RESUMO
A polyethylene glycol (PEG) precipitation F(ab')2 anti-C3 ELISA for the detection of complement-fixing IgG circulating immune complexes (CIC) is described. For this assay, test sera were treated with 3.5% PEG and then measured with F(ab')2 anti-C3 ELISA. The lower detection limit was 4 micrograms/ml of heat aggregated human IgG (HAHG). Intra-assay coefficient of variation (CV) was 4.9-8.3%. High levels of CIC are found in the sera of patients with systemic lupus erythematosus (SLE), hepatitis B and stomach cancer.
Assuntos
Complexo Antígeno-Anticorpo/análise , Complemento C3/imunologia , Ensaio de Imunoadsorção Enzimática/métodos , Fragmentos Fab das Imunoglobulinas/imunologia , Polietilenoglicóis , Precipitação Química , Testes de Fixação de Complemento , Hepatite B/imunologia , Humanos , Lúpus Eritematoso Sistêmico/imunologia , Reprodutibilidade dos Testes , Neoplasias Gástricas/imunologiaRESUMO
A study on the relation between urinary cations and blood pressure was conducted in Wuhan, China. A total of 148 boys aged 7-8 years entered the study from November 23, 1984, to January 13, 1985. Seven consecutive 24-hour urine specimens were collected and blood pressure was measured on the seven successive days corresponding to urine collection. The multiple regression showed that none of the sodium, potassium, calcium, and magnesium cations was statistically significantly related to blood pressure. However, the ratio of sodium/calcium or potassium/calcium in the urine was positively correlated with systolic pressure in multivariate analysis. The results suggest that there may be a complex interaction between sodium and calcium in their effects on blood pressure, and genetic background might have some influences on the sodium/calcium (or potassium/calcium)-blood pressure relation in the 7-8-year-old boys in the study.