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1.
Am J Physiol Heart Circ Physiol ; 323(6): H1108-H1117, 2022 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-36269650

RESUMO

Changes in vascular structure contribute to vascular events and loss of brain health. We examined changes in cerebral arterioles at the onset of hypertension and the hypothesis that alterations during hypertension would recover with the return of mean arterial pressure (MAP) to normal. MAP was measured with radiotelemetry in awake male C57BL/6J mice at baseline and during infusion of vehicle or angiotensin II (ANG II, 1.4 mg/kg/day using osmotic pumps) for 28 days, followed by a 28-day recovery. With ANG II treatment, MAP increased through day 28. On day 30, MAP began to recover, reaching levels not different from vehicle on day 37. We measured intravascular pressure, diameter, wall thickness (WT), wall:lumen ratio (W:L), cross-sectional area (CSA), and slope of the tangential elastic modulus (ET) in maximally dilated arterioles. Variables were similar in both groups at day 1, with no significant change with vehicle treatment. With ANG II treatment, CSA, WT, and W:L increased on days 7-28. Internal and external diameter was reduced at 14 and 28 days. ET versus wall stress was reduced on days 7-28. During recovery, the diameter remained at days 14 and 28 values, whereas other variables returned partly or completely to normal. Thus, CSA, WT, W:L, and ET versus wall stress changed rapidly during hypertension and recovered with MAP. In contrast, inward remodeling developed slowly and did not recover. This lack of recovery has mechanistic implications for the long-term impact of hypertension on vascular determinants of brain health.NEW & NOTEWORTHY Changes in vascular structure contribute to vascular events and loss of brain health. We examined the inherent structural plasticity of cerebral arterioles during and after a period of hypertension. Arteriolar wall thickness, diameter, wall-to-lumen ratio, and biological stiffness changed rapidly during hypertension and recovered with blood pressure. In contrast, inward remodeling developed slowly and did not recover. This lack of recovery of arteriolar diameter has implications for the long-term impact of hypertension on vascular determinants of brain health.


Assuntos
Pressão Arterial , Hipertensão , Animais , Camundongos , Masculino , Camundongos Endogâmicos C57BL , Pressão Sanguínea , Arteríolas , Angiotensina II/farmacologia
2.
Hypertension ; 51(4): 867-71, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18285614

RESUMO

The transcription factor PPARgamma is expressed in endothelium and vascular muscle where it may exert antiinflammatory and antioxidant effects. We tested the hypothesis that PPARgamma plays a protective role in the vasculature by examining vascular structure and function in heterozygous knockin mice expressing the P465L dominant negative mutation in PPARgamma (L/+). In L/+ aorta, responses to the endothelium-dependent agonist acetylcholine (ACh) were not affected, but there was an increase in contraction to serotonin, PGF(2alpha), and endothelin-1. In cerebral blood vessels both in vitro and in vivo, ACh produced dilation that was markedly impaired in L/+ mice. Superoxide levels were elevated in cerebral arterioles from L/+ mice and responses to ACh were restored to normal with a scavenger of superoxide. Diameter of maximally dilated cerebral arterioles was less, whereas wall thickness and cross-sectional area was greater in L/+ mice, indicating cerebral arterioles underwent hypertrophy and remodeling. Thus, interference with PPARgamma signaling produces endothelial dysfunction via a mechanism involving oxidative stress and causes vascular hypertrophy and inward remodeling. These findings indicate that PPARgamma has vascular effects which are particularly profound in the cerebral circulation and provide genetic evidence that PPARgamma plays a critical role in protecting blood vessels.


Assuntos
Circulação Cerebrovascular/fisiologia , Hipertensão/fisiopatologia , PPAR gama/genética , PPAR gama/metabolismo , Transdução de Sinais/fisiologia , Acetilcolina/farmacologia , Animais , Aorta/efeitos dos fármacos , Aorta/fisiologia , Arteríolas/patologia , Arteríolas/fisiopatologia , Dinoprosta/farmacologia , Endotelina-1/farmacologia , Feminino , Perfilação da Expressão Gênica , Genes Dominantes , Hipertensão/patologia , Hipertrofia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Mutantes , Serotonina/farmacologia , Serotoninérgicos/farmacologia , Transdução de Sinais/efeitos dos fármacos , Vasodilatadores/farmacologia
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