Kidney Mesenchymal stem cells alleviate cisplatin-induced kidney injury and apoptosis in rats.

OBJECTIVE: This experiment was designed to demonstrate Mesenchymal stem cells (MSCs) derived from kidney can alleviate cisplatin-induced kidney injury and renal cell apoptosis through paracrine pathway. METHODS: Firstly, MSCs were isolated from kidney of young rats, and their surface-specific markers were identified by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and immunofluorescence staining. Self-renewal ability of Kidney Mesenchymal Stem Cells (KMSCs) was observed by cell counting and 5-Bromo-2'-deoxyuridine (BrdU) fluorescence staining. KMSCs at logarithmic growth stage were traced and injected into rat through tail vein. RESULTS: The results showed that KMSCs homed in the kidney tissues, decreased the secretion of inflammatory factors (CRP, TNFα, IL-1ß, IL-6), and alleviated renal function. Hematoxylin and Eosin (H＆E), Masson and Periodic Acid-silver Methenamine (PASM) staining showed that KMSCs could alleviate pathological damage in rats. Terminal Deoxynucleotidyl Transferase mediated dUTP Nick-End Labeling (TUNEL) assay showed that KMSCs could reduce the apoptosis of rat kidney cells induced by cisplatin. Finally, Immunohistochemistry (IHC) results showed that cisplatin could induce higher expression of the pro-apoptotic protein Bax and lower expression of anti-apoptotic Bcl-2 in kidney tissues. However, KMSCs could reverse the pro-apoptotic effect of cisplatin on kidney cells and improve the survival rate of rats. CONCLUSIONS: In conclusion, KMSCs were successfully isolated from kidney tissues, and KMSCs have therapeutic effects on rat kidney injury induced by cisplatin.

The Detection of Toxoplasma gondii in Wild Rats (Rattus norvegicus) on Mink Farms in Shandong Province, Eastern China.

Toxoplasma gondii is a worldwide distributed zoonotic pathogen that threatens public health. However, there have been limited data for T. gondii infection in wild rats (Rattus norvegicus) in China. In the present study, a total of 227 wild rats were captured from three mink farms to investigate T. gondii infection in Shandong Province, eastern China. The DNA was extracted from 25 mg rats' brain tissues and subjected to a PCR amplification by targeting to the T. gondii B1. In 227 wild rat samples, 18 samples (7.93%) were positive for T. gondii. Then, the positive samples were further genotyped based on eight genetic markers, including eight nuclear loci (SAG1, 5'-SAG2, and 3'-SAG2, alternative SAG2, SAG3, GRA6, c29-2, and L358) and an apicoplast locus (Apico) by using the multilocus PCR-restriction fragment length polymorphism technology. Of these samples, eight were genotyped at nine nuclear loci, and two were genotyped at eight nuclear loci, forming three known genotypes (ToxoDB no. 43, ToxoDB no. 91, and ToxoDB no. 189) and two new genotypes. The closest ToxoDB genotypes were observed in wild rats, suggesting the differences in the population structure of the T. gondii between breed farm animals and wild rats. These data revealed the genetic variability of T. gondii in wild rats on mink farms in Shandong Province, with possible implication for public health.

First Report on Toxoplasma gondii Seroprevalence in Free-Ranging Pigs in Northeastern China.

INTRODUCTION: Toxoplasma gondii is the pathogen of toxoplasmosis, which affects a wide range of animal species and humans. MATERIALS AND METHODS: In the present study, a serological investigation was undertaken to determine the seroprevalence of T. gondii infection in free-ranging pigs in northeastern China. A total of 806 serum samples were collected from free-ranging pigs in 6 cities in Liaoning, Jilin, and Heilongjiang Provinces. RESULTS: Among them, 163 serum samples (20.22%) were detected T. gondii positive by indirect hemagglutination test (IHA). In each administrative district, the prevalence ranges from 12.99 to 22.22%. It was found that sample collection year and season were associated with T. gondii infection. Samples collected in 2015 and 2016 had had higher positive rate compared to those collected in 2017. Samples collected in summer and autumn also have higher positive rate than those in winter. CONCLUSION: This is the first report on T. gondii seroprevalence in free-ranging pigs in northeastern China, which adds the epidemiological data of T. gondii infection in free-ranging pigs in China. These results indicated that free-ranging pigs in Liaoning, Jilin, and Heilongjiang Provinces are exposed to T. gondii, which is a significant threat to local pig industry as well as public health. It is necessary to take relevant comprehensive measures to prevent and control T. gondii infection in free-ranging pigs in these regions.