Distributions of waterborne pathogens in raw wastewater based on a 14-month, multi-site monitoring campaign.

The California State Water Resources Control Board is the first regulatory body in the United States to develop statewide regulations for direct potable reuse (DPR). To support this effort, a pathogen monitoring campaign was undertaken to develop and implement an optimized standard operating protocol to better characterize the concentration of human pathogens in raw wastewater. Methods to detect relevant viral and protozoan pathogens in raw wastewater were optimized and implemented during a 14-month monitoring campaign. Over 120 samples were collected from five wastewater treatment plants treating a quarter of California's population. Samples were analyzed for two protozoa (Cryptosporidium and Giardia) using microscopy methods, three enteric viruses (enterovirus, adenovirus, and norovirus) using culture and/or molecular methods, and male-specific coliphage using culture methods. The method recovery efficiency was measured in every protozoa sample and every other virus sample to confirm minimum recovery efficiencies were achieved and to correct the concentrations for pathogen losses during sample processing. The results from this study provide the industry with a large, high-quality dataset as demonstrated by the high degree of method sensitivity, method recovery, and QA/QC steps. Such high-quality data on pathogen concentrations in raw wastewater are critical for confirming the level of treatment needed to reduce pathogen concentrations down to acceptable levels for potable water in DPR projects.

Pressure monitoring and characterization of external sources of contamination at the site of the payment drinking water epidemiological studies.

The 1990s epidemiological studies by Payment and colleagues suggested that an increase in gastrointestinal illnesses observed in the population consuming tap water from a system meeting all water quality regulations might be associated with distribution system deficiencies. In the current study, the vulnerability of this distribution system to microbial intrusion was assessed by characterizing potential sources of contamination near pipelines and monitoring the frequency and magnitude of negative pressures. Bacterial indicators of fecal contamination were recovered more frequently in the water from flooded air-valve vaults than in the soil or water from pipe trenches. The level of fecal contamination in these various sources was more similar to levels from river water rather than wastewater. Because of its configuration, this distribution system is vulnerable to negative pressures when pressure values out of the treatment plant reach or drop below 172 kPa (25 psi), which occurred nine times during a monitoring period of 17 months. The results from this investigation suggest that this distribution system is vulnerable to contamination by intrusion. Comparison of the frequency of occurrence of negative pressure events and repair rates with data from other distribution systems suggests that the system studied by Payment and colleagues is not atypical.