The influence of inhomogeneous adhesion on the detachment dynamics of adhering cells.

The adhesion of cells to surfaces plays a crucial role in processes related to motility and tissue growth. Nonspecific interactions with a surface, e.g., by electrostatic or van der Waals forces, can complement specific molecular interactions and can themselves support strong adhesion. In order to understand the mechanism by which cells establish an adhesive interface in the absence of specific proteins, we have studied the detachment kinetics of monocytic cells from glass surfaces coated with poly-L-lysine. We exposed adhering cells to a shear flow and studied their deformation and detachment trajectories. Our experiments reveal that between 20 and 60 parallel membrane tethers form prior to detachment from the surface. We propose that the extraction of tethers is the consequence of an inhomogeneous adhesion interface and model the detachment mechanism as the dynamic extrusion of cooperatively loaded tethers. In our model, individual tethers detach by a peeling process in which a zone of a few nanometers is loaded by the externally applied force. Our findings suggest that the formation of an inhomogeneous non-specific adhesion interface between a cell and its substrate gives rise to more complex dynamics of detachment than previously discussed.

Probing the cell membrane by magnetic particle actuation and Euler angle tracking.

The mechanical properties of the cell membrane and the subjacent actin cortex are determinants of a variety of processes in immunity and cell division. The lipid bilayer itself and its connection to the actin cortex are anisotropic. An accurate description of the mechanical structure of the cell membrane and the involved dynamics therefore necessitates a measurement technique that can capture the inherent anisotropy of the system. Here, we combine magnetic particle actuation with rotational and translational particle tracking to simultaneously measure the mechanical stiffness of monocytic cells in three rotational and two translational directions. When using particles that bind via integrins to the cell membrane and the subjacent cortex, we measured an isotropic stiffness and a characteristic power-law dependence of the shear modulus on the applied frequency. When using particles functionalized with immunoglobulin G, we measured an anisotropic stiffness with a 10-fold-reduced value in one dimension. We suggest that the observed reduced stiffness in the plane of the cell membrane is caused by a local detachment of the lipid bilayer from the subjacent cytoskeletal cortex. We expect that our technique will enable new insights into the mechanical properties of the cell membrane that will help us to better understand membrane processes such as phagocytosis and blebbing.

A method for time-resolved measurements of the mechanics of phagocytic cups.

The internalization of matter by phagocytosis is of key importance in the defence against bacterial pathogens and in the control of cancerous tumour growth. Despite the fact that phagocytosis is an inherently mechanical process, little is known about the forces and energies that a cell requires for internalization. Here, we use functionalized magnetic particles as phagocytic targets and track their motion while actuating them in an oscillating magnetic field, in order to measure the translational and rotational stiffnesses of the phagocytic cup as a function of time. The measured evolution of stiffness reveals a characteristic pattern with a pronounced peak preceding the finalization of uptake. The measured stiffness values and their time dependence can be interpreted with a model that describes the phagocytic cup as a prestressed membrane connected to an elastically deformable actin cortex. In the context of this model, the stiffness peak is a direct manifestation of a previously described mechanical bottleneck, and a comparison of model and data suggests that the membrane advances around the particle at a speed of about 20 nm s(-1). This approach is a novel way of measuring the progression of emerging phagocytic cups and their mechanical properties in situ and in real time.