Machine Learning-Based Optoacoustic Tissue Classification Method for Laser Osteotomes Using an Air-Coupled Transducer.

BACKGROUND AND OBJECTIVES: Using lasers instead of mechanical tools for bone cutting holds many advantages, including functional cuts, contactless interaction, and faster wound healing. To fully exploit the benefits of lasers over conventional mechanical tools, a real-time feedback to classify tissue is proposed. STUDY DESIGN/MATERIALS AND METHODS: In this paper, we simultaneously classified five tissue types-hard and soft bone, muscle, fat, and skin from five proximal and distal fresh porcine femurs-based on the laser-induced acoustic shock waves (ASWs) generated. For laser ablation, a nanosecond frequency-doubled Nd:YAG laser source at 532 nm and a microsecond Er:YAG laser source at 2940 nm were used to create 10 craters on the surface of each proximal and distal femur. Depending on the application, the Nd:YAG or Er:YAG can be used for bone cutting. For ASW recording, an air-coupled transducer was placed 5 cm away from the ablated spot. For tissue classification, we analyzed the measured acoustics by looking at the amplitude-frequency band of 0.11-0.27 and 0.27-0.53 MHz, which provided the least average classification error for Er:YAG and Nd:YAG, respectively. For data reduction, we used the amplitude-frequency band as an input of the principal component analysis (PCA). On the basis of PCA scores, we compared the performance of the artificial neural network (ANN), the quadratic- and Gaussian-support vector machine (SVM) to classify tissue types. A set of 14,400 data points, measured from 10 craters in four proximal and distal femurs, was used as training data, while a set of 3,600 data points from 10 craters in the remaining proximal and distal femur was considered as testing data, for each laser. RESULTS: The ANN performed best for both lasers, with an average classification error for all tissues of 5.01 ± 5.06% and 9.12 ± 3.39%, using the Nd:YAG and Er:YAG lasers, respectively. Then, the Gaussian-SVM performed better than the quadratic SVM during the cutting with both lasers. The Gaussian-SVM yielded average classification errors of 15.17 ± 13.12% and 16.85 ± 7.59%, using the Nd:YAG and Er:YAG lasers, respectively. The worst performance was achieved with the quadratic-SVM with a classification error of 50.34 ± 35.04% and 69.96 ± 25.49%, using the Nd:YAG and Er:YAG lasers. CONCLUSION: We foresee using the ANN to differentiate tissues in real-time during laser osteotomy. Lasers Surg. Med. © 2020 Wiley Periodicals LLC.

Air-Coupled Ultrasonic Probe Integrity Test Using a Focused Transducer with Similar Frequency and Limited Aperture for Contrast Enhancement.

Air-coupled ultrasonic probes require a special design approach and handling due to the significant mismatch to the air. Outer matching layers have to be soft so can be easily damaged and excitation voltages might cause the degradation of electrodes or bonding between the layers. Integrity inspection is desired during design, manufacturing, and exploitation. Spatial distribution of a transduction efficiency over piezoelement surface is proposed as a convenient means for the air-coupled probe integrity inspection. Focused transducer of similar center frequency is used to scan the surface of the inspected probe. However, such approach creates a challenge, i.e., area of the scanning beam is much smaller than the total receiving area of the inspected probe, therefore, contrast and imaging resolution are significantly degraded. Masking aperture made from cardboard and felt, placed at the focal point was proposed as solution. Far-range sidelobes were suppressed down to the noise floor (-50 dB) and the near-range sidelobes were reduced down to -17 dB. The proposed modification allows to use a similar frequency focused transducer. Probe integrity inspection can be carried out at significantly enhanced contrast and lateral resolution. Natural and artificial defects can be detected by the use of the proposed method.

Ultrasonic Sensing of Plant Water Needs for Agriculture.

Fresh water is a key natural resource for food production, sanitation and industrial uses and has a high environmental value. The largest water use worldwide (~70%) corresponds to irrigation in agriculture, where use of water is becoming essential to maintain productivity. Efficient irrigation control largely depends on having access to reliable information about the actual plant water needs. Therefore, fast, portable and non-invasive sensing techniques able to measure water requirements directly on the plant are essential to face the huge challenge posed by the extensive water use in agriculture, the increasing water shortage and the impact of climate change. Non-contact resonant ultrasonic spectroscopy (NC-RUS) in the frequency range 0.1-1.2 MHz has revealed as an efficient and powerful non-destructive, non-invasive and in vivo sensing technique for leaves of different plant species. In particular, NC-RUS allows determining surface mass, thickness and elastic modulus of the leaves. Hence, valuable information can be obtained about water content and turgor pressure. This work analyzes and reviews the main requirements for sensors, electronics, signal processing and data analysis in order to develop a fast, portable, robust and non-invasive NC-RUS system to monitor variations in leaves water content or turgor pressure. A sensing prototype is proposed, described and, as application example, used to study two different species: Vitis vinifera and Coffea arabica, whose leaves present thickness resonances in two different frequency bands (400-900 kHz and 200-400 kHz, respectively), These species are representative of two different climates and are related to two high-added value agricultural products where efficient irrigation management can be critical. Moreover, the technique can also be applied to other species and similar results can be obtained.

Air-coupled piezoelectric transducers with active polypropylene foam matching layers.

This work presents the design, construction and characterization of air-coupled piezoelectric transducers using 1-3 connectivity piezocomposite disks with a stack of matching layers being the outer one an active quarter wavelength layer made of polypropylene foam ferroelectret film. This kind of material has shown a stable piezoelectric response together with a very low acoustic impedance (<0.1 MRayl). These features make them a suitable candidate for the dual use or function proposed here: impedance matching layer and active material for air-coupled transduction. The transducer centre frequency is determined by the l/4 resonance of the polypropylene foam ferroelectret film (0.35 MHz), then, the rest of the transducer components (piezocomposite disk and passive intermediate matching layers) are all tuned to this frequency. The transducer has been tested in several working modes including pulse-echo and pitch-catch as well as wide and narrow band excitation. The performance of the proposed novel transducer is compared with that of a conventional air-coupled transducers operating in a similar frequency range.

Ultrasonic needle hydrophone calibration in air by a parabolic off-axis mirror focused beam using three-transducer reciprocity.

An acoustic field distribution investigation in air requires a small receiving sensor. Needle hydrophones seem to be an attractive solution, and it has previously been demonstrated that needle hydrophones designed for use in water can be used in air. The metrology problem is that an absolute sensitivity calibration is needed, because needle hydrophones are not characterized in air, especially for frequencies below 1 MHz, which is of interest for air-coupled ultrasound. Conventional, three-transducer/microphone reciprocity calibration requires measurements to be done in the far field. However, when transducer diameter is large and the frequency is high, the required measurement distance becomes very large: 3 m for a 20 mm source, transmitting at 1 MHz. Large propagation distance leads to high attenuation and nonlinear effects in air propagation, and distortion and losses accumulate. Small needle hydrophones have low sensitivity, so that high excitation amplitudes would be required, which can lead to transducer heating and increase nonlinearity effects. A derivative of the three-transducer reciprocity calibration method is proposed, where a large aperture transducer is focused onto a hydrophone, using hybrid of plane wave and spherical wave reciprocity. Use of a focused source minimizes the frequency-dependent diffraction effects, and the spherical wave approximation is valid at the focal distance, and low level excitation signals can be used. Focusing is accomplished using a parabolic off-axis mirror. Calibration is in transmission, which reduces the complexity of the electrical measurements. The corresponding equations have been derived for this setup. Calibration of the transducer and needle hydrophone absolute sensitivity is obtained.

Novel acoustic radiation force optical coherence elastography based on ultrasmall ultrasound transducer for biomechanics evaluation of in vivo cornea.

We developed a novel acoustic radiation force optical coherence elastography (ARF-OCE) based on an ultrasmall ultrasound transducer for quantitative biomechanics evaluations of in vivo cornea. A custom single-sided meta-ultrasonic transducer with an outer diameter of 1.8 mm, focal spot diameter of 1.6 mm, central frequency of 930 kHz, and focal length of 0.8 mm was applied to excite the sample. The sample arm of the ARF-OCE system employed a three-dimensional printed holder that allowed for ultrasound excitation and ARF-OCE detection. The phase-resolved algorithm was combined with a Lamb wave model to depth-resolved evaluate corneal biomechanics after keratoconus and cross-linking treatments (CXL). The results showed that, compare to the healthy cornea, the Lamb wave velocity was significantly reduced in the keratoconus, increased in the cornea after CXL, and increased with cross-linked irradiation energy in the cornea. These results indicated the good clinical translation potential of the proposed novel ARF-OCE.

Assessment of Textural Properties of Puffed Corn Cakes during Storage at Different Relative Humidity.

Moisture adsorption is considered a critical factor during production and shelf-life of puffed corn cakes (PCC). This study aims to develop and validate an instrumental method and a mathematical model for the characterization of the textural modifications caused by the moisture adsorption in PCC. For that purpose, PCC were stored at different relative humidities to achieve a wide range of water activities (from 0.1 to 0.8 at 22 ± 1 °C). A flexion-compression test was successfully validated in order to characterize the average textural properties of a PCC batch. A mathematical model considering consecutive elastic and plastic zones satisfactorily fitted (average VAR 99.65% and MRE 3.29%) the average stress-strain profiles of PCC and reported useful textural parameters, such as the deformability modulus (E), critical strain (εc), and n curvature parameter. The structural modifications caused by moisture adsorption led to the reduction in E and n and the increase in εc. Even minor changes on the PCC moisture content involve remarkable modifications of the textural properties, which has to be considered for industry and retail distribution.

Modification of Mechanical and Electromechanical Resonances of Cellular Ferroelectret Films Depending on the External Load.

Ferroelectret films are cellular polymers with electrically charged pores that exhibit piezoelectric response. Among other applications, ferroelectret films have been widely used as active elements in air-coupled ultrasonic transducers. More recently, they have also been tested in water immersion. They show a promising wide frequency band response, but a poor sensitivity produced by the disappearance of the electromechanical resonances. This paper studies in detail the modification of FE films response when put into water immersion, both the mechanical and the electromechanical responses (the latter in transmission and reception modes). The lack of electromechanical thickness resonances when the films are put into water is explained as the result of the different profile of the modification of the polarization vector along the film thickness imposed by the large mechanical load produced by the water. This different electromechanical response can also be the reason for the subtle modification of the mechanical thickness resonances that is also observed and analyzed.

Fast and non-destructive ultrasonic test for face masks.

As a consequence of the large demand of face masks due to the COVID19 pandemic, cheap, fast and non-destructive tests that can verify in-line the variability of the filtration capacities, prove the potential disinfection and/or evaluate the performance of new filtering materials are needed. Using two different approaches based on air-coupled ultrasounds (0.15-1.6 MHz) with equivalent results, this work shows that each face mask presents a distinctive ultrasonic signature that enables the classification and the evaluation of their performance. Moreover, it is shown that the ultrasonic propagation through the face masks and the main filter layers takes place through the pore space and that low frequency response of the attenuation and the velocity is highly dispersive and is dominated by the interaction between the air in the pores and the fibers in the filters. Hence, the parameters that describe ultrasonic velocity, attenuation and dispersion can be related with their filtration efficiency and breathability. These techniques are fully contactless, non-invasive and fast.

Air-coupled broadband ultrasonic spectroscopy as a new non-invasive and non-contact method for the determination of leaf water status.

The implementation of non-destructive methods for the study of water changes within plant tissues and/or organs has been a target for some time in plant physiology. Recent advances in air-coupled ultrasonic spectroscopy have enabled ultrasonic waves to be applied to the on-line and real-time assessment of the water content of different materials. In this study, this technique has been applied as a non-destructive, non-invasive, non-contact, and repeatable method for the determination of water status in Populusxeuramericana and Prunus laurocerasus leaves. Frequency spectra of the transmittance of ultrasounds through plant leaves reveal the presence of at least one resonance. At this resonant frequency, transmittance is at its maximum. This work demonstrates that changes in leaf relative water content (RWC) and water potential (Psi) for both species can be accurately monitored by the corresponding changes in resonant frequency. The differential response found between both species may be due to the contrasting leaf structural features and the differences found in the parameters derived from the P-V curves. The turgor loss point has been precisely defined by this new technique, as it is derived from the lack of significant differences between the relative water content at the turgor loss point (RWC(TLP)) obtained from P-V curves and ultrasonic measurements. The measurement of the turgor gradient between two different points of a naturally transpiring leaf is easily carried out with the method introduced here. Therefore, such a procedure can be an accurate tool for the study of all processes where changes in leaf water status are involved.

Instantaneous and non-destructive relative water content estimation from deep learning applied to resonant ultrasonic spectra of plant leaves.

BACKGROUND: Non-contact resonant ultrasound spectroscopy (NC-RUS) has been proven as a reliable technique for the dynamic determination of leaf water status. It has been already tested in more than 50 plant species. In parallel, relative water content (RWC) is highly used in the ecophysiological field to describe the degree of water saturation in plant leaves. Obtaining RWC implies a cumbersome and destructive process that can introduce artefacts and cannot be determined instantaneously. RESULTS: Here, we present a method for the estimation of RWC in plant leaves from non-contact resonant ultrasound spectroscopy (NC-RUS) data. This technique enables to collect transmission coefficient in a [0.15-1.6] MHz frequency range from plant leaves in a non-invasive, non-destructive and rapid way. Two different approaches for the proposed method are evaluated: convolutional neural networks (CNN) and random forest (RF). While CNN takes the entire ultrasonic spectra acquired from the leaves, RF only uses four relevant parameters resulted from the transmission coefficient data. Both methods were tested successfully in Viburnum tinus leaf samples with Pearson's correlations between 0.92 and 0.84. CONCLUSIONS: This study showed that the combination of NC-RUS technique with deep learning algorithms is a robust tool for the instantaneous, accurate and non-destructive determination of RWC in plant leaves.

In-vivo 3D corneal elasticity using air-coupled ultrasound optical coherence elastography.

Corneal elasticity can resist elastic deformations under intraocular pressure to maintain normal corneal shape, which has a great influence on corneal refractive function. Elastography can measure tissue elasticity and provide a powerful tool for clinical diagnosis. Air-coupled ultrasound optical coherence elastography (OCE) has been used in the quantification of ex-vivo corneal elasticity. However, in-vivo imaging of the cornea remains a challenge. The 3D air-coupled ultrasound OCE with an axial motion artifacts correction algorithm was developed to distinguish the in-vivo cornea vibration from the axial eye motion in anesthetized rabbits and visualize the elastic wave propagation clearly. The elastic wave group velocity of in-vivo rabbit cornea was measured to be 5.96 ± 0.55 m/s, which agrees with other studies. The results show the potential of 3D air-coupled ultrasound OCE with an axial motion artifacts correction algorithm for quantitative in-vivo assessment of corneal elasticity.

Characterization of nanoparticles doped composites using ultrasound.

The aim of this work is the non-destructive automatic mechanical characterization of nanoparticles doped composites using ultrasound in order to understand and control the dispersion of the dopant nanoparticles in the final product. We present a method which is able to measure the elastic constants of composites (Youngs, Bulk, Shear Modulus and Poissons ratio), in addition to other parameters as density, sound velocity and thickness, providing information of the nanoparticles dispersion in the samples. All results are obtained with a single ultrasonic measure at each point of the samples' surface in an immersion setup with both pulse-echo and through-transmission measurements simultaneously, obtaining detailed information for all the samples' surface in a XY scanning. All the analysis is performed automatically, that is, no manual correction or adjustment is needed at any stage of the process. To validate the results, a polyester based resin has been analyzed with different concentrations of graphene nanoparticles as dopant. The method has shown to be very accurate and reliable. The resolution of the values obtained for the elastic constants is limited by the resolution in the velocities measurements, for which we have achieved a resolution in the order of cm/s, thus providing very accurate measurements of the elastic constants.

Passive focusing techniques for piezoelectric air-coupled ultrasonic transducers.

This paper proposes a novel passive focusing system for Air-Coupled Ultrasonic (ACU) piezoelectric transducers which is inspired by the Newtonian-Cassegrain (NC) telescope concept. It consist of a primary spherical mirror with an output hole and a flat secondary mirror, normal to the propagation axis, that is the transducer surface itself. The device is modeled and acoustic field is calculated showing a collimated beam with a symmetrical focus. A prototype according to this design is built and tested with an ACU piezoelectric transducer with center frequency at 400 kHz, high-sensitivity, wideband and 25 mm diameter flat aperture. The acoustic field is measured and compared with calculations. The presented prototype exhibit a 1.5 mm focus width and a collimated beam up to 15 mm off the output hole. In addition, the performance of this novel design is compared, both theoretically and experimentally, with two techniques used before for electrostatic transducers: the Fresnel Zone Plate - FZP and the off-axis parabolic or spherical mirror. The proposed NC arrangement has a coaxial design, which eases the transducers positioning and use in many applications, and is less bulky than off-axis mirrors. Unlike in off-axis mirrors, it is now possible to use a spherical primary mirror with minimum aberrations. FZP provides a more compact solution and is easy to build, but presents some background noise due to interference of waves diffracted at out of focus regions. By contrast, off-axis parabolic mirrors provide a well defined focus and are free from background noise, although they are bulky and more difficult to build. Spherical mirrors are more easily built, but this yields a non symmetric beam and a poorly defined focus.

Acoustic impedance matching of piezoelectric transducers to the air.

The purpose of this work is threefold: to investigate material requirements to produce impedance matching layers for air-coupled piezoelectric transducers, to identify materials that meet these requirements, and to propose the best solution to produce air-coupled piezoelectric transducers for the low megahertz frequency range. Toward this end, design criteria for the matching layers and possible configurations are reviewed. Among the several factors that affect the efficiency of the matching layer, the importance of attenuation is pointed out. A standard characterization procedure is applied to a wide collection of candidate materials to produce matching layers. In particular, some types of filtration membranes are studied. From these results, the best materials are identified, and the better matching configuration is proposed. Four pairs of air-coupled piezoelectric transducers also are produced to illustrate the performance of the proposed solution. The lowest two-way insertion loss figure is -24 dB obtained at 0.45 MHz. This increases for higher frequency transducers up to -42 dB at 1.8 MHz and -50 at 2.25 MHz. Typical bandwidth is about 15-20%.

A nondestructive integrity test for membrane filters based on air-coupled ultrasonic spectroscopy.

This work describes the application of an ultrasonic air-coupled characterization technique to membrane filters. Coefficient of transmission of sound at normal incidence through each membrane in the frequency range 0.55 MHz-2.4 MHz was measured. For all cases, at least one thickness resonance was observed. From these measurements density, velocity, and attenuation of ultrasonic longitudinal waves are calculated and compared to available filtration data such as water flux measurements and bubble point data, both provided by manufacturers. Results show that velocity of ultrasonic waves in membrane filters depends on the membrane grade and can be correlated to filtration properties; attenuation per wavelength is independent of membrane grade but sensitive to moisture content. Advantages of this technique over other conventional membrane tests are pointed out.

Influence of material structure on air-borne ultrasonic application in drying.

This work aims to contribute to the understanding of how the properties of the material being dried affect air-borne ultrasonic application. To this end, the experimental drying kinetics (40°C and 1m/s) of cassava (Manihot esculenta) and apple (Malus domestica var. Granny Smith) were carried out applying different ultrasonic powers (0, 6, 12, 19, 25 and 31 kW/m(3)). Furthermore, the power ultrasound-assisted drying kinetics of different fruits and vegetables (potato, eggplant, carrot, orange and lemon peel) already reported in previous studies were also analyzed. The structural, textural and acoustic properties of all these products were assessed, and the drying kinetics modeled by means of the diffusion theory. A significant linear correlation (r>0.95) was established between the identified effective diffusivity (DW) and the applied ultrasonic power for the different products. The slope of this relationship (SDUP) was used as an index of the effectiveness of the ultrasonic application; thus the higher the SDUP, the more effective the ultrasound application. SDUP was well correlated (r ⩾ 0.95) with the porosity and hardness. In addition, SDUP was largely affected by the acoustic impedance of the material being dried, showing a similar pattern with the impedance than the transmission coefficient of the acoustic energy on the interface. Thus, soft and open-porous product structures exhibited a better transmission of acoustic energy and were more prone to the mechanical effects of ultrasound. However, materials with a hard and closed-compact structure were less affected by acoustic energy due to the fact that the significant impedance differences between the product and the air cause high energy losses on the interface.

Monitoring plant response to environmental stimuli by ultrasonic sensing of the leaves.

Described here is the application of a technique based on the excitation, sensing and spectral analysis of thickness resonances of plant leaves using air-coupled and wide-band ultrasound pulses (150-900 kHz) to monitor variations in leaf properties caused by plant responses to different environmental stimuli, such as a sudden variation in light intensity (from 2000 to 150 µmol m(-2) s(-1)), sudden watering after a drought period, and along the diurnal cycle (3-5 days, with continuous variation in light intensity from 150 to 2000 µmol m(-2) s(-1) and change in temperature of about 5°C). Four different widely available species, both monocots and dicots and evergreen and deciduous, with different leaf features (shape, size, thickness, flatness, vascular structure), were selected to test the technique. After a sudden decrease in light intensity, and depending on the species, there was a relative increase in the thickness resonant frequency from 8% to 12% over a 25- to 50-min period. After sudden watering, the relative increase in the resonant frequency varied from 5% to 30% and the period from 10 to 400 min. Finally, along the diurnal cycle, the measured relative variation is between 4% and 10%. The technique revealed differences in both the amplitude of the frequency oscillations and the kinetics of the leaf response for different species and also within the same species, but for specimens grown under different conditions that present different cell structures at the tissue level. The technique can be equally applied to the leaves of any species that present thickness resonances.

Air-coupled ultrasonic resonant spectroscopy for the study of the relationship between plant leaves' elasticity and their water content.

Air-coupled wideband ultrasonic piezoelectric transducers are used in the frequency range 0.3 to 1.3 MHz to excite and sense first-order thickness resonances in the leaves of four different tree species at different levels of hydration. The phase and magnitude spectra of these resonances are measured, and the inverse problem solved; that is, leaf thickness and density, ultrasound velocity, and the attenuation coefficient are obtained. The elastic constant in the thickness direction (c33) is then determined from density and velocity data. The paper focuses on the study of c33, which provides a unique, fast, and noninvasive ultrasonic method to determine leaf elasticity and leaf water content.

Simultaneous determination of the ultrasound velocity and the thickness of solid plates from the analysis of thickness resonances using air-coupled ultrasound.

A method that combines transmission of air-coupled ultrasound pulses through solid plates and amplitude and phase spectral analysis is presented. In particular, the method analyzes the first thickness resonance of the plates. The purpose is to determine, simultaneously, velocity and attenuation coefficient of the ultrasounds in the material and the thickness of the plate. This is especially useful when thickness can not be measured independently. The method is successfully applied to soft membranes, biological samples and FRP composites.