Aqueous Extracts of Pleurotus ostreatus and Hericium erinaceus Protect against Ultraviolet A-Induced Damage in Human Dermal Fibroblasts.

Pleurotus ostreatus (PO) and Hericium erinaceus (HE) have been traditionally used to treat various diseases, owing to their antioxidant, antimicrobial, neuroprotective, and antitumor effects. However, few studies have been reported on their antiaging effects. In this study, the antioxidant and antiaging activities of PO and HE aqueous extracts were investigated in ultraviolet A (UVA)-induced human dermal fibroblast cells (HDFs). The antioxidant properties of PO and HE aqueous extracts were measured by total polyphenol and ergothioneine content, and their antioxidant activity was analyzed with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical-scavenging assays. To demonstrate the antiaging effect of PO and HE aqueous extracts in UVA-induced HDFs, the secretion and mRNA expression of matrix metalloproteinase-1 (MMP-1), procollagen type I (PC1), and elastase were assessed by enzyme-linked immunosorbent assay (ELISA) and real-time PCR, respectively. The total polyphenol content in each extract was 13.6 and 11.7 mg gallic acid equivalents/g dry weight (DW), respectively, and the total ergothioneine content in each extract was 3.43 and 2.18 mg/g DW, respectively. The PO and HE extracts increased DPPH and ABTS radical-scavenging activity in a dose-dependent manner. In UVA-damaged HDFs, the extracts increased PC1 production but decreased MMP-1 production and elastase-1 activity. Furthermore, the mRNA levels of PC1, MMP-1, and elastase were recovered in the PO- and HE-treated UVA-irradiated HDFs compared to those in the irradiated control group. PO and HE aqueous extracts may be potentially used as a promising antiphotoaging agent.

Phytochemical profile and antioxidant activity of Dracocephalum moldavica L. seed extracts using different extraction methods.

Dracocephalum moldavica L. (DM) is used to treat cardiovascular diseases and dermatitis. However, the properties of DM seed and its extracts remain unclear. We analyzed the bioactive compounds and antioxidant activity of aqueous extract (AE), ethanolic extract (EE), and supercritical CO2 extracted oil (SC-oil) of DM seed. Alpha-linolenic acid was the most abundant fatty acid (51.4-61.6%) with an ω-3 to ω-6 ratio of 2.93-3.42 to 1. Stigmasterol and ß-sitosterol + fucosterol were the main components of DM seed extracts, and campesterol was detected only in SC-oil. The total tocopherol content, especially γ-tocopherol, was 60% higher in EE than in AE and SC-oil, whereas ß-carotene was detected only in SC-oil. The content of total phenols and flavonoids was the highest in EE. Rosmarinic acid, apigenin, and caffeic acid were detected only in EE. The radical scavenging activity of EE was the highest in EE. The results can help promote DM application.