Ca2+ and phospholipid-dependent protein kinase activity in rat cerebral hemispheres.

Ca2+/phospholipid-dependent protein kinase (PKC) activity was found to be asymmetrically distributed between the two cerebral hemispheres of rat brain, whereas basal protein phosphorylation was not lateralized. The left cerebral hemisphere (LCH) displayed about 50% more PKC activity in synaptosomal fractions than the right cerebral hemisphere (RCH). Polyacrylamide gel electrophoresis, autoradiography and quantitation of radioactivity in individual protein bands showed that the phosphate acceptors with major interhemispheric differences were proteins of more than 50 kDa. Cerebral lateralization was also apparent in the pattern of PKC inhibition mediated by phospholipid-interacting drugs: chlorpromazine and polymyxin B depressed activity more profoundly in LCH. A covalent protein modification usually associated with neurotransmitter receptor activation is thus unevenly distributed in rodent brain.

Endogenous asymmetry of rat brain lipids and dominance of the right cerebral hemisphere in free fatty acid response to electroconvulsive shock.

An asymmetric distribution of free fatty acids (FFA) is shown to occur between right and left cerebral hemispheres (RCH, LCH) of the rat. The RCH contains 35% less FFA than the LCH, the difference being mainly accounted for by saturated and monoenoic fatty acids. Acute and chronic electroconvulsive shock (ECS) affects the distribution and apparent rate of fatty acid production differently in each hemisphere. Taking into consideration the basal content of each hemisphere, RCH produces significantly higher amounts of FFA during the acute tonic phase of the convulsion evoked by a single ECS. The largest increases correspond to arachidonic and stearic acids (1800% and 420% in RCH, 1200% and 330% in LCH, respectively). The hemispheric sidedness is evened out after successive ECSs. The removal of the released fatty acids is also faster in the RCH, as suggested by its lower FFA levels 5 min after a single shock (the acute condition) or after the last of a series of 5 daily shocks (the chronic condition). The endogenous FFA content and composition is altered by chronic ECSs. Thus, 24 h after the last of a series of 4 daily ECSs, total FFAs remain about 40% higher than in the controls for both hemispheres. Arachidonic acid increase amounts to 150%, doubling its percentage contribution to the FFA pool. The lower endogenous FFA content in RCH, its higher responsiveness to ECS, and its ability to more rapidly recover the pre-convulsive levels, suggest that the deacylation and reacylation of complex lipids are more active in this hemisphere.(ABSTRACT TRUNCATED AT 250 WORDS)

Asymmetry of diacylglycerol metabolism in rat cerebral hemispheres.

Diacylglycerols (DGs) were found to be asymmetrically distributed between the two cerebral hemispheres of rat brain. The left cerebral hemisphere (LCH) contained 100% more DG than the right cerebral hemisphere (RCH). The lateralization was enhanced in animals subjected to depolarization induced by a single electroconvulsive shock (ECS). During the acute phase of the convulsion, the DG pool increased in both hemispheres, with the LCH attaining a concentration 180% higher than the RCH. Stearate and arachidonate were the principal DG-acyl groups accumulated in the RCH, whereas in the LCH stearate and palmitate were mainly involved. After the last of a series of five shocks (one per day) the lateralization of the "DG response" was less accentuated during the acute phase of the ECS. Whereas DG release was drastically reduced in the LCH, in the RCH it was minimally affected. The DG sidedness after five shocks was nevertheless maintained at the level of arachidonate-containing DGs, which showed a higher accumulation in the LCH than in the RCH. The kinetics of DG removal showed a rapid phase during the first minute following a single or five ECSs. Total DG levels returned to basal values in the RCH, whereas in the LCH they remained slightly increased with respect to the initial levels 1 min after the convulsive episode. Minimal changes occurred in the subsequent 4 min. Chronic ECS altered the endogenous DG content and composition. Thus, 24 h after the last of four ECSs, total levels of DGs diminished by 40% in the RCH, whereas they remained unchanged in the LCH.(ABSTRACT TRUNCATED AT 250 WORDS)