Association between hepatic angiogenesis and serum adipokine profile in non-obese chronic hepatitis C patients.

It is unclear whether angiogenesis merely represents a homeostatic mechanism aimed at ensuring an adequate oxygen supply or one that exerts an additional pathogenic role leading to liver damage in chronic hepatitis. Chronic hepatitis C (CHC) patients present a proangiogenic profile of angiogenic markers. Adipokines not only regulate adipose tissue and glucose metabolism, but also influence inflammation, fibrogenic process and production of proangiogenic factors. On the basis of this evidence we aimed to assess the number of new blood vessels in lobules and portal tracts in the liver and evaluate the relationship between angiogenesis intensity and serum adipokine concentrations in CHC. Our study showed a positive association between serum vaspin and angiogenesis intensity in portal tracts and lobules in CHC patients (r = 0.41, p = 0.04; r = 0.46, p = 0.03; respectively). Serum visfatin was found to be negatively related to angiogenesis in portal tracts and lobules but only in females (r = -0.76, p = 0.03; r= -0.95, p < 0.001; respectively). In conclusion, the role of some adipokines in liver angiogenesis seems to be different in females than in males. Serum vaspin concentration seems to reflect intensity of liver angiogenesis in CHC. Further studies are necessary to better determine the role of adipokines in new blood vessel formation in CHC.

[Emergency and elective cesarean section and selected elements of immunological system of full-term neonates]. / Wskazania nagle lub elektywne do ciecia cesarskiego a wybrane elementy ukladu immunologicznego u noworodków urodzonych o czasie.

AIM OF STUDY: To define a relationship between emergency and elective indications for cesarean section and quantitative changes of percentage and quantity of T and B lymphocytes and their subpopulations in healthy full-term neonates. MATERIAL AND METHODS: The study included 43 healthy neonates born in the years 1998 - 2003 in the Department of Perinatology and Gynecology of Silesian Medical University in Zabrze, Taking into account operative delivery, a group of 43 full-term neonates born by cesarean section were examined, including 25 neonates with elective indications [Ibe] and 18 with emergency indications (Ibn). Immunological studies included evaluation of the percentage of T and B lymphocytes and of their subpopulations in umbilical blood. Our study applied a method of umbilical blood sampling with the following red blood cells lysis. RESULTS: The study showed that mean number of CD3+, CD23+, CD25+ lymphocytes and mean percentage and number of CD8+ lymphocytes in full-term neonates born by elective Cesarean was statistically significantly lower than in neonates born by emergency Cesarean section. No statistically significant differences were found in mean values of other parameters among the study groups. CONCLUSIONS: Cesarean section performed in a mother with emergency indications can favor an increase of mean quantity of T lymphocytes, cytotoxic - suppressor T lymphocytes, activated T lymphocytes, B CD23 lymphocytes in full-term neonates.

[Microcytomorphometric video-image detection of nuclear chromatin in ovarian cancer]. / Diagnostyka mikrocytomorfometryczna typu video-image chromatyny jadrowej w raku jajnika.

UNLABELLED: Technology of detection of tissue preparates precisious evaluates contents of nuclear chromatine, largeness and shape of cellular nucleus, indicators of mitosis, DNA index, ploidy, phase-S fraction and other parameters. Methods of detection of picture are: microcytomorphometry video-image (MCMM-VI), flow, double flow and activated by fluorescence. Diagnostic methods of malignant neoplasm of ovary are still nonspecific and not precise, that is a reason of unsatisfied results of treatment. OBJECTIVE: Evaluation of microcytomorphometric measurements of nuclear chromatine histopathologic tissue preparates (HP) of ovarian cancer and comparison to normal ovarian tissue. MATERIAL AND METHODS: Estimated 10 paraffin embedded tissue preparates of serous ovarian cancer, 4 preparates mucinous cancer and 2 cases of tumor Kruckenberg patients operated in Clinic of Perinatology and Gynaecology Silesian Medical Academy in Zabrze in period 2001-2002, MCMM-VI estimation based on computer aided analysis system: microscope Axioscop 20, camera tv JVCTK-C 1380, CarlZeiss KS Vision 400 rel.3.0 software. Following MCMM-VI parameters assessed: count of pathologic nucleus, diameter of nucleus, area, min/max diameter ratio, equivalent circle diameter (Dcircle), mean of brightness (mean D), integrated optical density (IOD = area x mean D), DNA index and 2.5 c exceeding rate percentage (2.5 c ER%). MCMM-VI performed on the 160 areas of 16 preparates of cancer and 100 areas of normal ovarian tissue. Statistical analysis was performed by used t-Student test. RESULTS: We obtained stastistically significant higher values parameters of nuclear chromatine, DI, 2.5 c ER of mucinous cancer and tumor Kruckenberg comparison to serous cancer. MCMM-VI parameters of chromatine malignant ovarian neoplasm were statistically significantly higher than normal ovarian tissue. CONCLUSION: Cytometric and karyometric parametres of nuclear chromatine estimated MCMM-VI are useful in the diagnostics and prognosis of ovarian cancer.

[Ovarian tumors in pregnancy]. / Guzy jajnika wspólistniejace z ciaza.

OBJECTIVE: The clinical characteristics of pregnancy complicated by ovarian tumors were investigated. MATERIAL AND METHODS: A review was performed of patients who were seen with ovarian tumors in pregnancy from January 1985 to August 2000. We included patients with simple or complex tumors > 5 cm that were seen on ultrasonographic evaluation. RESULTS: 23 patients of 21,506 deliveries were identified with ovarian tumors that met the criteria. 21 had operative intervention, whereas 2 were managed conservatively. Gestational ages at the time of surgery ranged from 16 to 24 weeks. No intraoperative et postoperative maternal or fetal complications occurred. In 2 cases adnexal masses seen on ultrasonography resolved spontaneously by 16 weeks. 19 ovarian tumors were benign. One patient had a stage III ovarian carcinoma and went to total hysterectomy, and other patient had a stage IA ovarian carcinoma and went to simple adnexectomy. CONCLUSIONS: Ovarian surgery in pregnancy for persistent masses is important to obtain a final histologic diagnosis. Optimal gestational age for the surgery is among 16 and 24 weeks.

[Rare case of a malignant brain neoplasm in an 18-years old pregnant woman]. / Rzadki przypadek nowotworu zlosliwego mózgu u 18-letniej ciezarnej.

Authors presented rare case of anaplastic ependymoma diagnosed in the first trimester of pregnancy 18-years of age patient. Main symptoms of increased intracranial pressure and cerebral edema were vomits, severe headache and apathy. In the sixteen week of pregnancy performed craniotomy and evacuated malignant tumor of region IV ventricle trunk of the brain. Follow during pregnancy made brachytherapy (LDR). Cesarean section performed in the 30th week of pregnancy. Then indispensable radical radiotherapy was continued.

[Prevention of intrapartum fetal hypoxia by using electrocardiography]. / Profilaktyka sródporodowego niedotlenienia z wykorzystaniem elektrokardiografii plodowej.

The biophysical diagnostic methods are an unusual element of evaluation of the fetus state in the time of pregnancy and delivery. Nowadays, the applied methods of fetus supervision during labor don't ensure the permanent analysis and they don't deliver diagnostic information about fetus hypoxia. The purpose of avoiding the risk of fetus injury and its hypoxia should be developed by technologies which allow making a diagnosis of fetus condition and to detection of intralabor hypoxia, So, especially all new methods are indicated, which make easier the diagnostic in a permanent mode and which allow for hypoxia expectation and they also allow to reduce the number of unjustified medical interference. One of these methods is fetus electrocardiography.

[Ovarian cancer during pregnancy--two case reports]. / Rak jajnika wspólistniejacy z ciaza--opis dwóch przypadków.

Authors presented two different cases of ovarian cancer during pregnancy. The first case in the stage IA had favourable course. We performed ovariectomy in the second trimester. At the term of delivery cesarean section with total hysterectomy and omentectomy with following chemotherapy (PC) were carried out. The second case (IIIC stage) had a poor course and diagnosis (Cystadenocarcinoma papillary GIII). The management comprised of total hysterectomy and omentectomy with following chemotherapy (PAC + Bleomycin + Mitomycin). Despite our treatment the development of cancer was observed. The patient died after 8 months since operation. Early diagnosis and lower stage of cancer give better prognosis, however, clinical management is still discussed.

[Herbich cervical pessary--method of therapy for cervical incompetence and prophylaxis of prematurity]. / Pessar szyjkowy Herbicha sposobem terapii niewydolnosci ciesniowo-szyjkowej i profilaktyki wczesniactwa.

Cervical incompetence is often complication in the II and III trimester of pregnancy and is a cause of 16-20% of premature deliveries. Treatment of incompetent cervix contains surgical methods ( cerclage and others) and conservative management as follow: tocolytics, hormones, bed rest and mechanical factors (pessary, rings, balloons etc). The aim of the study was evaluation of therapeutical efficacy Herbich cervical pessary in the cases of cervical incompetence I and II degree in II and III trimester of pregnancy. Study included 73 pregnant women with cervical incompetence diagnosed on the basis of clinical examination and measurements of cervical length during transvaginal ultrasonography (Voluson 730 Kretz, ATL HDI 1000). After diagnosis we inserted into vagina pessary size 1 or 2 around incompetent cervix. We divided examination group into primipara and multipara. In our research we obtained no statistically significant differences between both groups in the percentage of premature deliveries, the mode of delivery and prolong time of pregnancy. High percentage of prematurity (35.7%) was a cause of two fetal death (2.75%) delivered in the 25 week of pregnancy. Cervical pessary prolonged time of pregnancy about 12-17 weeks and for this reason almost all labors were after 33 weeks of pregnancy (89%). Cervical pessary of Herbich is a safe and effective method of treatment for cervical incompetence of I and II degree. This pessary decreases percentage of prematurity in the high risk group of pregnant women with incompetent cervix.