RESUMO
The euryarchaeal transcriptional repressor NrpR regulates a variety of nitrogen assimilation genes by 2-oxoglutarate-reversible binding to conserved palindromic operators. The number and positioning of these operators varies among promoter regions of regulated genes, suggesting NrpR can bind in different patterns. Particularly intriguing is the contrast between the nif and glnK(1) promoter regions of Methanococcus maripaludis, where two operators are present but with different configurations. Here we study NrpR binding and regulation at the glnK(1) promoter, where the two operator sequences overlap and occur on opposite faces of the double helix. We find that both operators function in binding, with a dimer of NrpR binding simultaneously to each overlapping operator. We show in vivo that the first operator plays a primary role in regulation and the second operator plays an enhancing role. This is the first demonstration of overlapping operators functioning in Archaea.
Assuntos
Proteínas Arqueais/genética , Regulação da Expressão Gênica em Archaea , Mathanococcus/genética , Óperon , Proteínas Repressoras/metabolismo , Proteínas Arqueais/metabolismo , Sítios de Ligação , Ácidos Cetoglutáricos/metabolismo , Mathanococcus/metabolismo , Nitrogênio/metabolismo , Regiões Operadoras GenéticasRESUMO
To study global regulation in the methanogenic archaeon Methanococcus maripaludis, we devised a system for steady-state growth in chemostats. New Brunswick Bioflo 110 bioreactors were equipped with controlled delivery of hydrogen, nitrogen, carbon dioxide, hydrogen sulfide, and anaerobic medium. We determined conditions and media compositions for growth with three different limiting nutrients, hydrogen, phosphate, and leucine. To investigate leucine limitation we constructed and characterized a mutant in the leuA gene for 2-isopropylmalate synthase, demonstrating for the first time the function of this gene in the Archaea. Steady state specific growth rates in these studies ranged from 0.042 to 0.24 h(-1). Plots of culture density vs. growth rate for each condition showed the behavior predicted by growth modeling. The results show that growth behavior is normal and reproducible and validate the use of the chemostat system for metabolic and global regulation studies in M. maripaludis.
Assuntos
Mathanococcus/crescimento & desenvolvimento , 2-Isopropilmalato Sintase/genética , 2-Isopropilmalato Sintase/metabolismo , Anaerobiose , Biomassa , Reatores Biológicos , Meios de Cultura/química , Deleção de Genes , Genes Arqueais , Hidrogênio/metabolismo , Leucina/biossíntese , Leucina/metabolismo , Mathanococcus/genética , Mathanococcus/fisiologia , Mutação , Fosfatos/metabolismo , Fatores de TempoRESUMO
The use of molecular hydrogen as electron donor for energy generation is a defining characteristic of the hydrogenotrophic methanogens, an ancient group that dominates the phylum Euryarchaeota. We present here a global study of changes in mRNA abundance in response to hydrogen availability for a hydrogenotrophic methanogen. Cells of Methanococcus maripaludis were grown by using continuous culture to deconvolute the effects of hydrogen limitation and growth rate, and microarray analyses were conducted. Hydrogen limitation markedly increased mRNA levels for genes encoding enzymes of the methanogenic pathway that reduce or oxidize the electron-carrying deazaflavin, coenzyme F(420). F(420)-dependent redox functions in energy-generating metabolism are characteristic of the methanogenic Archaea, and the results show that their regulation is distinct from other redox processes in the cell. Rapid growth increased mRNA levels of the gene for an unusual hydrogenase, the hydrogen-dependent methylenetetrahydromethanopterin dehydrogenase.
Assuntos
Regulação da Expressão Gênica em Archaea/genética , Hidrogênio/farmacologia , Metano/metabolismo , Mathanococcus/genética , Mathanococcus/metabolismo , Proteínas Arqueais/genética , Proteínas Arqueais/metabolismo , Proliferação de Células , Mathanococcus/citologia , Mathanococcus/efeitos dos fármacos , Oxirredutases/metabolismo , RNA Mensageiro/genéticaRESUMO
Methanococcus maripaludis is a mesophilic species of Archaea capable of producing methane from two substrates: hydrogen plus carbon dioxide and formate. To study the latter, we identified the formate dehydrogenase genes of M. maripaludis and found that the genome contains two gene clusters important for formate utilization. Phylogenetic analysis suggested that the two formate dehydrogenase gene sets arose from duplication events within the methanococcal lineage. The first gene cluster encodes homologs of formate dehydrogenase alpha (FdhA) and beta (FdhB) subunits and a putative formate transporter (FdhC) as well as a carbonic anhydrase analog. The second gene cluster encodes only FdhA and FdhB homologs. Mutants lacking either fdhA gene exhibited a partial growth defect on formate, whereas a double mutant was completely unable to grow on formate as a sole methanogenic substrate. Investigation of fdh gene expression revealed that transcription of both gene clusters is controlled by the presence of H(2) and not by the presence of formate.