RESUMO
We constructed a bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.
Assuntos
Mapeamento de Sequências Contíguas , Drosophila melanogaster/genética , Genoma , Animais , Centrômero/genética , Cromatina/genética , Cromossomos Bacterianos/genética , Clonagem Molecular , Impressões Digitais de DNA , Eucromatina , Biblioteca Gênica , Genes de Insetos , Marcadores Genéticos , Vetores Genéticos , Hibridização In Situ , Sequências Repetitivas de Ácido Nucleico , Mapeamento por Restrição , Análise de Sequência de DNA , Sitios de Sequências Rotuladas , Telômero/genéticaRESUMO
The fly Drosophila melanogaster is one of the most intensively studied organisms in biology and serves as a model system for the investigation of many developmental and cellular processes common to higher eukaryotes, including humans. We have determined the nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophila genome using a whole-genome shotgun sequencing strategy supported by extensive clone-based sequence and a high-quality bacterial artificial chromosome physical map. Efforts are under way to close the remaining gaps; however, the sequence is of sufficient accuracy and contiguity to be declared substantially complete and to support an initial analysis of genome structure and preliminary gene annotation and interpretation. The genome encodes approximately 13,600 genes, somewhat fewer than the smaller Caenorhabditis elegans genome, but with comparable functional diversity.
Assuntos
Drosophila melanogaster/genética , Genoma , Análise de Sequência de DNA , Animais , Transporte Biológico/genética , Cromatina/genética , Clonagem Molecular , Biologia Computacional , Mapeamento de Sequências Contíguas , Sistema Enzimático do Citocromo P-450/genética , Reparo do DNA/genética , Replicação do DNA/genética , Drosophila melanogaster/metabolismo , Eucromatina , Biblioteca Gênica , Genes de Insetos , Heterocromatina/genética , Proteínas de Insetos/química , Proteínas de Insetos/genética , Proteínas de Insetos/fisiologia , Proteínas Nucleares/genética , Biossíntese de Proteínas , Transcrição GênicaRESUMO
A comparative analysis of the genomes of Drosophila melanogaster, Caenorhabditis elegans, and Saccharomyces cerevisiae-and the proteins they are predicted to encode-was undertaken in the context of cellular, developmental, and evolutionary processes. The nonredundant protein sets of flies and worms are similar in size and are only twice that of yeast, but different gene families are expanded in each genome, and the multidomain proteins and signaling pathways of the fly and worm are far more complex than those of yeast. The fly has orthologs to 177 of the 289 human disease genes examined and provides the foundation for rapid analysis of some of the basic processes involved in human disease.
Assuntos
Caenorhabditis elegans/genética , Drosophila melanogaster/genética , Genoma , Proteoma , Saccharomyces cerevisiae/genética , Animais , Apoptose/genética , Evolução Biológica , Caenorhabditis elegans/química , Caenorhabditis elegans/fisiologia , Adesão Celular/genética , Ciclo Celular/genética , Drosophila melanogaster/química , Drosophila melanogaster/fisiologia , Proteínas Fúngicas/química , Proteínas Fúngicas/genética , Genes Duplicados , Doenças Genéticas Inatas/genética , Genética Médica , Proteínas de Helminto/química , Proteínas de Helminto/genética , Humanos , Imunidade/genética , Proteínas de Insetos/química , Proteínas de Insetos/genética , Família Multigênica , Neoplasias/genética , Estrutura Terciária de Proteína , Saccharomyces cerevisiae/química , Saccharomyces cerevisiae/fisiologia , Transdução de Sinais/genéticaRESUMO
OBJECTIVE: To evaluate a nurse-led attendance allowance screening service in General Practice. DESIGN: Intervention study. SETTING: One General Practice located in two sites in deprived areas in the East-End of Glasgow (Carstairs Deprivation Index-6 and 4). PARTICIPANTS: Participants aged >64 y who in the nurses clinical judgment appeared to be physically or mentally frail were opportunistically recruited over a 12-week period by community nurses (health visitors, district nurses and practice nurses). A Welfare Rights Officer (WRO) contacted all potential underclaimers by telephone and offered a home visit in order to assess for unclaimed benefits. MAIN OUTCOME MEASURE: Total unclaimed attendance allowance and linked benefits. RESULTS: Thirty-seven of the original 86 participants plus four relatives were found not to be claiming the benefit payments that they were entitled to. Referral to the Department of Social Security (DSS) revealed unclaimed benefits to a total of: pound sterling112 893.00of this pound sterling95 306.00 is on a recurrent annualized basis and pound sterling17 587.00 as lump sums. CONCLUSIONS: A community nurse-led attendance allowance screening service combined with a home visit by a WRO was an efficient and highly effective model for maximising the income of the frail elderly. This model could contribute to reducing the increasing number of pensioners living below the poverty line.
Assuntos
Enfermagem em Saúde Comunitária , Idoso Fragilizado , Avaliação em Enfermagem , Áreas de Pobreza , Assistência Pública , Idoso , Idoso de 80 Anos ou mais , Medicina de Família e Comunidade , Humanos , Pessoa de Meia-Idade , Seguridade Social , Reino UnidoRESUMO
For nearly a century, genetic analysis in Drosophila melanogaster has been a powerful tool for analyzing gene function, yet Drosophila lacks the molecular genetic mapping tools that recently have revolutionized human, mouse, and plant genetics. Here, we describe the systematic characterization of a dense set of molecular markers in Drosophila by using a sequence tagged site-based physical map of the genome. We identify 474 biallelic markers in standard laboratory strains of Drosophila that span the genome. Most of these markers are single nucleotide polymorphisms and sequences for these variants are provided in an accessible format. The average density of the new markers is one per 225 kb on the autosomes and one per megabase on the X chromosome. We include in this survey a set of P-element strains that provide additional use for high-resolution mapping. We show one application of the new markers in a simple set of crosses to map a mutation in the hedgehog gene to an interval of <1 Mb. This new map resource significantly increases the efficiency and resolution of recombination mapping and will be of immediate value to the Drosophila research community.