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1.
Cell Commun Signal ; 22(1): 291, 2024 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-38802835

RESUMO

A promising new therapy option for acute kidney injury (AKI) is mesenchymal stem cells (MSCs). However, there are several limitations to the use of MSCs, such as low rates of survival, limited homing capacity, and unclear differentiation. In search of better therapeutic strategies, we explored all-trans retinoic acid (ATRA) pretreatment of MSCs to observe whether it could improve the therapeutic efficacy of AKI. We established a renal ischemia/reperfusion injury model and treated mice with ATRA-pretreated MSCs via tail vein injection. We found that AKI mice treated with ATRA-MSCs significantly improved renal function compared with DMSO-MSCs treatment. RNA sequencing screened that hyaluronic acid (HA) production from MSCs promoted by ATRA. Further validation by chromatin immunoprecipitation experiments verified that retinoic acid receptor RARα/RXRγ was a potential transcription factor for hyaluronic acid synthase 2. Additionally, an in vitro hypoxia/reoxygenation model was established using human proximal tubular epithelial cells (HK-2). After co-culturing HK-2 cells with ATRA-pretreated MSCs, we observed that HA binds to cluster determinant 44 (CD44) and activates the PI3K/AKT pathway, which enhances the anti-inflammatory, anti-apoptotic, and proliferative repair effects of MSCs in AKI. Inhibition of the HA/CD44 axis effectively reverses the renal repair effect of ATRA-pretreated MSCs. Taken together, our study suggests that ATRA pretreatment promotes HA production by MSCs and activates the PI3K/AKT pathway in renal tubular epithelial cells, thereby enhancing the efficacy of MSCs against AKI.


Assuntos
Injúria Renal Aguda , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais , Tretinoína , Injúria Renal Aguda/terapia , Injúria Renal Aguda/patologia , Injúria Renal Aguda/metabolismo , Injúria Renal Aguda/tratamento farmacológico , Animais , Células-Tronco Mesenquimais/metabolismo , Células-Tronco Mesenquimais/efeitos dos fármacos , Células-Tronco Mesenquimais/citologia , Tretinoína/farmacologia , Tretinoína/uso terapêutico , Humanos , Camundongos , Masculino , Camundongos Endogâmicos C57BL , Ácido Hialurônico/farmacologia , Receptores de Hialuronatos/metabolismo , Receptores de Hialuronatos/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Linhagem Celular , Fosfatidilinositol 3-Quinases/metabolismo , Transdução de Sinais/efeitos dos fármacos , Traumatismo por Reperfusão/terapia , Traumatismo por Reperfusão/tratamento farmacológico , Traumatismo por Reperfusão/patologia , Traumatismo por Reperfusão/metabolismo , Modelos Animais de Doenças , Apoptose/efeitos dos fármacos
2.
Vaccine ; 42(26): 126395, 2024 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-39342902

RESUMO

OBJECTIVE: To validate the Chinese version of the vaccine conspiracy beliefs scale (VCBS) and to investigate and compare its correlates among healthcare workers and non-healthcare workers during COVID-19 pandemic. METHODS: From March 19 to April 05, 2021, a nationwide cross-sectional online survey was administered to Chinese individuals aged 16 and above. The study aimed to assess the reliability and validity of the Chinese version of the VCBS. A binary logistic or linear regression model, along with mediation analysis, was employed to explore the relationship between vaccine conspiracy beliefs and its correlates among healthcare workers. RESULTS: The VCBS demonstrated robust psychometric properties, showing satisfactory results from both exploratory and confirmatory factor analyses, strong internal consistency (KR-20 = 0.947), and calibration validity. Measurement invariance (MI) was also observed. Additionally, individuals' vaccine conspiracy beliefs, as measured by the VCBS, mediate the relationship between occupation and COVID-19 vaccine hesitancy (95 % CI [-2.229, -1.060]), as well as COVID-19 (95 % CI [0.096, 0.304]), influenza (95 % CI [0.075, 0.304]), and HPV (95 % CI [0.009, 0.236]) vaccination uptakes. CONCLUSION: The Chinese adaptation of the VCBS proves adept at assessing vaccine conspiracy beliefs among Chinese individuals aged 16 and above. Notably, the healthcare workers are less likely to exhibit COVID-19 vaccine hesitancy and are more likely to receive COVID-19, influenza, and HPV vaccines. This tendency is partly related to their weaker conspiracy beliefs about vaccination.

3.
Front Psychol ; 14: 1215209, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37941753

RESUMO

This study sought to validate the psychometric properties of the Health Regulatory Focus Scale (HRFS), emphasizing its manifestation and association with personality traits in a Chinese context. Originally developed by Ferrer, the HRFS gauges individuals' inclinations either to avoid negative health outcomes (prevention focus) or achieve positive health outcomes (promotion focus). Our cross-sectional analysis involved a diverse sample of 652 Chinese participants, averaging 39.6 years in age (SD = 9.39). Data were analyzed using SPSS and AMOS, and both exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were employed to assess the HRFS's factor structure. Additionally, we evaluated convergent and discriminant validity, criterion-related validity, internal consistency reliability, and test-retest reliability. The CFA results (CFI = 0.985, TLI = 0.971, RMSEA = 0.059, and SRMR = 0.047), combined with McDonald's omega value (0.916) and the test-retest correlation coefficient (0.78) for the HRFS, underscore its robust construct validity and reliability. Furthermore, the promotion dimension of the HRFS exhibited significant positive correlations with all dimensions of the Chinese Adjectives Short Scale of Big-Five Factor Personality (BFFP-CAS-S). In conclusion, the HRFS's Chinese adaptation offers a reliable and valid instrument for assessing health regulatory focus.

4.
Int Urol Nephrol ; 46(8): 1673-9, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24973203

RESUMO

PURPOSE: The aim of this study was to elucidate the role of neutrophil gelatinase-associated lipocalin (NGAL) in regulating apoptosis of tubular epithelial cells in a hypoxia-reperfusion model. METHODS: A hypoxia-reperfusion model was established with NRK-52E cells to assess apoptosis and cell cycle progression after the addition of NGAL. We investigated the expression of four apoptosis factors, Bcl-2, Bax, Fas and FasL, as well as the expression level of two NGAL receptors, 24p3R and megalin, by both Western blot and real-time PCR. RESULTS: NGAL induced cell proliferation and reduced apoptosis by regulating four apoptosis factors Bcl-2, Bax, Fas and FasL. Western blot demonstrated that the two NGAL receptors, 24p3R and megalin, were increased after hypoxia-reperfusion, which was reduced by exogenous NGAL. Moreover, overexpression of the two receptors induced the expression of the anti-apoptotic factor Bcl-2 and reduced the expression of pro-apoptotic Bax, Fas and FasL. CONCLUSIONS: These findings indicate that NGAL reduces apoptosis by regulating the four apoptosis factors Bcl-2, Bax, Fas and FasL through its two receptors 24p3R and megalin. These results also suggest that ectopic expression of NGAL in renal cells might provide a therapeutic strategy in ischemia-reperfusion by reducing apoptosis and promoting renal cell proliferation.


Assuntos
Proteínas de Fase Aguda/farmacologia , Apoptose/efeitos dos fármacos , Hipóxia Celular/fisiologia , Células Epiteliais/efeitos dos fármacos , Lipocalinas/farmacologia , Proteínas Proto-Oncogênicas/farmacologia , RNA Mensageiro/metabolismo , Traumatismo por Reperfusão/metabolismo , Animais , Linhagem Celular , Proliferação de Células , Regulação para Baixo/efeitos dos fármacos , Células Epiteliais/fisiologia , Proteína Ligante Fas/genética , Proteína Ligante Fas/metabolismo , Túbulos Renais , Lipocalina-2 , Proteína-2 Relacionada a Receptor de Lipoproteína de Baixa Densidade/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Ratos , Receptores de Superfície Celular/metabolismo , Regulação para Cima/efeitos dos fármacos , Proteína X Associada a bcl-2/genética , Proteína X Associada a bcl-2/metabolismo , Receptor fas/genética , Receptor fas/metabolismo
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