Therapeutic potential of Mucuna pruriens (Linn.) on high-fat diet-induced testicular and sperm damage in rats.

Objective: Mucuna pruriens Linn., a leguminous plant, is identified as a herbal medicine for improving fertility-related disorders in the alternative and complementary systems of medicine. The study was focused on evaluating the therapeutic potential of M. pruriens on testis and sperm parameters in a high-fat-induced hypercholesterolemia model. Materials and Methods: Male rats were divided as normal-control rats (NCR); normal-control rats + M.pruriens (200 mg/kg b.w. of ethanolic extract of M. pruriens seed) treated (NCRD); hypercholesterolemic rats (HCR) and hypercholesterolemic rats + M. pruriens (HCRD). Groups were further divided into three post-exposure periods (subgroups) of 9, 18, and 36 days, and the progressive changes in testis histology and sperm were analyzed. Results: The study showed a significant impairment in testicular histoarchitecture, depletion of antioxidant enzyme levels, increased oxidative stress and lipid peroxidation in the HCR group. The study indicated severe structural and functional damage in sperm parameters and diminished chromatin integrity in the HCR group. In the HCR rats, the follicular stimulating hormone (FSH) and luteinizing hormone (LH) and testosterone were significantly reduced. There was a significant improvement in sperm parameters and testis histology in the HCRD group. Conclusion: The study reveals the potential efficacy of M. pruriens to improve spermatogenesis, sperm parameters and hormone levels in hypercholesterolemic rats.

Indirect calculation of LDL using thirteen equations in Pakistani population.

BACKGROUND: Owing to the atherogenic properties, low density lipoprotein cholesterol (LDL-C) is the primary target for treatment and diagnosis of cardiovascular diseases (CVDs), hence accurate measurement of LDL-C is critical. Despite the availability of direct measurement assays for LDL-C, it is routinely calculated by Friedewald equation in clinical settings in Pakistan mostly due to financial constraints. However, the validity of this equation is impacted by several factors, therefore several other equations have been developed for the calculation of LDL-C. MATERIALS AND METHODS: LDL-C of 39,385 individuals measured directly by homogenous assays (dLDL) was compared with LDL-C calculated by thirteen equations (cLDL-C). Stratifications based on different lipids i.e., triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL) were made to check the validity of these equations across all ranges of lipid profile. The correlation and median difference between dLDL and cLDL-C was statistically analyzed. RESULTS: Overall Teerakanchana equation displayed a strong positive correlation (ρ = 0.967) and least median difference (-8.81) with dLDL, followed by Martin equation (ρ = 0.967). For higher TG ranges (>500 mg/dL), Teerakanchana equation had the least median difference (1.31) and a strong correlation (ρ = 0.800). CONCLUSION: Our data suggest that Teerakanchana equation may be employed as an alternative to Friedewald equation for Pakistani population.

Optimization of the Fine to Coarse Aggregate Ratio for the Workability and Mechanical Properties of High Strength Steel Fiber Reinforced Concretes.

High-strength concrete is used to provide quality control for concrete structures, yet it has the drawback of brittleness. The inclusion of fibers improves the ductility of concrete but negatively affects the fresh properties of fiber-reinforced concrete. The effects of different fine to coarse aggregate ratios on the fresh and hardened properties of steel fiber reinforced concrete were investigated in this study. Mixtures were prepared with various fine to coarse aggregate (FA/CA) ratios incorporating 1% steel fiber content (by volume) at constant water to cement ratio. The workability, unit weight, and temperature of the concrete in the fresh state, and the mechanical properties of steel-fiber-reinforced concrete (SFRC) were investigated. The inclusion of fiber in concrete influenced the mobility of concrete in the fresh state by acting as a barrier to the movement of coarse aggregate. It was observed that the concrete with an FA/CA ratio above 0.8 showed better flowability in the fresh state, whilst an above 0.9 FA/CA ratio requires excessive superplasticizer to maintain the flowability of the mixtures. The compressive and flexural strength of SFRC increased with an increase in the FA/CA ratio by around 10% and 28%, respectively. Experimental values of compressive strength and flexural strength showed good agreement, however, modulus of elasticity demonstrated slightly higher values. The experimentally obtained measurements of the mechanical properties of SFRC conformed reasonably well with the available existing prediction equations, and further enabled establishing predictive isoresponse interactive equations within the scope of the investigation domain.