Transcriptome profiling reveals the roles of pigment formation mechanisms in yellow Paeonia delavayi flowers.

The yellow colour of ornamental varieties of tree peony originated from Paeonia delavayi. However, but P. delavayi and Paeonia suffruticosa belong to different subgroups, so hybridization is difficult and results in a long breeding cycle. However, no comprehensive transcriptomic profiling has focused on the colour formation mechanisms of yellow tree peony petals. Analysing the colour formation mechanism of yellow petals in P. delavayi is very important for directional molecular breeding. In this study, the transcriptional map of yellow pigment development in petals was used to analyse the mechanism of petal colour formation. We analysed the genes related to the metabolism of flavonoids and carotenoids and the transcription factors (TFs) involved in P. delavayi var. lutea (pure yellow individual) yellow pigment development using transcriptome sequence profiling. Transcriptome sequence profiles revealed three and four differentially expressed transcripts (DETs) involved in flavonoid biosynthesis and carotenoid biosynthesis, respectively. An analysis of DETs in the flavonoid pathway showed that chalcone synthase (CHS) and chalcone 2´-glucosyltransferases (THC2'GT) act in synergy to synthesize isosalipurposide (ISP). CHS and flavonol synthase (FLS) synergistically synthesize quercetin and kaempferol. DEG analysis of the carotenoid pathway revealed that phytoene synthase (PSY), carotenoid isomerase (CRTISO) and ß-carotene hydroxylases (CHYB) play a key role in regulating lutein formation, and carotenoid cleavage dioxygenase (CCD) plays an important role in the degradation of carotenoids. These two pathways may be regulated by TF families such as bHLH, ARF, and MYB. The results of the transient overexpression of genes showed that CHS and CHI are regulated by PdMYB2. In this study, the molecular mechanism of ISP synthesis was analysed in depth, and the complete metabolic pathway of carotenoids in Paeonia L. was reported for the first time. By studying the formation mechanism of yellow pigment in P. delavayi petals, a breeding strategy for improving flavonol and carotenoid contents and reducing anthocyanin synthesis by genetic engineering was suggested.

Visual ratiometric fluorescence sensing of L-lactate in sweat by Eu-MOF and the design of logic devices.

Using 1, 4-H2NDC as ligand and Eu as the center metal, the lanthanide MOF Eu-NDC was synthesized by hydrothermal method. The material showed a fast ratiometric response to L-lactate, and the fluorescence of the material varied from red to blue with the growth of lactate concentration, which can be used as a fluorescent sensor for L-lactate in sweat. The sensor exhibited good fluorescence stability to interfering components in human sweat and good detection limits for lactate in artificial sweat. Based on this, a visualized molecular logic gate that can monitor sweat lactate levels was constructed, and the material's characteristic of showing different colors with lactate concentration changes was used to indicate possible hypoxia during exercise, opening a new path for combining sweat lactate monitoring with smart molecular devices.

Eu3+-Functionalized MOFs for the simple and rapid 5-Hydroxymethylfurfural determination in food.

5-Hydroxymethylfurfural (5-HMF) is an important product of the Maillard reaction and can be used as a quality indicator of food. 5-HMF has been found in studies to be harmful to human health. In this study, a highly selective and anti-interference fluorescent sensor Eu@1 is constructed based on Eu3+-functionalized Hf-based MOF for monitoring 5-HMF in a variety of food products. Eu@1 shows high selectivity, low LOD (8.46 µM), fast response time, and repeatability for 5-HMF. More importantly, after adding 5-HMF to milk, honey and apple juice samples, the probe Eu@1 is proved to be successfully in sensing 5-HMF in the above food samples. Therefore, this study provides a dependable and efficient alternative for the detection of 5-HMF in food samples.

Correction: A double responsive fluorescent platform for sensing heavy metal ions based on a dual-emitting fluorescent covalent organic framework hydrogel film.

Correction for 'A double responsive fluorescent platform for sensing heavy metal ions based on a dual-emitting fluorescent covalent organic framework hydrogel film' by Yinghua Jia et al., Dalton Trans., 2022, 51, 14352-14358.

A double responsive fluorescent platform for sensing heavy metal ions based on a dual-emitting fluorescent covalent organic framework hydrogel film.

A new luminescent hybrid material with dual-emission centers (Eu@Dye@TpDq hydrogel) based on a covalent organic framework (COF) has been successfully prepared. First, we designed a dye (coumarin) modified COF (TpDq) hybrid fluorescent material (Dye@TpDq), and then the material was formed into a hydrogel film, with an Eu3+ ion being introduced as another luminescent center into the material during the cross-linking process. As a dual-emission fluorescent platform, it exhibits a double-response towards different heavy metal ions: a ratiometric identification response to Cu(II), and a turn-off fluorescence response to Cr(VI). This research not only introduces a novel synthesis of Eu3+-functionalized double emitting COF-based hydrogels, but also inspires the development of COF-based multifunctional analytical tools.

Eu3+-ß-diketone functionalized covalent organic framework hybrid material as a sensitive and rapid response fluorescent sensor for glutaraldehyde.

A covalent organic framework (named as TpDq) linked by ß-ketoamine was prepared by imine condensation reaction with 1,3,5-triformylphloroglucinol (TFP) and 2,6-diaminoanthraquinone (DAAQ) as building blocks. Via employing a functionalized modification strategy, a new lanthanide complex Eu3+-ß-diketone functionalized covalent organic framework hybrid material, Eu-TTA@TpDq (TTA = 2-thenoyltrifluoroacetone), has been synthesized. After post-synthetic modification (PSM), the shape and structure of the parent framework is well preserved and the modified material shows remarkable luminescence properties. Based on this, we designed it as a fluorescent probe and tried to use it to sense common aldehydes. The results indicate that Eu-TTA@TpDq exhibits a turn-off response toward glutaraldehyde which can distinguish from other common aldehydes. The fluorescent probe has the advantages of reusability, pH stability (4.50-8.52), fast luminescence response (<1 min) and low detection limit. The linear range of this method was 0-100 µM; the detection limit was 4.55 µM; the relative standard deviation was 2.16%. Furthermore, it has broad application prospect in both practical sensing of glutaraldehyde in water environment and simple detection of glutaraldehyde vapor. In addition, we preliminarily discussed the possible sensing mechanism.