Investigating the effect of music on labor pain and progress in the active stage of first labor.

UNLABELLED: DESIGN AND PURPOSE: The purpose of this study is to investigate the effects of music-therapy on labor pain and progress in parturient primipara. Music-therapy during labor increases tolerance to pain; decreasing anxiety, it increases paturition and uterus activity and shorten labor duration. SUBJECTS AND METHODS: The subjects of this research were 30 women, selected voluntarily and they have been put in two experimental and control group. This research has been conducted in the form of pre-test and post-test design. The experimental group listened to a relaxing music for 30 minutes in each hour for a two-hour period a nd the control group was not exposed to music during this period. For the purpose of gathering data in both groups, the pain scales (verbal, numeric and visual) was used to measure pain. The independent variable in this research is relaxing music and the dependent variables are the pain level and delivery progress. FINDINGS: The independent t for sensations of pain in the experimental and control group before intervention has been (p = 0.875) 0.601 in numeric and visual pain and (p < 0.01) 2.92 in verbal pain, and one hour after intervention, it has been (p < 0.0001) 8.527 in visual and numeric pain and (p < 0.0001) 11.824 in verbal pain. Also, the equal value of independent t for the duration of delivery in control group before and after intervention shows that music has not had any effect on the rate of serotonin. CONCLUSION: The results of statistical analysis show the effect of music on the decrease of sensation of pain in the experimental group as compared with the control group.

Short-term in-vitro culture of goat enriched spermatogonial stem cells using different serum concentrations.

PURPOSE: To investigate the effect of serum supplementing on short-term culture, fate determination and gene expression of goat spermatogonial stem cells (SSCs). METHODS: Crude testicular cells were plated over Datura-Stramonium Agglutinin (DSA) for 1 h, and non-adhering cells were cultured in the presence of different serum concentrations (1, 5, 10, and 15%) for 7 days in a highly enriched medium initially developed in mice. Colonies developed in each group were used for the assessment of morphology, immunocytochemistry, and gene expression. RESULTS: Brief incubation of testicular cells with DSA resulted in a significant increase in the number of cells that expressed the germ cell marker (VASA). The expression of THY1, a specific marker of undifferentiated spermatogonia, was significantly higher in colonies developed in the presence of 1% rather than 5, 10 and 15% serum. CONCLUSION: Goat SSCs could proliferate and maintain in SSC culture media for 1 week at serum concentrations as low as 1%, while higher concentrations had detrimental effects on SSC culture/expansion.

Excited state electric dipole moment of nile blue and brilliant cresyl blue: a comparative study.

A solvatochromic study on the photophysical properties of two cationic oxazine dyes (brilliant cresyl blue and nile blue) was carried out. The electronic absorption and emission spectra of the dyes were recorded in various organic solvents with different polarity. The ground and the excited state dipole moments of the dyes were estimated from solvatochromic shift method. The solvent dependent spectral shifts in absorption and fluorescence spectra were analyzed by the Katritzky and Kamlet-Taft multi-parameter scales. This work is characterized by detailed quantitative studies on the nature and extent of solvent-solute interactions.

A comparative study on the aggregate formation of two oxazine dyes in aqueous and aqueous urea solutions.

The visible absorption spectra of two oxazine dyes, brilliant cresyl blue and nile blue, in aqueous and aqueous urea solutions (0.01-1 M) were studied as a function of the dye concentration. Due to structural similarity of the investigated dyes, their spectral features were compared. The dimerization behavior of the dyes in water with or without urea was analyzed in terms of monomer-dimer equilibrium. The nature and structure of the interacting pairs for these dyes were discussed using the exciton theory. The dimerization constant values of the dyes in aqueous and aqueous urea solutions were calculated. For both the dyes in aqueous urea solutions, a reduction in dimer formation was observed. The excitonic parameters were compared with those of oxazine-1, which is structurally similar to the investigated dyes.