RESUMO
Current strategies to control leishmaniasis are mainly based on chemotherapy. However, none of the available drugs can be considered to be ideal to treat this disease. Because of the hydrophobic nature and bioactivities of their components, essential oils (EOs) can be considered as important sources for developing agents against intracellular pathogens, such as Leishmania parasites. In this study, we report the chemical characterization, antileishmanial activities, and cytotoxicity effect of the EO from Pluchea carolinensis (Jacq.) G. Don. (Asteraceae). Chemical analysis revealed that EO from aerial part from P. carolinensis is composed of 44 compounds. The main component was selin-11-en-4α-ol, which made up 51.0%. In vitro antileishmanial studies showed that P. carolinensis EO inhibited the growth of promastigotes (IC50 = 24.7 ± 7.1 µg/mL) and amastigotes (IC50 = 6.2 ± 0.1 µg/mL) of Leishmania amazonensis, while cytotoxicity evaluation revealed fivefold higher values than those for the parasites. In a model of experimental cutaneous leishmaniasis in BALB/c mice, five doses of EO at 30 mg/kg by intralesional route demonstrated smaller lesion size and parasite burden (p < 0.05) compared with animals treated with Glucantime® and untreated mice. In conclusion, in vitro and in vivo results showed the potentialities of EO from P. carolinensis with the future possibility of a new alternative in the treatment for leishmaniasis. Copyright © 2017 John Wiley & Sons, Ltd.
Assuntos
Antiprotozoários/farmacologia , Asteraceae/química , Leishmaniose Cutânea/tratamento farmacológico , Óleos Voláteis/farmacologia , Animais , Feminino , Leishmania/efeitos dos fármacos , Leishmania mexicana/efeitos dos fármacos , Meglumina/farmacologia , Antimoniato de Meglumina , Camundongos , Camundongos Endogâmicos BALB C , Compostos Organometálicos/farmacologia , Folhas de Planta/químicaRESUMO
Chenopodium ambrosioides is an aromatic herb used by native people to treat parasitic diseases. The aim of this work is to compare the in vitro anti-leishmanial activity of the essential oil (EO) from C. ambrosioides and its major components (ascaridole, carvacrol and caryophyllene oxide) and study their mechanism of action and activity against a panel of microorganism. Antileishmanial activity and cytotoxicity of the EO and major components was study. In addition, experiments to elucidate the mechanism of action were perform and activities against other microorganisms (bacteria, fungi and protozoa) were evaluate. All products were active against promastigote and amastigote forms of Leishmania. Ascaridole exhibited the better antileishmanial activity and the EO the highest selectivity index. The exploration of the mechanism suggests that the products cause a breakdown of mitochondrial membrane potential and a modification of redox indexes. Only EO showed antiprotozoal effect against Plasmodium falciparum and Trypanosoma brucei; while no activity against bacteria and fungi was observed. Our results demonstrate the potentialities of EO in cellular and molecular system, which could be consider in future studies to develop new antileishmanial drugs with a wide anti-parasitic spectrum.
Assuntos
Chenopodium ambrosioides/química , Leishmania infantum/efeitos dos fármacos , Leishmania mexicana/efeitos dos fármacos , Mitocôndrias/efeitos dos fármacos , Óleos Voláteis/farmacologia , Animais , Candida albicans/efeitos dos fármacos , Quelantes/farmacologia , Citocromos c/efeitos dos fármacos , Citocromos c/metabolismo , Escherichia coli/efeitos dos fármacos , Feminino , Concentração Inibidora 50 , Leishmania infantum/ultraestrutura , Leishmania mexicana/ultraestrutura , Macrófagos Peritoneais/efeitos dos fármacos , Macrófagos Peritoneais/parasitologia , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos BALB C , Óleos Voláteis/química , Óleos Voláteis/isolamento & purificação , Óleos de Plantas/química , Óleos de Plantas/isolamento & purificação , Óleos de Plantas/farmacologia , Plasmodium falciparum/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Trichophyton/efeitos dos fármacos , Trypanosoma brucei brucei/efeitos dos fármacos , Trypanosoma cruzi/efeitos dos fármacosRESUMO
The search for new therapeutic agents from natural sources has been a constant for the treatment of diseases such as leishmaniasis. Herein, in vitro and in vivo pharmacological activities of pure major phenolic constituents (caffeic acid, chlorogenic acid, ferulic acid, quercetin, and rosmarinic acid) from Pluchea carolinensis against Leishmania amazonensis are presented. Pure compounds showed inhibitory activity against promastigotes (IC50 = 0.2-0.9 µg/mL) and intracellular amastigotes (IC50 = 1.3-2.9 µg/mL). Four of them were selected after testing against macrophages of BALB/c mice: caffeic acid, ferulic acid, quercetin, and rosmarinic acid, with selective indices of 11, 17, 10, and 20, respectively. Ferulic acid, rosmarinic acid, and caffeic acid controlled lesion size development and parasite burden in footpads from BALB/c experimentally infected mice, after five injections of compounds by intralesional route at 30 mg/kg every 4 days. Pure compounds from P. carolinensis demonstrated antileishmanial properties.
Assuntos
Antiprotozoários/farmacologia , Asteraceae/química , Leishmania mexicana/efeitos dos fármacos , Leishmaniose/tratamento farmacológico , Animais , Ácidos Cafeicos/farmacologia , Células Cultivadas , Cinamatos/farmacologia , Ácidos Cumáricos/farmacologia , Depsídeos/farmacologia , Feminino , Macrófagos/parasitologia , Camundongos , Camundongos Endogâmicos BALB C , Quercetina/análogos & derivados , Quercetina/farmacologia , Ácido RosmarínicoRESUMO
Leishmaniasis is a neglected tropical disease caused by Leishmania protozoa. There is currently no vaccine against leishmaniasis, and chemotherapy remains the only effective control. However, conventional drugs are toxic, expensive, and require long periods of treatment, and resistance to clinical chemotherapeutic agents is emerging. Recent research on plants has shown a successful approach to obtain new antileishmanial alternatives. Herein, the in vitro and in vivo effects of the essential oil from Bixa orellana seeds against Leishmania amazonensis were evaluated. A total of 73 compounds were detected by gas chromatography-mass spectrometry analysis, of which ishwarane (18.6%) and geranylgeraniol (9.1%) were the major components. The oil showed activity against intracellular amastigote form (IC50 = 8.5 µg/mL), while the cytotoxic concentration was sevenfold higher for the host cells. The ability of Bixa oil to control disease progression of established cutaneous leishmaniasis in BALB/c mice was demonstrated, after a treatment with 30 mg/kg by intraperitoneal administration over 14 days. The present study reports for the first time the antileishmanial potentialities of the essential oil from B. orellana.
Assuntos
Antiprotozoários/farmacologia , Bixaceae/química , Leishmania/efeitos dos fármacos , Leishmaniose Cutânea/tratamento farmacológico , Óleos Voláteis/farmacologia , Óleos de Plantas/farmacologia , Animais , Antiprotozoários/química , Feminino , Cromatografia Gasosa-Espectrometria de Massas , Macrófagos Peritoneais/efeitos dos fármacos , Macrófagos Peritoneais/parasitologia , Camundongos , Camundongos Endogâmicos BALB C , Óleos Voláteis/química , Óleos de Plantas/química , Sementes/químicaRESUMO
Leishmaniasis is one of the most important parasitic infections, but current treatments are unsatisfactory due to their toxicity, cost and resistance. Therefore, the development of new antileishmanial compounds is imperative. Many people who live in endemic areas use plants as an alternative to treat the disease. In this paper, we characterised the essential oil from Piper auritum, evaluated its cytotoxicity and determined its antileishmanial activity. The chromatogram obtained by gas chromatography revealed 60 peaks and we found that safrole was the most abundant compound, composing 87% of the oil. The oil was active against the promastigotes of Leishmania major, Leishmania mexicana, Leishmania braziliensis and Leishmania donovani with a favourable selectivity index against peritoneal macrophages from BALB/c mice. The Piper-oil inhibited the growing of intracellular amastigotes of L. donovani with an IC50 value of 22.3 +/- 1.8 microg/mL. This study demonstrates the usefulness of the essential oils as a promising alternative to treat leishmaniasis.
Assuntos
Antiprotozoários/farmacologia , Leishmaniose/tratamento farmacológico , Macrófagos Peritoneais/efeitos dos fármacos , Óleos Voláteis/farmacologia , Piper/química , Óleos de Plantas/farmacologia , Animais , Antiprotozoários/química , Antiprotozoários/isolamento & purificação , Feminino , Cromatografia Gasosa-Espectrometria de Massas , Camundongos , Camundongos Endogâmicos BALB C , Óleos Voláteis/química , Óleos Voláteis/isolamento & purificação , Testes de Sensibilidade Parasitária , Óleos de Plantas/química , Óleos de Plantas/isolamento & purificaçãoRESUMO
CONTEXT: Leishmaniasis is a widespread tropical infection caused by different species of Leishmania protozoa. There is no immunoprophylaxis (vaccination) available for Leishmania infections and conventional treatments are unsatisfactory; therefore antileishmanial drugs are urgently needed. Natural products are attractive due to their structural diversity. OBJECTIVE: The present work investigated the antileishmanial action of 21 species of plants. MATERIALS AND METHODS: Plants were collected and their hydroalcoholic extracts were screened against promastigotes and amastigotes of L. amazonensis. Their toxicity was also assayed against peritoneal macrophages from BALB/c mice. RESULTS: Five extracts showed significant growth inhibitory activity against promastigote form. Only the extracts from Bidens pilosa L. (Asteraceae) and Punica granatum L. (Punicaceae) inhibited the growth of intracellular amastigotes, with IC(50) values of 42.6 and 69.6 microg/mL, respectively. In addition, a low toxicity on macrophage from BALB/c mice was observed. DISCUSSION: The antiparasitic activities of B. pilosa and P. granatum have been reported against other parasitic agents and their actions can be the results of flavonoids present in the extracts. CONCLUSION: This study supports the importance of natural products as potential sources in the search for new antileishmanial drugs.
Assuntos
Antiparasitários/farmacologia , Antiprotozoários/farmacologia , Descoberta de Drogas , Leishmania mexicana/efeitos dos fármacos , Extratos Vegetais/farmacologia , Plantas Medicinais/química , Animais , Antiparasitários/toxicidade , Antiprotozoários/toxicidade , Bidens/química , Células Cultivadas , Cuba , Avaliação Pré-Clínica de Medicamentos , Concentração Inibidora 50 , Leishmaniose/tratamento farmacológico , Lythraceae/química , Macrófagos Peritoneais/efeitos dos fármacos , Medicina Tradicional , Camundongos , Camundongos Endogâmicos BALB C , Fitoterapia , Extratos Vegetais/toxicidade , Folhas de Planta/químicaRESUMO
Background: Leishmaniasis is a zoonotic disease caused by protozoan parasites from Leishmania genus. Currently, there are no effective vaccines available and the available therapies are far from ideal. In particular, the development of new therapeutic strategies to reduce the infection caused by Leishmania amazonensis could be considered desirable. Different plant-derived products have demonstrated antileishmanial activity, including the essential oil (EO) from Artemisia absinthium L. (EO-Aa), Asteraceae. Methods: In the present study, the EO-Aa formulated in nanocochleates (EO-Aa-NC) was investigated in vitro against intracellular amastigotes of L. amazonensis and non-infected macrophages from BALB/c mice. In addition, the EO-Aa-NC was also evaluated in vivo against on experimental cutaneous leishmaniasis, which body weight, lesion progression, and parasite load were determined. Results: EO-Aa-NC displayed IC50 values of 21.5 ± 2.5 µg/mL and 27.7 ± 5.6 µg/mL against intracellular amastigotes of L. amazonensis and non-infected peritoneal macrophage, respectively. In the animal model, the EO-Aa-NC (30 mg/kg/intralesional route/every 4 days 4 times) showed no deaths or weight loss greater than 10%. In parallel, the EO-Aa-NC suppressed the infection in the murine model by approximately 50%, which was statistically superior (p < 0.05) than controls and mice treated with EO-Aa. In comparison with Glucantime®, EO-Aa-NC inhibited the progression of infection as efficiently (p > 0.05) as administration of the reference drug. Conclusions: Encochleation of EO-Aa resulted in a stable, tolerable, and efficacious antileishmanial formulation, facilitating systemic delivery of EO, with increased activity compared to administration of the free EO-Aa. This new formulation shows promising potential to future studies aimed at a new therapeutic strategy to treat leishmaniasis.
RESUMO
To date there are no vaccines against Leishmania and chemotherapy remains the mainstay for the control of leishmaniasis. The drugs currently used for leishmaniasis therapy are significantly toxic, expensive, and result in a growing frequency of refractory infections. In this study, we evaluated the effect of combinations of the main components of essential oil from Chenopodium ambrosioides (ascaridole, carvacrol, and caryophyllene oxide) against Leishmaniaamazonensis. Anti-leishmanial effects of combinations of pure compounds were evaluated in vitro and the fractional inhibitory concentration (FIC) indices were calculated. BALB/c mice infected with L. amazonensis were treated with different concentrations of ascaridole-carvacrol combinations by intralesional doses every 4 days. Disease progression and parasite burden in infected tissues were determined. In vitro experiments showed a synergistic effect of the combination of ascaridole-carvacrol against promastigotes of Leishmania with a FIC index of 0.171, while indifferent activities were observed for ascaridole-caryophyllene oxide (FIC index=3.613) and carvacrol-caryophyllene oxide (FIC index=2.356) combinations. The fixed ratio method showed that a 1:4 ascaridole-carvacrol ratio produced a better anti-protozoal activity on promastigotes, lower cytotoxicity, and synergistic activity on intracellular amastigotes (FIC index=0.416). Significant differences (p<0.05) in lesion size and parasite burden were demonstrated in BALB/c mice experimentally infected and treated with the ascaridole-carvacrol combinations compared with control animals. Carvacrol showed significant higher anti-radical activity in the DPPH assay compared with caryophyllene oxide. Electron spin resonance spectroscopy in combination with spin trapping suggested the presence of carbon-centered radicals after activation of ascaridole by Fe(2+). The intensity of the signals is preferably decreased upon addition of carvacrol. The ascaridole-carvacrol combination could represent a future alternative to monotherapeutic anti-leishmanial agents.
Assuntos
Antiprotozoários/administração & dosagem , Leishmania/efeitos dos fármacos , Leishmaniose Cutânea/tratamento farmacológico , Monoterpenos/administração & dosagem , Peróxidos/administração & dosagem , Sesquiterpenos/administração & dosagem , Animais , Antiprotozoários/farmacologia , Chenopodium ambrosioides/química , Monoterpenos Cicloexânicos , Cimenos , Combinação de Medicamentos , Cálculos da Dosagem de Medicamento , Camundongos , Camundongos Endogâmicos BALB C , Óleos Voláteis/farmacologia , Fitoterapia , Óleos de Plantas/administração & dosagem , Óleos de Plantas/farmacologia , Sesquiterpenos PolicíclicosRESUMO
The marine organisms are a rich source of varied natural products with unique functionality. A variety of natural products of new molecular structures with diverse biological activities have been reported from marine flora and fauna for treatment and/or prevention of human diseases. The present review briefly illustrates current status of marine products as antiprotozoal agents. The in vitro and in vivo studies of marine algae, invertebrates and micro-organism against different protozoa parasites are included. The marine products studied, according to international criterions for selection of more promisory products in the different models reported, demonstrated their potentialities as antiprozoal agents. Herein, the interest of scientific community to search new alternatives from marine environment has been demonstrated.
Assuntos
Antiprotozoários/uso terapêutico , Produtos Biológicos/uso terapêutico , Infecções por Protozoários/tratamento farmacológico , Animais , Antiprotozoários/isolamento & purificação , Organismos Aquáticos , Produtos Biológicos/isolamento & purificação , Descoberta de Drogas , Humanos , Infecções por Protozoários/parasitologiaRESUMO
Natural products have long been providing important drug leads for infectious diseases. Leishmaniasis is a major health problem worldwide that affects millions of people especially in the developing nations. There is no immunoprophylaxis (vaccination) available for Leishmania infections, and conventional treatments are unsatisfactory; therefore, antileishmanial drugs are urgently needed. In this work, 48 alcoholic extracts from 46 Cuban plants were evaluated by an in vitro bioassay against Leishmania amazonensis. Furthermore, their toxicity was assayed against murine macrophage. The three most potent extracts against the amastigote stage of Leishmania amazonensis were from Hura crepitans, Bambusa vulgaris, and Simarouba glauca.
RESUMO
INTRODUCTION: Leishmaniasis is a disease caused by several species from Leishmania genus, which has been increasingly reported in the last few years. Several genetic, immunological factors and others related to this parasite have been associated to the outcome of the infection, and the occurrence of illness in varied clinical forms. All the aforementioned has an impact on the diagnostic method that should be used. METHODS: A basic and recent literature review was made, mainly focused on general aspects of leishmaniasis as epidemiological situation of disease, life cycle and transmission, vectors, clinical presentation and diagnosis; the latter shows information about the main methods used at present. The procedure followed by the Leishmania group at "Pedro Kourí" Institute of Tropical Medicine to support the diagnostic activities was presented as well. RESULTS: Updated practical information about the chosen topics was presented, with a practical guiding approach aimed at the health personnel that must face suspected leishmaniasis cases. Tables and figures summarized relevant aspects in an organized form, as well as the working algorithm of Institute concerning diagnosis was presented. The application of molecular diagnosis by this working team was particularly underlined. CONCLUSIONS: As leishmaniasis is still out of control, opportune diagnosis remains a must. All the methods provide useful information for taking decisions on clinical management, treatment and epidemiology of this parasitosis; hence, a working algorithm was submitted in our lab based on the most useful methods under our present conditions and experience.
Assuntos
DNA de Protozoário/análise , Leishmania/genética , Leishmaniose/diagnóstico , Leishmaniose/parasitologia , Humanos , MicroscopiaRESUMO
BACKGROUND: In the present study, an activity of Bixa orellana extract against Leishmania amazonensis was demonstrated. RESULT: Experimentally infected BALB/c mice were treated with B. orellana extract which showed a significant activity against promastigote and amastigote forms of L. amazonensis. CONCLUSION: This study supports the importance of natural sources as antileishmanial drugs.
Assuntos
Antiprotozoários/farmacologia , Antiprotozoários/uso terapêutico , Bixaceae/química , Leishmania/efeitos dos fármacos , Leishmaniose/tratamento farmacológico , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Anfotericina B/farmacologia , Animais , Peso Corporal/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Feminino , Concentração Inibidora 50 , Estágios do Ciclo de Vida/efeitos dos fármacos , Macrófagos Peritoneais/citologia , Macrófagos Peritoneais/parasitologia , Camundongos , Camundongos Endogâmicos BALB C , Distribuição AleatóriaRESUMO
OBJECTIVE: To evaluate the antileishmanial activity of different extracts from three Cuban Pluchea species. METHODS: In in vitro assays the IC(50) was calculated in the promastigotes and amastigotes forms as cytotoxicity in murine macrophages. In leishmaniasis cutanea experiment, mortality, weight loss, lesion size and burden parasite were measured. RESULTS: Extracts evaluated showed inhibitive effect on growing of promastigote form; however, active extracts caused a high toxicity. Ethanol and n-hexane extracts demonstrated specific antileishmanial activity. Ethanol and n-hexane extracts from Pluchea carolinensis (P. carolinensis) caused similar inhibition against amastigote form. The intraperitoneal administration of the ethanol extract of P. carolinensis at 100 mg/kg prevented lesion development compared with control groups. CONCLUSIONS: The antileishmanial experiment suggests that ethanol extracts from P. carolinensis is the most promising. Further studies are still needed to evaluate the potential of this plant as a source of new antileishmanial agents.
Assuntos
Asteraceae/química , Leishmania/efeitos dos fármacos , Fitoterapia , Extratos Vegetais/farmacologia , Folhas de Planta/química , Animais , Modelos Animais de Doenças , Etanol , Feminino , Hexanos , Concentração Inibidora 50 , Camundongos , Camundongos Endogâmicos BALB C , Solventes , Resultado do TratamentoRESUMO
In countries where leishmaniasis is endemic, there are not very many treatment alternatives and most options have problems associated with their use. Plants and their natural products constitute good sources of interesting lead compounds that could be potentially active against Leishmania. Chenopodium ambrosioides is a plant that is widely used in popular medicine and its antiparasitic effects have been documented, including the antileishmanial potentialities of Chenopodium oil. The objective of this study was to determine the chemical composition, in-vitro cytotoxicity and antileishmanial activity of essential oils extracted from C. ambrosioides, which received different treatments prior to extraction. The chemical characterization by GC-MS of the three essential oil samples showed similar composition and the major components were alpha-terpinene (17.0-20.7%), p-cymene (20.2-21.1%) and ascaridole (30.5-47.1%). The essential oils exhibited similar antileishmanial activities against intracellular amastigote form, with IC50 values between 4.7 and 12.4 microg/mL. However, a lower cytotoxicity was displayed by the essential oil extracted from fresh green vegetable material, which was statistically different (P < 0.05) from the other samples. This study demonstrated that the prior treatment of plant material did not interfere with the antiparasitic activity of essential oils from C. ambrosioides but did change their cytotoxicity, which should be taken into account in further studies.
Assuntos
Antiprotozoários/farmacologia , Chenopodium ambrosioides/química , Leishmania mexicana/efeitos dos fármacos , Óleos Voláteis/análise , Óleos Voláteis/farmacologia , Animais , Feminino , Macrófagos/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos BALB CRESUMO
BACKGROUND: The aromatic herb Chenopodium ambrosioides is widely known for its antiparasitic activity. The aim of this study was to investigate the antileishmanial effect of Chenopodium oil administered by the oral route at different doses and to compare its action to conventional, clinically used drugs. MATERIALS AND METHODS: BALB/c mice were infected with Leishmania amazonensis and treated with 30, 60, 90, 120, and 150 mg/kg of the essential oil for 15 days. A second experiment was performed to compare the antileishmanial effect of Chenopodium oil with glucantime (28 mg/kg), amphotericin B (1 mg/kg), and pentamidine (4 mg/kg), which were administered daily over 15 days by the intraperitoneal route. RESULTS: Statistically significant differences were observed between BALB/c mice treated with all the doses of the product compared with untreated animals and the mice treated with the vehicle. A dose of 150 mg/kg was the most effective and no macroscopic toxic effects were observed. The size of lesion showed a linear correlation at each point (R > 0.8322), with a 50% effective concentration of 51.4 mg/kg. At 150 mg/kg, the essential oil showed better activity compared with animals treated with glucantime, amphotericin B, and pentamidine. CONCLUSION: C. ambrosioides caused a promising therapeutic effect against cutaneous leishmaniasis caused by L. amazonensis, which could be explored to develop a new alternative treatment for cutaneous leishmaniasis.
Assuntos
Antiprotozoários/farmacologia , Antiprotozoários/uso terapêutico , Chenopodium ambrosioides/química , Leishmania/efeitos dos fármacos , Leishmaniose Cutânea/tratamento farmacológico , Óleos Voláteis/farmacologia , Óleos Voláteis/uso terapêutico , Animais , Feminino , Camundongos , Camundongos Endogâmicos BALB C , Distribuição Aleatória , Resultado do TratamentoRESUMO
Introducción: la leishmaniasis es una enfermedad causada por varias especies del género Leishmania, de la cual se han incrementado los reportes en los últimos años. Diversos factores genéticos, inmunológicos y asociados al parásito determinan el establecimiento de la infección y la ocurrencia de enfermedad, que se presenta en formas clínicas muy diversas, lo cual condiciona, entre otros elementos, el método diagnóstico que se aplica. Métodos: se hizo una revisión de la literatura básica y actualizada sobre aspectos generales de la leishmaniasis: la enfermedad y su situación epidemiológica, el ciclo de vida y la transmisión, vectores, presentaciones clínicas y diagnóstico; este último acápite contiene información sobre los principales métodos que se utilizan en el presente. Se puntualizó en la forma en que se apoya el diagnóstico desde el grupo de Leishmania del Instituto de Medicina Tropical "Pedro Kourí", que constituye el centro nacional de referencia para esta enfermedad tropical. Resultados: se muestra información actualizada sobre los temas escogidos, con un enfoque orientador y práctico, dirigido al personal de salud que debe enfrentar casos con sospecha de leishmaniasis. Se muestran tablas y gráficos que resumen de manera organizada aspectos relevantes, así como el algoritmo de trabajo que se sigue en el instituto con relación al diagnóstico de esta enfermedad, con énfasis especial en las aplicaciones al diagnóstico molecular que ha realizado el grupo de trabajo. Conclusiones: debido a que la leishmaniasis permanece aún sin control, el diagnóstico oportuno continúa siendo una necesidad. Todos los métodos aportan información de utilidad para la toma de decisiones en el tratamiento clínico, la imposición del tratamiento y el enfoque epidemiológico de esta parasitosis. Entre ellos, proponemos un algoritmo de trabajo en nuestro laboratorio, con el empleo de los métodos que resultan más útiles de acuerdo a nuestras condiciones y experiencias.
Introduction: leishmaniasis is a disease caused by several species from Leishmania genus, which has been increasingly reported in the last few years. Several genetic, immunological factors and others related to this parasite have been associated to the outcome of the infection, and the occurrence of illness in varied clinical forms. All the aforementioned has an impact on the diagnostic method that should be used. Methods: a basic and recent literature review was made, mainly focused on general aspects of leishmaniasis as epidemiological situation of disease, life cycle and transmission, vectors, clinical presentation and diagnosis; the latter shows information about the main methods used at present. The procedure followed by the Leishmania group at "Pedro Kourí" Institute of Tropical Medicine to support the diagnostic activities was presented as well. Results: updated practical information about the chosen topics was presented, with a practical guiding approach aimed at the health personnel that must face suspected leishmaniasis cases. Tables and figures summarized relevant aspects in an organized form, as well as the working algorithm of Institute concerning diagnosis was presented. The application of molecular diagnosis by this working team was particularly underlined. Conclusions: as leishmaniasis is still out of control, opportune diagnosis remains a must. All the methods provide useful information for taking decisions on clinical management, treatment and epidemiology of this parasitosis; hence, a working algorithm was submitted in our lab based on the most useful methods under our present conditions and experience.
Assuntos
Humanos , DNA de Protozoário/análise , Leishmania/genética , Leishmaniose/diagnóstico , Leishmaniose/parasitologia , MicroscopiaRESUMO
Leishmaniasis is one of the most important parasitic infections, but current treatments are unsatisfactory due to their toxicity, cost and resistance. Therefore, the development of new antileishmanial compounds is imperative. Many people who live in endemic areas use plants as an alternative to treat the disease. In this paper, we characterised the essential oil from Piper auritum, evaluated its cytotoxicity and determined its antileishmanial activity. The chromatogram obtained by gas chromatography revealed 60 peaks and we found that safrole was the most abundant compound, composing 87 percent of the oil. The oil was active against the promastigotes of Leishmania major, Leishmania mexicana, Leishmania braziliensis and Leishmania donovani with a favourable selectivity index against peritoneal macrophages from BALB/c mice. The Piper-oil inhibited the growing of intracellular amastigotes of L. donovani with an IC50 value of 22.3 ± 1.8 ìg/mL. This study demonstrates the usefulness of the essential oils as a promising alternative to treat leishmaniasis.