Multifrequency Investigation of Single- and Double-Stranded DNA with Scalable Metamaterial-Based THz Biosensors.

Due to the occurrence of THz-excited vibrational modes in biomacromolecules, the THz frequency range has been identified as particularly suitable for developing and applying new bioanalytical methods. We present a scalable THz metamaterial-based biosensor being utilized for the multifrequency investigation of single- and double-stranded DNA (ssDNA and dsDNA) samples. It is demonstrated that the metamaterial resonance frequency shift by the DNA's presence depends on frequency. Our experiments with the scalable THz biosensors demonstrate a major change in the degree of the power function for dsDNA by 1.53 ± 0.06 and, in comparison, 0.34 ± 0.11 for ssDNA as a function of metamaterial resonance frequency. Thus, there is a significant advantage for dsDNA detection that can be used for increased sensitivity of biomolecular detection at higher frequencies. This work represents a first step for application-specific biosensors with potential advantages in sensitivity, specificity, and robustness.

Phase-locking of the beat signal of two distributed-feedback diode lasers to oscillators working in the MHz to THz range.

We present difference-frequency stabilization of free-running distributed-feedback (DFB) diode lasers, maintaining a stable phase-lock to a local oscillator (LO) signal. The technique has been applied to coherent hybrid THz imaging which employs a high-power electronic radiation source emitting at 0.62 THz and electro-optic detectors. The THz radiation of the narrow-band emitter is mixed with the difference frequency of the DFB diode laser pair. The resulting intermediate frequency is phase-locked to the LO signal from a radio-frequency generator using a fast laser-current control loop. The stabilization scheme can be adapted readily to a wide range of applications which require stabilized laser beat-notes.

Ultrasensitive THz biosensor for PCR-free cDNA detection based on frequency selective surfaces.

THz technologies are a powerful tool for label-free detection of biomolecules. However, significant reduction of the lower detection limit is required to apply THz-sensors in biomedical diagnosis. This paper reports an ultrasensitive THz-biosensor based on asymmetric double split ring resonators (aDSRR) for the direct label- and PCR-free detection of DNA at physiologically relevant concentrations. We introduce selective functionalization and localized electric field concentration to enhance aDSRR sensitivity and specificity. The sensor characteristics are demonstrated using the human tumor marker MIA in cDNA samples produced from total RNA without PCR-amplification. Measurements of DNA samples with concentrations as low as 1.55 × 10-12 mol/l are presented.

Reconfigurable THz Plasmonic Antenna Based on Few-Layer Graphene with High Radiation Efficiency.

Graphene plasmonic antennas possess two significant features that render them appealing for short-range wireless communications, notably, inherent tunability and miniaturization due to the unique frequency dispersion of graphene and its support for surface plasmon waves in the terahertz band. In this letter, dipole-like antennas using few-layer graphene are proposed to achieve a better trade-off between miniaturization and radiation efficiency than current monolayer graphene antennas. The characteristics of few-layer graphene antennas are evaluated and then compared with those of antennas based on monolayer graphene and graphene stacks, which could also provide such improvements. To this end, first, the propagation properties of one-dimensional and two-dimensional plasmonic waveguides based on the aforementioned graphene structures are obtained by transfer matrix theory and finite-element simulation, respectively. Second, the antennas are investigated as three-dimensional structures using a full-wave solver. Results show that the highest radiation efficiency among the compared designs is achieved with the few-layer graphene, while the highest miniaturization is obtained with the even mode of the graphene stack antenna.

High Photocurrent in Gated Graphene-Silicon Hybrid Photodiodes.

Graphene/silicon (G/Si) heterojunction based devices have been demonstrated as high responsivity photodetectors that are potentially compatible with semiconductor technology. Such G/Si Schottky junction diodes are typically in parallel with gated G/silicon dioxide (SiO2)/Si areas, where the graphene is contacted. Here, we utilize scanning photocurrent measurements to investigate the spatial distribution and explain the physical origin of photocurrent generation in these devices. We observe distinctly higher photocurrents underneath the isolating region of graphene on SiO2 adjacent to the Schottky junction of G/Si. A certain threshold voltage (VT) is required before this can be observed, and its origins are similar to that of the threshold voltage in metal oxide semiconductor field effect transistors. A physical model serves to explain the large photocurrents underneath SiO2 by the formation of an inversion layer in Si. Our findings contribute to a basic understanding of graphene/semiconductor hybrid devices which, in turn, can help in designing efficient optoelectronic devices and systems based on such 2D/3D heterojunctions.

Label-free THz sensing of genetic sequences: towards 'THz biochips'.

THz-wave-based approaches for the label-free characterization of genetic material are described. Time-resolved THz spectroscopic analysis of genetic sequences (polynucleotides) demonstrate a distinct complex refractive index in the THz frequency range as a function of the binding state of the analysed DNA sequences. By monitoring THz signals, one can thus infer the binding state of oligo- and polynucleotides, enabling the label-free determination of the genetic composition of target polynucleotides by sensing their binding to known probe molecules. Here we review integrated THz sensing array developments exhibiting high sensitivity and single-base mutation detection capabilities. Recent achievements using functionalized biosensing arrays of high-Q resonators are illustrated.