysiology, and reinforces the adverse effects of right ventricular pacing, especially right ventricular pacing burden (commonly measured in percentage) and its repercussion on ventricular contraction (reflected by the impact on left ventricular systolic function). These effects might be the main defining criteria and determining mechanisms of the pathophysiology and the clinical repercussion seen on patients.

Extreme bariatric endoscopy: stenting to reconnect the pouch to the gastrojejunostomy after a Roux-en-Y gastric bypass.

BACKGROUND: Among the possible complications of bariatric surgery, fistula and partial dehiscence of the gastric suture are well known. Reoperation often is required but results in significant morbidity. Endoscopic treatment of some bariatric complications is feasible and efficient. METHODS: A modified metallic stent was placed between the gastroaesophageal junction and the alimentary jejunal limb, allowing the passage of a nasoenteric feeding tube into the jejunal limb. RESULTS: Endoscopy showed disruption of nearly the entire staple line at the gastric pouch. The modified stent was placed and allowed wound healing. After 31 days, the stent had migrated and was removed endoscopically. Total closure of the fistula was reported 30 days afterward. CONCLUSIONS: Endoscopic treatment of some bariatric surgery complications is feasible and has been reported previously. This report presents a case of a serious leakage treated by placement of a self-expandable metal stent to bridge the fistula.

EUS-guided biliary drainage: a review article.

CONTEXT: To demonstrate a comprehensive review of published articles regarding EUS-guided biliary drainage. METHODS: Review of studies regarding EUS-guided biliary drainage including case reports, case series and previous reviews. RESULTS: EUS-guided hepaticogastrostomy, choledochoduodenostomy and choledochoantrostomy are advanced procedures on biliary and pancreatic endoscopy and together make up the echo-guided biliary drainage. Hepaticogastrostomy is indicated in cases of hilar obstruction, while the procedure of choice is choledochoduodenostomy in distal lesions. Both procedures must be done only after unsuccessful ERCP. The indication of these procedures must be made under a multidisciplinary view while sharing information with the patient or legal guardian. CONCLUSION: Hepaticogastrostomy and choledochoduodenostomy are feasible when performed by endoscopists with expertise in biliopancreatic endoscopy and advanced echo-endoscopy and should be performed currently under rigorous protocol in educational institutions.

[A novel endoscopic treatment of pancreas divisum]. / A novel endoscopic treatment of pancreas divisum.

Pancreas divisum is the most common congenital malformation of the pancreas that results from the non-fusion or incomplete fusion of the ventral and dorsal portions of the embryonic pancreas. It is found in 7% of autopsy studies (range 1-14%) and is generally asymptomatic. 5% of the patients have symptoms, which are basically cases of abdominal pain and recurrent pancreatitis. We report the case of a woman of 51 y, postcholecystectomy with 2 episodes of pancreatitis with imaging from magnetic resonance of pancreatic divisum with communication between the dorsal and ventral pancreas. We proceeded by endoscopy (ERCP) to dilate the major duct, and then made a minor duct papillotomy and made a hydropneumatic ball dilatation with the catheter balloon up the waist portion. The procedure was successful with 9 months of follow up.

[EUS-guided access to dorsal pancreatic duct in a patient with pancreas divisum].

Case report of pancreas divisum dorsal pancreatic duct access with endoscopic ultrasonography help to relief pain in a patient with not possible access by PCRE.

Extrahepatic anterograde covered self-expandable metallic stent placement across malignant biliary obstruction passed by endoscopic ultrasound guidance access: a challenging technique.

The authors report the case of a female patient submitted to endoscopic cholangiography intending to drain the biliary tree due to jaundice. The patient had gastrointestinal deviation due to an advanced gastric cancer that evolved with a distal extrahepatic mass. Abdominal CT scan demonstrated a distal mass, extrahepatic biliary dilation and a normal intra-hepatic tree. In this condition and after a multidisciplinary discussion, an endoscopic ultrasound guided extrahepatic access with the deployment of a partially covered self-expandable metallic stent was performed. The patient normalized her bilirubin levels after a successful procedure.

Dynamics of Panstrongylus megistus infestation, the primary vector of Trypanosoma cruzi in Minas Gerais, Brazil.

Panstrongylus megistus is considered one of the primary species of epidemiological importance for the transmission of Chagas disease in Brazil due to its wide geographical distribution throughout the national territory, good ability to invade and colonize houses, and high rates of natural infection by the Trypanosoma cruzi. The importance of this species in Minas Gerais has been recognized since the 80s. It is responsible for the high prevalence rates of Chagas disease in the west of the state. Studies conducted in the municipality of Jaboticatubas show that P. megistus is still the most captured vector in the region, even after 40 years of uninterrupted actions of the Chagas Disease Control Program in the municipality. Despite the importance of the species, its population dynamics is little studied. Consequently, crucial genetic information such as genetic diversity and gene flow among environments have not been well characterized yet. In this context, this work presents a population genetic analysis at the microgeographic level using microsatellite markers in samples of P. megistus obtained from Jaboticatubas to better understand the infestation dynamics of the primary vector species of T. cruzi in the region. The observed and expected heterozygosity ranged from 0.26 to 0.47 and 0.47 to 0.65, respectively. Most loci presented Hardy-Weinberg disequilibrium due to the excess of homozygotes. The pairwise Fst ranged from 0.05 to 0.35, with the p value significant for all comparisons, indicating the absence of gene flow between them. The values of Fis found ranged from 0.25 to 0.52, all values of p ≤ 0.05, probably due to structured populations, inbreeding, or null alleles. The results suggest an extended stay of this species in the colonized environments, with rare dispersal to other locations. Such results differ from that observed for Triatoma brasiliensis and Triatoma dimidiata, species that present constant movement, even after colonization of the artificial environment, and are similar to the behavior of Triatoma infestans. Thus, the study reinforces the importance of continuous entomological surveillance in the areas of occurrence of P. megistus to avoid the installation and formation of new foci of household infestation in the region.