Adipose tissue uncoupling protein 1 levels and function are increased in a mouse model of developmental obesity induced by maternal exposure to high-fat diet.

With brown adipose tissue (BAT) becoming a possible therapeutic target to counteract obesity, the prenatal environment could represent a critical window to modify BAT function and browning of white AT. We investigated if levels of uncoupling protein 1 (UCP1) and UCP1-mediated thermogenesis are altered in offspring exposed to prenatal obesity. Female CD-1 mice were fed a high-fat (HF) or standard-fat (SF) diet for 3 months before breeding. After weaning, all pups were placed on SF. UCP1 mRNA and protein levels were quantified using quantitative real-time PCR and Western blot analysis, respectively, in brown (BAT), subcutaneous (SAT) and visceral (VAT) adipose tissues at 6 months of age. Total and UCP1-dependent mitochondrial respiration were determined by high-resolution respirometry. A Student's t-test and Mann-Whitney test were used (significance: P<0.05). UCP1 mRNA levels were not different between the HF and SF offspring. UCP1 protein levels, total mitochondrial respiration and UCP1-dependent respiration were significantly higher in BAT from HF males (P=0.02, P=0.04, P=0.005, respectively) and females (P=0.01, P=0.04, P=0.02, respectively). In SAT, the UCP1 protein was significantly lower in HF females (P=0.03), and the UCP1-dependent thermogenesis was significantly lower from HF males (P=0.04). In VAT, UCP1 protein levels and UCP1-dependent respiration were significantly lower only in HF females (P=0.03, P=0.04, respectively). There were no differences in total respiration in SAT and VAT. Prenatal exposure to maternal obesity leads to significant increases in UCP1 levels and function in BAT in offspring with little impact on UCP1 levels and function in SAT and VAT.

The ophthalmo-meningeal foramen masquerading as an intraocular foreign body.

Although the diagnosis of intraocular foreign body is primarily a clinical one, radiographic imaging is often used to clarify the diagnosis and to localise the foreign body. For this case the radiographic findings served to confuse the diagnosis.

A comparison of the standard 12-lead electrocardiogram to exercise electrode placements.

In order to determine the effect of electrode placement and standing on the 12-lead electrocardiogram required prior to exercise testing, 104 male patients with stable coronary heart disease were studied. Electrocardiograms were recorded with two different placements of the arm electrodes commonly used for exercise testing with the patient supine and standing. These were compared to a standard ECG with the electrodes placed at the wrists and ankles with the patient supine. The four ECGs gathered on each patient were analyzed using standard visual techniques for diagnostic changes and using a computer for analysis of axis, amplitudes, and durations. There were important differences between the standard 12-lead ECG and the ECGs gathered with the pre-exercise test modifications. These differences were minimized by placing the arm electrodes as close to the shoulders as possible and by recording the ECG with the patient supine.

Prevalence of leptospirosis in Belize.

In a search for leptospirosis in Belize, at least one current case was diagnosed among 11 febrile hospital patients examined in a 2-week period in mid-1984. Sera from 440 survey subjects in 6 schools, 4 urban communities and 9 rural communities were examined for leptospiral antibodies by the microscopic agglutination test. 20 of 174 (11.5%) school pupils, 19 of 88 (22%) urban community subjects and 66/178 (37%) rural community subjects were positive at titres greater than or equal to 1:50, and 7.5%, 17% and 26%, respectively, were positive at greater than or equal to 1:100. Among all groups of survey subjects, serological reactions to antigens in the Australis serogroup predominated. Up to 81% of 155 sera from cattle in various areas of Belize were positive at titres greater than or equal to 1:100. Serological reactions to serogroup Sejroe predominated (43%), followed by Pyrogenes (16%) and Australis (15%). 20 of 71 (28%) pig sera were positive at greater than or equal to 1:100, predominantly to serovars in the Australis serogroup (51%). Of 162 sheep or goat sera examined, 65 (40%) were positive at greater than or equal to 1:100, mainly to serogroup Autumnalis (25%), followed by Australis (15%).

Education for navigating "permanent white water:" establishing a bachelors program for Canadian health services managers.

A rapidly changing health services environment challenges educators to critically assess what they do, and what they should do, to prepare managers to survive and grow professionally and to contribute to clients, organizations and the health system. In this paper we describe the establishment of an undergraduate degree-completion program in Health Services Management at Ryerson Polytechnic University in Toronto, Canada. In contrast to traditional programs which begin the process of education as the basis for later practice, this program, the first of its kind in a Canadian university, is designed to develop management skills and competencies on an existing base of professional experience and practice; it offers practitioners with 3-year diplomas in a health technology or allied health field the opportunity to complete an undergraduate degree on a part-time basis. Sections of the paper outline the multidisciplinary and multi-stage process of program development which took place outside of traditional departmental lines and involved a partnership of academics and practitioners; and the curriculum design, which integrates knowledge specific to the field of health administration, relevant knowledge from other professional fields, and liberal studies. In a final section we discuss the ongoing development of the program and factors contributing to its success.

Imageless computer navigation without pre-operative templating may lead to malpreparation of the femoral head in hip resurfacing.

The computed neck-shaft angle and the size of the femoral component were recorded in 100 consecutive hip resurfacings using imageless computer-navigation and compared with the angle measured before operation and with actual component implanted. The reliability of the registration was further analysed using ten cadaver femora. The mean absolute difference between the measured and navigated neck-shaft angle was 16.3 degrees (0 degree to 52 degrees). Navigation underestimated the measured neck-shaft angle in 38 patients and the correct implant size in 11. Registration of the cadaver femora tended to overestimate the correct implant size and provided a low level of repeatability in computing the neck-shaft angle. Prudent pre-operative planning is advisable for use in conjunction with imageless navigation since misleading information may be registered intraoperatively, which could lead to inappropriate sizing and positioning of the femoral component in hip resurfacing.

Effect of water stress on the chloroplast antioxidant system: I. Alterations in glutathione reductase activity.

The effect of water stress on glutathione reductase and catalase activities was evaluated in leaf blades of field-grown winter wheat (Triticum aestivum L.). Wheat was sown at two seeding rates under both irrigated and dryland conditions. Flag leaves from dryland plants sown at 60 kilograms/hectare showed no change in either glutathione reductase or catalase activities per unit leaf area, while leaves from the basal portion of the canopy exhibited a 273% increase in glutathione reductase activity and a 60% increase in catalase activity. Glutathione reductase activity in dryland plants sown at 120 kilograms/hectare increased 25% in flag leaves and 225% in basal leaves. No change in catalase activity was observed in either flag or basal leaves from these same plants. The increase in glutathione reductase activity in response to water stress was observed when activity was expressed on either a per unit leaf area, protein, or chlorophyll basis. No change in catalase activity was detected when enzyme activity was expressed on a protein basis.

Inhibition of carotenoid accumulation and abscisic acid biosynthesis in fluridone-treated dark-grown barley.

Treatment of dark-grown barley with 0.1 mM fluridone inhibited carotenoid accumulation but did not alter plastid biogenesis. Plastids isolated from dark-grown control and dark-grown fluridone-treated plants were similar in size and protein compositions. Dehydration of dark-grown control barley caused abscisic acid levels to increase 30-40-fold in 4 h, while plants treated with 0.1 mM fluridone accumulated very little abscisic acid in response to dehydration. These results suggest that fluridone-treated plants do not accumulate abscisic acid because of carotenoid deficiency rather than plastid dysfunction. Dark-grown barley plants treated with 0.31 microM fluridone accumulated low levels of carotenoids. Dehydration of these plants resulted in a 4-8-fold increase in abscisic acid and a decrease in antheraxanthin, violaxanthin and neoxanthin, but no change in beta-carotene or lutein plus zeaxanthin levels. This result is consistent with the suggestion that xanthophylls are precursors to abscisic acid in dehydrated plants.

Blue light regulates the accumulation of two psbD-psbC transcripts in barley chloroplasts.

Synthesis of D2, a Photosystem II reaction center protein encoded by psbD, is differentially maintained during light-induced chloroplast maturation. The continued synthesis of D2 is paralleled by selective light-induced accumulation of two psbD-psbC transcripts which share a common 5' terminus. In the present study, we examine the nature of the photoreceptor and the fluence requirement for psbD-psbC transcript induction. The light-induced change in psbD-psbC RNA population can be detected between 1 and 2 h after 4.5 day old dark-grown barley seedlings are transferred to the light. Light-induced transcript accumulation occurs normally in the chlorophyll-deficient barley mutant, xan-f10, indicating that light-activated chlorophyll formation and photosynthesis are not required for RNA induction. High fluence blue light fully induces psbD-psbC transcript accumulation; low or high fluence red or far-red light do not. However, psbD-psbC transcript accumulation elicited by blue light pulses can be partially attenuated if far-red light is given immediately following the blue light treatment. Thus, although blue light is needed to initiate transcript accumulation, phytochrome modulates the amplitude of the response. Pretreatment of dark-grown plants with cycloheximide blocks light-induced psbD-psbC transcript accumulation. This could implicate a blue-light responsive nuclear gene in the light-induced accumulation of the two psbD-psbC transcripts.

Translation and stability of proteins encoded by the plastid psbA and psbB genes are regulated by a nuclear gene during light-induced chloroplast development in barley.

We have characterized a nuclear mutant of barley, viridis-115, lacking photosystem II (PSII) activity and compared it to wild-type seedlings during light-induced chloroplast development. Chloroplasts isolated from wild-type and viridis-115 seedlings illuminated for 1 h synthesized similar polypeptides and had similar protein composition. After 16 h of illumination, however, mutant plastids exhibited reduced ability to radiolabel D1, CP47, and several low Mr membrane polypeptides, and by 72 h, synthesis of these proteins was undetectable. Immunoblot analysis showed that plastids of dark-grown wild-type barley lacked several PSII proteins (D1, D2, CP47, and CP43) and that 16 h of illumination resulted in the accumulation of these polypeptides. In contrast, these polypeptides did not accumulate in illuminated viridis-115 seedlings, although mutant plastids accumulated two PSII proteins that participate in oxygen evolution, oxygen-evolving enhancers 1 and 3. Northern analysis showed that the levels of psbA and psbB mRNA in mutant plastids were equal to or greater than levels in wild-type plastids throughout the developmental period examined here. These results indicate that the nuclear mutation present in viridis-115 affects the translation and stability of the chloroplast-encoded D1 and CP47 polypeptides and that its influence is expressed after the onset of light-induced chloroplast development.

Characterization of the barley chloroplast transcription units containing psaA-psaB and psbD-psbC.

Four plastid genes, psaA, psaB, psbD and psbC, were localized on the barley plastid genome. PsaA was adjacent to psaB in one transcription unit and psbD was adjacent to psbC in a second transcription unit. The transcription units containing psaA-psaB and psbD-psbC are separated by approximately 25 kbp on the barley plastid genome and are transcribed convergently. Transcripts hybridizing to each transcription unit were characterized by northern blot analysis, S1 protection experiments and primer extension analysis. Two 5.3 kb transcripts hybridize to psaA-psaB. The two transcripts have a common 5' end but differ at their 3' ends by about 26 nucleotides. The transcription unit which contains psbD-psbC also includes trnS(UGA), trnG(GCC), and an open reading frame which codes for a 62 amino acid protein. Six large transcripts ranging from 5.7 kb to 1.7 kb hybridize to the psbD-psbC transcription unit as well as several RNAs of tRNA size. The large transcripts arise from three 5' ends and two clusters of 3' ends. The 3' ends map near trnG(GCC) and trnS(UGA) and could be generated by RNA processing or termination of transcription. Two of the six transcripts hybridize to psbC but not psbD suggesting that translation of psbD and psbC could occur on separate RNAs.

Light-Dependent Accumulation of Radiolabeled Plastid-Encoded Chlorophyll a-Apoproteins Requires Chlorophyll a: I. Analysis of Chlorophyll-Deficient Mutants and Phytochrome Involvement.

The accumulation of radiolabeled plastid-encoded chlorophyll a-apoproteins is light dependent and is controlled at a posttranscriptional level. Illumination of dark-grown barley (Hordeum vulgare L.) with a brief pulse of red light induced the accumulation of radiolabeled chlorophyll a-apoproteins in subsequent protein synthesis assays. The induction of radiolabeled chlorophyll a-apoprotein accumulation was not affected by pretreatment of leaves with cycloheximide. Fluence response studies showed that a red light photoreceptor controls the accumulation of radiolabeled chlorophyll a-apoproteins with a threshold fluence of approximately 50 to 100 microeinsteins per square meter. While red light initiated chlorophyll a-apoprotein accumulation, this process was not reversed by a far red light treatment given immediately after the pulse of red light. The light pulse which initiated the accumulation of radiolabeled chlorophyll a-apoproteins also induced the rapid conversion of protochlorophyllide to chlorophyll a. A chlorophyll-deficient mutant, xan-f(10), which is blocked in chlorophyll biosynthesis prior to protochlorophyllide formation, failed to accumulate radiolabeled chlorophyll a-apoproteins in the light even though transcripts for these apoproteins were present. A second mutant, xan-j(64), which accumulates chlorophyllide in the light but only low levels of chlorophyll a, also showed reduced accumulation of radiolabeled chlorophyll a-apoproteins upon illumination. These results suggest that the light-induced conversion of protochlorophyllide to chlorophyll a is necessary for accumulation of the plastid-encoded chlorophyll a-apoproteins and one red light photoreceptor controlling this response is the protochlorophyllide holochrome.

Antibodies to some swine diseases in commercial piggeries in Central Zambia.

Blood samples were taken from 121 sows and gilts on 7 commercial piggeries located around Lusaka (Zambia). The samples tested negative for antibodies to Aujeszky's disease, transmissible gastroenteritis (TGE), swine influenza, hog cholera and brucellosis. Seventy-eight pigs from 5 farms had positive titres to porcine parvovirus. Eighteen sera showed positive titres to Leptospira celledoni.

Light-dependent changes in psbD and psbC transcripts of barley chloroplasts: accumulation of two transcripts maintains psbD and psbC translation capability in mature chloroplasts.

The psbD and psbC genes encode two polypeptides of Photosystem II. These genes are adjacent in the barley chloroplast genome and are part of a 5.7 kbp transcription unit. In dark-grown barley, four large transcripts hybridize to psbD and psbC; two additional transcripts hybridize to psbC. Illumination of 4.5-day-old dark-grown seedlings causes a decrease in the six psbD--psbC transcripts found in etioplasts and the accumulation of two different transcripts of 4.0 and 3.2 kb which hybridize to psbD and psbC. The light-induced transcripts have a common 5' end approximately 600 nt upstream of psbD and 3' ends 1175 and 175 nt downstream of psbC. The shift in psbD--psbC transcript population occurs during a phase of chloroplast maturation when transcript levels and translation of chloroplast genes such as psaA--psaB and psbB decline approximately 3- to 5-fold. In contrast, translation of the psbD and psbC gene products declines to a lesser extent, suggesting that the light-induced accumulation of the 4.0 and 3.2 kb psbD--psbC transcripts is required to maintain psbD and psbC gene product translation in mature chloroplasts.

Two cases of Van Buchem's disease.

A brother and sister suffering from hyperostosis corticalis generalisata familiaris (van Buchem's disease) are described. Both presented in early adult life with signs and symptoms of raised intracranial pressure and underwent partial craniectomy. Following surgery normal intellectual function was maintained and both survived to old age. In later life one showed cerebellar deficit due to bony encroachment of the posterior cranial fossa, while the other had a spastic paraparesis due to spinal cord compression. Several other siblings were affected by this disease which appears to be transmitted as an autosomal recessive gene.

Transjugular liver biopsy: a review of 461 biopsies.

Transjugular liver biopsy was performed in a large series of patients for whom routine percutaneous biopsy was contraindicated; most of the patients had severe liver disease associated with coagulopathies or massive ascites. Of the 461 biopsies performed over a 7-year period, adequate specimens for histologic diagnosis were obtained in 425; in 14 (3.3%), the biopsy provided a false-negative result. Minor complications such as neck pain, hematoma at the puncture site, or pyrexia occurred in 79 patients (17.1%). Serious complications were encountered in six patients (1.3%) (two with cardiac arrhythmias; four with intraperitoneal hemorrhage following capsular perforation), with an overall mortality rate for the series of 0.22%. Modification of the technique--taking the biopsy with the catheter positioned centrally rather than wedged peripherally--has reduced the occurrence of capsular perforation without affecting the success rate. Transjugular liver biopsy is a valuable technique that provides diagnostic information in a high proportion of cases in which conventional percutaneous biopsy is contraindicated.

Plant morphological and biochemical responses to field water deficits: I. Responses of glutathione reductase activity and paraquat sensitivity.

The effects of water deficits on plant morphology and biochemistry were analyzed in two photoperiodic strains of field-grown cotton (Gossypium hirsutum L.). Plants grown under dryland conditions exhibited a 40 to 85% decrease in leaf number, leaf area index, leaf size, plant height, and total weight per plant. Gross photosynthesis decreased from 0.81 to 0.47 milligram CO(2) fixed per meter per second and the average midday water, osmotic, and turgor potentials decreased to -2.1, -2.4, and 0.3 megapascals, respectively.There was a progressive increase in glutathione reductase activity and in the cellular antioxidant system in the leaves of stressed plants compared to the irrigated controls. The stress-induced increases in enzyme activity occurred at all canopy positions analyzed.Irrigation of the dryland plots following severe water stress resulted in a 50% increase in leaf area per gram fresh weight in newly expanded leaves of both strains over the leaves which had expanded under the dryland conditions. Paraquat resistance (a relative measure of the cellular antioxidant system) decreased in the strain T25 following irrigation. Glutathione reductase activities remained elevated in the T25 and T185 leaves which were expanded fully prior to irrigation and in the leaves which expanded following the irrigation treatment.

Biochemical documentation of allelic variation of the I-E antigens of the d, k, p, r, and u haplotypes.

The extent of allelic variation of the E alpha and E beta polypeptide chains of the I-E antigens from the H-2d, H-2k, H-2p, H-2r, and H-2u haplotypes is described. E alpha and E beta chains were individually labeled with arginine or lysine and compared by tryptic peptide analysis. The results indicate minimum variability among the E alpha polypeptides encoded by the d, k, p, and r haplotypes. However, the Eu alpha chain differed significantly from the other allelic E alpha gene products. On the other hand, the E beta alleles demonstrated substantial variability with the Ed beta being notably less similar to the other alleles than they are to each other. These findings are consistent with a number of observations regarding the serology and functions of the I-E antigens.

Routine breast screening for women aged 65-69: results from evaluation of the demonstration sites.

Routine programme data and specially designed surveys from 3 demonstration sites were analysed to determine the implications of extending the NHS Breast Screening Programme (NHSBSP), to include routine invitations for women up to 69 years. All women aged 65-69 and registered with GPs in these areas received routine invitations for breast screening along with those aged 50-64. Overall uptake was 71% in women aged 65-69 compared with 78% in younger women, but was > or = 90% in both groups who had previously attended within 5 years. Recall rates were lower for older women, but with a higher positive predictive value for cancer. The percentages of invasive cancer in different prognostic categories were similar in the 2 age groups. Older women took no longer to screen than younger women. The costs per woman invited or per woman screened were also similar to those for women aged 50-64, whilst the cost per cancer detected was some 34% lower in older women. Breast screening is as cost effective for women aged 65-69 as for those aged 50-64, with a higher cancer detection rate balancing shorter life expectancy. The proposed extension to the national programme will have considerable workforce implications for the NHSBSP and require additional resources.