RESUMO
OBJECTIVE: Keeping a wound moist can allow effective and rapid healing, and it can control the formation of scabs, thereby allowing cell proliferation and epithelial formation. When regularly changing a dressing, thermosensitive hydrogel as a moist dressing does not cause a secondary wound from adhesion. The main aim of this study was to evaluate the effect of a new sprayable thermosensitive hydrogel on wound healing. METHOD: The hydrophobic N-acetyl group of chitin was removed by microwave reaction with lye until the degree of acetylation was 60%, followed by reaction with propylene oxide to obtain hydroxypropyl chitin (HPCH) with a degree of substitution of 40%. After mixing HPCH with fish scale collagen (FSC), a thermosensitive hydrogel with a gel temperature of 26.5°C was obtained. Ampelopsis brevipedunculata extracts (ABE), which have been found to accelerate wound repair and improve healing, were added. HPCH/FSC is not toxic to the mouse L929 cell line and forms a hydrogel at body surface temperature. It can be easily sprayed on a wound. The HPCH/FSC has a three-dimensional network porous structure with a swelling ratio of 10.95:1 and a water vapour transmission rate of 2386.03±228.87g/m2/day; it can facilitate the penetration of water and air, and promote absorption of wound exudate. Wound repair was performed on five Sprague-Dawley rats. Each rat had three wounds, which were treated with medical gauze, HPCH/FSC and HPCH/FSC/ABE, respectively. RESULTS: The wounds in the HPCH/FSC/ABE group recovered the fastest in vivo, the mature wound site was smoother, the re-epithelialisation was even and thicker, and the angiogenesis developed rapidly to the mature stage. CONCLUSION: In this study, HPCH/FSC/ABE thermosensitive hydrogel was shown to effectively accelerate wound healing and was convenient for practical application.
Assuntos
Ampelopsis , Hidrogéis , Camundongos , Ratos , Animais , Hidrogéis/farmacologia , Quitina/química , Quitina/farmacologia , Ratos Sprague-Dawley , Cicatrização , Colágeno/farmacologiaRESUMO
Silver nanoparticles (AgNPs) synthesized from Aloe vera extract exhibited a pronounced antibacterial effect, while the Ampelopsis brevipedunculata extract (ABE) showcased a high antioxidant capacity for wound healing. Spherical AgNPs with a particle size of 28.82 nm crystallized in a face-centered-cubic lattice. AgNPs/polyvinyl alcohol (PVA) and ABE/polycaprolactone (PCL) underwent electrospinning to produce coaxial and electrosprayed nanofibers, respectively. The developed coaxial nanofibers demonstrated a strain of 159%, a Young's modulus of elasticity of 7080.14 kPa, a 3.9-fold swelling ratio, a water contact angle of 38.91°, characteristic hydrophilicity, and an adequate water vapor transmission rate of 2272 g/m2/day. ABE exhibited no cytotoxicity to L929 cells and induced a twofold increase in the cell migration rate. Upon applying the developed coaxial nanofiber on an in vivo rat model with a 9 mm wound diameter, the wound rapidly and completely healed within 10 days, with a healing speed 60% greater than that of the control group. Histopathological analysis revealed that the coaxial group did not exhibit inflammation, showed complete epithelization, and featured a well-arranged deposition of collagen on the 10th day.