Insulin-like growth factor binding protein 2 null mice (Igfbp2-/-) are protected against trabecular bone loss after vertical sleeve gastrectomy.

BACKGROUND: Bariatric surgery has been shown to result in weight loss, improved hemoglobin A1C, and decreased mortality but can also lead to bone loss and increased fracture rates. Serum IGFBP-2 is elevated in patients after bariatric surgery and although it may lead to improved blood glucose, may also drive bone resorption, and inhibit IGF-I action. This study tested the hypothesis that Igfbp2-/- mice were acutely protected from bone loss after vertical sleeve gastrectomy (VSG). METHODS: Thirty-four mice, 17 Igfbp2-/- and 17 + / + underwent a hand-sewn VSG or sham surgery, at 16 weeks of age. Mice were harvested at 20 weeks of age. DXA was measured for body composition, areal bone mineral density (aBMD), areal bone mineral content (aBMC), femoral bone mineral density (fBMD), and femoral bone mineral content (fBMC) at 15, 18, and 20 weeks of age. Micro-computed tomography and serum ELISA assays were measured and analyzed at 20 weeks of age. RESULTS: Both Igfbp2-/- and + / + mice lost significant weight (P = 0.0251, P = 0.0003, respectively) and total fat mass (P = 0.0082, P = 0.0004, respectively) at 4 weeks after VSG. Igfbp2+/+ mice lost significant aBMD, fBMD, fBMC, trabecular BMD, trabecular BV/TV and cortical tissue mineral density (P = 0.0150, P = 0.0313, P = 0.0190, P = 0.0072, and 0.0320 respectively). The Igfbp2-/- mice did not show significant bone loss in these parameters nor in trabecular BV/TV. Both Igfbp2-/- and + / + mice had less cortical bone area (P = 0.0181, P = < .00001), cortical area over total area (P = 0.0085, P = 0.0007), and cortical thickness (P = 0.0050, P = < 0.0001), respectively. Igfbp2+/+ mice demonstrated significantly lower polar, minimum, and maximum moments of inertia (P = 0.0031, P = 0.0239, and P = 0.0037, respectively). Igfbp2+/+ had significantly higher levels of IGFBP-2 at 2 weeks postoperatively after VSG (P = 0.035), and elevated levels of CTx and P1NP (P = 0.0127, P = 0.0058, respectively). CONCLUSIONS: Igfbp2-/- mice were protected against trabecular bone loss and had attenuated cortical bone loss 4 weeks after VSG.

Prolonged standing increases lower limb arterial stiffness.

PURPOSE: Standing workstations have recently been promoted as a healthy alternative to sitting. However, it is unknown how prolonged standing affects arterial stiffness, a prognostic indicator of cardiovascular health. The purpose of this study was twofold: to observe changes in arterial stiffness, as assessed by pulse wave velocity (PWV), with a 2-h bout of standing, and to determine if short, intermittent walking bouts provide a comparative advantage to standing alone. METHODS: Nineteen adults had arterial stiffness assessed by pulse wave velocity. Central (CPWV), upper peripheral (UPWV), and lower peripheral (LPWV) PWV were assessed before (supine), during standing (min 10, 60, and 120), and after (supine) the 2-h standing bout. In one trial, the participants stood at a standing desk immobile for 2 h. In the other trial, participants performed 5-min walking breaks after every 25 min of standing. RESULTS: After 2-h of standing, supine (85.8 ± 90.1 cm/s) and standing (303.4 ± 390.2 cm/s), LPWV increased independent of trial (i.e., main effect of time; p < 0.001). Walking breaks during 2 h of standing did not significantly attenuate these changes. In addition, standing CPWV decreased over time (- 38.5 ± 61.5 cm/s; p = 0.04). Yet, UPWV, standing or supine, did not change over the course of standing (p > 0.05). CONCLUSIONS: These findings indicate that prolonged standing increases the measures of arterial stiffness and there is no evidence that walk breaks attenuate this response.

Traumatic Simultaneous Bilateral Knee Dislocation: A Case Report.

Bilateral knee dislocation is an extremely rare and devastating injury to the knee joint. This injury is a true emergency as concomitant injuries may threaten life and limb. Here, we report the case of a 26-year-old male patient who sustained bilateral knee dislocation due to a high-velocity motor vehicle accident. In this case, the patient suffered a feared complication associated with knee dislocation, popliteal artery disruption with peroneal nerve damage. Abdominal and skeletal injuries were also discovered and treated appropriately by a multidisciplinary team. A staged surgical approach to ligament reconstruction was used without any postoperative complication. Postoperatively, the patient successfully returned to his preoperative activity level.

Management of bilateral osteochondritis dissecans of the trochlea in a skeletally immature patient.

Osteochondritis dissecans (OCD) lesions are injuries that occur more commonly in the skeletally immature population. In most cases, the aetiology is not well understood, but fortunately, many OCD lesions may heal on their own over time, particularly in skeletally immature patients with open physes. Conversely, if the lesion is considered unstable, surgical intervention may be required. This case demonstrates an especially rare presentation of bilateral OCD lesions within the lateral femoral trochlear facet. The lesions became symptomatic approximately 1 year apart without a specific injury. Non-operative treatment was not recommended in either case due to the size and instability of each lesion. The surgical treatment used an augmented microfracture technique. At 12 and 23 months after surgery, both knees remain asymptomatic and the patient has returned to their desired activities.

Pediatric Anterior Cruciate Ligament Reconstruction: A Reason Not to Wait Until Skeletal Maturity.

Anterior cruciate ligament (ACL) tears within the skeletally immature population give rise to controversy regarding the timing of treatment decisions due to the concern of iatrogenic damage to the open physis. Physis disruption from the required intraoperative graft tunnel drilling can lead to growth disturbance, thus ligament reconstruction is not without risk. Nonoperative management carries the risk of future damage to the menisci and cartilage as an ACL-deficient knee can be unstable. This particular case of a skeletally immature 10-year old male demonstrates an initial course of nonoperative treatment which ultimately resulted in previously undiagnosed meniscal damage. Failure of the nonoperative treatment was followed by a successful ACL reconstruction and meniscal repair surgery utilizing a partial physeal sparing technique. The patient successfully returned to his preoperative activity level without any graft disruption, postoperative indications of meniscus pathology, or abnormal growth deformities. This case report adds to the current literature reporting successful and safe ACL reconstructions in a skeletally immature patient.