Zoning of some physicochemical parameters in the sediments of Meighan wetland in Iran: response to urbanization, industrial, and agricultural activities.

Meighan wetland is located at 8 km far away from Arak city with a population of about 600,000 citizens in the center of Iran. Several agricultural activities and industries such as metal, chemical, and mineral, as well as industrial towns, exist around the desired wetland. This research was conducted to measure the sources of chemical contaminants entering the wetland through natural and artificial waterways, to explore the trend of changes in the contaminants, and to prepare the wetland contamination zone map followed by source detection of these contaminants. Sediment samples were taken during 2019-2020 from a depth of 0-30 cm from 87 points in the input waterways. The results indicated that the mean total concentrations of cadmium, nickel, lead, zinc, copper, and aluminum in sediments were estimated as 6.7, 93.4, 14.1, 276.4, 34.3, and 22,742.7 ppm, respectively. The concentrations of nitrate and phosphate in the sediments were given as 18.6 and 1.8 ppm, respectively. The mean comparison indicated that the most significant level of nickel and lead belonged to the input waterways of industrial and urban regions; the maximum cadmium content was seen in the input waterways from the agricultural lands; and the highest level of zinc and aluminum was recorded in the waterways of agricultural-industrial urban regions. There was a significant relationship between the results of classic statistics and zoning found in GIS. Overall, chemical pollutants with the origin of input wastewater from the treatment plant of wastewater and the input waterways from the industrial and urban regions have had the largest share of contamination for Meighan wetland.

Immunomodulatory properties of quercetin-3-O-α-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus.

BACKGROUND: Influenza infection is a major public health threat. The role of influenza A virus-induced inflammatory response in severe cases of this disease is widely recognized. Drug resistance and side effects of chemical treatments have been observed, resulting in increased interest in alternative use of herbal medications for prophylaxis against this infection. The South African medicinal plant, Rapanea melanophloeos (RM) (L.) Mez of the family Myrsinaceae was selected owing to its traditional use for the treatment of several diseases such as respiratory ailments and also previous preliminary studies of anti-influenza activity of its methanolic extract. The aim of this study was to investigate the immunomodulatory properties of a glycoside flavone isolated from RM against influenza A virus. METHODS: The non-cytotoxic concentration of the quercetin-3-O-α-L-rhamnopyranoside (Q3R) was determined by MTT assay and tested for activity against influenza A virus (IAV) in simultaneous, pre-penetration and post-penetration combination treatments over 1 h incubation on MDCK cells. The virus titer and viral load targeting NP and M2 viral genes were determined using HA and qPCR, respectively. TNF-α and IL-27 as pro- and anti-inflammatory cytokines were measured at RNA and protein levels by qPCR and ELISA, respectively. RESULTS: Quercetin-3-O-α-L-rhamnopyranoside at 150 µg/ml decreased the viral titer by 6 logs (p < 0.01) in the simultaneous procedure. The NP and M2 genes copy numbers as viral target genes, calculated based on the Ct values and standard formula, significantly decreased in simultaneous treatment (p < 0.01). The expression of cytokines was also considerably affected by the compound treatment. CONCLUSIONS: This is the first report of quercetin-3-O-α-L-rhamnopyranoside from RM and its immunomodulatory properties against influenza A virus. Further research will focus on detecting the specific mechanism of virus-host interactions.