RESUMO
INTRODUCTION: Persistent hyperplastic primary vitreous (PHPV) involves a spectrum of congenital ocular abnormalities characterized by the presence of a vascular membrane behind the lens. Retinoblastoma is a life-threatening intraocular malignancy that can cause blindness, eye loss, or even death. PHPV and retinoblastoma are extremely rare prenatal diseases. CASE PRESENTATION: Here, we present a case of fetal PHPV with retinoblastoma diagnosed using prenatal ultrasound. The unilateral lenses were hyperechoic, and irregular echogenic bands between the lenses and posterior eye walls were observed. In cases where the blood flow signal continues in the band-shaped hyperechoic area, PHPV with retinoblastoma should be suspected. CONCLUSION: PHPV with retinoblastoma can be prenatally diagnosed.
Assuntos
Vítreo Primário Hiperplásico Persistente , Neoplasias da Retina , Retinoblastoma , Gravidez , Feminino , Humanos , Vítreo Primário Hiperplásico Persistente/diagnóstico por imagem , Vítreo Primário Hiperplásico Persistente/patologia , Retinoblastoma/diagnóstico por imagem , Retinoblastoma/patologia , Corpo Vítreo/diagnóstico por imagem , Corpo Vítreo/anormalidades , Corpo Vítreo/patologia , Neoplasias da Retina/diagnóstico por imagem , Neoplasias da Retina/patologia , Ultrassonografia Pré-NatalRESUMO
Here, we describe the synthesis and characterization of the two µ-hydrido models of [NiFe]H2ases prepared from a new type of H2 activation by the corresponding two µ-hydroxo models. In addition, another µ-hydrido model prepared by the anionic exchange between one of the two µ-hydrido models and KPF6 is also reported. Interestingly, the synthesis of the two µ-hydrido models from H2 activation represents the first functional modeling of the H2 activation catalyzed by the [NiFe]H2ase (Ni-SIr)I state to give the Ni-R state.
RESUMO
AIM: To investigate the protective effect of heme oxygenase-1 (HO-1) against H2O2-induced apoptosis in human ARPE-19 cells. METHODS: The lentiviral vector expressing HO-1 was prepared and transfected into apoptotic ARPE-19 cells induced by H2O2. Functional experiments including cell counting kit-8 (CCK-8) assay, flow cytometry (FCM) and mitochondrial membrane potential assay were conducted. RESULTS: The ultrastructure of ARPE-19 cells was observed using transmission electron microscope (TEM). It was found that exogenous HO-1 significantly ameliorated H2O2-induced loss of cell viability, apoptosis and intracellular levels of reactive oxygen species (ROS) in ARPE-19 cells. The overexpression of HO-1 facilitated the transfer of nuclear factor erythroid-2-related factor 2 (Nrf2) from cytoplasm to nucleus, which in turn upregualted expressions HO-1 and B-cell lymphoma-2 (Bcl-2). Furthermore, HO-1 upregulation further inhibited H2O2-induced release of cysteinyl aspartate specific proteinase-3 (caspase-3). CONCLUSION: Exogenous HO-1 protect ARPE-19 cells against H2O2-induced oxidative stress by regulating the expressions of Nrf2, HO-1, Bcl-2, and caspase-3.
RESUMO
The structural and functional modeling of the active site of [NiFe]-hydrogenases has been proved to be challenging to a great extent. Herein, we report the synthesis, structures, and some properties of the NiFe-based dicarbonyl, terminal hydride, and µ-hydroxo models for the active site of [NiFe]-hydrogenases.